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白蛋白/细胞角蛋白19启动子调控的红绿双色荧光蛋白报告载体的构建及其在肝干细胞分化研究中的应用

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第二军医大学学报 2005年第3期26卷 237-239页

作者：李文林苏娟陶欣荣 joseph T Lau 胡以平第二军医大学基础医学部细胞生物学教研室 The Department of Molecular and Cellular Biology Roswell Park Cancer InstituteElm and Carlton StreetsBuffaloNY 14263USA

目的:利用报告基因监测肝干细胞的分化走向。方法:通过PCR从小鼠基因组中克隆了细胞角蛋白19 启动子片段,构建了细胞角蛋白19启动子调控的绿色荧光蛋白(pCK19 EGFP)和白蛋白启动子调控的红色荧光蛋白报告载体(pAlbD sRed),并利用报告载... 详细信息

目的:利用报告基因监测肝干细胞的分化走向。方法:通过PCR从小鼠基因组中克隆了细胞角蛋白19 启动子片段,构建了细胞角蛋白19启动子调控的绿色荧光蛋白(pCK19 EGFP)和白蛋白启动子调控的红色荧光蛋白报告载体(pAlbD sRed),并利用报告载体标记的肝干细胞(liver epithelial progenitor cells,LEPCs)观察了悬浮培养时LEPCs的分化去向。结果:白蛋白/细胞角蛋白19启动子调控的红绿双色荧光蛋白报告载体可以实时地显示肝原始细胞在不同的诱导环境下的分化走向。结论:双色荧光蛋白报告载体的成功构建为研究LEPCs的分化、筛选可诱导LEPCs定向分化的分子提供了便捷的工具。

关键词：肝干细胞细胞分化白蛋白细胞角蛋白19 启动子红色荧光蛋白绿色荧光蛋白基因.报告

表达红色荧光蛋白DsRed报告基因的逆转录病毒载体的构建和在肝干细胞体内示踪中的应用

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第二军医大学学报 2005年第3期26卷 240-242页

目的:建立表达红色荧光蛋白DsRed的逆转录病毒载体,并对肝原始细胞进行体外标记和体内示踪研究。方法:将pDsRed1 1载体中的DsRed片段克隆到逆转录病毒载体pLNCG C1中构建了pLNCG DsRed载体。将该载体导入Phoenix包装细胞,进而采用乒乓... 详细信息

目的:建立表达红色荧光蛋白DsRed的逆转录病毒载体,并对肝原始细胞进行体外标记和体内示踪研究。方法:将pDsRed1 1载体中的DsRed片段克隆到逆转录病毒载体pLNCG C1中构建了pLNCG DsRed载体。将该载体导入Phoenix包装细胞,进而采用乒乓转染的方法感染包装细胞PT67,获得高滴度稳定产毒的PT67 细胞系。结果:利用PT67 细胞系产生的病毒上清可以高效的感染体外培养的肝原始细胞系。将这些DsRed标记的肝原始细胞移植到小鼠损伤肝脏中,4周后可以在再生肝脏中清晰地观察到具有红色荧光的外源细胞。结论:由于肝脏组织没有红色自发荧光,用DsRed标记肝原始细胞进行肝内示踪时背景干扰小。

关键词：红色荧光蛋白基因.报告逆转录病毒载体肝干细胞

舒更葡糖钠用于ASA分级Ⅲ或Ⅳ级高风险手术患者安全性的评价:随机双盲临床研究

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中华麻醉学杂志 2022年第1期42卷 16-23页

作者： W.joseph Herring Yuki Mukai Aobo Wang Jeannine Lutkiewicz John F.Lombard Li lin Molly Watkins David M.Broussard Manfred Blobner 美国默克集团临床研究部肯纳尔沃斯堡新泽西州07033美国奥克斯纳诊所基金会新奥尔良市洛杉矶70121美国慕尼黑工业大学医学院麻醉科与重症监护医学科慕尼黑80333德国乌尔姆大学医学院麻醉科与重症监护医学科乌尔姆89081德国

目的评价舒更葡糖钠用于ASA分级Ⅲ或Ⅳ级高风险手术患者术后拮抗神经肌肉阻滞的安全性。尤其着重评估了心脏不良事件以及其他预先指定的临床关注不良事件的影响。方法符合ASA分级Ⅲ或Ⅳ级标准的患者,根据ASA分级和神经肌肉阻滞剂(罗库... 详细信息

目的评价舒更葡糖钠用于ASA分级Ⅲ或Ⅳ级高风险手术患者术后拮抗神经肌肉阻滞的安全性。尤其着重评估了心脏不良事件以及其他预先指定的临床关注不良事件的影响。方法符合ASA分级Ⅲ或Ⅳ级标准的患者,根据ASA分级和神经肌肉阻滞剂(罗库溴铵或维库溴铵)进行随机分组,新斯的明/格隆溴铵组:中度神经肌肉阻滞;舒更葡糖钠2 mg/kg组,中度神经肌肉阻滞;舒更葡糖钠4 mg/kg组,深度神经肌肉阻滞;舒更葡糖钠16 mg/kg组,深度神经肌肉阻滞(仅罗库溴铵)。主要研究终点包括治疗期间窦性心动过缓、窦性心动过速和其他心律失常的发生情况。结果344例患者进入随机化分配,331例患者接受了治疗[61%为男性,BMI(28.5±5.3)kg/m^(2),年龄(69±11)岁]。舒更葡糖钠2 mg/kg组治疗期间窦性心动过缓发生率显著降低,舒更葡糖钠2 mg/kg组和4 mg/kg组治疗期间窦性心动过速发生率显著降低(P0.05)。结论ASA分级Ⅲ或Ⅳ级高风险手术患者使用舒更葡糖钠拮抗罗库溴铵或维库溴铵诱导的中度和深度神经肌肉阻滞的安全性良好。

关键词：舒更葡糖钠安全性 ASA分级Ⅲ或Ⅳ级

Bio-modulation of scaring Glaucoma Filtration Surgery using a novel application of coated magnesium

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Journal of Magnesium and Alloys 2021年第3期9卷 883-894页

作者： Xiangji Li Richard Filek Xiaomin Zhu Huanhuan Gao Liying Qiao Hong Liu lin Xie Yong Wang Fusheng Pan Cindy ML Hutnik Department of Ophthalmology Institute of Surgery ResearchDaping HospitalArmy Medical UniversityChongqing 400042China Department of Ophthalmology Western UniversityIvey Eye InstituteSt.Joseph's Health Care LondonLondonONCanada Department of Ophthalmology The Third Affiliated HospitalChongqing Medical UniversityChongqing 400016China Collage of Material Science&Engineering Chongqing UniversityChongqing 400045China National Engineering Research Center for Magnesium Alloys Chongqing UniversityChongqing 400045China

Filtration surgery still plays a mainstream role of treatment for *** scarring is the main cause of surgical *** study evaluated the biocompatibility and anti-proliferative properties of pure magnesium with three different coatings,which are hydroxyapatite(HA),dicalcium phosphate dihydrate(DCPD)and DCPD+stearic acid(SA),in a primary culture of human tenon's capsule fibroblasts(HTCFs)and in rabbit Glaucoma Filtration *** and glass were used as controls in vitro,and trabeculectomy was used as control in *** results show the number and shape of HTCFs seeded on different coatings showed less quantity and poor cell *** type of coated magnesium demonstrated significantly decreased metabolic activity of ***+SA showed higher cytotoxicity than the other *** inhibition of proliferation was observed with the DCPD+SA *** expression ofα-SMA was decreased in the cells when seeded on all of the coated magnesium *** vivo,no obvious adverse effects were observed after *** significant difference existed for any of the different samples regarding different ion concentrations in the aqueous *** inflammatory response in the titanium,DCPD and DCPD+SA treated eyes was more intense than in the trabeculectomy alone and HA *** Blot analysis showed that collagen-1 andα-SMA expression was significantly lower in the titanium,HA,DCPD and DCPD+SA groups compared with the *** coatings on magnesium were able to affect the corrosive properties,which in turn,influenced the morphology and function of *** coated magnesium may be considered a very promising biodegradable material for the next generation of glaucoma drainage devices.

关键词： Glaucoma Glaucoma drainage device Fibrosis Tenon's capsule fibroblasts Magnesium Coating

Outcomes of submucosal(T1b) esophageal adenocarcinomas removed by endoscopic mucosal resection

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World Journal of Gastrointestinal Endoscopy 2016年第20期8卷 763-769页

作者： Darren D Ballard Neel Choksi Jingmei lin Eun-Young Choi B joseph Elmunzer Henry Appelman Douglas K Rex Hala Fatima William Kessler John M DeWitt Division of Gastroenterology and Hepatology Medical College of Wisconsin Division of Gastroenterology University of Michigan Department of Pathology Indiana University Department of Pathology University of Michigan Division of Gastroenterology and Hepatology University of South Carolina Division of Gastroenterology and Hepatology Indiana University

AIM To investigate the outcomes and recurrences of p T1 b esophageal adenocarcinoma(EAC) following endoscopic mucosal resection(EMR) and associated *** Patients undergoing EMR with pathologically confirmed T1 b EAC at two academic referral centers were retrospectively *** were divided into 4 groups based on treatment following EMR:Endoscopic therapy alone(group A),endoscopic therapy with either chemotherapy,radiation or both(group B),surgicalresection(group C) or no further treatment/lost to follow-up(<12 mo)(group D).Pathology specimens were reviewed by a central ***-up data was obtained from the academic centers,primary care physicians and/or referring *** analysis was performed to identify factors predicting recurrence of *** Fifty-three patients with T1 b EAC underwent EMR,of which 32(60%) had adequate follow-up ≥ 12 mo(median 34 mo,range 12-103).There were 16 patients in group A,9 in group B,7 in group C and 21 in group *** follow-up in groups A to C was 34 mo(range 12-103).Recurrent EAC developed overall in 9 patients(28%) including 6(38%) in group A(median:21 mo,range:6-73),1(11%) in group B(median:30 mo,range:30-30) and 2(29%) in group C(median 21 mo,range:*** of 9 recurrences were local;of the 6 recurrences,5 were treated with endoscopy *** predictors of recurrence of EAC were *** Endoscopic therapy of T1 b EAC may be a reasonable strategy for a subset of patients including those either refusing or medically unfit for esophagectomy.

关键词： Esophageal cancer Submucosal T1b Endoscopic mucosal resection Chemotherapy Esophagectomy

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黄河中游生态水文模型及洪旱灾害风险评估

地理学报 2023年第7期78卷 1666-1676页

作者：汤秋鸿徐锡蒙贺莉彭守璋胡亚伟靳晓辉樊玉苗 GAFFNEY Paul Patrick joseph 祝欣荣邓浩鑫杨淋王志慧中国科学院地理科学与资源研究所中国科学院陆地水循环及地表过程重点实验室北京100101 西北农林科技大学水土保持研究所杨凌712100 黄河水利委员会黄河水利科学研究院郑州450003 北京大学城市与环境学院北京100871 清华大学水利水电工程系北京100084 中国科学院大学北京100049

黄河中游地区生态环境脆弱,易受到严重的洪旱灾害威胁。近年来,在大规模梯田建设和植被恢复工程等人类活动影响下,黄河中游水土流失治理水平稳步提高,发挥了消洪抗旱减灾的作用。但与此同时,黄河中游植被建设导致土壤干层加剧、产流产... 详细信息

黄河中游地区生态环境脆弱,易受到严重的洪旱灾害威胁。近年来,在大规模梯田建设和植被恢复工程等人类活动影响下,黄河中游水土流失治理水平稳步提高,发挥了消洪抗旱减灾的作用。但与此同时,黄河中游植被建设导致土壤干层加剧、产流产沙下降,增加了干旱风险;城市扩张也导致城市化地区孕灾环境剧变,暴雨洪水风险增加。目前针对变化环境下流域水文—泥沙—植被协同演变机理的认识还不够清楚,需要充分考虑工程扰动后流域水文—泥沙—植被重新建立新平衡的过程,科学评估大规模梯田建设、植被恢复工程和城市扩张等人类活动对洪旱灾害风险的影响,发展变化环境下黄河中游智慧化生态水文模型,预估大规模人类活动对洪旱灾害的长期后继影响,为黄河中游生态建设与水利工程协调发展有效防范化解洪旱灾害风险提供科技支撑。

关键词：人类活动气候变化生态水文模型洪旱灾害风险评估

The Mediating Effect of Corporate Governance on the Relationship Between Accounting Information and Stock Market Return of Listed Entities in Ghana

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Journal of Modern Accounting and Auditing 2022年第2期18卷 60-89页

作者： joseph Kwasi Agyemang Barjoyai Bin Bardai University of Eswatini ManziniEswatini Al-Madinah International University Kuala LumpurMalaysia

The study examined the mediating effect of corporate governance on the relationship between accounting information and stock market returns of listed entities on the Ghana Stock *** population of the study was forty(40)listed entities from 2007-2019 with 520 firm-year *** study applied a panel regression model that takes unobserved individual heterogeneity and distributional heterogeneity into *** addition,the study employed cross-section dependence test,Levin-lin-Chu,ImPesaran,Pesaran,Kao,and Larsson cointegration test,fully modified ordinary least square(FMOLS),and dynamic ordinary least square(DOLS).The results of unit root test showed that all the variables are integrated at first ***,the results of cointegration test revealed that accounting information variables were cointegrated in the long *** result of FMOLS and DOLS further revealed that all the accounting information variables with the exception of OCFPS and NTA have a direct insignificant relationship with the stock market *** study revealed that corporate governance which was proxied by board size also strengthens the relationship between TAT and stock market return and NTA and stock market return at 5%significant level under FMOLS and DOLS respectively.

关键词： accounting information corporate governance stock market return board size

Does Board Size Mediate the Relationship Between Accounting Information and Stock Market Return?Evidence From Listed Financial Firms in Ghana

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Journal of Modern Accounting and Auditing 2022年第4期18卷 143-164页

作者： joseph Kwasi Agyemang Barjoyai Bin Bardai University of Eswatini ManziniEswatini Al-Madinah International UniversityKuala LumpurMalaysia Al-Madinah International University Kuala LumpurMalaysia

The study examined the relationship between accounting information and stock market returns of listed financial entities on the Ghana Stock Exchange using board size as a mediating *** population of the study was 13 listed financial entities from 2007-2019 with 169 firm-year *** study applied a panel regression model that takes unobserved individual heterogeneity and distributional heterogeneity into *** addition,the study employed cross-section dependence test;Levin-lin-Chu(LLC),ImPesaran,Pesaran,Kao,and Larsson cointegration test;Fully Modified Ordinary Least Square(FMOLS)and Dynamic Ordinary Least Square(DOLS).The results of the unit root test showed that all the variables were integrated at first ***,the results of cointegration test revealed that accounting information variables were cointegrated in the long *** result of FMOLS and DOLS revealed that all the accounting information variables with the exception of operating cash flow per share(OCFPS)and net tangible assets(NTA)have a direct insignificant relationship with the stock market return of listed financial *** study revealed that board size strengthens the relationship between OCFPS and NTA,and stock market return at 5%significant level under FMOLS and DOLS of listed financial entities.

关键词： accounting information board size stock market return

A feasibility study of using biodegradable magnesium alloy in glaucoma drainage device

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International Journal of Ophthalmology(English edition) 2018年第1期11卷 135-142页

作者： Xiang-Ji Li lin Xie Fu-Sheng Pan Yong Wang Hong Liu Yu-Rong Tang Cindy-ML Hutnik Department of Ophthalmology University of Western Ontario Ivey Eye Institute St.Joseph's Hospital Department of Ophthalmology Institute of Surgery ResearchDaping Hospital Third Military Medical University Collage of Material Science & Engineering ChongqingUniversity National Engineering Research Center for Magnesium Alloys Chongqing University Department of Ophthalmology People's Hospital Zhongshan Branch

Technological advances in glaucoma have challenged the traditional treatment paradigm. Historically incisional surgery has been used in cases of advanced disease and/or uncontrolled intraocular pressures resistant to medical or laser interventions. More recently, perhaps due to advancements in imaging, surgery has been suggested to be beneficial earlier in the treatment paradigm. Despite these trends, surgical manipulation of the tissues and unpredictability of wound healing continue to result in surgical failure. Magnesium is an essential element for human body and plays a critically important role in maintaining the functional and structural integrity of several tissues, including the eye. Due to several of its advantageous properties such as non-toxicity, biodegradability, and high biological compatibility, magnesium alloy has attracted great attention as a novel biomaterial. Biodegradable cardiovascular stents made of magnesium alloy have already been introduced into clinical practice. The purpose of this review is to determine if bioabsorbable magnesium alloys can be utilized as a promising candidate for the development of a new generation of glaucoma surgical assistive devices.

关键词： glaucoma glaucoma drainage implant magnesium alloy anti-scarring