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Development of a multiprimer metabarcoding approach to understanding trophic interactions in agroecosystems

Insect Science 2022年第4期29卷 1195-1210页

作者： Ivan Batuecas Oscar Alomar Cristina Castañe josep Piñol Stéphane Boyer Lorena Gallardo-Montoya Nuria Agustí IRTA Sustainable Plant ProtectionCabrilsBarcelonaSpain Universitat Autònoma Barcelona Cerdanyola del VallèsSpain CREAF Cerdanyola del VallèsSpain Institut de Recherche sur la Biologie de l'Insecte(IRBI) Tours UniversityToursFrance

To understand trophic interactions and the precise ecological role of each predatory species,it is important to know which arthropod and plant resources are used by generalist predators in *** approaches,such as the use of high-throughput sequencing(HTS),play a key role in identifying these *** study develops a multiprimer metabarcoding approach for screening the most common trophic interactions of two predatory arthropods with contrasting morphologies,Rhagonycha fulva(Coleoptera:Cantharidae)and Anthocoris nemoralis(Hemiptera:Anthocoridae)collected from a peach *** reduce the time and cost of this metabarcoding approach,we first evaluated the effect of using two different predator-pools of different size(10 and 23 individuals of the same species).We also used our system to analyze the performance of one and two primer pairs in the same *** results show that the analysis of 23 individuals together with the use of two primer pairs in the same library optimize the HTS *** these best-performing conditions,we then analyzed the entire bodies of field-collected predators as well as the washing solutions used to clean the insect *** were able to identify both gut content(i.e.,diet)and external pollen load(i.e.,on the insects’bodies).This study also demonstrates the importance of washing predatory insects’bodies prior to HTS analysis when the target species have a considerable size(>10 mm)and hairy *** metabarcoding approach has significant potential for the study of trophic links in agriculture,revealing expected and unexpected trophic relationships.

关键词： high-throug hput sequencing metabarcoding molecular diet analysis multiprimer approach predatory arthropods trophic interactions

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Late Miocene/Early Pliocene vertebrate fauna from Mallorca(Balearic Islands,Western Mediterranean):an update

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Integrative Zoology 2014年第2期9卷 183-196页

作者： pere BOVER Juan ROFES Salvador BAILON Jordi AGUSTÍ Gloria CUENCA-BESCÓS Enric TORRES josep Antoni ALCOVER IMEDEA(CSIC-UIB) Mediterranean Institute for Advanced StudiesDepartment of Biodiversity and ConservationBalearic IslandsSpain Research Associate Division of Vertebrate Zoology/Mammalogy Department American Museum of Natural HistoryNew YorkNYUSA Department of Stratigraphy and Paleontology University of the Basque CountryLeioaSpain Grupo Aragosaurus-IUCA Area of PaleontologíaEarth Sciences DepartmentUniversity of ZaragozaZaragozaSpain UMR 7209-7194 of CNRS Department of Ecology and Biodiversity ManagementNational Museum of Natural HistoryParisFrance ICREA Institut Catalàde Paleoecologia Humana i EvolucióSocial(IPHES)Universitat Rovira i VirgiliTarragonaSpain

The vertebrate fossil record from the Balearic Islands(western Mediterranean)has improved considerably over the past decade,especially in Mallorca and *** Menorca,the Pliocene terrestrial fauna was updated by the discovery and description of the large-sized leporid Nuralagus,several reptiles and an *** Mal-lorca,paleontological exploration yielded 2 deposits with a Late Miocene/Early Pliocene chronology,Calóden Rafelino(CdR)and Na Burguesa-1(NB-1).So far,4 new mammalian taxa and 2 new reptiles have been iden-tified for the CdR deposit,whereas the faunal assemblage from the recently discovered deposit(Apr 2012)of NB-1 is currently composed of,at least,6 terrestrial mammals,8 reptiles and an *** faunal composi-tion and some primitive characteristics of the obtained taxa suggest that the chronology of this deposit is slight-ly earlier than the *** terrestrial vertebrates recorded in these 2 Mallorcan deposits are changing the view of the paleofaunal assemblage previously known for the Plio-Pleistocene of the *** charac-teristics displayed by some of the taxa suggest that these faunas would be at the beginning of an isolated *** this paper we present a preliminary report on the fossils recovered from the NB-1 deposit,as well as some unpublished data from CdR,and we analyze the whole fauna from both Mallorcan deposits,focusing on taxonomical and paleobiogeographical aspects.

关键词： Early Pliocene Late Miocene Mallorca new fauna paleobiogeography

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New real-time-PCR method to identify single point mutations in hepatitis C virus

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World Journal of Gastroenterology 2016年第43期22卷 9604-9612页

作者： Qian Chen Irene Belmonte Maria Buti Leonardo Nieto Damir Garcia-Cehic josep Gregori Celia perales Laura Ordeig Meritxell Llorens Maria Eugenia Soria Rafael Esteban Juan Ignacio Esteban Francisco Rodriguez-Frias josep Quer Liver Unit Internal Medicine Lab. Malalties Hepàtiques Vall d’Hebron Institut Recerca-Hospital Universitari Vall d’ Hebron08035 Barcelona Spain Centro de Investigación Biomédica en Red Enfermedades Hepáticas y Digestivas Instituto de Salud Carlos Ⅲ28029 madrid Spain Liver Pathology Unit Departments of Biochemistry and Microbiology Hospital Universitari Vall d’Hebron Autonomous University of Barcelona 08035 Bareelona Spain Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas del Instituto de Salud Carlos Ⅲ 28029 Madrid Spain Universitat Autònoma de Barcelona 08193 Bareelona Spain

AIM To develop a fast, low-cost diagnostic strategy to identify single point mutations in highly variable genomes such as hepatitis C virus(HCV).METHODS In patients with HCV infection, resistance-associated amino acid substitutions within the viral quasispecies prior to therapy can confer decreased susceptibility to direct-acting antiviral agents and lead to treatment failure and virological relapse. One such naturally occurring mutation is the Q80 K substitution in the HCV-NS3 protease gene, which confers resistance to PI inhibitors, particularly simeprevir. Low-cost, highly sensitive techniques enabling routine detection of these single point mutations would be useful to identify patients at a risk of treatment failure. Light Cycler methods, based on real-time PCR with sequencespecific probe hybridization, have been implemented in most diagnostic laboratories. However, this technique cannot identify single point mutations in highly variable genetic environments, such as the HCV genome. To circumvent this problem, we developed a new method to homogenize all nucleotides present in a region except the point mutation of interest. RESULTS Using nucleotide-specific probes Q, K, and R substitutions at position 80 were clearly identified at a sensitivity of 10%(mutations present at a frequency of at least 10% were detected). The technique was successfully applied to identify the Q80 K substitution in 240 HCV G1 serum samples, with performance comparable to that of direct Sanger sequencing, the current standard procedure for this purpose. The new method was then validated in a Catalonian population of 202 HCV G1-infected individuals. Q80 K was detected in 14.6% of G1 a patients and 0% of G1 b in our setting. CONCLUSION A fast, low-cost diagnostic strategy based on real-time PCR and fluorescence resonance energy transfer probe melting curve analysis has been successfully developed to identify single point mutations in highly variable genomes such as hepatitis C virus. This

关键词： Hepatitis C virus Resistance-associated amino acid substitutions Low-cost test Single-point mutations Q80K

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The Last Pterosaurs: First Record from the Uppermost Maastrichtian of the Tremp Syncline (Northern Spain)

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Acta Geologica Sinica(English Edition) 2013年第5期87卷 1198-1227页

作者： DALLA VECCHIA Fabio M. RIERA Violeta OMS josep Oriol DINARS-TURELL Jaume GAETE Rodrigo GALOBART Angel Institut Català de Paleontologia "M. Crusafont" (ICP) Grup de Recerca del Mesozoic Escola Industrial 23E-08201 SabadellSpain Universitat Autònoma de Barcelona Departament de GeologiaCerdanyola del VallèsBarcelonaE-08193Spain Istituto Nazionale di Geofisica e Vulcanologia Laboratorio di PaleomagnetismoVia di Vigna Murata 605I-00143 RomaItaly Museu de la Conca Dellà C/del Museu 4E-25650 IsonaSpain

Pterosaur bones are for the first time reported from the Tremp Syncline （Spain） and the southern Pyrenees. They come from the Torrebilles-2 site （Isona, Lleida Province） in the upper Maastrichtian part of the Tremp Formation, which falls within the C29r magnetochron. Fragmentary long bones are referred to the Pterosauria based on their cortical thinness, micro-architecture, and histological features. They belong to very large to possibly giant individuals. The most complete specimen is possibly a partial right femur. The review of the Maastrichtian pterosaur record shows that only a few specimens can be referred to the C29r （which contains the K/Pg boundary）. The identification of fragmentary remains could improve our knowledge about the abundance and distribution of these flying sauropsids just before the end-Cretaceous mass extinction.

关键词： Pterosauria bone morphology histology latest Maastrichtian European Archipelago,Ibero-Armorican Island Tremp Syncline.

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Diversification of SUMO-Activating Enzyme in Arabidopsis： Implications in SUMO Conjugation

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Molecular Plant 2013年第5期6卷 1646-1660页

作者： Laura Castano-Miquel josep Segui Silvia Manrique Ines Teixeira Lorenzo Carretero-Paulet Felix Atencio L. Maria Lois Center for Research in Agricultural Genomics CRAG (CSIC-IRTA-UAB-UB) Edifici CRAG-Campus UAB Bellaterra (Cerdanyola del Valles) 08193 Barcelona Spain Department of Biological Sciences SUNY-University at Buffalo North Campus 109 Cooke Hall Buffalo NY 14260 USA

Sumoylation is an essential posttranslational modification that participates in many biological processes including stress responses. However, little is known about the mechanisms that control Small Ubiquitin-like MOdifier （SUMO） conjugation in vivo. We have evaluated the regulatory role of the heterodimeric E1 activating enzyme, which catalyzes the first step in SUMO conjugation. We have established that the E1 large SAE2 and small SAE1 subunits are encoded by one and three genes, respectively, in the Arabidopsis genome. The three paralogs genes SAEla, SAElbl, and SAElb2 are the result of two independent duplication events. Since SAElbl and SAElb2 correspond to two identical cop- ies, only two E1 small subunit isoforms are present in vivo： SAEla and SAElb. The E1 heterodimer nuclear localization is modulated by the C-terminal tail of the SAE2 subunit. In vitro, SUMO conjugation rate is dependent on the SAE1 isoform contained in the E1 holoenzyme and our results suggest that downstream steps to SUMO-E1 thioester bond formation are affected. In vivo, SAEla isoform deletion in T-DNA insertion mutant plants conferred sumoylation defects upon abi- otic stress, consistent with a sumoylation defective phenotype. Our results support previous data pointing to a regula- tory role of the E1 activating enzyme during SUMO conjugation and provide a novel mechanism to control sumoylation in vivo by diversification of the E1 small subunit.

关键词： E1 activating enzyme conjugation rate subcellular localization regulation abiotic stress gene duplication.

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考虑电池储能系统荷电状态的有功功率协调控制

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电力系统自动化 2015年第22期39卷 30-36页

作者：唐芬姜久春吴丹周啸 josep M.GUERRERO 北京交通大学国家能源主动配电网技术研发中心北京市100044 北京交通大学北京电动车辆协同创新中心北京市100044 丹麦奥尔堡大学能源技术系

微电网孤岛运行时,若供需存在较长时间的不平衡,传统的无互联信号线有功功率协调控制可能导致电池储能系统(BESS)荷电状态(SOC)超出安全运行范围。针对该问题,提出了一种考虑SOC的多BESS与可再生能源(RES)间的分布式有功功率协调控制策... 详细信息

微电网孤岛运行时,若供需存在较长时间的不平衡,传统的无互联信号线有功功率协调控制可能导致电池储能系统(BESS)荷电状态(SOC)超出安全运行范围。针对该问题,提出了一种考虑SOC的多BESS与可再生能源(RES)间的分布式有功功率协调控制策略。该方法通过基于瞬时功率的变斜率下垂控制、基于SOC的恒功率比例—积分(PI)下垂控制以及基于母线频率的功率下垂控制的集成来实现有功功率的无互联信号线自治协调控制。所提控制方法不仅可简单植入BESS和RES的控制系统中而无需修改其内环控制结构,而且能使SOC处于安全范围的同时最大化RES利用。实时仿真结果表明了所提策略的有效性。

关键词：微电网(微网) 自治功率分配功率下垂控制协调控制

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Tailoring RGD local surface density at the nanoscale toward adult stem cell chondrogenic commitment

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Nano Research 2017年第6期10卷 1959-1971页

作者： Anna Lagunas Iro Tsintzou Yolanda Vida Daniel Collado Ezequiel perez-Inestrosa Cristina Rodriguez pereira Joana Magalhaes Jose A. Andrades josep Samitier Networking Biomedical Research Center (CIBER) C/Monforte de Lemos 3-5 Pabellon 11 Planta 0 Madrid 28029 Spain Institute for Bioengineering of Catalonia (IBEC) Baldiri-Reixac 10-12 Barcelona 08028 Spain Instituto de Investigacion Biomedica de Malaga (IBIMA) Department of Organic Chemistry Universidad de Malaga (UMA) Malaga 29071 Spain Andalusian Centre for Nanomedicine and Biotechnology-BlONAND Parque Tecnoldgico de Andalucia Mdlaga 29590 Spain Unidad de Bioingenieria Tisulary Terapia Celular (GBTTC-CHUAC) Grupo de Reumatologia Instituto de Investigacion Biomedica de A Coru6a (INIBIC) Complexo Hospitalario Universitario de A Coruf~a (CHUAC) Sergas Universidade da Coruna (UDC) As Xubias 84 Coruna 15006 A Spain Cell Biology Genetics and Physiology Department University de Malaga (UMA) Campus Teatinos Malaga 29071 Spain Department of Engineering Electronics University of Barcelona (UB) Marti i Franques 1-11 Barcelona 08028 Spain

Arginine-glycine-aspartic acid （RGD） dendrimer-based nanopatterns on poly（L- lactic acid） were used as bioactive substrates to evaluate the impact of the RGD local surface density on the chondrogenic induction of adult human mesenchymal stem cells. During chondrogenic commitment, active extracellular matrix （ECM） remodeling takes place, playing an instructive role in the differentiation process. Although three-dimensional environments such as pellet or micromass cultures are commonly used for in vitro chondrogenic differentiation, these cultures are rather limited with respect to their ability to interrogate cells in celI-ECM interactions. In the present study, the nanopatterns of the tunable RGD surface density were obtained as a function of the initial dendrimer concentration. The local RGD surface density was quantified through probability contour plots for the minimum interparticle distance, constructed from the corresponding atomic force microscopy images, and correlated with the cell adhesion and differentiation response. The results revealed that the local RGD surface density at the nanoscale acts as a regulator of chondrogenic commitment, and that intermediate adhesiveness of cells to the substrates favors mesenchymal cell condensation and early chondrogenic differentiation.

关键词： dendrimer arginine-glycine-asparticacid (RGD) nanopattem human mesenchymalstem cells (hMSCs) chondrogenesis

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ScY@C_(3v)(8)-C_(82):Metal-Metalσ^(2)Bond in Mixed Rare-Earth Di-metallofullerenes

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Chinese Journal of Chemistry 2023年第15期41卷 1809-1814页

作者： Lihao Zheng Yannick Roselló Yingjing Yan Yang-Rong Yao Xiaolin Fan josep M.Poblet Antonio Rodríguez-Fortea Ning Chen College of Chemistry Chemical Engineering and Materials Scienceand State Key Laboratory of Radiation Medicine and ProtectionSoochow UniversitySuzhouJiangsu 215123China Departament de Quimica Fisica i Inorganica Universitat Rovira i VirgiliMarcelli Domingo 143007 TarragonaSpain Department of Materials Science and Engineering CAS Key Laboratory of Materials for Energy ConversionAnhui Laboratory of Advanced Photon Science and TechnologyUniversity of Science and Technology of ChinaHefeiAnhui 230026China

Comprehensive Summary The experimental investigation of rare-earth metal-metal bonds remains a challenge in the study of chemical ***,we report the synthesis and characterization of a novel heteronuclear di-metallofullerene,ScY@C_(3v)(8)-C_(82),which contains a mixed rare-earth metal-metal ***@C_(3v)(8)-C_(82)was successfully synthesized by arc-discharging method and characterized by mass spectrometry,UV-vis-NIR spectroscopy and single-crystal X-ray diffraction crystallography,which unambiguously determined its molecular *** calculations were also performed to study the optimized positions of Sc-Y metallic dimer and the electronic *** combined experimental and theoretical results confirmed that both Sc and Y atoms transfer two electrons to the C_(3v)(8)-C_(82)cage,i.e.,(ScY)4+@(C_(3v)(8)-C_(82))4-.In particular,a covalent Sc-Yσ2 bond,which has never been reported before,is proven to be formed inside C_(3v)(8)-C_(82)fullerene *** work presents a novel di-metallofullerene containing mixed rare-earth metal-metal bond and expands the understanding of metal-metal bonding of rare earth elements.

关键词： Fullerenes Endohedral fullerenes Rare earths Densityfunctional calculations Metal-metal bond

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适用于交直流混合微电网的直流分层控制系统

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电工技术学报 2013年第4期28卷 35-42页

作者：陆晓楠孙凯 josep Guerrero 黄立培清华大学电力系统及大型发电设备安全控制和仿真国家重点实验室北京100084 丹麦奥尔堡大学能源技术系

为了改进交直流混合微电网中直流侧的母线电压性能,本文提出了直流分层控制系统,以在各接口变换器之间合理分配直流负荷,同时补偿下垂控制带来的直流母线电压跌落。所介绍的分层控制系统包括两层:第一层控制直流母线电压和交流电流,同... 详细信息

为了改进交直流混合微电网中直流侧的母线电压性能,本文提出了直流分层控制系统,以在各接口变换器之间合理分配直流负荷,同时补偿下垂控制带来的直流母线电压跌落。所介绍的分层控制系统包括两层:第一层控制直流母线电压和交流电流,同时通过下垂控制实现直流侧的负荷功率分配;第二层补偿下垂控制带来的电压跌落,进一步提升母线电压质量。在第二层控制中,采用了具有低带宽通信特点的公共控制器,满足了微电网分布式结构的需要。本文给出了各控制层的具体设计方法。同时,对第二层控制的低带宽通信特性进行了具体的理论分析。仿真和实验结果表明了理论分析的正确性。

关键词：混合微电网并联接口变换器分层控制下重控制

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Super-Resolution Displacement Spectroscopic Sensing over a Surface“Rainbow”

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Engineering 2022年第10期17卷 75-81页

作者： Lyu Zhou Nan Zhang Chang Chieh Hsu Matthew Singer Xie Zeng Yizheng Li Haomin Song josep Jornet Yun Wu Qiaoqiang Gan Department of Electrical Engineering The State University of New York at BuffaloBuffaloNY 14260USA Department of Biomedical Engineering The State University of New York at BuffaloBuffaloNY 14260USA Department of Electrical and Computer Engineering Northeastern UniversityBostonMA 02115USA Material Science Engineering Program King Abdullah University of Science and TechnologyThuwal 23955-6900Saudi Arabia

Subwavelength manipulation of light waves with high precision can enable new and exciting applications in spectroscopy,sensing,and medical *** these applications,miniaturized spectrometers are desirable to enable the on-chip analysis of spectral *** particular,for imaging-based spectroscopic sensing mechanisms,the key challenge is to determine the spatial-shift information accurately(i.e.,the spatial displacement introduced by wavelength shift or biological or chemical surface binding),which is similar to the challenge presented by super-resolution ***,we report a unique"rainbow"trapping metasurface for on-chip spectrometers and *** with super-resolution image processing,the low-setting 4×optical microscope system resolves a displacement of the resonant position within 35 nm on the plasmonic rainbow trapping metasurface with a tiny area as small as0.002 *** unique feature of the spatial manipulation of efficiently coupled rainbow plasmonic resonances reveals a new platform for miniaturized on-chip spectroscopic analysis with a spectral resolution of 0.032 nm in wavelength *** this low-setting 4×microscope imaging system,we demonstrate a biosensing resolution of 1.92×109exosomes per milliliter for A549-derived exosomes and distinguish between patient samples and healthy controls using exosomal epidermal growth factor receptor(EGFR)expression values,thereby demonstrating a new on-chip sensing system for personalized accurate bio/chemical sensing applications.

关键词： Rainbow trapping Metasurface Surface plasmon polaritons Super-resolution displacement On-chip biosensing

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