检索结果-南通市图书馆

Complex Regulation of Prolyl-4-Hydroxylases Impacts Root Hair Expansion

Molecular Plant 2015年第5期8卷 734-746页

作者： Silvia M. Velasauez Martiniano M. Ricardi Christian Peter Poulsen Ai Oikawa Adiphol Dilokpimol Adnan Halim Silvina Mangano Silvina Paola Denita Juarez Eliana Marzol Juan D. Salgado Salter Javier Gloazzo Dorosz Cecilia Borassi Svenning Rune Moller Rafael Buono Yukiko Ohsawa Ken Matsuoka Marisa S. Otegui Henrik V. Scheller Naomi Geshi Bent Larsen Petersen Norberto D. lusem jose M. Estevez Instituto de Fisiologfa Biologia Molecular y Neurociencias (IFIBYNE-CONICET) Facultad de Ciencias Exactas y Naturales Universidad de Buenos AiresBuenos Aires C1428EGA Argentina VKR Research Centre Department of Plant and Environmental Sciences University of Copenhagen DK-1871 Frededksberg C Denmark Joint BioEnergy Institute Feedstocks Division Lawrence Berkeley National Laboratory 5885 Hollis Street Emeryville CA 94608 USA Copenhagen Center for Glycomios Department of Cellular and Molecular Faculty of Health and Medical Sciences University of CopenhagenDK-2200 Copenhagen N Denmark Department of Botany University of Wisconsin Madison WI 53706 USA RIKEN Plant Science Center Tsurumi-ku Yokohama 230-0045 Japan Laboratory of Plant Nutrition Faculty of Agriculture Kyushu University Hakozaki 6-10-1 Higashi-ku Fukuoka 812-8581 Japan Departamento de Fisiologia Biologia Molecular y Celular Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires Buenos Aires C1428EGAArgentina These authors contributed equally to the article. Present address: Fungal Physiology CBS-KNAW Fungal Biodiversity Center Uppsalalaan 8 Utrecht 3584 CT The Netherlands Present address: The Danish Stem Cell Center The Panum Institute Blegdamsvej 3B DK-2200 Copenhagen Denmark

Root hairs are single cells that develop by tip growth, a process shared with pollen tubes, axons, and fungal hyphae. However, structural plant cell walls impose constraints to accomplish tip growth. In addition to polysaccharides, plant cell walls are composed of hydroxyproline-rich glycoproteins （HRGPs）, which include several groups of O-glycoproteins, including extensins （EXTs）. Proline hydroxylation, an early post-translational modification （PTM） of HRGPs catalyzed by prolyl 4-hydroxylases （P4Hs）, defines their subsequent O-glycosylation sites. In this work, our genetic analyses prove that P4H5, and to a lesser extent P4H2 and P4H13, are pivotal for root hair tip growth. Second, we demonstrate that P4H5 has in vitro preferred specificity for EXT substrates rather than for other HRGPs. Third, by P4H promoter and protein swapping approaches, we show that P4H2 and P4H13 have interchangeable functions but cannot replace P4H5. These three P4Hs are shown to be targeted to the secretory pathway, where P4H5 forms dimers with P4H2 and P4H13. Finally, we explore the impact of deficient proline hydroxylation on the cell wall architec- ture. Taken together, our results support a model in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assembly and hence root hair elongation in Arabidopsis thaliana.

关键词： enzymology cell expansion cell walls protein targeting proline hydroxylation root hairs

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

In <i>Vitro</i>Effects of the Phytocomplex TrichoTech<sup>TM</sup>on Human Fibroblasts: Proliferative Potential and Effects on Gene Expression of FGF-7 and FGF-10

引用

Journal of Cosmetics, Dermatological Sciences and Applications 2017年第1期7卷 1-13页

作者： Fernando Amaral Maira Jardim Valeria Maria de Souza Antunes luis Felipe Gomes Michelin Bárbara Anaís Rodrigues dos Santos Christiano Marcelo Vaz Barbosa Daniel Gonsales Spindola Claudia Bincoletto Carlos Rocha Oliveira Instituto de Osmologia e óleos Essenciais Minas Gerais Brazil Grupo de Fitocomplexos e Sinalizaç ã o Celular Escola de Ciência da Saúde Universidade Anhembi Morumbi S&atilde o Paulo Brazil Centro Interdisciplinar de Investigaç ã o Bioquímica Universidade de Mogi das Cruzes S&atilde o Paulo Brazil Centro Interdisciplinar de Investigaç ã o Bioquímica Universidade de Mogi das Cruzes S&atilde o Paulo Brazil Departamento de Farmacologia Universidade Federal de S&atilde o Paulo S&atilde o Paulo Brazil

The human hair follicle, a mini-organ formed with neuroectodermal-meso-dermal interaction, is a complex structure, in the active steady state (anagen) the dermal papilla can be considered as a ball of extracellular matrix, surrounding specialized fibroblasts. The cross-talk of dermal papilla with neighbouring matrix cells results in the maintenance of hair fibre production. This study aimed to investigate the proliferative potential of the compound TrichotechTM, a phytocomplex obtained from a mixture of essential oils, on cultured human fibroblasts and its ability to modulate the gene expression of FGF-7 and FGF-10. TrichotechTM was shown to enhance fibroblasts proliferation in concentrations of 0.5% to 2.0%, and also increase the percentage of cells in the S/G2/M phases of the cell cycle. TrichotechTM at both 1.0% and 2.0% induced a statistically significant effect on wound healing assay compared to the untreated control. We examined the interaction between cell survival (PI3K/Akt) and mitogenic (Ras/MAPK) signal transduction pathways after TrichotechTM treatment (1.0% and 2.0%) on the fibroblast cell line. TrichotechTM caused phosphorylation of ERK1/2, as well as greater phosphorylation of MEK in comparison with both the untreated control and ERK1/2. PI3K and AKT, however, were not shown to be significantly more phosphorylated following TrichotechTM exposure. To verify the relative expression of mRNA for FGF-7 and FGF-10 genes, a real-time polymerase chain reaction (qPCR) protocol was used. Results show the increase in mRNA expression by fibroblasts after treatment with TrichotechTM. In both concentrations tested, TrichotechTM was found to increase the expression of FGF-7 and FGF-10. Sirius red staining allows for rapid assessment of collagen content, it showed a significant increase in collagen content in treated fibroblasts. Further investigation concerning TrichotechTM could be helpful towards the development of new bioactive phytocomplexes for dermatological a

关键词： TrichoTechTM Fibroblasts Proliferation FGF-7 FGF-10

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

My Life in Beijing

引用

China's Foreign Trade 2011年第6期 84-84页

作者： jose Alberto Limas G. Second Secretary of Embassy Of Mexico in China Responsible of Commercial Affairs

I came to live in Beijing in February *** is the first city I live abroad Mexico and after five year, I feel it like a second home.I enjoy when I travel to another city in China, but after a few days,I miss Beijing. I am from Mexico City,which is very similar to *** are really big 20 million people or *** are old cities,deeply proud to be the capitals and cultural centers of countries with a long and incredibly rich *** and Mexico City are perfect combinations of the old and the new,tradition and modernity, convenience and *** are organized around a magnificent square surrounded by the symbols of tradition, history and political *** City was the first city from a developing coun-

关键词：北京生活墨西哥城市

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

EXPO SHANGHAI,Really Impressive and Unforgettable for Me!

引用

China's Foreign Trade 2011年第5期 91-91页

作者： jose Alberto Limas G. Second Secretary of Embassy of Mexico in China Responsible of Commercial Affairs

Time *** the moment last year,I was expecting the beginning of the World Expo *** one year has passed since the curtain of Expo Shanghai *** my stay in China,one of the most impressive things for me,I should say,is the Expo Shanghai. There were just so many things which impressed *** Expo site was impressive for its size,all the facilities built,the magnificent and creative pavilions,the army of people and volunteers assisting the thousands of visitors, but perhaps the most impressive thing was the will of China to reach the world hosting the Expo and the relevant will of the rest of the world

关键词：对外贸易进出口贸易 CPI 中国

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Optimization of Aspergillus ***4 Tannase Production under Submerged Fermentation

引用

Advances in Microbiology 2014年第3期4卷 143-150页

作者： Alessandra Goncalves de Melo Rayssa Cristina Faria Pedroso luis Henrique Souza Guimaraes José Guilherme Lembi Ferreira Alves Eustáquio Souza Dias Mário Lúcio Vilela de Resende Patrícia Gomes Cardoso Department of Biology Sector of MicrobiologyFederal University of LavrasLavrasBrazil Department of Biology University of Sao PauloRibeirao PretoBrazil Department of Food Science Federal University of LavrasLavrasBrazil Department of Phytopathology Federal University of LavrasLavrasBrazil

Tannase is a hydrolytic enzyme that is involved in the biodegradation of tannins and it has biotechnological potential in the pharmaceutical, chemical, food and beverage industries. Microorganisms, especially filamentous fungi, are important tannase producers. The aims of this work were to find a potential tannase producer and to improve the cultivation conditions. Three Aspergillus species (A. japonicus 246A, A. tamarii 3 and Aspergillus sp. GM4) were investigated in different culture media (Adams, Czapeck, Khanna, M5 and Vogel) and inducers (1% and 2% tannic acid;1% green tea;1% methyl gallate;1% gallic acid). Aspergillus sp. GM4 and Adams medium were selected. The tannase production by Aspergillus sp. GM4 in Adams medium was induced in the presence of 2% (w/v) tannic acid and gallic acid as carbon sources, while green tea was not able to induce tannase production. The Plackett-Burman screening design was performed with the variables MgSO4, KH2PO4, yeast extract, tannic acid, agitation rate and salt solution. The variables MgSO4 and agitation rate were selected for the optimization of tannase production using a Central Composite Rotatable Design. Under optimized conditions, a 2.66-fold increase in the enzyme production was observed with small modifications in the medium composition.

关键词： Aspergillus sp. Response Surface Methodology Submerged Fermentation Tannase Tannic Acid

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Greenlight Photoselective Vaporisation of the Prostate in 133 High Surgical Risk Patients: A 5-Year Outcome Study

引用

Open Journal of Urology 2013年第2期3卷 90-95页

作者： Ignacio Gómez García Marta Romero Molina Emilio Rubio Hidalgo luis álvarez Buitrago Natanael García Betancourt Larissa Lara Pablo Gutierrez Antonio Sampietro Crespo Fernando álvarez Férnandez Antonio Gómez Rodríguez LMR Group Madrid Spain Urology Service Hospital Río Ortega Valladolid Spain Urology Service Hospital Virgen de la Salud Toledo Spain

Greenlight photoselective vaporisation of the prostate (GPVP) is progressively becoming an established treatment in patients with LUTS because it is a minimally invasive technique that achieves efficient haemostasis, making it the ideal technique for patients at high surgical risk. Material and Methods: To study of 133 patients with an ASA surgical risk score of 3 or 4, undergoing GPVP, with an analysis of perioperative outcome, IPSS, Qmax, IIEF-5 and complications during a five-year follow-up. Results: At 5 years the mean annual improvement in IPSS was stable, and at 5 years there was a 15.2 point improvement versus the preoperative score (p 0.05). The Qmax showed an improvement of 14.9 ml/sec and was maintained at five years after surgery (p 0.05). No patients were transfused or suffered urinary incontinence. 2.25% suffered major complications and there were no deaths. 3.1% of patients suffered de novo urgency. In the 5-year follow-up, five patients had to be reoperated. The quality of sexual health assessed by IIEF-5 before the procedure was scored at 14 points;the 5-year follow-up covering the preoperative period and all revisions did not show any worsening in the IIEF-5 score (p > 0.05). Conclusions: Due to its physical characteristics, in our opinion GPVP is now the treatment of choice in patients at high surgical risk. In our series, the risk of major/minor complications and transfusions was much lower than the same risks in conventional techniques. The objective results (Qmax and quality of life questionnaire) are equivalent to conventional techniques and persist over a 5-year follow-up.

关键词： Greenlight Vaporisation PVP High Risk ASA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A WW Domain-Containing Protein Forms Immune Nuclear Bodies against Begomoviruses

引用

Molecular Plant 2018年第12期11卷 1449-1465页

作者： lara P. Calil lana P.S. Quadros Thais C. Araujo Christiane E.M. Duarte Bianca C. Gouveia-Mageste jose Cleydson F. Silva Otavio J.B. Brustolini Ruan M. Teixeira Caue N. Oliveira Rafael W.M.M. Milagres Gilberto S. Martins Joanne Chory Pedro A.B. Reis Joao Paulo B. Machado Elizabeth P.B. Fontes Departament of Biochemistry and Molecular Biology Universidade Federal de Vigosa Vigosa Minas Gerais 36570-000 Brazil Nati onal In stitute of Science and Tech no logy in Plant-Pest Interactions Bioagro Universidade Federal de Vicosa Vinosa Minas Gerais 36570-000 Brazil Departament of Genetics Universidade Federal do Rio de Janeiro Rio de Janeiro Rio de Janeiro Brazil Howard Hughes Medical Institute and Plant Biology Laboratory The Salk Institute of Biological Studies La Jolla CA 92037 USA Agronomy Institute Universidade Federal de Vigosa Campus Florestal Florestal Minas Gerais 35690-000 Brazil These authors contributed equally to this article

The bipartite begomoviruses (Geminiviridae family), which are DNA viruses that replicate in the nucleus of infected cells, encode the nuclear shuttle protein (NSP) to facilitate the translocation of viral DNA from the nucleus to the cytoplasm via nuclear pores. This intracellular trafficking of NSP-DNA complexes is accessorized by the NSP-interacting guanosine triphosphatase (NIG) at the cytosolic side. Here, we report the nuclear redistribution of NIG by AtWWPI, a WW domain-containing protein that forms immune nuclear bodies (NBs) against begomoviruses. We demonstrated that AtWWPI relocates NIG from the cytoplasm to the nucleus where it is confined to AtWWP1-NBs, suggesting that the NIG-AtWWP1 interaction may interfere with the NIG pro-viral function associated with its cytosolic localization. Consistent with this assumpti on joss AtWWPI functi on cuased plants more susceptible to begomovirus infection, whereas overexpression of AWWP1 enhaneed plant resistance to begomovirus. Furthermore, we found that a mutant versi on of AtWWPI defective for NB formatio n was noIonger capable of interacti ng with and relocating NIG to the nucleus and lost its immune function against begomovirus. The antiviral function of AtWWP1-NBs, however, could be antagonized by viral infection that induced either the disruption or a decrease in the number of AtWWP1-NBs. Collectively, these results led us to propose that AtWWPI organizes nuclear structures into nuclear foci, which provide intrinsic immunity against begomovirus infection.

关键词： nuclear bodies antiviral immunity begomovirus NIG AtWWP1

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Intraoperative laparoscopic complications for urological cancer procedures

引用

World Journal of Clinical Cases 2015年第5期3卷 450-456页

作者： Sergio Fernández-Pello Montes Ivan Gonzalez Rodríguez Rodrigo Gil Ugarteburu luis Rodríguez Villamil Begoa Diaz Mendez Patricio Suarez Gil Javier Mosquera Madera Urology Department Cabueñes Hospital33203 GijónAsturiasSpain

AIM: To structure the rate of intraoperative complications that requires an intraoperative or perioperative resolution. METHODS: We perform a literature review of Medline database. The research was focused on intraoperative laparoscopic procedures inside the field of urological oncology. General rate of perioperative complications in laparoscopic urologic surgery is described to be around 12.4%. Most of the manuscripts published do not make differences between pure intraoperative, intraoperative with postoperative consequences and postoperative complications. RESULTS: We expose a narrative statement of complications, possible solutions and possible preventions for most frequent retroperitoneal and pelvic laparoscopic surgery. We expose the results with the following order: retroperitoneal laparoscopic surgery(radical nephrectomy, partial nephrectomy, nephroureterectomy and adrenalectomy) and pelvic laparoscopic surgery(radical prostatectomy and radical cystectomy).CONCLUSION: Intraoperative complications vary from different series. More scheduled reports should be done in order to better understand the real rates of complications.

关键词： Intraoperative complications Laparoscopy Surgical complication Urology Cancer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Boron nitride nanoresonators for phonon-enhanced molecular vibrational spectroscopy at the strong coupling limit

引用

Light(Science & Applications) 2017年第1期6卷 55-62页

作者： Marta Autore Peining Li Irene Dolado Francisco J Alfaro-Mozaz Ruben Esteban Ainhoa Atxabal Fèlix Casanova luis E Hueso Pablo Alonso-González Javier Aizpurua Alexey Y Nikitin Saül Vélez Rainer Hillenbrand CIC nanoGUNE Donostia-San Sebastián 20018Spain Donostia International Physics Center(DIPC) Donostia-San Sebastián 20018Spain IKERBASQUE Basque Foundation for ScienceBilbao 48013Spain Departamento de Física Universidad de OviedoOviedo 33007Spain Centro de Física de Materiales(MPC CSIC-UPV/EHU)Donostia-San Sebastián 20018Spain Current address:Department of Materials ETH ZürichZürich 8093Switzerland CIC nanoGUNE and UPV/EHU Donostia-San Sebastián 20018Spain

Enhanced light-matter interactions are the basis of surface-enhanced infrared absorption(SEIRA)spectroscopy,and conventionally rely on plasmonic materials and their capability to focus light to nanoscale spot *** polariton nanoresonators made of polar crystals could represent an interesting alternative,since they exhibit large quality factors,which go far beyond those of their plasmonic *** recent emergence of van der Waals crystals enables the fabrication of highquality nanophotonic resonators based on phonon polaritons,as reported for the prototypical infrared-phononic material hexagonal boron nitride(h-BN).In this work we use,for the first time,phonon-polariton-resonant h-BN ribbons for SEIRA spectroscopy of small amounts of organic molecules in Fourier transform infrared ***,the interaction between phonon polaritons and molecular vibrations reaches experimentally the onset of the strong coupling regime,while numerical simulations predict that vibrational strong coupling can be fully *** polariton nanoresonators thus could become a viable platform for sensing,local control of chemical reactivity and infrared quantum cavity optics experiments.

关键词： boron nitride phonon polaritons strong coupling surface-enhanced infrared absorption spectroscopy SEIRA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

杜撰集

引用

作者： (阿根廷)豪尔赫·路易斯·博尔赫斯(Jorge luis Borges)

来源：南通市图书馆图书评论

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：