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Alterations in fibrinolytic system proteins PAI-1,MMP-3,MMP-8,TIMP-1 and TIMP-2 in post-cholecystectomy bile duct injury

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Journal of Biomedical Science and Engineering 2013年第8期6卷 58-67页

作者： jose Manuel Hermosillo-Sandoval luis Miguel Román-Pintos Adolfo Daniel Rodriguez-Carrizález Ernesto Germán Cardona-Munoz Fermin Paul Pacheco-Moisés Genaro Gabriel Ortiz Alejandra Guillermina Miranda-diaz Department of Surgery Specialties Hospital of the National Occidental Medical CentreSocial Security Institute of Mexico(Departamento de CirugíaHospital de Especialidades del Centro Médico Nacional de OccidenteInstituto Mexicano del Seguro Social)GuadalajaraMéxico University Health Sciences Centre University of Guadalajara(Centro Universitario de Ciencias de la SaludUniversidad de Guadalajara)GuadalajaraMéxico University Centre for Exact and Engineering Sciences University of Guadalajara(Centro Universitario de Ciencias Exactas e IngenieríasUniversidad de Guadalajara)GuadalajaraMéxico Pathology and Oxidative Stress Laboratory Division of NeurosciencesOccidental Biomedical Research CentreSocial Security Institute of Mexico(Laboratorio de Estrés Oxidativo&PatologíaDivision de NeurocienciasCentro de Investigacion Biomédica de OccidenteInstituto Mexicano del Seguro Social)GuadalajaraMéxico

Introduction: In bile duct injuries (BDI), cholestasis and cholangitis can alter the fibrinolytic system by promoting an increase of extracellular matrix depositions which favor an imbalance between metalloproteinases (MMPs) and their tissue inhibitors (TIMPs). Materials and Methods: Levels of PAI-1, MMP-3, MMP-8, TIMP-1 and TIMP-2 in 35 patients with post-cholecystectomy BDI by complete biliary obstruction were measured and compared to a healthy control group. Sirius red staining and immune staining for MMP-3 and MMP-8 were also undertaken in liver biopsies. Results: Levels of PAI-1, TIMP-1, TIMP-2 and MMP-8 were higher in BDI than healthy controls: 15 ± 2 ng/mL vs 7.1 ± 2 ng/mL (p 0.024);539 ± 64 ng/mL vs 256 ± 13 ng/mL (p p p 2 vs. 22865.7 ± 3865 μm2 in healthy controls (p 2 vs. 30744.2 ± 5810.2 μm2 (p 2 vs. 116337.9 ± 24803.3 μm2 (p 0.55). These results suggest an imbalance between fibrogenic/fibrinolytic protein levels. Interestingly, expression of the fibrinolytic protein MMP-8 was increased in serum and liver biopsies in BDI. Conclusion: We found an imbalance of profibrogenic molecules which promote extracellular matrix deposition. The over-expression of fibrinolytic proteins such as MMP-8 could limit liver fibrosis, preventing hepatic dysfunction in post-cholecystectomy BDI.

关键词： Cholecystectomy Bile Duct Injury(BDI) Plasminogen Activator Inhibitor Type 1(PAI-1) Tissue Inhibitors of Metalloproteinases(TIMP’s) Metalloproteinases(MMP’s)

Sensitivity,specificity and predictive values of anterior chamber tap in cases of bacterial endophthalmitis

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Eye and Vision 2017年第1期4卷 100-105页

作者： Carl Sjoholm-Gomez de Liano Vidal F.Soberon-Ventura Guillermo Salcedo-Villanueva Abril Santos-Palacios jose luis Guerrero-Naranjo Jans Fromow-Guerra Gerardo García-Aguirre Virgilio Morales-Canton Raul Velez-Montoya Retina Department Asociación para Evitar la Ceguera en MexicoHospital“Dr.Luis Sanchez Bulnes”IAPMexico CityMexico Universidad Popular Autonoma del Estado de Puebla PueblaMexico Ophthalmology Department Macula Retina ConsultantsMexico CityMexico

Background:To assess the sensitivity,specificity,positive predictive value and negative predictive value of anterior chamber tap for the diagnosis of bacterial endophthalmitis on a population with high ***:Retrospective,single centre,case series *** reviewed all medical records with clinical diagnosis of bacterial endophthalmitis in our hospital from January 1st,2000 to December 31st *** each record,we documented general demographic data,best corrected visual acuity and vitreous and aqueous tap microbiological *** cases were further divided according to the endophthalmitis aetiology to perform individual calculations of sensitivity,specificity,positive predictive value,negative predictive value,accuracy and *** used the results of the vitreous tap as the gold standard for diagnosis of bacterial *** excluded those records in which the aqueous and vitreous samples were not taken simultaneously or had an incomplete microbiological *** were assessed with chi squared statistics,with an alpha value of 0.05 for statistical ***:A total of 190 cases fulfilled the inclusion/exclusion *** culture rate from vitreous samples was 64.74%.Positive culture rate from aqueous sample was 32.11%.Bacteria isolated from aqueous samples matched those isolated from vitreous samples 78.68%of the *** overall sensitivity was 38.21%,specificity:75.51%,positive predictive value:79.66%,negative predictive value:32.74%(p=0.08).Subgroup analysis showed that anterior chamber taps in cases of post-surgical endophthalmitis had a moderate to low sensitivity(37.73%),high specificity(93%)and high positive predictive value(95%)(p<0.04).Conclusion:The sensitivity and specificity of anterior chamber tap are low and should not be used for critical therapeutic decisions in patients with suspected bacterial *** cases of post-surgical endophthalmitis,the result of an anterior chamber tap

关键词： Aqueous humour Endophthalmitis Anterior chamber tap Diagnostic test Vitreous tap Aqueous sampling Sensibility Sensitivity

Towards Bio-impedance Based Labs： A Review

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Journal of Electrical Engineering 2016年第3期4卷 116-127页

作者： Pablo Perez Andres Maldonado Alberto Yufera Gloria Huertas Adoracion Rueda jose luis Huertas Instituto de Microelectr6nica de Sevilla Universidad de Sevilla CSIC Sevilla 41092 Spain Dpt. Electronic Technology ETSIL Universidad de Sevilla Sevilla 41012 Spain Dpt. Electronic and Electromagnetism F. Physics Universidad de Sevilla Sevilla 41012 Spain

In this article, some of the main contributions to BI （Bio-Impedance） parameter-based systems for medical, biological and industrial fields, oriented to develop micro laboratory systems are summarized. These small systems are enabled by the development of new measurement techniques and systems （labs）, based on the impedance as biomarker. The electrical properties of the life mater allow the straightforward, low cost and usually non-invasive measurement methods to define its status or value, with the possibility to know its time evolution. This work proposes a review of bio-impedance based methods being employed to develop new LoC （Lab-on-a-Chips） systems, and some open problems identified as main research challenges, such as, the accuracy limits of measurements techniques, the role of the microelectrode-biological impedance modeling in measurements and system portability specifications demanded for many applications.

关键词： Bio-impedance sensing signal conditioning circuits microelectrode OBT (Oscillation Based Test) LoC.

Proposal for a Project of Integration of IT Tools With Focus on Planning and Control of Operations on the Shop Floor

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Management Studies 2016年第3期4卷 111-130页

作者： Walther Azzolini Junior jose luis Garcia Hermosila Antonio Marcos Vitoreli Rubens Parada Neto State University of Sao Paulo Sao Carlos Brazil University Center of Araraquara Araraquara Brazil

Manufacturing system, with high level of complexity and with a mix of semi-repetitive and repetitive products, to become productive, should seek the standardization of products and processes to obtain the optimization of use of production resources. However, it is necessary to measure the productivity, so that the system of measurement and control of manufacturing processes are an element critical as to ensure greater visibility of the flow＇s restrictions, minimized when detected properly. In this case, the automation of factory＇s measurement process can effectively contribute to ensuring the effectiveness of the function control of a manufacturing system. It is important to consider that the automation of the system of measurement and control of manufacturing processes, of complex environment, is heavily dependent of IT tools applied directly in the interface computational between the operation systems and the corporate systems. This heavy reliance, if exploited technically properly, allows that automation of the system of measurement and control of production makes the access to time real of availability of manufacturing process＇s data, such as processing time and setup time that it can export to a specialist software in programming production, for example, feasible. In this paper, the automation of the system of measurement and control of production is approached, in order to identify the main possibilities of the design of an information system capable to integrate the flow of information in an environment internal on manufacturing organizations, with emphasis in the digital manufacturing paradigm.

关键词： advanced planning system operations management sales and operations planning shop floor control,supply chain planning

Serial Ultrasonographic Measurements to Estimate Age and Viability in <i>Bos indicus</i>Embryos

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Open Journal of Veterinary Medicine 2019年第7期9卷 91-99页

作者： Ana Lucia Malvaez Carlos Salvador Galina Ivette Gutierrez Manuel Corro jose luis Pablos Departamento de Reproducción Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional Autónoma de México Ciudad Universitaria Ciudad de México México Centro de Enseñ anza Investigación y Extensión en Ganadería Tropical Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional Autónoma de México Tlapacoyan Veracruz México Departamento de Genética y Bioestadística Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional Autónoma de México Ciudad Universitaria Ciudad de México México

Early pregnancy diagnosis is a very important routine management to identify non-pregnant cows in order to keep an optimum reproductive efficiency both for dairy and beef cows. Ultrasound equipment allows estimating the viability and size of the embryo during early gestation. With the aim of assessing age and viability of Bos indicus embryos between 24 and 35 days of gestation, 55 cows were divided into 4 clusters. These clusters were conformed according to the first day that an echogenic structure was seen inside the embryonic vesicle (Group 1: day 24, Group 2: day 26, Group 3: day 27 and Group 4: day 28). The age of the embryos was estimated from the date of AI. Three progressive serial measurements of their length were made within 2 to 5 days. With a model GEE (Generalized Estimating Equations), the effect of days was evaluated to compare the second measurement with the first one and the former with the third observation. No differences in the size of the embryos were observed between the first evaluation (24 days) and the second (28 days) (P = 0.387). Also, simple linear regression analysis found a positive linear association between the size of the embryo and gestation days (R2 = 0.593) with an average growth of 0.078 cm per day (P = 0.001). In conclusion, the variation between measurements at a given time does not allow estimating with precision the exact day of gestation. Nonetheless, serial measurements are useful to estimate the healthy growth of the embryo from day 26 of gestation.

关键词： Zebu Cattle Embryo Measurement Reproduction Tropics

Electron Paramagnetic Resonance of Gd3＋ in Cs 2Na Y0.75 H o0.25CI6

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材料科学与工程（中英文B版） 2011年第3期1卷 352-357页

作者： josefina Maldonado Jorge Barreto jose luis Boldu Hector del Castillo Eduardo Mufioz Picone Instituto de Fisica Universidad Nacional Autonoma de Mexico Circuito de la Investigacion Cientifica Ciudad Universitaria D.F. 04510 Mexico

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索尼亚”:致命女人

模型世界 2018年第9期 20-27页

作者： jose luis Etxaide

我对所有的飞机都非常的痴迷,但是日本飞机无疑是我的最爱。特别是那些来自20世纪30年代和40年代的,它们带有着独特的设计。我所选择的这个模型是一架属于日本陆军的三菱Ki-51俯冲轰炸机,它同样还被特殊部队用于自杀式(kamikaze)攻击。... 详细信息

我对所有的飞机都非常的痴迷,但是日本飞机无疑是我的最爱。特别是那些来自20世纪30年代和40年代的,它们带有着独特的设计。我所选择的这个模型是一架属于日本陆军的三菱Ki-51俯冲轰炸机,它同样还被特殊部队用于自杀式(kamikaze)攻击。模型我选择了1/48比例中唯一一款有这个型号飞机的模型套件,它来自Nichimo。这是款老模型了,不过外形相当准确,M旧由完家Gr

关键词： AK 着陆灯阴影效果驾驶舱机翼前缘橡皮筋驾驶员座椅

Compound Hidden Markov Model for Activity Labelling

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International Journal of Intelligence Science 2015年第5期5卷 177-195页

作者： jose Israel Figueroa-Angulo Jesus Savage Ernesto Bribiesca Boris Escalante luis Enrique Sucar Biorobotics Laboratory Universidad Nacional Autonoma de Mexico Mexico City Mexico Computer Science Department Universidad Nacional Autonoma de Mexico Mexico City Mexico Electrical Engineer Department Universidad Nacional Autonoma de Mexico Mexico City Mexico Computer Science Department Instituto Nacional de Astrofisica Optica y Electronica Puebla Mexico

This research presents a novel way of labelling human activities from the skeleton output computed from RGB-D data from vision-based motion capture systems. The activities are labelled by means of a Compound Hidden Markov Model. The linkage of several Linear Hidden Markov Models to common states, makes a Compound Hidden Markov Model. Each separate Linear Hidden Markov Model has motion information of a human activity. The sequence of most likely states, from a sequence of observations, indicates which activities are performed by a person in an interval of time. The purpose of this research is to provide a service robot with the capability of human activity awareness, which can be used for action planning with implicit and indirect Human-Robot Interaction. The proposed Compound Hidden Markov Model, made of Linear Hidden Markov Models per activity, labels activities from unknown subjects with an average accuracy of 59.37%, which is higher than the average labelling accuracy for activities of unknown subjects of an Ergodic Hidden Markov Model (6.25%), and a Compound Hidden Markov Model with activities modelled by a single state (18.75%).

关键词： Hidden Markov Model Compound Hidden Markov Model Activity Recognition Human Activity Human Motion Motion Capture Skeleton Computer Vision Machine Learning Motion Analysis

Synthesis and Characterization of ZnO Film on Stainless Steel Wire and Bioaerosol Antimicrobial Activity Evaluation

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Journal of Environmental Science and Engineering(A) 2015年第9期4卷 455-466页

作者： Alfonso Espitia Cabrera jose luis Contreras Larios IlyaEspitia-Cabrera Ma-Teresa Nunez Cardona Doris Neri Cortes luis Raul Tovar Galvez Energy Department Metropolitan Autonomous University Azcapotzalco 02200 Mexico Chemical Engineering School Michoacan University of San Nicolas of Hidalgo Morelia 58030 Mexico Man and lts Environment Department Metropolitan Autonomous University Xochimilco 04960 Mexico Biochemistry Department Biological Sciences National School National Polytechnic Institute Mexico D.F. 07738 Mexico Interdisciplinary Centre of Research and Studies on Environment and Development National Polytechnic Institute Mexico D.F.07340 Mexico

ZnO thin films were prepared by electrophoretic deposition on stainless steel wire sieve, using zinc acetate as a precursor. The film was sintered and characterised by Scanning Electron Microscopy （SEM）, X-Ray Diffraction （XRD） and chemisorption of oxygen. A culture bacterial consortium composed by gram negative rod-shaped microbes was prepared in a liquid agar in a flask. It was transported by air through a reactor equipped with a UV lamp with 3 and 5 sieves of a stainless steel wire coated with ZnO film. It was exposed in continuous in five experiments to photocatalytic advanced oxidation. The experiments showed a total efficiency for colony forming unit reduction of a maximum of 99.66% for a residence time of 20 seconds with 5 stainless steel wire where exposed in continuous to UV. Also they were evaluated at 7.5 seconds, observing that the contribution of residence time and amount of catalytic for the CFU reduction was quite similar. Variance analysis showed that the efficiency was significant with the no parametric Kruskal-Wallis test with P 〈 0.05. This technology could be used to clean indoor air of closed environments such as hospitals, crowded buildings or public transportation systems where airborne bacteria has been documented.

关键词： Photocatalytic oxidation ZnO thin film antibacterial activity ZnO film on stainless steel wire. I