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AMP-activated kinase in human spermatozoa： identification, intracellular localization, and key function in the reRulation of sperm motility

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Asian Journal of Andrology 2017年第6期19卷 707-714页

作者： Violeta Calle-Guisado Ana Hurtado de Llera David Martin-Hidalgo jose mijares Maria C Gil Ignacio S Alvarez Maria J Bragado Luis J Garcia-Marin Research Group of Intracellular Signalling and Technology of Reproduction (SINTREP) School of Veterinary Medicine University of Extremadura Caceres Spain Assisted Reproduction Unit at the-Minimally Invasive surgery Center Jesos Uson (CCMIJU) Caceres Spain Norba Clinic Caceres Spain Research Group of Reproduction and Embryo Development (REDES) University of Extremadura Badajoz Spain Extremadura Institute of Assisted Reproduction (IERA) Badajoz Spain.

AMP-activated kinase （AMPK）, a protein that regulates energy balance and metabolism, has recently been identified in boar spermatozoa where regulates key functional sperm processes essential for fertilization. This work＇s aims are AMPK identification, intracellular localization, and their role in human spermatozoa function. Semen was obtained from healthy human donors. Sperm AMPK and phospho-Thr172-AMPK were analyzed by Western blotting and indirect immunofluorescence. High- and low-quality sperm populations were separated by a 40%-80% density gradient. Human spermatozoa motility was evaluated by an Integrated Semen Analysis System （ISAS） in the presence or absence of the AMPK inhibitor compound C （CC）. AMPK is localized along the human spermatozoa, at the entire acrosome, midpiece and tail with variable intensity, whereas its active form, phospho-Thr172-AMPK, shows a prominent staining at the acrosome and sperm tail with a weaker staining in the midpiece and the postacrosomal region. Interestingly, spermatozoa bearing an excess residual cytoplasm show strong AMPK staining in this subcellular compartment. Both AMPK and phospho-Thr172-AMPK human spermatozoa contents exhibit important individual variations. Moreover, active AMPK is predominant in the high motility sperm population, where shows a stronger intensity compared with the low motility sperm population. Inhibition of AMPK activity in human spermatozoa by CC treatment leads to a significant reduction in any sperm motility parameter analyzed： percent of motile sperm, sperm velocities, progressivity, and other motility coefficients. This work identifies and points out AMPK as a new molecular mechanism involved in human spermatozoa motility. Further AMPK implications in the clinical efficiency of assisted reproduction and in other reproductive areas need to be studied.

关键词： AMP-activated kinase human spermatozoa immunolocalization sperm motility sperm quality

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Ensuring Quality of Random Numbers from TRNG: Design and Evaluation of Post-Processing Using Genetic Algorithm

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Journal of Computer and Communications 2016年第4期4卷 73-92页

作者： jose J. mijares Chan Parimala Thulasiraman Gabriel Thomas Ruppa Thulasiram Electrical and Computer Engineering Department-Faculty of Engineering University of Manitoba Winnipeg Canada Computer Science Department Faculty of Science University of Manitoba Winnipeg Canada

Random numbers generated by pseudo-random and true random number generators (TRNG) are used in a wide variety of important applications. A TRNG relies on a non-deterministic source to sample random numbers. In this paper, we improve the post-processing stage of TRNGs using a heuristic evolutionary algorithm. Our post-processing algorithm decomposes the problem of improving the quality of random numbers into two phases: (i) Exact Histogram Equalization: it modifies the random numbers distribution with a specified output distribution;(ii) Stationarity Enforcement: using genetic algorithms, the output of (ii) is permuted until the random numbers meet wide-sense stationarity. We ensure that the quality of the numbers generated from the genetic algorithm is within a specified level of error defined by the user. We parallelize the genetic algorithm for improved performance. The post-processing is based on the power spectral density of the generated numbers used as a metric. We propose guideline parameters for the evolutionary algorithm to ensure fast convergence, within the first 100 generations, with a standard deviation over the specified quality level of less than 0.45. We also include a TestU01 evaluation over the random numbers generated.

关键词： True Random Number Generators Genetic Algorithms Auto-Correlation Entropy Power Spectral Density

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阑尾绞痛

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宁夏医科大学学报 1986年第2期 83-83,92页

作者： jose A. mijares 高铁君

在美国急性阑尾炎死亡率稳定下降,但每年仍有近千名患者包括百名儿童死于阑尾炎,这主要是延误诊断及对并发症的严重性认识不足的结果。本文目的使大家对阑尾疾病重新引起兴趣、更好地理解其病理生理、早期诊断、早期治疗。主张在阑尾出... 详细信息

在美国急性阑尾炎死亡率稳定下降,但每年仍有近千名患者包括百名儿童死于阑尾炎,这主要是延误诊断及对并发症的严重性认识不足的结果。本文目的使大家对阑尾疾病重新引起兴趣、更好地理解其病理生理、早期诊断、早期治疗。主张在阑尾出现完全梗阻或出现急

关键词：阑尾阑尾腔病理生理

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