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Real-time cell analysis and heat shock protein gene expression in the TcA Tribofium castaneum cell line in response to environmental stress conditions

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Insect Science 2017年第3期24卷 358-370页

作者： Andres Garcia-Reina Maria Juliana Rodriguez-Garcia Guillermo Ramis jose galian Department of Zoology and Physical Anthropology University of MurciaCampus Mare Nostrum E-30100 Murcia Spain Department of Animal Production Faculty of Veterinary University of MurciaCampus Mare Nostrum E-30100 Murcia Spain

The rust red flour beetle, Tribolium castaneum （Herbst, 1797）（Coleoptera： Tenebrionidae）, is a pest of stored grain and one of the most studied insect model species. Some of the previous studies involved heat response studies in terms of survival and heat shock protein expression, which are regulated to protect other proteins against environ- mental stress conditions. In the present study, we characterize the impedance profile with the xCELLigence Real-Time Cell Analyzer and study the effect of increased temperature in cell growth and viability in the cell line BCIRL-TcA-CLG 1 （TcA） of T castaneum. This novel system measures cells behavior in real time and is applied for the first time to insect cells. Additionally, cells are exposed to heat shock, increased salinity, acidic pH and UV-A light with the aim of measuring the expression levels of lisp27, Hsp68a, and Hsp83 genes. Results show a high thermotolerance of TeA in terms of cell growth and viability. This result is likely related to gene expression results in which a significant up-regulation of all studied Hsp genes is observed after 1 h of exposure to 40 ~C and UV light. All 3 genes show similar expression patterns, but Hsp27 seems to be the most affected. The results of this study validate the RTCA method and reveal the utility of insect cell lines, real-time analysis and gene expression studies to better understand the physiological response of insect cells, with potential applications in different fields of biology such as conservation biology and pest management.

关键词： environmental stress gene expression heat shock proteins insect cells,real-time cell analysis （RTCA） red flour beetle

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Controlled building of CdSe@ZnS/Au and CdSe@ZnS/ Au2S/Au nanohybrids

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Nano Research 2015年第7期8卷 2271-2287页

作者： Raquel E. galian Pilar Diaz Antonio Ribera Alejandro Rincon-Bertolin Said Agouram Julia Perez-Prieto Instituto de Ciencia Molecular (ICMol) Universidad de Valencia c/ Catedrdtico Jose Beltran 2 46980 Paterna Valencia Spain Department of Applied Physics and Electromagnetism Dr. Moliner 50 Universidad de Valencia 461 00 Burjassot Valencia Spain

The addition of Au3. to spherical amine-capped CdSe@ZnS nanoparticles in toluene at room temperature and under darkness can lead to ternary CdSe@ZnS/Au nanohybrids. We demonstrate that this happens only when the nanoparticles possess a relatively thin ZnS shell, thus showing that thickness plays a key role in gold deposition on the CdSe@ZnS nanoparticle surface. Our hypothesis is that the amine ligand acts as the reductant of Au3＋ ions into Au＋ ions, whose affinity for sulfur would keep them at the CdSe@ZnS surface. This interaction stabilizes the Au＋ ion, making it less prone to reduction than a non-coordinated Au＋ ion. In CdSe@ZnS with a thin shell, Au＋ ions at the surface of, or most probably within, the ZnS shell cause the transfer of Cd2＋ ions into the solution. Subsequently, the core Se2- anion, which is a better reductant than the shell S2- reduces Au＋ ions to Au（0）, and large gold nanoparticles （AuNPs） are quickly deposited on the CdSe@ZnS surface in room temperature process, leading to ternary CdSe@ZnS/Au nanohybrids. In solution, these ternary nanohybrids progressively transform into quaternary CdSe@ZnS/Au2S/Au nanohybrids due to the reaction of the shell S2- anion with the remaining Au＋ at the CdSe@ZnS surface, thus leading to the growth of Au2S nanoparticles on the CdSe@ZnS surface while Zn concomitantly leaches from the nanohybrid into the solution. Photoirradiation of the heterostructures with visible light enhances their emission efficiency. Comparatively, irradiation of the precursors, i.e., CdSe@ZnS nanoparticles, causes a drastic decrease in their emission accompanied by a blue shift of their emission maximum. The optical properties of these nanohybrids were analyzed by absorption and fluorescence （steady-state and time-resolved） spectroscopy, and the composition of the samples and the chemical states were determined by energy dispersive X-ray spectroscopy （EDX） and X-ray photoelectron spectroscopy （XPS）, respectively. Finally, the structural and morphological characterizations of the nanohybrid

关键词： heterostructures semiconductor nanoparticle metallic fluorescence time-resolved studies

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