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Justicia insularis Improves the in vitro Survival and Development of Ovine Preantral Follicles Enclosed in Ovarian Tissue

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Journal of Pharmacy and Pharmacology 2017年第9期5卷 668-680页

作者： Gildas Tetaping Mbemya Denise Damasceno Guerreiro Nathalie Jiatsa Donfacl~ Luciana Mascena Silv~ Luis Alberto Vieira Geovania Francisca Canafistula de Sousa Benner Geraldo Alves Aryele Pinto Izaguirry Francielli Weber Santos Phelix Bruno Telefo Otilia Deusdenia Loiola Pessoa Johan Smitz jose Ricardo de Figueiredo Ana Paula Ribeiro rodrigues Faculty of Veterinary (FA VET) State University of Ceara Fortaleza 60714903 Brazil Laboratory of Reproduction Biotechnology (Biotech) State of University of Pampa Uruguaiana 97500970 Brazil Laboratory of Biochemistry of Medicinal Plants Food and Nutritional Sciences Faculty of Science University of DschangDschang 3451381 Cameroon Laboratory of Phytochemical Analysis of Medicinal Plants (LAFIPLAN) Federal University of Cear6 Fortaleza 60020181 Brazil Follicle Biology Laboratory Center for Reproductive Medicine Brussels 1090 Belgium

Objectives： Evaluating the addition effect of./. insularis extract and FSH on the survival, activation and ROS production after in vitro culture of ovine preantral follicles enclosed in ovarian tissue. Methods： In the first experiment, ovarian fragments were fixed （non-cultured control） or in vitro cultured in α-MEM＋（cultured control）, α-MEM＋ supplemented with FSH 50 ng/mL, or in α-MEM＋supplemented with J. insularis （JUS0.3; 1.25 or 5 mg/mL） for 1 or 7 days, at 39℃, 5% CO2. In the second experiment, fragments were fixed or cultured in α-MEM＋ supplemented with anethole 300 μg/mL ＋ FSH 50 ng/mL or in α-MEM＋ supplemented with anethole 300μg/mL ＋ 0.3 mg/mL JUS. Key findings： JUS0.3 was the only treatment that maintained the percentage of morphologically normal follicles similar to non-cultured control even after 7 days of culture. After 7 days of culture, a higher （p 〈 0.05） percentage of developing follicles was observed in JUS5 treatment compared with the other treatments except JUS 1.25. In the second experiment, FSH maintained the percentage of normal follicles and promoted activation of primordial follicles. A reduction （p 〈 0.05） of stromal cell density was observed in MEM＋＋ANE supplemented with JUS or FSH. Conclusions： J. insularis in a concentration-dependent manner maintained the levels of ROS and improved in vitro follicular survival and activation of ovine primordial follicles.

关键词： Medicinal plant antioxidant in vitro folliculogenesis preantral follicles.

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Behavioral study with Erythrina velutina fractions

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Asian Pacific Journal of Tropical Medicine 2009年第5期2卷 30-33页

作者： Silvania Maria Mendes Vasconcelos Natalia Martins Lima Lucas Siqueira de Lucena Alexandre Cesar Praxedes rodrigues Edilberto Rocha Silveira Carlos Clayton Torres Aguiar Paula Matias Soares Francisca Clea Florenco de Sousa Glauce Socorro de Barros Viana Department of Physiology and Pharmacology Federal University of CearaRua Cel.Nunes de Melo 112760431-270FortalezaCearaBrazil Department of Organic Chemistry Federal University of CearaAv.Mister Hull60455-760FortalezaCearaBrazil

Objective:To study the behavioral effects of semi purified fraction prepared from the stem bark of Erythrina velutina on ***:Three chloroformic fractions(CA) at 50 or 100 mg/kg(CA1,CA2 and CA3) were tested in two behavioral tests;plus maze and open ***:In the plus maze test,it was observed a decrease in the number of entries in the closed arms(NECA) CA2 at both doses as compared to the control *** the open field test,CA2 and CA3 fractions decreased the number of squares crossed and the time of permanence on the bar,increased the number of falls at both doses in the rota rod *** CA2 fraction decreased the NECA,but this data did not characterize an anxiolytic *** was the consequence from decrease of locomotor activity(LA) observed in the open field test,that the decrease of LA and rearing,the number of falls and the decrease in time of permanence on the bar in rota rod test were observed after treatment with CA2 and CA3 at both doses,suggesting sedative ***:The present work demonstrated that CA2 and CA3 fractions have impairing effect on LA and motor coordination.

关键词： Erythrina relutina Fraction Locomotor activity Motor coordination

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Bone Remodeling, Biomaterials And Technological Applications: Revisiting Basic Concepts

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Journal of Biomaterials and Nanobiotechnology 2011年第3期2卷 318-328页

作者： Patricia C.Salgado Plinio C.Sathler Helena C.Castro Gutemberg G.Alves Aline M.de Oliveira Rodrigo Cde Oliveira Monica D.C.Maia Carlos R.rodrigues Paulo G.Coelho Andre Fuly Lúcio M.Cabral jose M.Granjeiro Program of Post-Graduation in Medical Sciences Fluminense Federal UniversityNiteróiBrazil Post-Graduation in Biology of Interactions Institute of BiologyFluminense Federal UniversityNiteróiBrazil Program of Post-Graduation in Pathology Fluminense Federal UniversityNiteróiBrazil Clinical Research Unit Antonio Pedro HospitalNucleus of Cell TherapyFluminense Federal UniversityNiteróiBrazil School of Odontology Fluminense Federal UniversityNiteróiBrazil Department of Biological Sciences Dental School of BauruSão Paulo UniversitySão PauloBrazil School of Pharmacy Federal University of Rio de JaneiroRio de JaneiroBrazil Department of Biomaterials and Biomimetics New York UniversityNew YorkUSA

Presently, several different graft materials are employed in regenerative or corrective bone surgery. However current misconceptions about these biomaterials, their use and risks may compromise their correct application and development. To unveil these misconceptions, this work briefly reviewed concepts about bone remodeling, grafts classification and manufacturing processes, with a special focus on calcium phosphate materials as an example of a current employed biomaterial. Thus a search on the last decade was performed in Medline, LILACS, Scielo and other scientific electronic libraries using as keywords biomaterials, bone remodeling, regeneration, biocompatible materials, hydroxyapatite and therapeutic risks. Our search showed not only an accelerated biotechnological development that brought significant advances to biomaterials use on bone remodeling treatments but also several therapeutic risks that should not be ignored. The biomaterials specificity and limitations to clinical application point to the current need for developing safer products with better interactions with the biological microenvironments.

关键词： Biocompatible materials Bone remodeling Hydroxyapatite Therapeutic risks

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Climatological Hydric Balance and the Trends Analysis Climatic in the Region of Machado in Minas Gerais State,Brazil

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American Journal of Climate Change 2018年第4期7卷 558-574页

作者： Gustavo Souza.rodrigues Fernando Ferrari Putti Antonio Carlos da Silva Alisson Souza de Oliveira Luís Roberto Almeida Gabriel Filho jose Rosário Vellano University(UNIFENAS) AlfenasBrazil Machado Higher Education and Research Center(CESEP) MachadoBrazil Paulista State University Júlio de Mesquita Filho(UNESP) BotucatuBrazil

Lately the planet’s climate has been constantly changing, caused mainly by global warming which has exposed a great deal of concern to the population over the years. In order to understand the possible impacts that such changes may have on the environment and society in general, the importance of the analysis of climate and hydrological events trends and their performance in a region is justified. The objective of the present work was to perform the climatic classification and to evaluate the behavior of the Climatological Hydric Balance—CHB, from the region of Machado state of Minas Gerais—MG, taking into account a historical series of 55 years of climatic season data of the National Institute of Meteorology—INMET;to verify the occurrence of climatic changes by the temporal trends of precipitation and the average temperature, using the Mann-Kendall and Pettitt method;and the influence of these possible climate changes on CHB behavior and on the region’s climate classification. Based on the results found it verified the increase in the water deficit between the months of June to September and a reduction in the water surplus from November to February. By means of the trend analysis, there was a positive trend of increase in the average temperature of 1.6°C until the year 2100. The continuity and occurrence of these trends may have impacts on the economy, agriculture, the hydrological cycle, and consequently on the fauna, the flora and the population.

关键词： Climatic Series Global Warming IPCC

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医院小丑在儿科症状管理中的效果

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英国医学杂志中文版 2021年第10期24卷 606-608页

作者： Lufs Carlos Lopes-Junior Emiliana Bomfim Karin Olson Eliane Tatsch Neves Denise Sayuri Calheiros Silveira Michelle Darezzo rodrigues nunes Lucila Castanheira Nascimento Gabriela Pereira-da-Silva Regina Aparccida Garcia Lima 蔡思雨(译) 彭晓霞(校) Federal University of Espirito Santo Avenida Marechai CampusES.Brazil University of Saskatchewan College of Medicine.SaskatoonSKCanada University of Alberta EdmontonABCanada Federal University of Santa Maria Santa Maria.RSBrazil Federal Institute of Espirito Santo LinharesESBrazil Rio de Janeiro State University Rio de Janeiro.RJ.Brazil University of Sao Paulo at Ribeirao Prelo College of Nursing SPBrazil 国家儿童医学中心首都医科大学附属北京儿童医院临床流行病学与循证医学中心

目的基于随机对照试验和非随机对照试验产生的证据,在因急慢性疾病住院的儿童和青少年中,评价医院小丑对一系列症状群的干预效果。研究设计基于随机和非随机对照试验的系统综述。数据来源美国国立医学图书馆(MEDLINE).美国科学情报研究... 详细信息

目的基于随机对照试验和非随机对照试验产生的证据,在因急慢性疾病住院的儿童和青少年中,评价医院小丑对一系列症状群的干预效果。研究设计基于随机和非随机对照试验的系统综述。数据来源美国国立医学图书馆(MEDLINE).美国科学情报研究所数据库(ISI of Knowledge).考科蓝对照试验注册中心(Cochrane Cental Registerof Controled Trials)、Science Diret全文数据库、Sopus数据库、美国心理协会数据库(American Psychalogical Association PeyeINFO)、护理和健康文献累积指数数据库(Curmulative Index to Nursing and Alied Heauth Literature),以及拉丁美洲和加勒比健康科学文献数据库(Lain Ameriean and Caribbean Health Sciences Literature)。研究筛选经間行评议后筛选随机和非随机对照试脸,纳人标准如下:因急性或慢性疾病人院的儿童和青少年,比较医院小丑与标准护理的研究,此外研究将医院小丑对住院儿童和青少年症状管理的影响作为主要结局指标。数据提取与整合由两名研究者独立完成研究筛选,数据摘录,以及偏倚风险评价。方法学评价也由两名研究者独立进行,评价工具有:Jadnd量表、随机对照试验的偏倚风险评估工具(RoB 2)和非随机对照试验的偏倚风险评估工具(ROBINS-I)。结果24项研究(n=1612)符合纳入标准,对其进行数据摘录和分析。大多数研究是随机对照试验(n=13)。最常分析的症状是焦虑(n=13),其次是疼痛(n=9)、心理和情绪反应以及幸福感(n=4)、压力(n=4)、癌症相关疲劳(n-3)和哭泣(n=2)。5项研究使用生物标志物(主要为皮质醇)来评估医院小丑对压力或疲劳的影响。方法学评价显示,大多数随机对照试验(n=11;85%)存在潜在偏倚风险,2项试验具有高度偏倚风险。ROBINS-I评价结果显示,大多数非随机对照试验(n=6;55%)存在中度偏倚风险。研究表明,无论父母是否在场,医院小丑出现时,儿童和青少年在一系列医疗程序中的焦虑显著降低,心理调节也得到改善(P<0.05)。3项针对慢性病患者的研究显示,医院小丑的干预有明显获益,显著减少了患儿的压力、疲劳、疼痛和痛营(PC0.05)。结论这些研究表明,在医疗程序术前诱导麻醉,以及慢性病常规护理中进行医院小丑干预,可能是症状管理的有效策略。此外,与接受标准护理的患儿相比,医院小丑干预可能有助于改善患有急慢性疾病的儿童和青少年的心理健康状态。

关键词：急慢性疾病方法学评价慢性病患者心理健康状态诱导麻醉症状群情报研究所文献数据库

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化学或物理催化激活方式对高浓度漂白剂的影响

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中国口腔医学继续教育杂志 2012年第4期15卷 235-241页

作者： Debora Alves nunes Leite Lima Flavio Henrique Baggio Aguiar Priscila C. Suzy Liporoni Egberto Munin Glacia Maria Bovi Ambrosano jose Roberto Lovadino 陈铭晟(译) 孟玉坤(审) 四川大学华西口腔医学院修复学系四川省成都市人民南路三段14号 610041

目的：用体外研究方法评价采用化学或物理催化激活的高浓度漂白剂的漂白效力。材料和方法：本研究分为两部分。第一部分．评价不同光固化设备激活时35％过氧化氢（Whiteness HP Maxx）对牙齿漂白的效力。光固化设备包括：卤素灯（普通... 详细信息

目的：用体外研究方法评价采用化学或物理催化激活的高浓度漂白剂的漂白效力。材料和方法：本研究分为两部分。第一部分．评价不同光固化设备激活时35％过氧化氢（Whiteness HP Maxx）对牙齿漂白的效力。光固化设备包括：卤素灯（普通和漂白模式）（Optilux ***／Kerr），第一代***／二极管激光（Ultrablue Ⅳ．DMC），第二代LED（Bluephase 16i，IvoclarVivadent）和无光源照射组（对照组）。第二部分．分析化学和物理催化方式对高浓度漂白剂的影响．漂白剂包括：35％过氧化氢（Whiteness HP Maxx）＋20％氢氧化钠；35％过氧化氢＋7％碳酸氢钠：38％过氧化氢（Opalescence Xtra Boost）；35％过氧化氢＋卤素灯；35％过氧化氢＋20％氢氧化钠＋卤素灯：35％过氧化氢＋7％碳酸氢钠＋卤素灯：38％过氧化氢＋卤素灯：以及35％过氧化氢。用人离体磨牙制备的块状试件根据不同漂白处理随机分组（n=5）。漂白效果用分光光度计进行测量。漂白过程分为3次。测量结果用ANOVA和Tukeytest进行检验（α=0.05）。结果：两部分实验结果都表明．经催化激活与未经激活的漂白处理．其效果在测试的所有阶段均没有显著性差异。结论：使用激活系统不会改善高浓度漂白剂的漂白效力。

关键词：激活方式物理催化分析化学漂白剂浓度过氧化氢氢氧化钠碳酸氢钠

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Investigation of the Effects of Glazed Balconies uponThermal Comfort in Hot Tropical Region

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Journal of Civil Engineering and Architecture 2019年第12期13卷 762-779页

作者： Érica Coelho Pagel Edna Aparecida Nico rodrigues Cristina Engel de Alvarez Neyval Costa Reis Júnior Bruna Perovano Sirtuli Filipe Galina Costalonga Jordana BernabéCoelho Department of Architecture and City Universidade Vila Velha Av Comissario Jose Dantas De Mello 21Boa Vista IIVila VelhaES29102-920Brazil Department of Architecture and Urbanism Universidade Federal do Espírito SantoAv.Fernando Ferrari 514VitóriaES29060-910Brazil Department of Environmental Engineering Universidade Federal do Espírito SantoAv.Fernando Ferrari 514VitóriaES29060-910Brazil

The balcony is responsible to a series of effects on the environmental behavior of a building,mainly in relation of thermal comfort and air distribution to their indoor *** a very common practice in several metropolitan areas is the closing with retractable glass panels in balcony *** work analyzed the effects of glazed balconies upon thermal comfort in a hot tropical *** parameters were carried out in a flat alternating the conditions of retractable glass panels to *** simulations were performed considering closing or not the glass door that divides the balcony room;the building material used on the balcony sill;and the use of curtains or shading *** results show the maximum mean hourly temperatures recorded on the balcony during the period when the retractable glass panels were closed,reaching peaks between 31.7 and 39.2°C,above the comfort range recommended by ASHRAE *** situations simulated show the thermal discomfort prevails in the use of the closed glass panels reaching DhTD of 94.55°C/***,this practice of using most of the closed retractable glass panels in the balconies presents disadvantages and inefficiencies especially in hot and humid tropical climate regions.

关键词： Glazed balconies thermal comfort hot tropical region.

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Remaining Useful Life Prediction of Li-Po Batteries in UAVs Using Extended Kalman Filter

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Journal of Mechanics Engineering and Automation 2015年第12期5卷 687-690页

作者： Darielson A. Souza Joao L. O. Tortes Joao Paulo P. Gomes Leonardo R. rodrigues Romulo N. de C.Almeida Vandilberto P. Pinto Programa de Pos-Graduacao em Engenharia Eletrica e de Computacao Universidade Federal do Ceara Campus de Sobral Rua Coronel Estanislau Frota s/n Sobral - Ceara 620.010-560 Brazil Departamento de Engenharia Eletrica Universidade Federal do Ceara Campus de Sobral Rua Coronel Estanislau Frota s/n Sobral - Ceara 620. 010-560 Brazil Universidade Federal do Ceara Departamento de Computacao Rua Campus do Pici s/n 620.010-000 Brazil Divisao de Eletronica Instituto de Aeronautica e Espacao Praga Marechal Eduardo Gomes 50 Vila das Acacias Sao Jose dos Campos- SP 12.228-904 Brazil

The use of batteries in UAVs （unmanned aerial vehicles） has become common due to some advantages in comparison with internal combustion engines such as weight reduction and better power control. However, in these vehicles it is critical to monitor the RUL （remaining useful life） of the batteries. This information can be used, for instance, as a decision support tool to define which missions could be assigned to the UAV until the next battery recharge. This work presents a methodology to predict the RUL of Li-Po （Lithium-Polymer） batteries. The approach uses an extended Kalman filter and an exponential model for the degradation evolution. The proposed methodology uses time series of battery terminal voltages, assuming that the discharge occurs under a constant current condition. Different discharge current levels were *** results showed that the proposed methodology provides good results, despite its simplicity.

关键词： Extended Kalman filter Li-Po battery RUL estimation UAV.

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Antibiofilm and immunomodulatory resorbable nanofibrous filing for dental pulp regenerative procedures

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Bioactive Materials 2022年第10期7卷 173-186页

作者： Mauricio Gonçalves da Costa Sousa Gabriela Conceiçao de Almeida Danilo Cesar Martins Mota Rosiane Andrade da Costa Simoni Campos Dias Samuel nunes Limberger Frank Ko Li Ting Lin Evan FHaney Hashem Etayash Beverlie Baquir Michael J.Trimble Ya Shen Zheng Su Markus Haapasalo Daniel Pletzer Letícia Chaves de Souza Glaucia Schuindt Teixeira Renato M.Silva Robert E.W.Hancock Octavio Luiz Franco Taia Maria Berto Rezende Post-Graduation Program in Genomic Sciences and Biotechnology Catholic University of BrasíliaBrasíliaBrazil Dentistry Course Catholic University of BrasíliaBrasíliaBrazil Post-Graduation Program in Health Sciences University of BrasíliaBrazil LIMA Chemistry InstituteUniversity of Brasília-UNBBrasíliaBrazil Animal Biology Department Campus Darcy RibeiroUniversidade de BrasíliaBrazil Department of Materials Engineering Faculty of Applied ScienceUniversity of British ColumbiaVancouverCanada Department of Microbiology and Immunology University of British ColumbiaVancouverBCCanada Department of Oral Biological and Medical Sciences Faculty of DentistryUniversity of British ColumbiaVancouverCanada Department of Microbiology and Immunology University of OtagoDunedinNew Zealand Department of Endodontics School of DentistryUniversity of Texas Health Science Center at HoustonHoustonUSA Department of Prosthesis Faculty of DentistryRio de Janeiro State UniversityRio de JaneiroBrazil Post-Graduation Program in Biotechnology Catholic University Dom BoscoCampo GrandeMato Grosso do SulBrazil

Multifunctional scaffolds with host defense peptides designed for regenerative endodontics are desirable nanobiotechnological tools for ***,different scaffolds were tested for use during the pulp revascularization process,including poly(vinyl alcohol)-PVA hydrogels or resins,collagen hydrogels and poly(vinyl alcohol)PVA/Chitosan(PVA/CS)*** on time to degradation(21 days),nanofibers were chosen to be incorporated with ciprofloxacin and IDR-1002(each at 50 mg/g).Nanofibers containing ciprofloxacin and IDR-1002 had anti-biofilm activity against Enterococcus faecalis,Staphylococcus aureus and a multispecies oral biofilm,besides anti-inflammatory *** in vivo subcutaneous tissue response to tooth fragments filled with nanofibers demonstrated a pulp-like tissue formation,when compared to empty teeth ***,we designed a strong antimicrobial,immunomodulatory and regenerative candidate for pulp revascularization and regeneration procedures.

关键词： Regenerative endodontics Scaffolds Nanofibers Host defense peptides Ciprofloxacin IDR-1002

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Evaluation of antibacterial and toxicological activities of essential oil of Ocimum gratissimum *** its major constituent eugenol

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Food Bioscience 2022年第6期50卷 2152-2159页

作者： Julio Cesar Silva Raimundo Luiz Silva Pereira Thiago Sampaio de Freitas Janaína Esmeraldo Rocha Nair Silva Macedo Carla de Fatima Alves Nonato Marina Leite Linhares Daniely Sampaio Arruda Tavares Francisco Assis Bezerra da Cunha Henrique Douglas Melo Coutinho Sidney Gonçalo de Lima Francisco Nascimento Pereira-Junior Francisco Paulo Araújo Maia Ivo Cavalcante Pita Neto Fabiola Fernandes Galvão rodrigues George Joaquim Garcia Santos Department of Biological Chemistry Regional University of CaririR.Cel.Antonio Luis 116163105000CratoCEBrazil University Center Doutor Leão Sampaio Av.Leão Sampaio 40063040000Juazeiro do NorteCEBrazil Research and Development Center for Medicines Federal University of CearaR.Coronel Nunes de Melo 100060430275FortalezaCEBrazil Chemistry Department Federal University of PiauiCampus Universitário Ministro Petrônio Portella64049550TeresinaPIBrazil Center for Agricultural and Biodiversity Sciences - CCAB Federal University of CaririCratoCearáBrazil CECAPE - College of Dentistry Juazeiro do NorteCE63024-015Brazil

The present study aimed to comparatively analyze the antibacterial activity,combined with antibiotics,and toxicological activity of the essential oil of *** *** its major compound,*** phytochemical analysis of the essential oil was performed by gas chromatography coupled with mass *** minimum inhibitory concentration and combined antibiotic activity were performed by *** melanogaster was the model organism chosen to evaluate the toxicological response of *** chromatographic assay showed eugenol as the major compound of the essential oil of *** *** microbiological assays showed that in terms of antibacterial activity,both products showed similar activity,quite significant in Gram-negatives,such as those of strains of Pseudomonas *** results were verified in the modulatory activity,allowing the verification that the bioactivity performed by the essential oil of *** *** mainly influenced by its major *** the EC50 of the compounds varied subtly,the essential oil was less toxic compared to *** information obtained in the present study demonstrates that the essential oil of *** *** a promising antibacterial agent and better tolerated than its majority compound alone,in the model organism ***.

关键词： Antibacterial agents Drosophila melanogaster Eugenol Ocimum gratisismumum L.essential oils

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