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Multiplexed Biosensing Diagnostic Platforms Detecting Autoantibodies to Tumor-Associated Antigens from Exosomes Released by CRC Cells and Tissue Samples Showed High Diagnostic Ability for Colorectal Cancer

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Engineering 2021年第10期7卷 1393-1412页

作者： Ana Montero-Calle Itziar Aranguren-Abeigon María Garranzo-Asensio Carmen Poves María Jesús Fernández-Aceñero Javier Martínez-Useros Rodrigo Sanz Jana Dziaková Javier Rodriguez-Cobos Guillermo Solís-Fernández Eloy Povedano Maria Gamella Rebeca Magnolia Torrente-Rodríguez Miren Alonso-navarro Vivian de los Ríos J.Ignacio Casal Gemma Domínguez Ana Guzman-Aranguez Alberto Peláez-García josémanuel pingarrón Susana Campuzano Rodrigo Barderas Chronic Disease Program(UFIEC) Carlos III Health InstituteMadrid E-28220Spain Department of Biochemistry and Molecular Biology Faculty of Optics and OptometryComplutense University of MadridMadrid E-28040Spain Gastroenterology Unit San Carlos Clinical HospitalMadrid E-28040Spain Surgical Pathology Department San Carlos Clinical HospitalMadrid E-28040Spain Translational Oncology Division OncoHealth InstituteJimenez Diaz Foundation University HospitalMadrid E-28040Spain Department of Biochemistry Faculty of MedicineAlberto Sols Institute of Biomedical ResearchCSIC-UAMMadrid E-28029Spain Department of Analytical Chemistry Faculty of Chemical SciencesComplutense University of MadridMadrid E-28040Spain Center for Biological Research Spanish National Research Council(CSIC)MadridE-28040Spain Molecular Pathology and Therapeutic Targets Group La Paz University Hospital(IdiPAZ)Madrid E-28046Spain

Colorectal cancer(CRC)is the second leading cause of cancer-related death worldwide.The five-year survival rate of CRC patients depends on the stage at diagnosis,being higher than 80%when CRC is diagnosed in the early stages but lower than 10%when CRC is diagnosed in advanced stages.Autoantibodies against specific CRC autoantigens(tumor-associated antigens(TAAs))in the sera of patients have been widely demonstrated to aid in early diagnosis.Thus,we herein aim to identify autoantigens target of autoantibodies specific to CRC that possess a significant ability to discriminate between CRC patients and healthy individuals by means of liquid biopsy.To that end,we examined the protein content of the exosomes released by five CRC cell lines and tissue samples from CRC patients by means of immunoprecipitation coupled with mass spectrometry analysis.A total of 103 proteins were identified as potential autoantigens specific to CRC.After bioinformatics and meta-analysis,we selected 15 proteins that are more likely to be actual CRC autoantigens in order to evaluate their role in CRC prognosis by Western blot(WB)and immunohistochemistry(IHC).We found dysregulation at the protein level for 11 of these proteins in both tissue and plasma exosome samples from patients,along with an association of nine of these proteins with CRC prognosis.After validation,all but one showed a statistically significant high diagnostic ability to distinguish CRC patients and individuals with premalignant lesions from healthy individuals,either by luminescence Halotag-based beads,or by a multiplexed biosensing platform involving the use of magnetic microcarriers as solid support modified with covalently immobilized Halotag fusion proteins constructed for CRC detection.Taken together,our results highlight the usefulness of the approach defined here to identify the TAAs specific to chronic diseases;they also demonstrate that the measurement of autoantibody levels in plasma against the TAAs identified here could be integrated into a point-of-care(POC)de

关键词： Autoantibodies Diagnosis Colorectal cancer Exosomes Tumor microenviroment Humoral immune response Point of care Biosensors

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Bacteria Detection through Electrochemical Activity Monitoring

Bacteria Detection through Electrochemical Activity Monitori...

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The Eleventh International and the First Sino-Japan Bilateral Symposium on Electroanalytical Chemistry

作者： B.SERRA A.J.REVIEJO J.M.pingarrón Department of Analytical Chemistry Faculty of Chemistry Complutense University of Madrid 28040 Madrid Department of Analytical Chemistry Faculty of Chemistry Complutense University of Madrid 28040 Madrid Department of Analytical Chemistry Faculty of Chemistry Complutense University of Madrid 28040 Madrid

There is an increasing demand for rapid,simple,very sensitive and accurate methods for detecting micro-organisms in fields like drinking water quality assurance,food quality and safety,biological terrorism threat control,applied medicine and fermentation technology.In most of the cases,early detection of very low micro-organisms concentrations is essential for an effective action.

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Enhancement of Corrosion Resistance of niCrFeBSi Coatings Obtained by Flame Thermal Spray Process Adding an Electroless nickel Coating ni-P

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Journal of Surface Engineered Materials and Advanced Technology 2017年第4期7卷 86-97页

作者： Arturo Barba-pingarrón Raúl Valdez-navarro Félix Sánchez-De Jesus Ana María Bolarín-Miró Rafael González-Parra Alba Covelo-Villar Miguel ángel Hernández-Gallegos Carlos Domínguez-Ríos Centro de Ingeniería de Superficies y Acabados (CEnISA) Facultad de Ingeniería Universidad Nacional Autónoma de México 2 Nivel Edificio “T” (Bernardo Quintana) Circuito Exterior Ciudad Universitaria Ciudad de México México área Académica de Ciencias de la Tierra y Materiales Universidad Autónoma del Estado de Hidalgo Pachuca México Centro de Investigación en Materiales Avanzados Chihuahua México

Flame Thermal Spray (FTS) coatings frequently show some porosity and reduced adherence to substrate, which affect its properties, especially its corrosion resistance. In this work, the corrosion resistance of AISI 1018 carbon steel coated by different methods is compared: electroless nickel (En) coating, niCrFeBSi obtained by FTS, duplex coatings of an En deposit on a layer of niCrFeBSi obtained by FTS and a layer of niCrFeBSi on an En deposit. The coatings were characterized using optical microscopy and scanning electron microscopy techniques, EDS microprobe microanalysis, roughness as well as electrochemical polarization tests to obtain the corrosion rate. The results show the enhancement in the corrosion resistance in saline medium of the duplex coatings, especially of the En coating on FTS layer.

关键词： Flame Thermal Spray Coatings Electroless nickel Plating Duplex Coatings Corrosion Resistance

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