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Biofilm analyses and exoproduct release by clinical and environmental isolates of Burkholderia pseudomallei from Brazil

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Asian Pacific journal of Tropical Medicine 2023年第7期16卷 321-328页

作者： Glaucia Morgana de Melo Guedes Crister joséOcadaque Alyne Soares Freitas Rodrigo Machado Pinheiro Giovanna Barbosa Riello Silviane Praciano Bandeira Rossana de Aguiar Cordeiro Marcos Fábio Gadelha Rocha joséjúlio costa sidrim Débora de Souza Collares Maia Castelo-Branco Department of Pathology and Legal Medicine Postgraduate Program in Medical MicrobiologyGroup of Applied Medical MicrobiologyFederal University of CearáFortalezaCEBrazil Department of Clinical and Toxicological Analysis School of PharmacyFederal University of CearáFortalezaCEBrazil Department of Pathology and Legal Medicine Postgraduate Program in Medical MicrobiologyLaboratory of Emerging and Reemerging PathogensFederal University of CearáFortalezaCEBrazil School of Veterinary Medicine Postgraduate Program in Veterinary SciencesState University of CearáFortalezaCEBrazil

Objective:To characterize biofilm production by clinical(n=21)and environmental(n=11)isolates of Burkholderia pseudomallei and evaluate the production of proteases,hemolysins and ***:Initially,the 32 strains were evaluated for biofilm production in Müller-Hinton broth-1%glucose(MH-1%glucose)and BHI broth-1%glucose,using the crystal violet staining ***,growing(48 h)and mature(72 h)biofilms were evaluated by confocal ***,the production of proteases,hemolysins and siderophores by planktonic aggregates,growing biofilms and mature biofilms was ***:All isolates produced biofilms,but clinical isolates had significantly higher biomass in both MH-1%glucose(P<0.001)and BHI-glucose 1%(P=0.005).The structural analyses by confocal microscopy showed thick biofilms,composed of multiple layers of cells,homogeneously arranged,with mature biofilms of clinical isolates presenting higher biomass(P=0.019)and thickness of the entire area(P=0.029),and lower roughness coefficient(P=0.007)than those of environmental *** production by growing biofilms was significantly greater than that of planktonic(P<0.001)and mature biofilms(P<0.001).Hemolysin release by planktonic aggregates was higher than that of biofilms(P<0.001).Regarding siderophores,mature biofilms presented higher production than growing biofilms(P<0.001)and planktonic aggregates(P<0.001).Conclusions:Clinical isolates have higher production of biofilms than their environmental counterparts;protease and siderophores seem important for growth and maintenance of Burkholderia pseudomallei biofilms.

关键词： Mangosteen Burkholderia pseudomallei Biofilms Proteases Hemolysins Siderophores

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Chlamydoconidium-producing Trichophyton tonsurans: Atypical morphological features of strains causing tinea capitis in Ceará, Brazil

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Asian Pacific journal of Tropical Medicine 2019年第8期12卷 380-384页

作者： Raimunda Samia Nogueira Brilhante Germana costa Paixao jonathas Sales de Oliveira Vandbergue Santos Pereira Marcos Fábio Gadelha Rocha Reginaldo Goncalves de Lima-Neto Débora de Souza Collares Maia Castelo-Branco Rossana de Aguiar Cordeiro joséjúlio costa sidrim Specialized Medical Mycology Center Postgraduate Program in Medical MicrobiologyFederal University of CearáRua Nunes de Melo1315FortalezaBrazil Postgraduate Program in Veterinary Sciences State University of CearáAv.Dr.Silas Munguba1700FortalezaBrazil Department of Tropical Medicine Center for Health SciencesFederal University of PernambucoRecife-PEBrazil

Objective: To report atypical morphological features of Trichophyton(T.) tonsurans strains associated with tinea capitis. Methods: Eighty-two T. tonsurans strains isolated in Ceará, Brazil, were analyzed regarding macro and micromorphological features and nutritional patterns. Results: Fifty-two samples presented abundant chlamydoconidia, which were produced in chains. Macroscopically, these strains developed small glabrous colonies that were firmly attached to the surface of the culture medium, with few or no aerial mycelia and intense rusty yellow pigmentation. Seven strains did not grow with stimulus from thiamine. Samples were heterogeneous regarding urease production and none presented in vitro hair perforation. Conclusions: The observation of T. tonsurans strains with distinct phenotypic features indicates the need to revise the taxonomic criteria for routine identification of this dermatophyte.

关键词： Trichophyton tonsurans Chlamydoconidia Dermatophyte Phenotyping

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Enterobacteria and Vibrio from Macrobrachium amazonicum prawn farming in Fortaleza,Ceará,Brazil

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Asian Pacific journal of Tropical Medicine 2016年第1期9卷 24-28页

作者： Débora de Souza Collares Maia Castelo-Branco jamille Alencar Sales Raimunda Samia Nogueira Brilhante Glaucia Morgana de Melo Guedes Yago Brito de Ponte Celia Maria de Souza Sampaio Tereza de jesus Pinheiro Gomes Bandeira josé Luciano Bezerra Moreira Lucas Pereira de Alencar Manoel de Araújo Neto Paiva Rossana de Aguiar Cordeiro André jalles Monteiro Waldemiro de Aquino Pereira-Neto josé júlio costa sidrim Marcos Fábio Gadelha Rocha Department of Pathology and Legal Medicine Postgraduate Program in Medical MicrobiologySpecialized Medical Mycology CenterFederal University of Ceará School of Veterinary Medicine Postgraduate Program in Veterinary SciencesState University of Ceará Department of Statistics and Applied Mathematics Federal University of Ceará

Objective:To investigate the isolation of enterobacteria associated with Macrobrachium amazonicum(***) farming and evaluate the in vitro antimicrobial susceptibility of Vibrio ***:Strains were isolated from female *** prawns and environmental and hatchery *** assays were used to identify bacterial genera and those belonging to the genus Vibrio were submitted to further analyses for species identification,through Vitek 2 automated system and *** test was performed according to Clinical Laboratory Standards ***:The following genera of enlerobacteria were recovered:Enterobacter(n=11),Citrobacter(n=10),Proteus(n=2),Serratia(n=2),Kluyvera(n=2),Providencia(n=2),Cedecea(n=1),Escherichia(n=1).Edwardsiella(n=1) and BulliauxeUa(n=1).As for Vibrio,three species were identified:Vibrio cholerae non-O1/non-O139(n=4).Vibrio vulnificus(***)(n=1) and Vibrio mimicus(n=1).Vibrio *** minimum inhibitory concentrations values within the susceptibility range established by Clinical Laboratory Standards Institute for almost all antibiotics,except for ***,which presented intermediate profile to ***:Enterobacteria do not seem to be the most important pathogens associated with *** farming,whereas the recovery of Vibrio *** larviculture,with emphasis on Vibrio cholerae and ***,deserves special attention due to their role as potentially zoonotic aquaculture-associated ***,the intermediate susceptibility of *** to ampicillin reflects the importance of monitoring drug use in prawn farming.

关键词： Prawn Water Enterobacteria Vibrio Antibiotics

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Inflammatory markers,oxidative stress,and mitochondrial dynamics:Repercussions on coronary artery disease in diabetes

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World journal of Diabetes 2024年第9期15卷 1853-1857页

作者： joséCarlos Tatmatsu-Rocha Luan Santos Mendes-costa College of Medicine Postgraduate Program in Physiotherapy and FunctionalityFederal University of Ceará-UFCFortaleza 60430-450CearáBrazil Physical Therapy Federal University of CearáFortaleza 60000-000CearáBrazil

Inflammatory markers and mediators that affect the development of cardiovascular diseases have been the focus of recent scientific ***,the purpose of this editorial is to promote a critical debate about the article titled“Nε-carboxymethyl-lysine and inflammatory cytokines,markers,and mediators of coronary artery disease progression in diabetes”,published in the World journal of Diabetes in *** work directs us to reflect on the role of advanced glycation end products,which are pro-inflammatory products arising from the metabolism of fatty acids and sugars whose main marker in tissues is Nε-carboxymethyllysine(NML).Recent studies have linked high levels of pro-inflammatory agents with the development of coronary artery disease(CAD),especially tumor necrosis factor alpha,interleukins,and C-reactive *** inflammatory agents increase the production of reactive oxygen species(ROS),of which people with diabetes are known to have an increased *** increase in ROS promotes lipid peroxidation,which causes damage to myocytes,promoting myocardial ***,oxidative stress induces the binding of NML to its receptor RAGE,which in turn activates the nuclear factor-kB,and consequently,inflammatory *** inflammatory cytokines induce endothelial dysfunction,with increased expression of adhesion molecules,changes in endothelial permeability and changes in the expression of nitric *** this sense,the therapeutic use of monoclonal antibodies(inflammatory reducers such as statins and sodium-glucose transport inhibitors)has demonstrated positive results in the regression of atherogenic plaques and consequently *** the other hand,many studies have demonstrated a relationship between mitochondrial dynamics,diabetes,and cardiovascular *** link occurs since ROS have their origin in the imbalance in glucose metabolism that occurs in the mitochondrial matrix,and this imbalance can have its origin in inadequate diet as well as

关键词： Mitochondrial dynamics Diabetes Oxidative stress Coronary artery disease Nε-carboxymethyl-lysine

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Benchmarks for Pirá2.0,a Reading Comprehension Dataset about the Ocean,the Brazilian Coast,and Climate Change

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Data Intelligence 2024年第1期6卷 29-63页

作者： Paulo Pirozelli Marcos M.josé Igor Silveira Flávio Nakasato Sarajane M.Peres Anarosa A.F.Brandão Anna H.R.costa Fabio G.Cozman Universidade de São Paulo Ringgold Standard Institution-Instituto de Estudos Avançados Av.370 São Paulo 05508-900Brazil Universidade de São Paulo Ringgold Standard Institution-Instituto de Matemática e Estatística Av.370 São Paulo 05508-900Brazil Universidade de São Paulo Rua Cayowaá876 apto 141SÃO PAULO 05018-001Brazil Universidade de São Paulo Ringgold Standard Institution Escola Politécnica Av.370São Paulo 05508-900Brazil.

Piráis a reading comprehension dataset focused on the ocean,the Brazilian coast,and climate change,built from a collection of scientific abstracts and reports on these *** dataset represents a versatile language resource,particularly useful for testing the ability of current machine learning models to acquire expert scientific *** its potential,a detailed set of baselines has not yet been developed for Pirá.By creating these baselines,researchers can more easily utilize Piráas a resource for testing machine learning models across a wide range of question answering *** this paper,we define six benchmarks over the Pirádataset,covering closed generative question answering,machine reading comprehension,information retrieval,open question answering,answer triggering,and multiple choice question *** part of this effort,we have also produced a curated version of the original dataset,where we fixed a number of grammar issues,repetitions,and other ***,the dataset has been extended in several new directions,so as to face the aforementioned benchmarks:translation of supporting texts from English into Portuguese,classification labels for answerability,automatic paraphrases of questions and answers,and multiple choice *** results described in this paper provide several points of reference for researchers interested in exploring the challenges provided by the Pirádataset.

关键词： Natural language processing Question answering Benchmarks Language resource DomainOriented dataset Scientific knowledge text dataset

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Structure,stability,catalytic activity,and polarizabilities of small iridium clusters

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Chinese Physics B 2018年第6期27卷 225-230页

作者： Francisco E jorge josé R da costa Venaancio Unidade Academica dc Ffsica Universidade Federal de Campina Grande 58429-900 Campina Grande Brazil Departamento de Ffsica Universidade Federal do Espfrito Santo 29060-900 Vitoria Brazil

At the second order Douglas–Kroll–Hess（DKH2） level, the B3 PW91 functional in conjunction with the relativistic all-electron basis set of valence triple zeta quality plus polarization functions are employed to compute bond lengths, dissociation energies, vertical ionization potentials, and the highest occupied molecular orbital-lowest unoccupied molecular orbital energy gaps of the small iridium clusters（Irn, n≤8）. These results are compared with the experimental and theoretical data available in the literature. Our results confirm the theoretical predictions made by Feng et al. about the catalytic activities of the Ir4 and Ir6 clusters. From the optimized geometries, DKH2 calculations of static electric mean dipole polarizabilities and polarizability anisotropies are also carried out. It is the first time that the polarizabilities of small iridium clusters have been studied. For n≤4, the mean dipole polarizabilities per atom present an odd–even oscillatory behavior,whereas from Ir5 to Ir8, they decrease with the cluster size increasing. The dependence of the polarizability anisotropy on the structure symmetry of the iridium cluster is verified.

关键词： B3PW91 functional all-electron basis sets DKH2 calculations iridium clusters polarizabilities

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Glyphosate Dissipation in Different Soils Under No-Till and Conventional Tillage

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Pedosphere 2019年第6期29卷 773-783页

作者： Elena OKADA joséLuis costa Francisco BEDMAR Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET) Balcarce 7620(Argentina Estación Experimental Agropecuaria Balcarce Instituto Nacional de Tecnología Agropecuaria(INTA)Balcarce 7620(Argentina Facultad de Ciencias Agrarias Universidad Nacional de Mar del PlataBalcarce 7620(Argentina

Glyphosate is the most used herbicide in Argentina, accounting for 62% of the commercialized pesticides on the market. It is used as a weed controller in no-till systems, and it is also applied to various genetically modified crops ( e.g., soybean, corn, and cotton). Although it has a high solubility in water, it tends to adsorb and accumulate in agricultural soils. The main objectives of this work were to compare the dissipation of glyphosate and the accumulation of its metabolite aminomethylphosphonic acid (AMPA) over time in three soils from agricultural areas of Argentina under long-term management with no-till (NT) and conventional tillage (CT) practices. There were no differences in dissipation between NT and CT, indicating that the glyphosate-degrading microflora was not modified by the different tillage managements. Moreover, tillage practices did not alter the general soil properties;therefore, glyphosate bioavailability was not affected by NT or CT practice. Forty percent of the applied glyphosate was degraded within the first three days in all soils, indicating a fast initial dissipation rate. However, the dissipation rate considerably decreased over time, and the degradation kinetics followed a bi-exponential (or two-compartment) kinetic model. No differences were found between tillage practices. Dissipation was not related to the microbial activity measured as soil respiration. The fast decrease in the concentration of glyphosate at the beginning of the dissipation study was not reflected in an increase in the concentration of AMPA. The estimated half-lives for glyphosate ranged between 9 and 38 d. However, glyphosate bioavailability decreases over time, as it is strongly adsorbed to the soil matrix. This increases its residence time, which may lead to its accumulation in agricultural soils.

关键词： Aminomethylphosphonic acid Bioavailability Biodegradation Herbicide Metabolite Pesticide Two-compartment kinetic model

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Clinical and environmental isolates of Burkholderia pseudomallei from Brazil: Genotyping and detection of virulence gene

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Asian Pacific journal of Tropical Medicine 2017年第10期10卷 945-951页

作者： Tereza de jesus Pinheiro Gomes Bandeira Debora de Souza Collares Maia Castelo-Branco Marcos Fabio Gadelha Rocha Rossana de Aguiar Cordeiro Crister jose Ocadaque Manoel de Araújo Neto Paiva Raimunda Samia Nogueira Brilhante jose júlio costa sidrim Specialized Medical Mycology Center Postgraduate Program in Medical Microbiology Federal University of Ceará Christus School of Medicine Postgraduate Program in Veterinary Sciences State University of Ceará Federal Institute of Education Science and Technology of Ceará

Objective: To evaluate the genetic diversity of clinical and environmental isolates of Burkholderia pseudomallei(***) recovered in Ceara, Brazil, and screen these isolates for the presence of type three secretion system virulence ***: Nineteen *** isolates(9 from clinical cases and 10 from soils)were *** amplified polymorphic DNA was performed with primers OPQ-2, OPQ-4 and OPQ-16 to evaluate the genetic diversity, and type three secretion system gene was detected through polymerase chain ***: Random amplified polymorphic DNA showed a genetic relatedness of approximately 50% among the tested *** isolates, which were grouped into two clades, of which the biggest ones comprised 18/19 isolates for primer OPQ-2, and 17/19 isolates for primer *** OPQ-4 grouped the isolates into three clades comprising 1/19, 3/19 and 15/19 ***, type three secretion system gene was detected in all tested ***: This is an effort to type *** strains from Ceara, which is important for better understanding this pathogen, contributing for the epidemiological surveillance of melioidosis in this endemic region.

关键词： Burkholderia pseudomallei Melioidosis RAPD TTSS gene

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The Use of Triancinolone for the Treatment of Keloid Scars

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journal of Cosmetics, Dermatological Sciences and Applications 2024年第3期14卷 270-275页

作者： josé Humberto Cardoso Resende Luís Otávio Torres Anderson Alves Brandão Delma dos Santos Assis Mercadante jessyka Oliveira Barbosa Batista William Felix de Oliveira Pacheco Elisangelo Aparecido costa Da Silva Douglas Henrique Farias de Oliveira Nébia Maria Almeida de Figueiredo UNIFAN-Centro Universitrio Alfredo Nasser Aparecida de Goinia Brazil UNIRIO Rio de Janeiro Brazil

Scars, when in good evolution, result in a smooth, thin and discreet tissue. Keloid scars, however, are a type of abnormal and exacerbated repair response to tissue injury, whether in surgical interventions or in various injuries, which present in a prominent and gross way. In this context, there is an excess of collagen deposition in the tissue repair process, which can lead to the formation of keloids. The diagnosis of the condition presented is made by the medical professional or by the patient himself after the surgical intervention or skin injury. Under this analysis, protruding, rough and bad-looking scars are identified. In addition, we highlight the existence of keloids similar to large tumors, described as jorge Lobo disease. The treatment encompasses massages, compressions, corticosteroids, chemotherapy, collagenase and cryotherapy. At first, we used corticosteroid-based massages, and then we started using compressive dressings until we started intrakeloid infiltrations with injectable triamcinolone. Triamcinolone 10 mg injectable—1/10—in 0.9% saline, with syringe and fixed needle 0.3 mm × 8 mm, intralesional infiltrate, in this context, proved to be effective for its treatment when applied sequentially and linearly. In cases where the medication was applied, there was an improvement after 21 days of application and a definitive improvement 2 months after the injury. In comparison, on the other hand, patients who were not subjected to the application of the medication may improve after 4 months of the injury or worsen compared to the initial case. We have come to the conclusion that this procedure may be one of the chosen ones for the treatment of keloid scars, being one of the most recommended for cases of keloid already installed.

关键词： Keloid Scar Hypertrophy Collagen Affection

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Risk Governance,Assessment,and Economic Impacts

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International journal of Disaster Risk Science 2017年第3期8卷 235-236页

作者： Paula Teves-costa joséManuel Mendes Instituto Dom Luiz Faculdade de Ciências Universidade de Lisboa European Center on Urban Risks (CERU) Center for Social Studies Faculty of Economics University of Coimbra

Due to climate change,growing urbanization,and population migration the social and economic impacts of natural hazards have been increasing *** challenge for risk science,both methodologically and conceptually,is to i... 详细信息

关键词： Risk Governance,Assessment,and Economic Impacts

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