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Molecular Plant 2012年第4期5卷 951-954页

作者： Giuseppe Ortu Raffaella Balestrini Patricia A. Pereira jorg d. becker Helge Kuste Paola Bonfante department of Life Science and Systems Biology University of Torino Italy Istituto per la Protezione delle Piante--CNR Torino Italy Instituto Gulbenkian de Ciencia 2780-156 Oeiras Portugal Institut for Pflanzengenetik Leibniz Universitat Hannover Herrenhauser Str. 2 D-30419 Hannover Germany

dear Editor, Phytohormones are essential regulators of plant develop- ment, but their role in the signaling processes between plants and fungi during arbuscular mycorrhizal （AM） establishment is far from being understood （Ludwig-M011er, 2010）. AM coloni- zation leads to extensive effects on host metabolism, as revealed by transcriptome studies of AM plants （Hogekamp et al., 2011）. Some genes have been specified as an AM core set, since they are mycorrhizal-responsive, irrespective of the identity of the plant,

关键词： AM真菌植物激素相关基因早期阶段调节过程生物合成相互作用赤霉素

The Properties and Applications of MoS_2/Ti Composite Coatings in dry Cutting and Forming

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材料热处理学报 2004年第05B期25卷 894-898页

作者： XiaolingZhang HailinSun d.becker S.Wagner d.G.Teer TeerCoatingsLtd. WestStineHouseBerryHillIndustrialEstateDroitwichWorcestershireUK InstituteforMetalFormingTechnology stuttgartUniversityStuttgartGermany

MoSi/Ti composite coatings (MoST?), have been deposited by a closed-field unbalanced magnetron sputter ion-plating system. The high ion-current densities at low substrate bias voltage of the system ensures that the MoST? coatings are characteristic of dense coherent structures, with high adhesion (critical scratch load above 120 N), high load bearing capacity up to 5 GPa., low friction (0.02-0.1), high wear resistance (less than 4xlO"17 m3/N m) even at a relative humidity of 40%, and high temperature resistance up to 350 °C. The successful applications of MoST? coatings in large- scale productions for dry cutting and forming, with increasing lifetime and productivity are presented.

关键词： MoS2/Ti复合涂层干切割低摩擦力磁控管溅射

质粒dNA物理形态和其它因素对获得可育转基因小麦的影响

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实验生物学报 2001年第3期34卷 183-189页

作者：徐子勤 d.becker H.Lrz 西北大学生物技术省重点实验室西安710069 德国汉堡大学普通植物学研究所汉堡d22609

本工作分析了不同形态质粒dNA和未成熟胚高渗透压处理对基因枪小麦转化体系的适用性。高渗处理对瞬间表达和转基因小麦的再生均有明显的促进作用。轰击之前对质粒dNA进行变性处理导致瞬间表达反应大幅度下降,但稳定转化频率(指从100个... 详细信息

本工作分析了不同形态质粒dNA和未成熟胚高渗透压处理对基因枪小麦转化体系的适用性。高渗处理对瞬间表达和转基因小麦的再生均有明显的促进作用。轰击之前对质粒dNA进行变性处理导致瞬间表达反应大幅度下降,但稳定转化频率(指从100个轰击未成熟胚得到的再生可育转基因植株数)与双链dNA相差不大。使用单链质粒dNA、线性双链质粒dNA和环状双链质粒dNA均可以得到转基因小麦植株。迄今已得到26个不同的转基因冬小麦株系和4个不同的转基因春小麦株系。这些转基因小麦大多数已产生种子,几个春小麦株系已获第二代种子。

关键词：转基因小麦质粒dNA 物理形态稳定转化育性

A Transcriptome Atlas of Physcomitrella patens Provides Insights into the Evolution and development of Land Plants

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Molecular Plant 2016年第2期9卷 205-220页

作者： Carlos Ortiz-Ramirez Marcela Hernandez-Coronado Anna Thamm Bruno Catarino Mingyi Wang Liam dolan Jose A.Feijo jorg d. becker Instituto Gulbenklan de Ciencia Rua da Quinta Grande 6 2780-156 Oeiras Portugal department of Plant Sciences University of Oxford South Parks Road Oxford OX1 3RB UK division of Plant Biology The Samuel Roberts Noble Foundation Ardmore OK 73401 USA department of Cell Biology and Molecular Genetics University of Maryland 0118 BioSclence Research Building College Park MD 20742-5815 USA These authors contributed equally to this article

Identifying the genetic mechanisms that underpin the evolution of new organ and tissue systems is an aim of evolutionary developmental biology. Comparative functional genetic studies between angiosperms and bryophytes can define those genetic changes that were responsible for developmental innovations. Here, we report the generation of a transcriptome atlas covering most phases in the life cycle of the model bryo- phyte Physcomitrella patens, including detailed sporophyte developmental progression. We identified a comprehensive set of sporophyte-specific transcription factors, and found that many of these genes have homologs in angiosperms that function in developmental processes such as flowering and shoot branching. deletion of the PpTCP5 transcription factor results in development of supernumerary sporangia attached to a single seta, suggesting that it negatively regulates branching in the moss sporophyte. Given that TCP genes repress branching in angiosperms, we suggest that this activity is ancient. Finally, compar- ison of P. patens and Arabidopsis thaliana transcriptomes led us to the identification of a conserved core of transcription factors expressed in tip-growing cells. We identified modifications in the expression patterns of these genes that could account for developmental differences between P. patens tip-growing cells and A. thaliana pollen tubes and root hairs.

关键词： land plant evolution Physcomitrella patens transcriptome atlas TCP branching tip growth

通过卡那霉素选择体系再生可育小麦转基因植株

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自然科学进展（国家重点实验室通讯） 2001年第5期11卷 486-491页

作者：徐子勤 d.becker H.Lrz 西北大学生物技术省重点实验室 Institut fur Allgemeine Botanik Universita HamburgHamburg D-22609Germany

使用卡那霉素作为选择剂建立起一套有效的小麦转化体系,预培养3d的未成熟胚在基因枪轰击前后进行高渗处理,轰击后于26℃、黑暗条件培养2周,然后用150mg/L硫酸卡那霉素选择培养2周,再转至含150mg/L硫酸卡那霉素的再生培养基进行光照培养... 详细信息

使用卡那霉素作为选择剂建立起一套有效的小麦转化体系,预培养3d的未成熟胚在基因枪轰击前后进行高渗处理,轰击后于26℃、黑暗条件培养2周,然后用150mg/L硫酸卡那霉素选择培养2周,再转至含150mg/L硫酸卡那霉素的再生培养基进行光照培养。再生植株在温室中长出2～3片新叶后用2.5％硫酸卡那霉素水溶液喷洒;4周内,非转化体完全白化并枯萎,而转基因植株生长正常,用水稻Actinl启动子控制nptⅡ基因,卡那霉素选择体系的平均转化频率达到2.6％,高渗处理明显促进瞬间表达效果和稳定转化频率。目前已得到23个转基因小麦株系,大多数已经产生种子。Southern分析表明这些转基因株系均整合有不同拷贝的nptⅡ基因。

关键词：卡那霉素选择预培养高渗处理可育转基因小麦基因枪

Assessing the sensitivity of Melanoplus frigidus （Orthoptera： Acrididae） to different weather conditions＂ A modeling approach focussing on development times

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Insect Science 2008年第2期15卷 167-178页

作者： Oliver-d. Finch jorg Loffler Roland Pape Carl-von-Ossietzky University of Oldenburg Fk V Institute of Biology and Environmental-Sciences Terrestrial Ecology D-26111 Oldenburg University of Bonn Department of Geography Meckenheimer AIlee 166 D-53115 Bonn Germany

The temperature and soil moisture conditions as well as vegetation patterns were studied to describe the habitat and to model the life cycle of Melanoplusfrigidus, a true alpine grasshopper of the Scandes. In the low alpine belt of the Norwegian Scandes the species colonizes only the warmest microhabitats with maximum soil surface temperatures of 31℃. Vegetation of these habitats consists of shrub-rich heath dominated by Vaccinium myrtillus and Calluna vulgaris. Using continuously measured temperature data, the development times for four different seasons were modeled and related to field observations. The maximum delay of adult molt was estimated to amount to 3 weeks, the delay being determined by the variation in spring temperature conditions between different years. The possibilities of using M. frigidus as an indicator organism of climate change effects on alpine zoo-coenoses of the Scandes are discussed.

关键词： alpine insects climate change indicator life history thermal effective environment

Alpine Activity Patterns of Mitopus morio(Fabricius, 1779) are Induced by Variations in Temperature and Humidity at different Scales in Central Norway

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Journal of Mountain Science 2014年第3期11卷 644-655页

作者： Nils HEIN Roland PAPE Oliver-d. FINCH jorg LOFFLER department of C-eography University of Bonn Meckenheimer AUee 166 D-53115 Bonn Germany Achtern Nordpol 8 D-26180 Rastede Germany

Our research addresses questions about how micro-climate affects activity abundance of a common and widespread harvestman in an alpine ecosystem. Activity patterns of the Harvestman Mitopus morio(Fabricius, 1779) were studied along different alpine gradients in the central Norwegian Scandes. Within a nested design, we surveyed 18 alpine habitats with pitfall traps and microclimatological equipment along oceanic-continental, two elevational, and(fine-scaled) microtopographic gradients. Sites in the oceanic region of the Scandes showed generally higher abundance of M. morio than sites in the continental region. Furthermore, along the elevational gradient, middle-alpine sites showed higher abundances than low-alpine sites. These general patterns are best explained by higher humidity in the oceanic region and in the middlealpine belt. Focusing at a finer scale, i.e. one elevational level within each region, revealed partly opposing activity patterns within relatively short distances. While in the western middle-alpine belt these patterns were best explained by humidityrelated measures but now with higher activity abundance during drier conditions, in the drier eastern middle-alpine belt heat sums rather than humidity were found to be the best explanatoryvariables for the observed patterns. Hence, our results imply a pronounced different reaction of the two populations towards climatic variables that partly even contradict the previously described general pattern. Regardless whether these differences in activity abundance in M. morio are a form of phenotypic plasticity or adaptation, our findings stress the importance of detailed autecological knowledge combined with fine-scaled climatic measurements when aiming at predictions about possible future ecosystem structures and spatiotemporal phenomena. M. morio proves to be an ideal biogeographic model organism for understanding spatio-temporal responses of alpine ecosystems under modified climatic conditions.

关键词： Climate gradients Opiliones Arctic-alpine ecosystems High mountains Scandinavia

构词法对无意识加工的影响——中德数字启动效应的比较研究

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心理科学 1993年第1期16卷 8-14页

作者：马红骊方芸秋 G.Luer U.Laβ d.becker R.Heuermann 华东师范大学心理系 Georg August Universitat GottingenGermany

本文采用客观觉察阈值作为启动刺激呈现时间、以数字(阿拉伯数字)和数词(以中、德两种文字写出的数词)为刺激比较研究了在掩蔽刺激条件下中国与德国被试的启动效应.结果表明:1)中国被试的启动效应显著,在德国被试中未发现显著的启动效应... 详细信息

本文采用客观觉察阈值作为启动刺激呈现时间、以数字(阿拉伯数字)和数词(以中、德两种文字写出的数词)为刺激比较研究了在掩蔽刺激条件下中国与德国被试的启动效应.结果表明:1)中国被试的启动效应显著,在德国被试中未发现显著的启动效应;2)除数字—数字刺激组外,中国被试的启动效应显著地大于德国被试;3)中国被试的数词—数字刺激组比其他三个刺激组产生的启动效应大。我们从无意识加工特征、构词法的差异以及并行—连续加工理论的观点对上述结果分别进行了讨论.

关键词：构词法无意识加工启动效应

Structure and Function of TPC1 Vacuole SV Channel Gains Shape

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Molecular Plant 2018年第6期11卷 764-775页

作者： Rainer Hedrich Thomas d. Mueller dirk becker Irene Marten Institute for Molecular Plant Physiology and Biophysics University of Wurzburg Julius-yon-Sachs Platz 2 97082 Wurzburg Germany

Plants and animals in endosomes operate TPCI/SV-type cation channels. All plants harbor at least one TPC1 gene. Although the encoded SV channel was firstly discovered in the plant vacuole membrane two decades ago, its biological function has remained enigmatic. Recently, the structure of a plant TPC1/SV channel protein was determined. Insights into the 3d topology has now guided site-directed mutation ap- proaches, enabling structure-function analyses of TPC1/SV channels to shed new light on earlier findings. Fou2 plants carrying a hyperactive mutant form of TPC1 develop wounding stress phenotypes. Recent studies with fou2 and mutants that lack functional TPC1 have revealed atypical features in local and long-distance stress signaling, providing new access to the previously mysterious biology of this vacuolar cation channel type in planta.

关键词： Ca^2+ sensors TPC1/SV channel vacuole membrane voltage voltage sensor