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Electrokinetic-mechanism of water and furfural oxidation on pulsed laser-interlaced Cu_(2)O and CoO on nickel foam

Journal of Energy Chemistry 2024年第4期91卷 145-154页

作者： Yewon oh Jayaraman Theerthagiri M.L.Aruna Kumari Ahreum Min Cheol joo Moon Myong Yong Choi Department of Chemistry(BK21 FOUR) Research Institute of Natural SciencesGyeongsang National UniversityJinju 52828Republic of Korea Department of Chemistry The Oxford College of ScienceBengaluruKarnataka 560102India Core-Facility Center for Photochemistry&Nanomaterials Gyeongsang National UniversityJinju 52828Republic of Korea

The electrocatalytic oxidation of biomass-derived furfural(FF)feedstocks into 2-furoic acid(FA)holds immense industrial potential in optics,cosmetics,polymers,and ***,we fabricated Co O/Ni O/nickel foam(NF)and Cu_(2)O/Ni O/NF electrodes via in situ pulsed laser irradiation in liquids(PLIL)for the bifunctional electrocatalysis of oxygen evolution reaction(OER)and furfural oxidation reaction(FOR),*** oxidation of NF surface to NiO and deposition of CoO and/or Cu_(2)O on NF during PLIL offer distinct advantages for enhancing both the OER and ***/NiO/NF electrocatalyst provides a consistently low overpotential of~359 m V(OER)at 10 m A/cm^(2),achieving the maximum FA yield(~16.37 m M)with 61.5%selectivity,79.5%carbon balance,and a remarkable Faradaic efficiency of~90.1%during 2 h of FOR at 1.43 V(*** hydrogen electrode).Mechanistic pathway via in situ electrochemical-Raman spectroscopy on CoO/NiO/NF reveals the involvement of phase transition intermediates(NiOoh and CoOoh)as surface-active centers during electrochemical *** carbonyl carbon in FF is attacked by hydroxyl groups to form unstable hydrates that subsequently undergo further oxidation to yield FA *** method holds promise for large-scale applications,enabling simultaneous production of renewable building materials and fuel.

关键词： Pulsed laser irradiation in liquids Water and furfural oxidation In situ Raman spectroscopy CoO/NiO/nickel foam Cu_(2)O/Nio/nickel foam 2-furoic acid

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Detection of coat protein gene of nervous necrosis virus using loopmediated isothermal amplification

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Asian Pacific Journal of Tropical Medicine 2016年第3期9卷 230-235页

作者： Jinik Hwang Sung-Suk Suh Mirye Park Myung-joo oh Jong-oh Kim Sukchan Lee Taek-Kyun Lee South Sea Environment Research Department Korea Institute of ocean science & technology Korea University of Science and Technology Department of Aqualife Medicine Chonnam National University Department of Genetic Engineering Sungkyunkwan University

Objective:To establish a novel and highly specific loop-mediated isothermal amplification(LAMP) assay for the identification of nervous necrosis virus(NNV) ***:A set of synthesized primers was used to match the sequences of a specific region of the nnr gene from the National Center for Biotechnology Information database,not originating from NNV-infected fish,the efficiency and specificity of LAMP were measured dependent on the concentration of DNA polymerase and the reaction temperature and *** addition,to determine species-specific LAMP primers,cross reactivity testing was applied to the reaction between NVV and other virus families including viral hemorrhagic septicemia virus and marine ***:The optimized LAMP reaction carried out at 64 ℃ for 60 min,and above 4 U Bst DNA *** sensitivity of LAMP for the detection of nnv was thus about 10 times greater than the sensitivity of polymerase chain *** LAMP assay primers were specific for the detection NNV infection in Epinephelus ***:The development of LAMP primers based on genetic information from a public database,not virus-infected samples,may provide a very simple and convenient method to identify viral infection in aquatic organisms.

关键词： Nervous necrosis virus Nodaviridae Polymerase chain reaction Loop-mediated isothermal Amplification

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Ethanolic extract of dandelion(Taraxacum mongolicum) induces estrogenic activity in MCF-7 cells and immature rats

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Chinese Journal of Natural Medicines 2015年第11期13卷 808-814页

作者： Seung Min oh Ha Ryong Kim Yong joo Park Yong Hwa Lee Kyu Hyuck Chung Fusion Technology Laboratory Hoseo University School of Pharmacy Sungkyunkwan University

Plants of the genus Taraxacum, commonly known as dandelions, are used to treat breast cancer in traditional folk medicine. However, their use has mainly been based on empirical findings without sufficient scientific evidence. Therefore, we hypothesized that dandelions would behave as a Selective estrogen receptor modulator （SERM） and be effective as hormone replacement therapy （HRT） in the postmenopausal women. In the present study, in vitro assay systems, including cell proliferation assay, reporter gene assay, and RT-PCR to evaluate the mRNA expression of estrogen-related genes （pS2 and progesterone receptor, PR）, were performed in human breast cancer cells. Dandelion ethanol extract （DEE） significantly increased cell proliferation and estrogen response element （ERE）-driven luciferase activity. DEE significantly induced the expression of estrogen related genes such as pS2 and PR, which was inhibited by tamoxifen at 1 gmol L-1. These results indicated that DEE could induce estrogenic activities mediated by a classical estrogen receptor pathway. In addition, immature rat uterotrophic assay was carried out to identify estrogenic activity of DEE in vivo. The lowest concentration of DEE slightly increased the uterine wet weight, but there was no significant effect with the highest concentration of DEE. The results demonstrate the potential estrogenic activities of DEE, providing scientific evidence supporting their use in traditional medicine.

关键词： Dandelion extract Estrogenic activity MCF-7 cells Immature rat uterotrophic assay

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Reefs in the Early Paleozoic Taebaek Group,Korea:A Review

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Acta Geologica Sinica(English Edition) 2016年第1期90卷 352-367页

作者： LEE Jeong-Hyun HONG Jongsun WOO Jusun oh Jae-Ryong LEE Dong-Jin CHoh Suk-joo Department of Earth and Environmental Sciences Korea University Department of Earth and Planetary Sciences University of Tennessee Division of Polar Earth-System Sciences Korea Polar Research Institute Department of Earth and Environmental Sciences Andong National University

Various early Paleozoic （Cambrian Series 3-Middle Ordovician） reefs are found in the Taebaek Group, eastern Korea, located in the eastern margin of the Sino-Korean Block. They occur in every carbonate-dominant lithostratigraphic unit of the group, but their morphology and composition differ markedly. The Daegi Formation （middle Cambrian： Cambrian Series 3） contains siliceous sponge-Epiphyton reefs formed in a shallow subtidal environment, which is one of the earliest metazoan-bearing microbial reefs after the archaeocyath extinction. The Hwajeol Formation （upper Cambrian： Furongian） encloses sporadic dendrolites consisting of Angulocellularia, which developed in a relatively deep subtidal environment, representing a rare deeper water example. The onset of the Ordovician radiation resulted in the formation of microbialite-Archaeoscyphia-calathiid patch reefs in shallow subtidal deposits of the Lower Ordovician Dumugol Formation. Subsequent late Early Ordovician relative sea-level fall established extensive peritidal environments, forming microbial mats and stromatolites of the Lower-Middle Ordovician Makgol Formation. Ensuing Ordovician radiation resulted in one of the earliest metazoan skeletal reefs of the Middle Ordovician Duwibong Formation, constructed by stromatoporoid Cystostroma and bryozoan Nicholsonella, and developed around shallow shoals. These reefs reflect ongoing evolution and sea-level change during the early Paleozoic, and exemplify a rare glimpse of peri-Gondwanan records of reef evolution, which warrant detailed investigations and comparison with their counterparts in other regions.

关键词： carbonate sedimentology metazoans stromatolites Porifera Cambrian-Ordovician Taebaeksan Basin Korea

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Application of cationic liposomes for delivery of nucleic acids

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Asian Journal of Pharmaceutical Sciences 2013年第2期8卷 72-80页

作者： Gayong Shim Mi-Gyeong Kim joo Yeon Park Yu-Kyoung oh College of Pharmacy Seoul National UniversityDaehak-dongSeoul 151-742South Korea

Nucleic acid-based bioactive substances have recently emerged as a new class of nextgeneration therapeutics, but their development has been limited by their relatively weakdelivery into target cells. Cationic liposomes have been studied as a means to enhance thestability of nucleic acid therapeutics in the bloodstream and improve their cellular *** nucleic acid therapeutics, siRNA and plasmid DNA have been extensively tested fordelivery using cationic liposomes. This review discusses recent progress in the applicationof cationic liposomes for the delivery of nucleic acid therapeutics.

关键词： Cationic lipid Liposomes Delivery Nucleic acid therapeutics siRNA Plasmid DNA

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A MEMS ultrasound stimulation system for modulation of neural circuits with high spatial resolution in vitro

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Microsystems & Nanoengineering 2019年第1期5卷 356-366页

作者： Jungpyo Lee Kyungmin Ko Hyogeun Shin Soo-Jin oh CJustin Lee Namsun Chou Nakwon Choi Min Tack oh Byung Chul Lee Seong Chan Jun Il-joo Cho Center for BioMicrosystems Brain Science InstituteKorea Institute of Science and Technology(KIST)Seoul 02792Republic of Korea School of Mechanical Engineering Yonsei UniversitySeoul 03722Republic of Korea Division of Bio-Medical Science&Technology KIST SchoolKorea University of Science and Technology(UST)Seoul 02792Republic of Korea Center for Neuroscience Brain Science InstituteKorea Institute of Science and Technology(KIST)Seoul 02792Republic of Korea Convergence Research Center for Diagnosis Treatmentand Care System of DementiaKorea Institute of Science and Technology(KIST)Seoul 02792Republic of Korea Center for Glia-Neuron Interaction Korea Institute of Science and Technology(KIST)Seoul 02792Republic of Korea KU-KIST Graduate School of Converging Science and Technology Korea UniversitySeoul 02841Republic of Korea Division of Bio-Medical Science&Technology KIST SchoolKorea University of Science and TechnologyUSTYuseong-guDaejeon 34113Republic of Korea

Neuromodulation by ultrasound has recently received attention due to its noninvasive stimulation capability for treating brain *** there have been several studies related to ultrasonic neuromodulation,these studies have suffered from poor spatial resolution of the ultrasound and low repeatability with a fixed condition caused by conventional and commercialized ultrasound *** addition,the underlying physics and mechanisms of ultrasonic neuromodulation are still *** determine these mechanisms and accurately modulate neural circuits,researchers must have a precisely controllable ultrasound transducer to conduct experiments at the cellular ***,we introduce a new MEMS ultrasound stimulation system for modulating neurons or brain slices with high spatial *** piezoelectric micromachined ultrasonic transducers(pMUTs)with small membranes(submm membranes)generate enough power to stimulate neurons and enable precise modulation of neural *** designed the ultrasound transducer as an array structure to enable localized modulation in the target *** addition,we integrated a cell culture chamber with the system to make it compatible with conventional cell-based experiments,such as in vitro cell cultures and brain *** this work,we successfully demonstrated the functionality of the system by showing that the number of responding cells is proportional to the acoustic intensity of the applied *** also demonstrated localized stimulation capability with high spatial resolution by conducting experiments in which cocultured cells responded only around a working transducer.

关键词： neural stimulation system

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Gyejibokryeong-Hwan improves recovery of injured muscles in mouse model of muscle contusion

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Journal of Traditional Chinese Medicine 2021年第3期41卷 406-413页

作者： Chi Ho Lee Jeong June Choi joo Hye Sim Younmin Shin Seung-joon oh Ji-Hyang Gu Hyun Ju Ha Dong Hwi Jeon Eun-Jung Lee Min-Seok oh Department of Korean Medicine Rehabilitation College of Korean MedicineDaejeon UniversityDaejeon 34520Republic of Korea Laboratory of Molecular Medicine College of Korean MedicineDaejeon UniversityDaejeon 34520Republic of Korea

OBJECTIVE: To investigate the effects of Gyejibokryeong-Hwan(Guizhifuling-wan, GBH) on muscle injury in a mouse model of muscle ***: C57/BL6 mouse biceps femoris muscles were injured using the drop-mass method and injured animals were treated orally with GBH(50,100, or 500 mg/kg) once a day for 7 d. Open field and treadmill running tests were performed to assess functional recovery from muscle injury. The production of pro-inflammatory cytokines was examined by enzyme-linked immunosorbent assay and Western blotting analysis. Expression of the muscle regeneration biomarkers, myoblast determination(Myo D), myogenic factor 5(Myf5), and smooth muscle actin(α-SMA), in the biceps femoris muscle was investigated at the protein and m RNA level by Western blotting and real time-PCR, respectively. Histological analysis was performed using hematoxylin and eosin staining. Finally, myosin heavy chain production was investigated in differentiated C2C12 myoblasts in the presence of ***: GBH treatment markedly improved locomotion and running behavior. GBH significantly inhibited the secretion of monocyte chemoattractant protein-1 into the bloodstream in muscle-contused animals. The levels of Myo D, Myf5, and α-SMA protein and m RNA were significantly up-regulated by GBH in injured muscle tissue. Histological studies suggested that GBH facilitated recovery from muscle damage. However, GBH did not induce the production of myosin heavy chain in ***: Overall, the present study suggested that GBH improves the recovery of the injured muscles in the mouse model of muscle contusion.

关键词： muscles wounds and injuries contusions regeneration muscle skeletal myogenic regulatory factor 5 Gyejibokryeong-Hwan

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Reactive oxygen species-induced activation of Yes-associated protein-1 through the c-Myc pathway is a therapeutic target in hepatocellular carcinoma

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World Journal of Gastroenterology 2020年第42期26卷 6599-6613页

作者： Yuri Cho Min Ji Park Koeun Kim Sun Woong Kim Wonjin Kim Sooyeon oh joo Ho Lee Department of Internal Medicine CHA Gangnam Medical CenterCHA University School of MedicineSeoul 06135South Korea Department of Internal Medicine Chaum Life CenterCHA University School of MedicineSeoul 06062South Korea Department of Internal Medicine CHA Bundang Medical CenterCHA University School of MedicineSeongnam 13496Bundang guSouth Korea

BACKGROUND The Hippo signaling pathway regulates organ size by regulating cell proliferation and apoptosis with terminal effectors including Yes-associated protein-1(YAP-1).Dysregulation in Hippo pathway has been proposed as one of the therapeutic targets in *** levels of reactive oxygen species(ROS)increase during the progression from early to advanced hepatocellular carcinoma(HCC).AIM To study the activation of YAP-1 by ROS-induced damage in HCC and the involved signaling *** The expression of YAP-1 in HCC cells(Huh-7,HepG2,and SNU-761)was quantified using real-time polymerase chain reaction and *** HCC cells were treated with H2O2,which is a major component of ROS in living organisms,and with either YAP-1 small interfering RNA(siRNA)or control *** investigate the role of YAP-1 in HCC cells under oxidative stress,MTS assays were *** was performed to evaluate the signaling pathway responsible for the activation of ***-eight surgically resected frozen HCC tissue samples and 88 nontumor liver tissue samples were used for gene expression *** H2O2 treatment increased the mRNA and protein expression of YAP-1 in HCC cells(Huh-7,HepG2,and SNU-761).Suppression of YAP-1 using siRNA transfection resulted in a significant decrease in tumor proliferation during H2O2 treatment both in vitro and in vivo(both P<0.05).The oncogenic action of YAP-1 occurred via the activation of the c-Myc pathway,leading to the upregulation of components of the unfolded protein response(UPR),including 78-kDa glucoseregulated protein and activating transcription factor-6(ATF-6).The YAP-1 mRNA levels in human HCC tissues were upregulated by 2.6-fold compared with those in nontumor tissues(P<0.05)and were positively correlated with the ATF-6 Levels(Pearson’s coefficient=0.299;P<0.05).CONCLUSION This study shows a novel connection between YAP-1 and the UPR through the c-Myc pathway during oxidative stress

关键词： Hepatocellular carcinoma Yes-associated protein-1 C-Myc Reactive oxygen species Unfolded protein response Activating transcription factor-6

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Bimodal grain structures and tensile properties of a biomedical Co-20Cr-15W-10Ni alloy with different pre-strains

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Rare Metals 2021年第1期40卷 20-30页

作者： Cheng-Lin Li Seong-Woo Choi Jeong Mok oh Jae-Keun Hong Jong-Taek Yeom joo-Hee Kang Qing-Song Mei Chan Hee Park Department of Materials Engineering School of Power and Mechanical EngineeringWuhan UniversityWuhan 430072China Advanced Metals Division.Korea Institute of Materials Science Changwon 51508Republic of Korea Materials Testing&Reliability Division Korea Institute of Materials ScienceChangwon 51508Republic of Korea

The influence of pre-strain on the formation of bimodal grain structures and tensile properties of a Co-20 Cr-15 W-10 Ni alloy was *** bimodal grain structures consist of fine grains(FGs;2-3μm in diameter)and coarse grains(CGs;8-16μm in diameter),which can be manipulated by changing the pre-strain(ε=0.3-0.7)and annealing temperatures(1000-1100℃).High pre-strain applied in the samples can intensify the plasticity heterogeneity through increasing the total dislocation density and the local volumes of high-density *** can essentially result in finer FGs,a higher FG volume fraction,and overall grain refinement in the samples after ***-temperature essentially increases both the size and volume fraction of CGs,leading to an increase in the average grain *** tensile test suggests that the bimodal grain structured samples exhibited both high strength and ductility,yield strengths of621-877 MPa and ultimate tensile strengths of1187-1367 MPa with uniform elongations of 55.0%-71.4%.The superior strength-ductility combination of the samples arises from the specific deformation mechanisms of the bimodal grain *** tensile properties strongly depend on the size ratio and volume fraction of FGs/CGs in addition to the average grain size in the bimodal grain *** grain structures can be modified via changing the pre-strain and annealing temperature.

关键词： Biomaterials Bimodal grain structure Deformation heterogeneity Pinning High strength/ductility

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Choroidal and skin metastases from colorectal cancer

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World Journal of Gastroenterology 2016年第43期22卷 9650-9653页

作者： joo Young Ha Edward Hynseung oh Moon Ki Jung Song Ee Park Ji Tak Kim In Gyu Hwang Department of Internal Medicine Chung-Ang University College of Medicine Department of Internal Medicine Division of Hemato-oncology Chung-Ang University College of Medicine Department of Ophthalmology Chung-Ang University College of Medicine

Choroidal and skin metastasis of colon cancer is rare. In women, the frequency of cutaneous metastasis from colon cancer as the primary lesion in is 9% and skin metastasis occurs in 0.81% of all colorectal cancers. We report a patient with colonic adenocarcinoma who presented with visual disorder in her right eye and scalp pain as her initial symptoms. Contrast-enhance orbital magnetic resonance imaging with fat suppression revealed an infrabulbar mass, and skin biopsy of the posterior parietal scalp confirmed adenocarcinoma. These symptoms were diagnosed as being caused by choroidal and skin metastases of colonic adenocarcinoma. We started palliative chemotherapy with oral capecitabine(1000 mg/m2, twice a day, on days 1-14) every 3 wk, which was effective at shrinking the brain masses and improving the visual disorder. This is the first report that capecitabine is effective at reducing a choroidal and cutaneous metastatic lesion from rightsided colorectal cancer.

关键词： Colon cancer Choroid cancer Skin cancer Capecitabine

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