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Biphenyl- and carvone-induced protein expression patterns in Rhodococcus sp. ACS

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Journal of Agricultural Chemistry and Environment 2013年第3期2卷 65-73页

作者： jong-shik kim Gyeongbuk Institute for Marine Bioindustry Uljin Republic of Korea

Protein expression patterns in the polychlorinated biphenyl (PCB)-degrading Rhodococcussp. strain ACS were examined following growth on two substrates capable of inducing the enantioselective biotransformation of PCBs via different degradation pathways. Eleven inducible proteins were identified by SDS-PAGE and characterized by LC-MS/MS. Four of the peptides, a spore coat protein, an extracellular serine protease, a spoVP, and a molecular chaperonin from Bacillus subtilis, were identified as being unique to biphenyl-induced cells, whereas anextracellular serine protease from B. subtilis was identified as being unique to carvone-induced cells. None of the peptides identified had sequences that corresponded to known dioxygenases or other PCB-degrading enzymes of this Gram- positive bacterium, suggesting that the identified induced proteins may be involved in either PCB degradation or adaptive responses that protect cells from toxicity.

关键词： Polychlorinated Biphenyl (PCB) Carvone Rhodococcus Protein Expression

Size fractionation and microbial community structure of soil aggregates

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Journal of Agricultural Chemistry and Environment 2013年第4期2卷 75-80页

作者： jong-shik kim David E. Crowley Department of Environmental Sciences University of California Riverside USA Gyeongbuk Institute for Marine Bioindustry Uljin Gyeongbuk Republic of Korea

The microbial community structure in various microaggregates in a loamy sand soil was investigated. The microaggregates were separated into outer and inner aggregates using a series of soil washes. Further physical fractionation of inner aggregates was achieved by separation into coarse and fine sand as macroaggregate fractions, coarse and fine silt as microaggregate fractions, and clay. Research on microbial communities and soil microaggregates can aid in our understanding of soil microhabitats and microorganisms in soil structures, with applications that may contribute to increasing crop production and maintaining sustainable agriculture. In order to study the microbial community structure of aggregates, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) was performed using 16S rRNA genes. The PCR-DGGE of the Bacteria Actinomycetes and Archaea showed divergent results between the different aggregate fractions. The results showed that the bacterial community structure was highly similar between bulk soil and clay;the inner aggregate community structure of Actinomycetes was closely related between coarse and fine sand and coarse silt, and the Archaea community structure of outer and inner aggregates was more similar than that of total bacteria or Actinomycetes.

关键词： Soil Aggregate Microbial Community Fractionation

Application of PCR primer sets for detection of <i>Pseudomonas</i>sp. functional genes in the plant rhizosphere

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Journal of Agricultural Chemistry and Environment 2013年第1期2卷 8-15页

作者： jong-shik kim Pauline M. Mele David E. Crowley Department of Environmental Sciences University of California Riverside USA Department of Primary Industries Rutherglen Australia

Plant growth promoting pseudomonads play an important role in disease suppression and there is considerable interest in development of bio-marker genes that can be used to monitor these bacteria in agricultural soils. Here, we report the application ofa PCR primer sets targeting genes encoding the main antibiotic groups. Distribution of the genes was variably distributed across type strains of 28 species with no phylogenetic groupingfor the detected antibioticsgenes, phlD for 2,4-diacetylphloroglucinol (2,4-DAPG) and phzCD for phenazine-1-carboxylic acid or hcnBC for hydrogen cyanide production. Analysis of field soils showed that primer sets for phlD and phzCD detected these genes in a fallowed neutral pH soil following wheat production, but that the copy numbers were below the detection limits in bulk soils having an acidic pH. In contrast, PCR products for the phzCD, pltc and hcnBc genes were detectable in mature root zones following plantingwith wheat. The ability to rapidly characterize populations of antibiotics producers using specific primer sets will improve our ability to assess the impacts of management practices on the functional traits of Pseudomonas spp. populations in agricultural soils.

关键词： PGPR (Plant Growth-Promoting Rhizosphere) Pseudomonas PCR 16S rDNA Plant-Microbe Interactions

The Archaea Community Associated with Lava-Formed Gotjawal Forest Soil in Jeju, Korea

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Journal of Agricultural Chemistry and Environment 2014年第3期3卷 96-102页

作者： jong-shik kim Man-Young Jung Keun Chul Lee Dae-Shin kim Suk-Hyung Ko Jung-Sook Lee Sung-Keun Rhee Department of Microbiology Chungbuk National University Cheongju Republic of Korea Gyeongbuk Institute for Marine Bioindustry Uljin Republic of Korea Korea Research Institute of Bioscience and Biotechnology Daejeon Republic of Korea Research Institute for Hallasan Jeju Republic of Korea

The abundance and diversity of archaeal assemblages were analyzed in soils collected from Gyorae Gotjawal forest, Jeju, Korea. Gotjawal soil refers to soil derived from a lava-formed forest, characterized by high organic matter content, fertility, and poor rocky soil. Using domain-specific primers, archaeal 16S rRNA gene sequences were PCR amplified for clone library construction, and a total of 185 archaeal clones were examined. The archaeal clones were affiliated with the phyla Thaumarchaeota (96.2%) and Euryarchaeota (3.8%). The most abundant thaumarchaeal group (90.3% of the clones) was the group I.1b clade, which includes soil ammonia-oxidizing archaea. The unique characteristics of Gotjawal soil, including basalt morphology, vegetation, and groundwater aquifer penetration, may be reflected in the archaeal community composition. Further study is necessary to understand the unique factors of Gotjawal soils that influence archaeal abundance, composition, and diversity.

关键词： Archaea Gotjawal Soil 16S rRNA Gene Phylogeny

Development and Experimental Evaluation of Strip Shape Prediction Model for Sendzimir Rolling Mills

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Journal of Iron and Steel Research International 2013年第12期20卷 25-32页

作者： SHIN jong-min HAN Seong-ik kim jong-shik School of Mechanical Engineering Pusan National University School of Electrical Engineering Pusan National University

It is difficult to obtain the desired strip shape using Sendzimir rolling mills because small diameter work rolls can be easily deformed by the roiling force. To control the strip shape effectively, it is important to understand the relationship between the behavior of the shape actuator and the variation of the strip shape. A numerical model based on the contact element method was proposed for the prediction of strip shape. In this numerical model, the re- lationships between the actuating forces, the roll deflections, the thickness profiles of the entry and exit sides, and the strip shape were considered. The proposed numerical model for strip shape prediction was evaluated by computer simulation and experiment with respect to various AS-U roll and first intermediate roll positions.

关键词： Sendzimir rollingmill double acting AS-U roll strip shape prediction model contact element method

Early prediction of myocardial viability after acute myocardial infarction by two-dimensional speckle tracking imaging

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Journal of Geriatric Cardiology 2015年第5期12卷 474-481页

作者： jong Shin Woo Tae-Kyung Yu Woo-shik kim Kwon Sam kim Weon kim Division of Cardiology Department of lnternal Medicine Kyung Hee University Hospital Kyung Hee University Seoul South Korea

Background Identifying the transmural extent of myocardial necrosis and the degree of myocardial viability in acute myocardial infarction （AMI） is important clinically. The aim of this study was to assess myocardial viability using two-dimensional speckle tracking imaging （2D-STI） in patients with AMI. Methods 2D-STI was performed at initial presentation, three days, and six months after primary percutaneous coronary intervention （PCI） in 30 patients with AMI, who had a left anterior descending coronary artery （LAD） culprit lesion. In addition, 20 patients who had minimal stenotic lesions （〈 30% stenosis） on coronary angiography were also included in the control group. At six months dobutamine echocardiography was performed for viability assessment in seven segments of the LAD territory. According to the recovery of wall motion abnormality, segments were classified as viable or non-viable. Results A total of 131 segments were viable, and 44 were nonviable. Multivariate analysis revealed significant differences between the viable and nonviable segments in the peak systolic strain, the peak systolic strain rate at initial presentation, and peak systolic strain rate three days after primary PCI. Among these, the initial peak systolic strain rate had the highest predictive value for myocardial viability （hazard ratio： 31.22, P 〈 0.01）. Conclusions 2D-STI is feasible for assessing myocardial viability, and the peak systolic strain rate might be the most reliable predictor of myocardial viability in patients with AMI.

关键词： Acute myocardial infarction Two-dimensional speckle tracking imaging Viable myocardium

Molecular phylogenetic status of Siberian roe deer(Capreolus pygargus) based on mitochondrial cytochrome b from Jeju Island in Korea

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Chinese Science Bulletin 2014年第32期59卷 4283-4288页

作者： Yong-Su Park Baek-Jun kim Woo-Shin Lee jong-Taek kim Tae-Wook kim Hong-shik Oh Jangsu Endangered Species Restoration Team Jangsu CountyJeonbuk 176-7 Republic of Korea Department of Climate and Ecology National Institute of Ecology Seocheon 325-813 Republic of Korea Department of Forest Science Seoul National UniversitySeoul 151-192 Republic of Korea School of Veterinary Medicine Kangwon National UniversityChuncheon Gangwon 200-701 Republic of Korea Department of Science Education Jeju National UniversityJeju 690-750 Republic of Korea

In order to elucidate the genetic diversity and taxonomic status of Siberian roe deer(Capreolus pygargus)from the Korean peninsula,partial sequences of the mitochondrial cytochrome b gene were *** collected 57 roe deer samples from Mainland and Jeju Island between July 2004 and June 2009.A total of 50 sequences(1,070 bp)were obtained,and seven haplotypes were *** haplotypes were found from Mainland,and two were discovered from Jeju *** genetic diversity was lower in the Jeju Island population than in the Mainland *** addition,both the consensus neighbor-joining tree and maximum parsimony tree showed an identical topology between the Mainland and Jeju Island *** on the results,two subspecies of the Korean roe deer were *** roe deer in Jeju Island showed a distinct difference in the cytochrome b gene compared with other Siberian roe deer,being aunique native species inhabiting only Jeju Island in *** Siberian roe deer in Jeju Island can be classified at subspecies level,the new subspecies in Jeju was named Capreolus pygargus jejuensis in this *** this new subspecies,reasonable conservation and management effort is needed in the future.

关键词：线粒体细胞色素b基因济州岛系统发育地位矮鹿韩国狍遗传多样性分子

Gene editing particle system as a therapeutic approach for drug-resistant colorectal cancer

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Nano Research 2020年第6期13卷 1576-1585页

作者： Jee-Yeon Ryu You Jung Choi Eun-Jeong Won Emmanuel Hui Ho-shik kim Young-Seok Cho Tae-jong Yoon Laboratory of Nanopharmacy College of PharmacyResearch Institute of Pharmaceutical Science and Technology(RIPST)Ajou UniversitySuwon16499Republic of Korea Division of Gastroenterology Department of Internal MedicineSeoul St.Mary’s HospitalCollege of MedicineThe Catholic University of KoreaSeoul06591Republic of Korea Moogene Medi Co. Ltd.Seongnam13488Republic of Korea

The epidermal growth factor receptor(EGFR)pathway plays an important role in the progression of colorectal cancer(CRC).Anti-EGFR drugs based on antibodies have been widely used for treating CRC through inducing the cell death ***,the majority of CRC patients will inevitably develop drug-resistance during anti-EGFR drug treatment,which is mainly caused by a point mutation in the KRAS *** developed a nanoliposomal(NL)particle containing the Cas9 protein and a single-guide RNA(sgRNA)complex(Cas9-RNP),for genomic editing of the KRAS *** NL particle is composed of bio-compatible lipid compounds that can effectively encapsulate *** modifying the NL particle to include the appropriate antibody,it can specifically recognize EGFR expressing CRC and effectively deliver the gene-editing *** conditions of NL treatment were optimized using a KRAS mutated CRC in vivo mouse *** with KRAS-mutated CRC showed drug resistance against cetuximab,a therapeutic antibody *** treating the mice with the KRAS gene-editing NL particles,the implanted tumors showed a dramatic decrease in *** results demonstrated that this genomic editing complex has great potential as a therapeutic carrier system for the treatment of drug-resistant cancer caused by a point mutation.

关键词： nanoliposome clustered regularly interspaced short palindromic repeat and associated Cas9 nuclease(CRISPR/Cas9) KRAS mutation drug-resistance colorectal cancer

Compensation of image noise induced by difference in optical characteristics of array-LED chips of LED print head

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Science China(Technological Sciences) 2017年第12期60卷 1819-1830页

作者： kim Wan-Chin PARK Yong-shik kim Sung-Dae LIM Jiseok Department of Automotive Engineering Honam University Gwansan-gu Gwangju 62399 Korea Mangoslab Co. Ltd. Startupeampus 20 Pangyo-ro 289beon-gil Bundang-gu Seongnam-si Gyeonggi-do 13488 Korea S-Printing Solution Co. Ltd. (Samsung Electronics Ltd) Suwon Gyeonggi-do 443-742 Korea School of Mechanical Engineering Yeungnam University Gyeongsan Gyeongbuk 38541 Korea

There are various applied electro-optical devices, which utilize light emitting didoe(LED) chip array for applications to displays and opto-electronic sensors. In those devices, it is the one of the critical technical issues to minimize uncertain fluctuations including optical power and optical density. Due to variation in operating environment of a device, those are not corrected precisely by controlling parameters based on simple relation between parameters and resultant abovementioned ***, there is essential need to correct outputs in real-time based on correction function generated from the consideration on various operation condition. In this article, we introduce an output correction method through reporting real-time image noise reduction in the application to electro-photography with LED print head. In the technology of LED print head, as differences in optical characteristics between each LED cause vertical image noise, it should be corrected in order to obtain images that are comparable or better in quality compared to those produced by the conventional laser scanning method. Even though it seems that the method used to obtain uniform light power from each LED can solve this problem, it does not work well for high-resolution printing. Therefore, a scan method involving correction by a printed and scanned pattern is introduced through this work. The scan method is composed of correction patterns to minimize printing noise by its shape, the correction algorithm to calculate the optimized value and the printing algorithm to control gray levels in real-time precisely. We believe that the developed correction method upgrades the printing quality of the LPH printer better than commercial printers. The developed correction method can also be applied to various application areas that use an array-type light source such as display systems and lighting systems.

关键词： real-time image correction LED print head(LPH) vertical image noise electro-photography temporal correction spatial correction