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Biphenyl- and carvone-induced protein expression patterns in Rhodococcus sp. ACS

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Journal of Agricultural Chemistry and Environment 2013年第3期2卷 65-73页

作者： jong-shik kim Gyeongbuk Institute for Marine Bioindustry Uljin Republic of Korea

Protein expression patterns in the polychlorinated biphenyl (PCB)-degrading Rhodococcussp. strain ACS were examined following growth on two substrates capable of inducing the enantioselective biotransformation of PCBs via different degradation pathways. Eleven inducible proteins were identified by SDS-PAGE and characterized by LC-MS/MS. Four of the peptides, a spore coat protein, an extracellular serine protease, a spoVP, and a molecular chaperonin from Bacillus subtilis, were identified as being unique to biphenyl-induced cells, whereas anextracellular serine protease from B. subtilis was identified as being unique to carvone-induced cells. None of the peptides identified had sequences that corresponded to known dioxygenases or other PCB-degrading enzymes of this Gram- positive bacterium, suggesting that the identified induced proteins may be involved in either PCB degradation or adaptive responses that protect cells from toxicity.

关键词： Polychlorinated Biphenyl (PCB) Carvone Rhodococcus Protein Expression

Size fractionation and microbial community structure of soil aggregates

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Journal of Agricultural Chemistry and Environment 2013年第4期2卷 75-80页

作者： jong-shik kim David E. Crowley Department of Environmental Sciences University of California Riverside USA Gyeongbuk Institute for Marine Bioindustry Uljin Gyeongbuk Republic of Korea

The microbial community structure in various microaggregates in a loamy sand soil was investigated. The microaggregates were separated into outer and inner aggregates using a series of soil washes. Further physical fractionation of inner aggregates was achieved by separation into coarse and fine sand as macroaggregate fractions, coarse and fine silt as microaggregate fractions, and clay. Research on microbial communities and soil microaggregates can aid in our understanding of soil microhabitats and microorganisms in soil structures, with applications that may contribute to increasing crop production and maintaining sustainable agriculture. In order to study the microbial community structure of aggregates, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) was performed using 16S rRNA genes. The PCR-DGGE of the Bacteria Actinomycetes and Archaea showed divergent results between the different aggregate fractions. The results showed that the bacterial community structure was highly similar between bulk soil and clay;the inner aggregate community structure of Actinomycetes was closely related between coarse and fine sand and coarse silt, and the Archaea community structure of outer and inner aggregates was more similar than that of total bacteria or Actinomycetes.

关键词： Soil Aggregate Microbial Community Fractionation

Application of PCR primer sets for detection of <i>Pseudomonas</i>sp. functional genes in the plant rhizosphere

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Journal of Agricultural Chemistry and Environment 2013年第1期2卷 8-15页

作者： jong-shik kim Pauline M. Mele David E. Crowley Department of Environmental Sciences University of California Riverside USA Department of Primary Industries Rutherglen Australia

Plant growth promoting pseudomonads play an important role in disease suppression and there is considerable interest in development of bio-marker genes that can be used to monitor these bacteria in agricultural soils. Here, we report the application ofa PCR primer sets targeting genes encoding the main antibiotic groups. Distribution of the genes was variably distributed across type strains of 28 species with no phylogenetic groupingfor the detected antibioticsgenes, phlD for 2,4-diacetylphloroglucinol (2,4-DAPG) and phzCD for phenazine-1-carboxylic acid or hcnBC for hydrogen cyanide production. Analysis of field soils showed that primer sets for phlD and phzCD detected these genes in a fallowed neutral pH soil following wheat production, but that the copy numbers were below the detection limits in bulk soils having an acidic pH. In contrast, PCR products for the phzCD, pltc and hcnBc genes were detectable in mature root zones following plantingwith wheat. The ability to rapidly characterize populations of antibiotics producers using specific primer sets will improve our ability to assess the impacts of management practices on the functional traits of Pseudomonas spp. populations in agricultural soils.

关键词： PGPR (Plant Growth-Promoting Rhizosphere) Pseudomonas PCR 16S rDNA Plant-Microbe Interactions

The Archaea Community Associated with Lava-Formed Gotjawal Forest Soil in Jeju, Korea

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Journal of Agricultural Chemistry and Environment 2014年第3期3卷 96-102页

作者： jong-shik kim Man-Young Jung Keun Chul Lee Dae-Shin kim Suk-Hyung Ko Jung-Sook Lee Sung-Keun Rhee Department of Microbiology Chungbuk National University Cheongju Republic of Korea Gyeongbuk Institute for Marine Bioindustry Uljin Republic of Korea Korea Research Institute of Bioscience and Biotechnology Daejeon Republic of Korea Research Institute for Hallasan Jeju Republic of Korea

The abundance and diversity of archaeal assemblages were analyzed in soils collected from Gyorae Gotjawal forest, Jeju, Korea. Gotjawal soil refers to soil derived from a lava-formed forest, characterized by high organic matter content, fertility, and poor rocky soil. Using domain-specific primers, archaeal 16S rRNA gene sequences were PCR amplified for clone library construction, and a total of 185 archaeal clones were examined. The archaeal clones were affiliated with the phyla Thaumarchaeota (96.2%) and Euryarchaeota (3.8%). The most abundant thaumarchaeal group (90.3% of the clones) was the group I.1b clade, which includes soil ammonia-oxidizing archaea. The unique characteristics of Gotjawal soil, including basalt morphology, vegetation, and groundwater aquifer penetration, may be reflected in the archaeal community composition. Further study is necessary to understand the unique factors of Gotjawal soils that influence archaeal abundance, composition, and diversity.

关键词： Archaea Gotjawal Soil 16S rRNA Gene Phylogeny

Development and Experimental Evaluation of Strip Shape Prediction Model for Sendzimir Rolling Mills

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Journal of Iron and Steel Research(International) 2013年第12期20卷 25-32页

作者： SHIN jong-min HAN Seong-ik kim jong-shik School of Mechanical Engineering Pusan National University School of Electrical Engineering Pusan National University

It is difficult to obtain the desired strip shape using Sendzimir rolling mills because small diameter work rolls can be easily deformed by the roiling force. To control the strip shape effectively, it is important to understand the relationship between the behavior of the shape actuator and the variation of the strip shape. A numerical model based on the contact element method was proposed for the prediction of strip shape. In this numerical model, the re- lationships between the actuating forces, the roll deflections, the thickness profiles of the entry and exit sides, and the strip shape were considered. The proposed numerical model for strip shape prediction was evaluated by computer simulation and experiment with respect to various AS-U roll and first intermediate roll positions.

关键词： Sendzimir rollingmill double acting AS-U roll strip shape prediction model contact element method

Milk protein-shelled gold nanoparticles with gastrointestinally active absorption for aurotherapy to brain tumor

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Bioactive Materials 2022年第2期7卷 35-48页

作者： Hyung shik kim Seung Jae Lee Dong Yun Lee Department of Bioengineering College of Engineeringand BK FOUR Biopharmaceutical Innovation Leader for Education and Research GroupHanyang UniversitySeoul04763Republic of Korea Institute of Nano Science and Technology Hanyang UniversitySeoul04763Republic of Korea

Orally absorbable gold nanoparticles(AuNP)having cancer ablation therapy is strongly demanded to treat glioblastoma multiforme(GBM)for patients with its highest incidence ***,we develop a milk protein lactoferrin-conjugated AuNP for its oral absorption and targeting to the GBM through the interaction between lactoferrin(Lf)and lactoferrin receptor(LfR)that is highly expressed in the intestine,blood-brain barrier and *** stability and long circulation of AuNP,glutathione and polyethylene glycol(PEG)is introduced,which is called to *** Lf-PEG-AuNP are orally administered to orthotopic GBM-bearing mice,11-fold and 8-fold higher concentrations of AuNP are measured in bloodstreams and GBM in the brain,respectively,compared with ***,orally administered Lf-PEG-AuNP exhibit an outstanding temperature rise in GBM by irradiating laser and significantly reduce tumor ***,we suggest that the Lf-PEG-AuNP can fundamentally target GBM in the brain through oral absorption,and that its efficient photothermal therapy is possible.

关键词： intestine absorption gastrointestinal

Early prediction of myocardial viability after acute myocardial infarction by two-dimensional speckle tracking imaging

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Journal of Geriatric Cardiology 2015年第5期12卷 474-481页

作者： jong Shin Woo Tae-Kyung Yu Woo-shik kim Kwon Sam kim Weon kim Division of Cardiology Department of lnternal Medicine Kyung Hee University Hospital Kyung Hee University Seoul South Korea

BackgroundIdentifying 心肌的坏死和在尖锐心肌的梗塞(AMI ) 的心肌的生存能力的度的 transmural 程度临床上是重要的。这研究的目的是用二维的点缀追踪估计心肌的生存能力在有 ***2D-STI 的病人的成像( 2D-STI )与 AMI 在 30 个病人... 详细信息

BackgroundIdentifying 心肌的坏死和在尖锐心肌的梗塞(AMI ) 的心肌的生存能力的度的 transmural 程度临床上是重要的。这研究的目的是用二维的点缀追踪估计心肌的生存能力在有 ***2D-STI 的病人的成像( 2D-STI )与 AMI 在 30 个病人在主要经皮的冠的干预(一种总线标准)以后在起始的表示，三天，和六个月被执行，谁有左前面的下降冠的动脉(男孩)犯人损害。另外，有最小的 stenotic 损害的 20 个病人(<；30% 狭窄) 也在控制组在冠的 angiography 上被包括。在六月 dobutamine， echocardiography 在 LAD 领土的七个片断为生存能力评价被执行。根据墙运动反常的恢复，片断作为可行被分类或 131 个片断全部的 *** 是可行的，并且 44 是不能生存的。Multivariate 分析在山峰揭示了可行、不能生存的片断之间的重要差别收缩紧张，山峰在起始的表示的收缩紧张率，和山峰在主要一种总线标准以后的收缩紧张率三天。在这些之中，起始的山峰收缩紧张率为心肌的生存能力有最高预兆的价值(危险比率：31.22， P <；0.01 ).Conclusions2D-STI 为估计收缩紧张率可能是的心肌的生存能力，和山峰是可行的在有 AMI 的病人的心肌的生存能力的最可靠的预言者。

关键词：急性心肌梗死早期预测成像技术追踪斑点二维冠状动脉造影峰值应变

Routine screening for classical azoospermia factor deletions of the Y chromosome in azoospermic patients with Klinefelter syndrome

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Asian Journal of Andrology 2007年第6期9卷 815-820页

作者： Jin Ho Choe jong Woo kim Joong shik Lee Ju Tae Seo Department of Urology Cheil General Hospital Kwandong University College of Medicine Seoul 100-380 Korea

Aim： To evaluate the occurrence of classical azoospermia factor （AZF） deletions of the Y chromosome as a routine examination in azoospermic subjects with Klinefelter syndrome （KS）. Methods： Blood samples were collected from 95 azoospermic subjects with KS （91 subjects had a 47,XXY karyotype and four subjects had a mosaic 47,XXY/46, XY karyotype） and a control group of 93 fertile men. The values of testosterone, follicle stimulating hormone （FSH） and luteinizing hormone （LH） were measured. To determine the presence of Y chromosome microdeletions, polymerase chain reaction （PCR） of five sequence-tagged site primers （sY84, sY 129, sY 134, sY254, sY255） spanning the AZF region, was performed on isolated genomic DNA. Results： Y chromosome microdeletions were not found in any of the 95 azoosperrnic subjects with KS. In addition, using similar conditions of PCR, no microdeletions were observed in the 93 fertile men evaluated. The level of FSH in KS subjects was higher than that in fertile men （38.2 ± 10.3 mIU/mL vs. 5.4 ±2.9 mIU/mL, P 〈 0.001） and the testosterone level was lower than that in the control group （1.7 ±0.3 ng/mL vs. 4.3 ± 1.3 ng/mL, P 〈 0.001）. Conclusion： Our data and review of the published literature suggest that classical AZF deletions might not play a role in predisposing genetic background for the phenotype of azoospermic KS subjects with a 47,XXY karyotype. In addition, routine screening for the classical AZF deletions might not be required for these subjects. Further studies including partial AZFc deletions （e.g. gr/gr or b2/b3） are necessary to establish other mechanism underlying severe spermatogenesis impairment in KS.

关键词： Y chromosome chromosome deletion Klinefelter syndrome azoospermia

Nanostructured hafnium-doped strontium oxide film for homeotropic/homogeneous convertible liquid crystal alignment depending on the curing temperature

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International Journal of Smart and Nano Materials 2022年第4期13卷 597-611页

作者： Dong Hyun kim Dong Wook Lee Jin Young Oh jonghoon Won Hae-Chang Jeong Dae-shik Seo IT Nano Electronic Device Laboratory Department of Electrical and Electronic EngineeringYonsei UniversitySeoulRepublic of Korea Department of Smart Manufacturing Engineering Changwon National UniversityChangwonGyeongnamRepublic of Korea Department of Electrical Electronic and Control Engineering Changwon National UniversityChangwonGyeongnamRepublic of Korea

A hafnium strontium oxide(HfSrO)liquid crystal(LC)alignment film was efficiently created through brush coating,and its ability to change the LC alignment direction was confirmed.A brush was applied to HfSrO sol coated on an indium-tin oxide substrate,after which the coating was solidified at var-ious curing *** was confirmed that a directional micro/nanostructure was formed above 280°C due to the shear stresses caused by the movement of the brush *** chemical changes were analyzed by using X-ray photoelectron spectroscopy and contact angle *** the curing temperature increased,the prevalence of oxygen bonds increased and the contact angle decreased,thereby increasing the surface *** anisotropic boundary of the micro-grooves and the van der Waals forces due to an increase in surface energy changed the alignment direction of LC mole-cules from vertical to horizontal,as verified through polarized optical microscopy and pretilt angle ***,the efficiency of the brush-coating method,which dramatically simplifies the LC alignment film process,was *** homeotropic/homogeneous LC alignment property of the HfSrO film produced through brush coating depending on the curing temperature provides an innovative approach for LC alignment.

关键词： Brush coating nanostructure solution process liquid crystal alignment