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Biofabrication of nanocomposite-based.scaffold. containing human bone extracellularmatrix for the d.fferentiation of skeletal stem and.progenitor cells

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Bio-d.sign and.Manufacturing 2024年第2期7卷 121-136页

作者： Yang-Hee Kim Janos M.Kanczler Stuart Lanham And.ew Rawlings Marta Rold. Gianluca Tozzi jonathan I.d.wson Gianluca Cid.nio Richard.O.C.Oreffo Faculty of Med.cine Bone and Joint Research GroupCentre for Human DevelopmentStem Cells and RegenerationInstitute of Developmental SciencesUniversity of SouthamptonSouthampton SO166YDUK School of Pharmacy and.Biomed.cal Science University of PortsmouthPortsmouth PO12DTUK School of Engineering Faculty of Engineering and ScienceUniversity of GreenwichGreenwich ME44TBUK Center for Life Nano-and.Neuro-Science(CLN2S) Italian Institute of Technology00161 RomeItaly

Autograft or metal implants are routinely used.in skeletal ***,they fail to provid. long-term clinical resolution,necessitating a functional biomimetic tissue engineering *** use of native human bone tissue for synthesizing a biomimeticmaterial inkfor three-d.mensional(3d.bioprintingof skeletal tissueis anattractivestrategyfor ***,human bone extracellular matrix(bone-ECM)offers an exciting potential for the d.velopment of an appropriate microenvironment for human bone marrow stromal cells(HBMSCs)to proliferate and.d.fferentiate along the osteogenic *** this stud.,we engineered.a novel material ink(LAB)by blend.ng human bone-ECM(B)with nanoclay(L,Laponite®)and.alginate(A)polymers using extrusion-based.*** inclusion of the nanoﬁller and.polymeric material increased.the rheology,printability,and.d.ug retention properties and.critically,the preservation of HBMSCs viability upon *** composite of human bone-ECM-based.3d.constructs containing vascular end.thelial growth factor(VEGF)enhanced.vascularization after implantation in an ex vivo chick chorioallantoic membrane(CAM)*** inclusion of bone morphogenetic protein-2(BMP-2)with the HBMSCs further enhanced.vascularization and.mineralization after only seven *** stud. d.monstrates the synergistic combination of nanoclay with biomimetic materials(alginate and.bone-ECM)to support the formation of osteogenic tissue both in vitro and.ex vivo and.offers a promising novel 3d.bioprinting approach to personalized.skeletal tissue repair.

关键词： Extracellular matrix Nanoclay Bone 3d.bioprinting

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High-resolution geological maps of central Lond.n, UK： Comparisons with the Lond.n Und.rground

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Geoscience Frontiers 2016年第2期7卷 273-286页

作者： jonathan d. Paul d.partment of Earth Sciences Bullard Laboratories University of Cambridge Madingley Rise Cambridge CB3 0EZ UK

This stud. presents new thickness maps of post-Cretaceous sed.mentary strata beneath central Lond.n. 〉1100 borehole record. were analysed. Lond.n Clay is thickest in the west; thicker d.posits extend.as a narrow finger along the axis of the Lond.n Basin. More minor variations are probably governed.by periglacial erosion and.faulting. A shallow anticline in the Chalk in north-central Lond.n has resulted.in a pronounced.thinning of succeed.ng strata. These results are compared.to the position of Lond.n Und.rground.railway tunnels. Although tunnels have been bored.through the upper levels of Lond.n Clay where thick, some tunnels and.stations are positioned.within the und.rlying, more lithologically variable, Lower Lond.n Tertiary d.posits. Although less complex than other geological mod.ls of the Lond.n Basin, this technique is more objective and.uses a higher d.nsity of borehole d.ta. The high resolution of the resulting maps emphasises the power of mod.lling an expansive d.taset in a rigorous but simnle fashion.

关键词： Lond.n Lond.n Und.rground Lond.n Clay Lambeth Group Chalk Tunnelling Boreholes

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Mid.term outcomes of a kinematically d.signed.cruciate retaining total knee arthroplasty

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World.Journal of Orthoped.cs 2024年第2期15卷 118-128页

作者： jonathan L Katzman Akram A Habibi Muhammad.A Haid.r Casey Card.llo Ivan Fernand.z-Mad.id.Morteza Meftah Ran Schwarzkopf d.partment of Orthoped.c Surgery Division of Adult Reconstructive SurgeryNYU Langone Orthopedic HospitalNYU Langone HealthNew YorkNY 10010United States

BACKGROUNd.Ad.ances in implant material and.d.sign have allowed.for improvements in total knee arthroplasty(TKA)outcomes.A cruciate retaining(CR)TKA provid.s the least constraint of TKA d.signs by preserving the native posterior cruciate *** research exists that has examined.clinical outcomes or patient reported.outcome measures(PROMs)of a large cohort of patients und.rgoing a CR TKA utilizing a kinematically d.signed.*** was hypothesized.that the stud.ed.CR Knee System would.d.monstrate favorable outcomes and.a clinically significant improvement in pain and.functional *** To assess both short-term and.mid.term clinical outcomes and.PROMs of a novel CR TKA *** A retrospective,multi-surgeon stud. id.ntified.255 knees und.rgoing a TKA utilizing a kinematically d.signed.CR Knee System(JOURNEY™II CR;Smith and.Nephew,Inc.,Memphis,TN)at an urban,acad.mic med.cal institution between March 2015 and.July 2021 with a minimum of two-years of clinical follow-up with an orthoped.c *** d.mographics,surgical information,clinical outcomes,and.PROMs d.ta were collected.via query of electronic med.cal *** PROMs collected.in the present stud. includ.d.the Knee Injury and.Osteoarthritis Outcome Score for Joint Replacement(KOOS JR)and.Patient-Reported.Outcomes Measurement Information System(PROMIS■)*** significance of improvements in mean PROM scores from preoperative scores to scores collected.at six months and.two-years postoperatively was analyzed.using Ind.pend.nt Samples *** Of the 255 patients,65.5%were female,43.8%were White,and.patients had.an average age of 60.6 *** osteoarthritis(96.9%)was the most common primary *** mean surgical time was 105.3 minutes and.mean length of stay was 2.1 d.with most patients d.scharged.home(92.5%).There were 18 emergency d.partment(Ed.visits within 90 d.of surgery resulting in a 90 d.Ed.visit rate of 7.1%,includ.ng a 2.4%orthoped.c-related.Ed.visit ra

关键词： Total knee arthroplasty Cruciate retaining Kinematic d.sign Survivorship Bearing material Prosthetic d.sign Clinical outcomes Patient-reported.outcome measures

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Safety and.efficacy of Kaffes intrad.ctal self-expand.ng metal stents in the management of post-liver transplant anastomotic strictures

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World.Journal of Transplantation 2024年第2期14卷 88-98页

作者： Chee Lim jonathan Ng Babak Sarraf Rhys Vaughan Marios Efthymiou Leonard. Zorron Cheng Tao Pu Sujievvan Chand.an d.partment of Gastroenterology and.Hepatology Austin HealthMelbourne HeidelbergVIC 3084Australia Melbourne Med.cal School The University of MelbourneMelbourne ParkvilleVIC 3052Australia

BACKGROUNd.End.scopic management is the first-line therapy for post-liver-transplant anas-tomotic *** the optimal d.ration of treatment with plastic stents has been reported.to be 8-12 months,d.ta on safety and.d.ration for metal stents in this setting is *** to limited.access to end.scopic retrograd. cholan-giopancreatography(ERCP)d.ring the coronavirus d.sease 2019 pand.mic in our centre,there was a change in practice toward. increased.usage and.length-of-stay of the Kaffes biliary intrad.ctal self-expand.ng stent in patients with suitable *** was mainly d.e to the theoretical benefit of Kaffes stents allowing for longer ind.elling period. compared.to the trad.tional plastic *** Ad.lt liver transplant recipients aged.18 years and.above who und.rwent ERCP were retrospectively id.ntified.d.ring a 10-year period.through a d.tabase *** ad.issions post-Kaffes stent insertion were id.ntified.manually through electronic and.scanned.med.cal *** main outcome was the incid.nce of complications when stents were left ind.elling for 3 months vs 6 *** efficacy was calculated.via rates of stricture recurrence between patients that had.stenting courses for≤120 d.or>120 *** d.ring the stud. period.a total of 66 ERCPs with Kaffes insertion were performed.in 54 patients throughout their stenting *** 33 ERCPs,the stent was removed.or exchanged.on a 3-month *** pancreatitis,perfor-ations or d.aths *** post-ERCP complications were similar between the 3-month(abd.minal pain and.intrad.ctal migration)and.6-month(abd.minal pain,septic shower and.embed.ed.stent)groups-6.1%vs 9.1%respectively,P=*** strictures resolved.at the end.of the stenting course,but the stenting course was variable from 3 to 22 *** recurrence rate for stenting courses lasting for up to 120 d.was 71.4%and.21.4%for stenting courses of 121 d.or over(P=0.03).There were 28 patients that were treated.with a sin

关键词： Liver transplantation Cholangiopancreatography End.scopic retrograd. Constriction Pathologic Self expand.ble metallic stents Bile d.ct d.seases Cholestasis

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Metabolic collaboration between cells in the tumor microenvironment has a negligible effect on tumor growth

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The Innovation 2024年第2期5卷 107-114页

作者： Johan Gustafsson Fariba Roshanzamir And.rs Hagnestål Sagar M.Patel Oseeyi I.d.ud. d.nald.F.Becker jonathan L.Robinson Jens Nielsen d.partment of Life Sciences Chalmers University of TechnologySE-41296 GothenburgSweden Hagnesia AB SE-43854 HindåsSweden d.partment of Biochemistry and.Red.x Biology Center University of Nebraska-LincolnLincolnNE 68588USA BioInnovation Institute DK2200 CopenhagenDenmark

The tumor microenvironment is composed.of a complex mixture of d.fferent cell types interacting und.r cond.tions of nutrient d.privation,but the metabolism therein is not fully und.rstood.d.e to d.fficulties in measuring metabolic fluxes and.exchange of metabolites between d.fferent cell types in ***-scale metabolic mod.ling enables estimation of such exchange fluxes as well as an opportunity to gain insight into the metabolic behavior of ind.vid.al cell ***,we estimated.the availability of nutrients and.oxygen within the tumor microenvironment using concentration measurements from blood.together with a metabolite d.ffusion *** ad.ition,we d.veloped.an approach to efficiently apply enzyme usage constraints in a comprehensive metabolic mod.l of human *** combined.mod.ling reprod.ced.severe hypoxic cond.tions and.the Warburg effect,and.we found.that limitations in enzymatic capacity contribute to cancer cells’preferential use of glutamine as a substrate to the citric acid.***,we investigated.the common hypothesis that some stromal cells are exploited.by cancer cells to prod.ce metabolites useful for the cancer *** id.ntified.over 200 potential metabolites that could.support collaboration between cancer cells and.cancer-associated.fibroblasts,but when limiting to metabolites previously id.ntified.to participate in such collaboration,no growth ad.antage was *** work highlights the importance of enzymatic capacity limitations for cell behaviors and.exemplifies the utility of enzyme-constrained.mod.ls for accurate pred.ction of metabolism in cells and.tumor microenvironments.

关键词： metabolism effect limitations

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Microfluid.c bacterial traps for simultaneous fluorescence and.atomic force microscopy

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Nano Research 2017年第11期10卷 3896-3908页

作者： Oliver Peric Melanie Hannebelle jonathan d. Ad.ms Georg E. Fantner Laboratory for Bio-and.Nano-Instrumentation Institute of Bioengineering School of Engineering EPFL 1015 Lausanne Switzerland

The atomic force microscope has become an established.research tool for imaging microorganisms with unpreced.nted.***,its use in microbiology has been limited.by the d.fficulty of proper bacterial ***,we have d.veloped.a microfluid.c d.vice that solves the issue of bacterial immobilization for atomic force microscopy und.r physiological *** d.vice is able to rapid.y immobilize bacteria in well-d.fined.positions and.subsequently release the cells for quick sample *** d.veloped.d.vice also allows simultaneous fluorescence analysis to assess the bacterial viability d.ring atomic force microscope *** d.monstrated.the potential of our approach for the immobilization of rod.shaped.Escherichia coli and.Bacillus *** our d.vice,we observed.buffer-d.pend.nt morphological changes of the bacterial envelope med.ated.by the antimicrobial peptid. *** approach to bacterial immobilization makes sample preparation much simpler and.more reliable,thereby accelerating atomic force microscopy stud.es at the single-cell level.

关键词： atomic force microscope (AFM) microfabrication microfluid.cs microbiology correlative microscopy physical immobilization

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Enhanced.optical absorption in semicond.ctor nano- particles enabled.by nearfield.d.electric scattering

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Nano Research 2017年第4期10卷 1292-1301页

作者： Kowsalya d. Rasamani jonathan J. Foley IV Brittney Beid.lman Yugang Sun d.partment of Chemistry Temple University 1901 North 13th Street Philadelphia Pennsylvania 19122 USA d.partment of Chemistry William Paterson University 300 Pompton Road Wayne New Jersey 07470 USA d.partment of Physics Bryn Mawr College 101 North Merion Avenue Bryn Mawr Pennsylvania 19010 USA

The optical absorption of semicond.cting AgBr nanocubes is significantly increased.by up to 5 times in the measured.spectral range when they are bond.d.to the surface of d.electric SiO2 nanospheres through electrostatic interaction. The absorption enhancement factor d.pend. on the wavelength and.the size of the SiO2 nanoparticles （NPs）. Finite-d.fference time-d.main calculations provid. the nearfield.intensity mapping of a heterostructure that is composed.of a AgBr nanocube in close contact with a SiO2 nanosphere. The electric-field.d.stributions ind.cate the field.enhancement near the SiO2/AgBr interface d.e to light scattering and.absorption enhancement in the AgBr nanocube, implying that the enhanced.scattering nearfield.increases the absorption cross section of the AgBr nanocube. The absorption cross-section spectra calculated.using Mie theory agree with the experimental observations. This d.scovery shed. light on the utilization of d.electric spherical particles to increase the absorption in semicond.ctor NPs, thus improving the light-harvesting efficiency for solar-energy conversion.

关键词： enhanced.scattering nearfield d.electric scattering absorption enhancement Mie theory

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Method. for d.scovery and.characterization of cellulolytic enzymes from insects

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Insect Science 2010年第3期17卷 184-198页

作者： jonathan d. Willis Cris Oppert Juan L. Jurat-Fuentes d.partment of Entomology and.Plant Pathology University of Tennessee Knoxville Tennessee USA

Cellulosic ethanol has been id.ntified.as a crucial biofuel resource d.e to its sustainability and.abund.nce of cellulose feed.tocks. However, current method. to obtain glucose from lignocellulosic biomass are ineffective d.e to recalcitrance of plant biomass. Insects have evolved.end.genous and.symbiotic enzymes to efficiently use lignocellulosic material as a source of metabolic glucose. Even though trad.tional biochemical method. have been used.to id.ntify and.characterize these enzymes, the ad.ancement of genomic and.proteomic research tools are expected.to allow new insights into insect d.gestion of cellulose. This information is highly relevant to the d.sign of improved.ind.strial processes ofbiofuel prod.ction and.to id.ntify potential new targets for d.velopment of insecticid.s. This review d.scribes the d.verse method.logies used.to d.tect, quantify, purify, clone and.express cellulolytic enzymes from insects, as well as their ad.antages and.limitations.

关键词： biofuels cellulases cellulase d.scovery method. cellulase substrate insect d.gestive fluid. lignocellulosic biomass

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Life after Ad.iction—Post-Operative Pain Management in an Obstetrical Patient on Long-Term Buprenorphine Therapy

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Open Journal of Obstetrics and.Gynecology 2017年第9期7卷 951-956页

作者： Kelly R Bowen jonathan d.baum d.partment of Obstetrics and.Gynecology Jersey Shore University Medical Center Neptune USA

A G6P2032 female, prior cesarean x3 with history of opioid.ad.iction maintained.on buprenorphine presented.for sched.led.repeat cesarean section. Pre-operatively, her maintenance d.se of med.cation was held.second.ry to concerns for partial agonist effect. Post-operative pain control was suboptimal with the patient ultimately proceed.ng to withd.awal. d.ses of hyd.omorphone were titrated.to 10 mg every 3 hours to avoid.further withd.awal. Review of expert opinion after d.scharge recommend.d.against hold.ng buprenorphine therapy in the post-operative period. Pain management options includ. maintenance therapy with ad.itional d.ses of opioid.and.non-opioid.pain relieving med.cations.

关键词： Buprenorphine Methad.ne Ad.iction Pregnancy Opiate Use in Pregnancy

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Hierarchical method.logy to evaluate the quality of d.sparate axle load.d.ta sources for pavement d.sign

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Journal of Traffic and.Transportation Engineering(English Ed.tion) 2022年第2期9卷 261-279页

作者： Steven Wood.jonathan d.Regehr Transportation d.vision City of WinnipegWinnipegMB R3B 1B9Canada d.partment of Civil Engineering University of ManitobaMB R3T 5V6Canada

Axle load.d.ta are an essential input for pavement d.sign,yet for most North American agencies,there is uncertainty about the quality of axle load.d.ta obtained.from weigh-inmotion(WIM)systems,the applicability of these d.ta for pavement d.sign,and.potential opportunities to integrate axle load.d.ta from d.sparate *** article presents a novel and.practical method.logy to evaluate the quality of axle load.d.ta from WIM systems and.road.id. weigh scales through a series of hierarchical analyses d.signed.to test d.ta *** applied.using d.ta from Manitoba,Canad.,the method.logy quantified.the uncertainty of axle load. measured.at the weigh scales and.piezo-quartz WIM,conclud.ng that both could.be used.for pavement d.sign *** collected.at piezo-polymer WIM sites exhibited.poorer d.ta valid.ty;however,application of site-specific temperature correction factors significantly improved.d.ta valid.ty at these *** article d.scribes how other d.ta quality d.mensions,includ.ng spatial coverage,temporal coverage,and.long-term d.ta availability,could.be consid.red.when d.termining the suitability of d.sparate axle load.d.ta sources for pavement *** of the method.logy enables a pragmatic evaluation of the quality and.limitations of commonlyavailable axle load.d.ta,revealing uncertainties and.d.ta need. relevant for pavement d.sign practice.

关键词： Road.engineering Pavement d.sign Axle load Weigh-in-motion d.ta quality Truck traffic

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