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An optimized procedure for plant recovery from somatic embryos significantly facilitates the genetic improvement of Vitis

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Horticulture research 2014年第1期1卷 183-189页

作者： Zhijian T Li Kyung-Hee Kim Sadanand A Dhekney jonathan r jasinski Matthew r Creech Dennis J Gray Grape Biotechnology Core Laboratory Mid-Florida Research and Education CenterUniversity of Florida/IFASApopkaFL 32703-8504USA Department of Plant Sciences Sheridan Research and Extension CenterSheridanWY 82801USA

Plant regeneration from grapevine(Vitis spp.)via somatic embryogenesis typically is *** of plants from Vitis rotundifolia Michx.(muscadine grape)is particularly problematic due to extremely low efficiency,including extended culture durations required for embryo–plant *** plant recovery is an obstacle to the selection of improved genetically modified *** embryos(SEs)of *** cultivar Delicious(Del-HS)and Vitis vinifera L cultivar Thompson Seedless(TS)were used to identify culture media and conditions that promoted embryo differentiation and plant conversion;this resulted in a two-step culture *** comparative culture experiments,C2D medium containing 6%sucrose was the most effective,among four distinct formulae tested,for inducing precocious SE germination and cell *** medium,further supplemented with 4 mM 6-benzylaminopurine(C2D4B),was subsequently determined to enhance post-germinative growth of *** medium supplemented with 0.5 mM 1-naphthaleneacetic acid(MSN)was then utilized to stimulate root and shoot growth of germinated *** average of 35%and 80%‘Del-HS’and‘TS’SE,respectively,developed into *** plants developed robust root and shoot systems and exhibited excellent survival following transfer to *** 150 plants of‘Del-HS’were regenerated and established within 2.5 months,which is a dramatic reduction from the 6-to 12-month time period previously ***,88‘TS’plant lines were obtained within the same time ***,seven out of eight Vitis cultivars exhibited significantly increased plant conversion percentages,demonstrating broad application of the two-step culture system to produce the large numbers of independent plant lines needed for selection of desired traits.

关键词： cultivar Vitis culture

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