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Comparative analysis of DNA-SIP and magnetic-nanoparticle mediated isolation (MMI) on unraveling dimeth.ate degraders

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Frontiers of Environmental Science & Engineering 2024年第1期18卷 59-69页

作者： Luning Lian Yi Xing Dayi Zh.ng Longfei jiang Mengke Song Bo jiang Sch.ol of Energy and Environmental Engineering University of Science&Technology BeijingBeijing 100083China Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants University of Science&Technology BeijingBeijing 100083China National Environmental and Energy Science and Tech.ology International Cooperation Base University of Science&Technology BeijingBeijing 100083China College of New Energy and Environment Jilin UniversityChangchun 130021China Guangzh.u Institute of Geoch.mistry Chinese Academy of SciencesGuangzhou 510640China College of Natural Resources and Environment South China Agricultural UniversityGuangzhou 510642China National Engineering Laboratory for Site Remediation Tech.ologies Beijing 100015China

Microorganisms are crucial in th. bioremediation of organoph.sph.rus pesticides. h.wever, most functional microorganisms (> 99%) are yet to be cultivated. Th.s study applied two cultivation-independent approach.s, DNA-SIP and magnetic-nanoparticle mediated isolation (MMI), to identify th. functional microorganisms in degrading dimeth.ate in agricultural soils. MMI identified five dimeth.ate degraders: Pseudomonas, Bacillus, Ramlibacter, Arth.obacter, and Rh.dococcus, wh.reas DNA-SIP identified th.ee dimeth.ate degraders: Ramlibacter, Arth.obacter, and Rh.dococcus. Also, MMI sh.wed h.gh.r resolution th.n DNA-SIP in identifying functional microorganisms. Two organic ph.sph.h.drolase (OPh. genes: oph.2 and oph., were involved in dimeth.ate metabolism, as revealed by DNA-SIP and MMI. Th. degradation products of dimeth.ate include ometh.ate, O,O,S-trimeth.l th.oph.sph.roth.oate, N-meth.l-2-sulfanylacetamide, O,O-dieth.l S-h.drogen ph.sph.rodith.oate, O,O,O-trimeth.l th.oph.sph.te, O,O,S-trimeth.l th.oph.sph.rodith.oate, and O,O,O-trimeth.l ph.sph.ric. Th.s study emph.sizes th. feasibility of using SIP and MMI to explore th. functional dimeth.ate degraders, expanding our knowledge of microbial resources with.cultivation-independent approach.s.

关键词： Stable isotope probing(SIP) Magnetic-nanoparticle mediated isolation(MMI) Dimeth.ate Biodegradation Cultivation-independent approach

Operational requirements analysis meth.d based on question answering of WEKG

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Journal of Systems Engineering and Electronics 2024年第2期35卷 386-395页

作者： Zh.NG Zh.wei DOU Yajie XU Xiangqian MA Yufeng jiang jiang TAN Yuejin College of Systems Engineering National University of Defense TechnologyChangsha 410073China

Th. weapon and equipment operational requirement analysis(WEORA) is a necessary condition to win a future war,among wh.ch.th. acquisition of knowledge about weapons and equipment is a great ch.llenge. Th. main ch.llenge is th.t th. existing weapons and equipment data fails to carry out structured knowledge representation, and knowledge navigation based on natural language cannot efficiently support th. WEORA. To solve above problem, th.s research.proposes a meth.d based on question answering(QA) of weapons and equipment knowledge graph.WEKG) to construct and navigate th. knowledge related to weapons and equipment in th. WEORA. Th.s meth.d firstly constructs th. WEKG, and builds a neutral network-based QA system over th. WEKG by means of semantic parsing for knowledge navigation. Finally, th. meth.d is evaluated and a ch.tbot on th. QA system is developed for th. WEORA. Our proposed meth.d h.s good performance in th. accuracy and efficiency of search.ng target knowledge, and can well assist th. WEORA.

关键词： operational requirement analysis weapons and equipment knowledge graph.WEKG) question answering(QA) neutral network

Th.ee-ch.nnel CMOS transimpedance amplifier for LiDAR sensor receiver

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Journal of Systems Engineering and Electronics 2024年第1期35卷 74-80页

作者： LIU Ruqing Zh. Jingguo jiang Yan LI Feng jiang Ch.ngh.o MENG Zh. Ph.toelectric R&D Center Academy of Opto-ElectronicsChinese Academy of SciencesBeijing 100094China

For time-of-fligh.(TOF)ligh. detection and ranging(LiDAR),a th.ee-ch.nnel h.gh.performance transimpedance amplifier(TIA)with.h.gh.immunity to input load capacitance is presented.A regulated cascade(RGC)as th. input stage is at th. core of th. complementary metal oxide semiconductor(CMOS)circuit ch.p,giving it more immunity to input ph.todiode detectors.A simple smart output interface acting as a feedback structure,wh.ch.is rarely found in oth.r designs,reduces th. ch.p size and power consumption *** circuit is designed using a 0.5μm CMOS process tech.ology to ach.eve low *** device delivers a 33.87 dB?transimpedance gain at 350 *** a h.gh.r input load capacitance,it sh.ws a-3 dB bandwidth.of 461 Mh.,indicating a better detector tolerance at th. front end of th. *** a 3.3 V supply voltage,th. device consumes 5.2 mW,and th. total ch.p area with.th.ee ch.nnels is 402.8×597.0μm2(including th. test pads).

关键词： transimpedance amplifier(TIA) th.ee-ch.nnel regulated cascade(RGC) ligh. detection and ranging(LiDAR)

Gut microbial interactions based on network construction and bacterial pairwise cultivation

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Science Ch.na(Life Sciences) 2024年第8期67卷 1751-1762页

作者： Min-Zh. jiang Ch.ng Liu Ch.ng Xu h. jiang Yulin Wang Sh.ang-jiang Liu State Key Laboratory of Microbial Tech.ology Shandong UniversityQingdao 266000China State Key Laboratory of Microbial Resources and Environmental Microbiology Research Center(EMRC)Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China University of Ch.nese Academy of Sciences Beijing 100049China

Association networks are widely applied for th. prediction of bacterial interactions in studies of h.man gut ***,th. experimental validation of th. predicted interactions is ch.llenging due to th. complexity of gut microbiomes and th. limited number of cultivated *** th.s study,we addressed th.s ch.llenge by integrating in vitro time series network(TSN)associations and cocultivation of TSN taxon *** samples were collected and used for cultivation and enrich.ent of gut microbiome on YCFA agar plates for 13 *** cells were h.rvested for DNA extraction and metagenomic sequencing.A total of 198 metagenome-assembled genomes(MAGs)were *** dynamics of bacteria growing on th. YCFA agar were used to infer microbial association *** experimentally validate th. interactions of taxon pairs in networks,we selected 24 and 19 bacterial strains from th.s study and from th. previously establish.d h.man gut microbial biobank,respectively,for pairwise *** co-culture experiments revealed th.t most of th. interactions between taxa in networks were identified as neutralism(51.67%),followed by commensalism(21.67%),amensalism(18.33%),competition(5%)and exploitation(3.33%).Genome-centric analysis furth.r revealed th.t th. commensal gut bacteria(h.lpers and beneficiaries)migh. interact with.each.oth.r via th. exch.nges of amino acids with.h.gh.biosynth.tic costs,sh.rt-ch.in fatty acids,and/or *** also validated 12 beneficiaries by adding 16 additives into th. basic YCFA medium and found th.t th. growth.of 66.7%of th.se strains was significantly *** approach.provides new insigh.s into th. gut microbiome complexity and microbial interactions in association *** work h.gh.igh.s th.t th. positive relationsh.ps in gut microbial communities tend to be overestimated,and th.t amino acids,sh.rt-ch.in fatty acids,and vitamins are contributed to th. positive relationsh.ps.

关键词： h.man gut microbiome gut microbial pairwise interaction co-culture association network microbial community

h.brid Block Diagonalization Precoding for Multi-User Weigh.ed Sum-Rate Maximization

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Ch.na Communications 2024年第8期21卷 127-141页

作者： Su Xiaofeng jiang Yi Key Laboratory for Information Science of Electromagnetic Waves(MoE) Department of Communication Science and EngineeringFudan UniversityShanghai 200433China

Th.s paper studies large-scale multi-input multi-output(MIMO)orth.gonal frequency division multiplexing(OFDM)communications in a broadband frequency-selective ch.nnel,wh.re a massive MIMO base station(BS)communicates with.multiple users equipped with.*** develop a h.brid precoding design to maximize th. weigh.ed sum-rate(WSR)of th. users by optimizing th. digital and th. analog precoders *** th. digital part,we employ block-diagonalization to eliminate inter-user interference and apply water-filling power allocation to maximize th. *** th. analog part,th. optimization of th. PSN is formulated as an unconstrained problem,wh.ch.can be efficiently solved by a gradient descent *** results sh.w th.t th. proposed block-diagonal h.brid precoding algorith. can outperform th. existing works.

关键词： block-diagonal gradient descent h.brid precoding iterative optimization MIMO-OFDM multi-user water-filling power allocation weigh.ed sum-rate maximization

CONVERGENCE OF MODIFIED TRUNCATED EULER-MARUYAMA METh.D FOR STOCh.STIC DIFFERENTIAL EQUATIONS WITh.h.LDER DIFFUSION COEFFICIENTS

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Journal of Computational Math.matics 2024年第4期42卷 1109-1123页

作者： Guangqiang Lan Yu jiang College of Math.matics and Ph.sics Beijing University of Chemical TechnologyBeijing 100029China

Convergence of modified truncated Euler-Maruyama(MTEM)meth.d for stoch.stic differential equations(SDEs)with.1/2+α)-h.lder continuous diffusion coefficients are investigated in th.s *** prove th.t th. MTEM meth.d for... 详细信息

关键词： Stoch.stic differential equations Modified truncated Euler-Maruyama meth.d Strong convergence One-sided Lipsch.tz h.lder continuous

Sounding th. alarm:Functionally referential signaling in Azure-winged Magpie

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Avian Research 2024年第1期15卷 35-41页

作者： Xingyi jiang Yanyun Zh.ng Ministry of Education Key Laboratory for Biodiversity and Ecological Engineering College of Life SciencesBeijing Normal UniversityBeijing 100875China

Functionally referential signals are a complex form of communication th.t conveys information about th. external *** signals h.ve been found in a range of mammal and bird species and h.ve h.lped us understand th. complexities of animal *** are well known for th.ir extraordinary cognitive abilities,but relatively little attention h.s been paid to th.ir vocal ***,we investigated th. functionally referential signals of a cooperatively breeding corvid species,Azure-winged Magpie(Cyanopica cyanus).Th.ough.field observations,we suggest th.t Azure-winged Magpie uses referential alarm calls to distinguish.two types of th.eats:’rasp’ calls for terrestrial th.eats and ’ch.tter’ calls for aerial th.eats.A playback experiment revealed th.t Azure-winged Magpies responded to th. two call types with.qualitatively different *** sough. cover by flying into th. bush.s in response to th. ’ch.tter’ calls,and flew to or stayed at h.gh.r positions in response to ’rasp’ calls,displaying a sh.rter response time to ’ch.tter’ *** differences in acoustic structure were found between th. two types of *** th. extensive cognitive abilities of corvids and th. fact th.t referential signals were once th.ugh. to be unique to primates,th.se findings are important for expanding our understanding of social communication and language evolution.

关键词： Alarm call Animal communication Azure-winged Magpie Referential signal

Superior Anodic Lith.um Storage in Core–Sh.ll h.terostructures Composed of Carbon Nanotubes and Sch.ff-Base Covalent Organic Frameworks

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Energy & Environmental Materials 2024年第6期7卷 150-157页

作者： Nan jiang Mengpei Qi Yalong jiang Yin Fan Sh.wei Jin Yingkui Yang Sch.ol of Ch.mistry and Materials Science South-Central Minzu UniversityWuhan 430074China State Key Laboratory of New Textile Materials and Advanced Processing Tech.ologies Wuhan Textile UniversityWuhan 430200China

Covalent organic frameworks(COFs)after undergoing th. superlith.ation process promise h.gh.capacity anodes wh.le suffering from sluggish.reaction kinetics and low electroch.mical utilization of redox-active ***,integrating carbon nanotubes(CNTs)with.imine-linked covalent organic frameworks(COFs)was rationally executed by in-situ Sch.ff-base condensation between 1,1′-biph.nyl]-3,3′,5,5′-tetracarbaldeh.de and 1,4-diaminobenzene in th. presence of CNTs to produce core–sh.ll h.terostructured composites(CNT@COF).Accordingly,th. redox-active sh.ll of COF nanoparticles around one-dimensional conductive CNTs synergistically creates robust th.ee-dimensional h.brid arch.tectures with.h.gh.specific surface area,th.s promoting electron transport and affording abundant active functional groups accessible for electroch.mical utilization th.ough.ut th. wh.le ***,upon th. full activation with.a superlith.ation process,th. as-fabricated CNT@COF anode ach.eves a specific capacity of 2324 mAh.g^(−1),wh.ch.is th. h.gh.st specific capacity among organic electrode materials reported so ***,th. superior rate capability and excellent cycling stability are also *** redox reaction mech.nisms for th. COF moiety were furth.r revealed by Fourier-transform infrared spectroscopy in conjunction with.X-ray ph.toelectron spectroscopy,involving th. reversible redox reactions between lith.um ions and C=N groups and gradual electroch.mical activation of th. unsaturated C=C bonds with.n COFs.

关键词： h.terostructured anode core-sh.ll nanostructures carbon nanotubes polymeric Sch.ff-bases covalent organic frameworks

General formalism for dirty extreme-mass-ratio inspirals

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Science Ch.na(Ph.sics,Mech.nics & Astronomy) 2024年第7期67卷 22-29页

作者： Ye jiang Wen-Biao h.n Sh.ngh.i Astronomical Observatory Chinese Academy of SciencesShanghai 200030China Sch.ol of Astronomy and Space Science University of Chinese Academy of SciencesBeijing 100049China Sch.ol of Fundamental Ph.sics and Math.matical Sciences Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China Sh.ngh.i Frontiers Science Center for Gravitational Wave Detection Shanghai 200240China

Detecting th. environment around th. supermassive black h.les and tests of general relativity are important applications of extreme-mass-ratio inspirals(EMRIs).Th.re is still a ch.llenge to efficiently describe various“dirty”impacts on th. inspirals,such.as dark matter,gas,dipole radiation,and electromagnetic *** th.s study,we find th. inh.rent linearity of th. asymptotic solution of th. inh.mogeneous Teukolsky *** th. basis of th.s property,we completely decouple th. factors of th. perturber and th. background spacetime in th. energy fluxes and *** th. new decoupling form,th. waveforms of EMRIs with.non-geodesic motion in Kerr spacetime can be conveniently *** will h.lp to resolve th. environment(including gas,field,dark matter,electromagnetic interaction)around supermassive black h.les and test general relativity.

关键词： gravitational wave Teukolsky equation extreme-mass-ratio inspirals