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Dpen,ng the Effector Protein Toolbox for Plant- Parasitic Cyst Nematode Interactions

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Molecular Plant 2016年第11期9卷 1451-1453页

作者： Sebastian Eves-van den Akker Paul R.J. Birch Division of Plant Sciences College of Life Sciences University of Dundee Dundee DD1 5EH UK The Department of Biological Chemistry John Innes Centre Norwich Research Park Norwich NR4 7UH UK

Some biotrophic plant pathogens have the remarkable ability to alter plant developmental morphology and subceliular archi- tecture. At least three examples of this ability arose indepen- dently within the phylum Nematoda. For one such example, the cyst nematodes, plant cell manipulation is rapid and pro- found; within days the cell cycle of a single cell in the vascular cylinder is arrested at G2, the vacuole reduces in size and frag- ments.

关键词： GIANT-CELLS

Estimating Urban Households’ Willingness-to-Pay for Upland Forest Restoration in Vietnam

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Open Journal of Forestry 2016年第3期6卷 191-198页

作者： Quy van Khuc Mustapha Alhassan john B. Loomis Trung Duc Tran Mark W. Paschke Department of Forest and Rangeland Stewardship Colorado State University Fort Collins CO USA Forestry Economics Research Center Vietnam Academy of Forest Science Hanoi Vietnam Department of Agricultural Economics University of Nebraska-Lincoln Lincoln NE USA Department of Agricultural and Resource Economics Colorado State University Fort Collins CO USA Department of Hydrology Meteorology & Oceanography Hanoi University of Science Hanoi Vietnam

Increased urbanization coupled with increased reliance of urban communities on rural areas for ecosystem service provision is a challenge faced by many nations. The ability of urban households to directly support restoration efforts in surrounding rural regions represents an underappreciated funding stream for ecological restoration. This study explored the willingness of urban households to support forest restoration in Vietnam. We surveyed 211 households (HHs) in the capital city Hanoi, Vietnam. A Maximum Likelihood Estimator (MLE) model allowed us to obtain the parameters of our model and quantify mean Willingness-to-Pay (WTP) for a program of forest restoration in addition to identifying factors influencing the decision of WTP. Generally, over forty percent of the households surveyed are willing to pay for forest restoration and the mean value of WTP is 37,830 VND ($1.73) per household per month. WTP depends on endogenous and exogenous factors including level of education, income, female-to-male ratio in the household, attitude toward payment for monthly electricity consumption, and awareness of payment for environmental service. Our results suggest that urban household’s demand for forest restoration is real, and represents an untapped source of restoration funding. Policy-makers should take actions to apply charges on water bills to turn this potential into reality for restoration projects in Vietnam if the benefits from restoration outweigh the costs based on our findings.

关键词： Willingness-to-Pay Households Forest Restoration U Minh Thuong National Park Vietnam

急性早幼粒细胞白血病中复杂三元易位的细胞遗传学和分子特点(英文)

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北京大学学报（医学版） 2009年第4期41卷 477-479页

作者： Christopher E. FREEMAN Danielle D. MERCER Yi YE john van BRUNT Ⅲ Marilyn M. LI Human Genetics Program Hayward Genetics Center Tulane University Health Sciences Center 1430 Tulane Avenue New Orleans LA Department of Pediatrics Tulane University Health Sciences Center 1430 Tulane Avenue New Orleans LA Tulane Cancer Center Tulane University Health Sciences Center 1430 Tulane Avenue New Orleans LA

The most frequent chromosomal rearrangement reported in acute promyelocytic leukemia (APL) is t(15;17)(q22;q21). The t(15;17) generates the PML/RARA fusion gene that blocks the transcription of genes involved in myeloid cell differentiation. A small number of simple and complex variants of the classical t(15;17) have been reported. We report two complex three-way translocation variants,t(3;17;15)(q27;q21;q22) and t(8;17;15)(q24.3;q12;q22) in which the PML/RARA fusion gene has been created on the derivative 15 chromosomes. Many of these variant translocations are suspected by conventional cytogenetics but need to be confirmed with additional molecular testing. We discuss the importance of supplementing conventional cytogenetic testing with FISH and RT-PCR to accurately diagnose APL variant patients.

关键词：白血病,早幼粒细胞,急性易位,遗传细胞遗传学

Macrophages are the primary effector cells in IL-7-induced arthritis

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Cellular & Molecular Immunology 2020年第7期17卷 728-740页

作者： Seung-jae Kim Huan J.Chang Michael VVolin Sadiq Umar Katrien van Raemdonck Aimee Chevalier Karol Palasiewicz john W.Christman Suncica Volkov Shiva Arami Mehrdad Maz Anjali Mehta Ryan K.Zomorrodi David A.Fox Nadera Sweiss Shiva Shahrara Division of Rheumatology Jesse Brown VAMedical CenterChicagoIL 60612USA Department of Medicine Division of RheumatologyUniversity of Illinois at ChicagoChicagoIL 60612USA Department of Microbiology and Immunology Chicago College of Osteopathic MedicineMidwestern UniversityDowners GroveIL 60515USA Pulmonary Critical Care and Sleep MedicineOhio State University Wexner Medical CenterDavis Heart and Lung Research InstituteColumbusOH 43210USA Division of Allergy Clinical Immunology and RheumatologyThe University of Kansas Medical CenterKansas CityKS 66160USA Division of Rheumatology and Clinical Autoimmunity Center of Excellence University of MichiganAnn ArborMI 481096USA

Synovial macrophages are crucial in the development of joint inflammation and bone damage;however, the pathways that controlmacrophage remodeling in inflammatory M1 cells or bone-eroding osteoclasts are not fully understood. We determined thatelevated IL-7R/CD127 expression is the hallmark of rheumatoid arthritis (RA) M1 macrophages and that these cells are highlyresponsive to interleukin-7 (IL-7)-driven osteoclastogenesis. We established that lipopolysaccharide (LPS), interferon-γ (IFNγ), andtumor necrosis factor-α (TNFα), the classic M1 macrophage mediators, enhance IL-7R expression in RA and murine *** local expression of IL-7 provokes arthritis, predominantly through escalating the number of F480^(+)iNOS^(+) cells rather than CD3^(+)T cells. Ectopic LPS injection stabilizes IL-7-induced arthritis by increasing myeloid IL-7R expression, in part via IFNγ ***, in RAG−/− mice, IL-7-mediated arthritis is suppressed because of the reduction in myeloid IL-7R expression due to the lackof IFNγ. Moreover, the amelioration of IL-7-induced arthritis by anti-TNF therapy is due to a decrease in the number of cells in theunique F480^(+)iNOS^(+)IL-7R^(+)CCL5^(+) subset, with no impact on the F480^(+)Arginase^(+) cell or CD3^(+) T cell frequency. Consistent with thepreclinical findings, the findings of a phase 4 study performed with RA patients following 6 months of anti-TNF therapy revealedthat IL-7R expression was reduced without affecting the levels of IL-7. This study shifts the paradigm by discovering that IL-7-induced arthritis is dependent on F480^(+)iNOS^(+)IL-7R^(+)CCL5^(+) cell function, which activates TH-1 cells to amplify myeloid IL-7Rexpression and disease severity.

关键词： myeloid elevated inflammation

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共生

建筑实践 2014年第3期 63-67+62页

作者：李莹 john Lewis Marshall john van Helvert

阿姆斯特丹的迪默丹堡(Fort Diemerdam)——这道历史上著名的防线最近被列入联合国教科文组织的世界遗产名录。现存的碉堡和要塞警卫室、原先规划的斜坡和据点均得以修复,自然保护计划也已启动。Emma Architecten建筑事务所在这片区域... 详细信息

阿姆斯特丹的迪默丹堡(Fort Diemerdam)——这道历史上著名的防线最近被列入联合国教科文组织的世界遗产名录。现存的碉堡和要塞警卫室、原先规划的斜坡和据点均得以修复,自然保护计划也已启动。Emma Architecten建筑事务所在这片区域内设计了全新的文化中心,使堡垒重焕生机,在这里游客们得以了解阿姆斯特丹防线的历史,同时,庭院区域与公共活动完美融合在一起,可以满足露天剧场,讲座和音乐演奏的需要。

关键词： WRC 阿姆斯特丹建筑师联合国教科文组织设计灵感外立面土方工程

Expanding the genetic landscape of Rett syndrome to include lysine acetyltransferase 6A(KAT6A)

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Journal of Genetics and Genomics 2020年第10期47卷 650-654页

作者： Simranpreet Kaur Nicole J.van Bergen Bruria Ben-Zeev Emanuela Leonardi Tiong Y.Tan David Coman Benjamin Kamien Susan M.White Miya St john Dean Phelan Kristin Rigbye Sze Chern Lim Michelle C.Torres Melanie Marty Elena Savva Teresa Zhao Sean Massey Alessandra Murgia Wendy A.Gold john Christodoulou Brain and Mitochondrial Research Group Murdoch Children's Research InstituteMelbourneAustralia Department of Paediatrics University of MelbourneMelbourneAustralia Paediatric Neurology Institute The Edmond and Lily Safra Paediatric HospitalSheba Medical CenterTel HaShomerIsrael Sackler School of Medicine Tel Aviv UniversityIsrael Molecular Genetics of Neurodevelopment Department of Woman and Child HealthUniversity of PadovaItaly Fondazione Istituto di Ricerca Pediatrica(IRP) Citta della SperanzaPadovaItaly Victorian Clinical Genetics Services Murdoch Children's Research InstituteMelbourneAustralia Department of Paediatrics The Wesley HospitalBrisbaneBrisbaneAustralia Queensland Children's Hospital BrisbaneAustralia School of Medicine University of QueenslandBrisbaneAustralia Genetic Services of Western Australia Western AustraliaAustralia Faculty of Health and Medical Sciences University of Western AustraliaAustralia Speech and Language Murdoch Children's Research InstituteParkvilleVictoriaAustralia Department of Audiology and Speech Pathology University of MelbourneParkvilleVictoriaAustralia The University of Sydney School of Medical Sciences and Discipline of Child and Adolescent HealthFaculty of Medicine and HealthSydneyAustralia Molecular Neurobiology Lab Kids ResearchWestmead Children's HospitalWestmeadSydneyAustralia Kids Neuroscience Centre Kids ResearchChildren's Hospital at WestmeadWestmeadSydneyAustralia

Pathogenic variants in methyl-Cp G protein 2(MECP2;OMIM300005)result in an X-linked,severe,and progressive epigenetic disorder,Rett syndrome(RTT,OMIM:312750),that predominantly affects females(Rett,1966).Using Neul’s revised diagnostic criteria,affected individuals can be clinically classified as classic or atypical RTT(Neul et al.,2010).

关键词： Rett al. landscape

Sphere-forming corneal cells repopulate dystrophic keratoconic stroma:Implications for potential therapy

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World Journal of Stem Cells 2020年第1期12卷 35-54页

作者： Himanshu Wadhwa Salim Ismail Jennifer J McGhee Bert van der Werf Trevor Sherwin Department of Ophthalmology Faculty of Medical and Health SciencesThe University of AucklandAuckland 1023New Zealand Department of Epidemiology and Biostatistics School of Population HealthFaculty of Medical and Health SciencesThe University of AucklandAuckland 1023New Zealand

BACKGROUND Keratoconus is a degenerative corneal disease characterised by aberrant cell behaviour and loss of matrix that can result in vision *** extracted from peripheral corneas can form stem cell-enriched spheres,which have shown the potential to repopulate the normal peripheral corneal stroma in vitro upon sphere implantation but have not been previously studied in keratoconic *** To investigate the therapeutic potential of stem cell-enriched spheres formed from extracted peripheral human corneal cells when introduced to keratoconic *** Stem cell-enriched spheres were formed from extracts of normal cadaveric human peripheral corneal *** spheres were implanted into incisions created in full thickness and onto the surface of 10μm thin sections of keratoconic and normal stromal tissues in *** sections were used to maximise use of limited keratoconic tissue available for *** cells were stained with Calcein-AM and visualised with stereo and fluorescence microscopy to assess survival and behaviours between the time of implantation day 0 and 14 d(D14)from *** cells in implanted tissues were characterised for stem cell and differentiation markers using immunohistochemistry and droplet digital PCR to assess the potential implications of these characteristics in the use of spheres in keratoconus *** Spheres were successfully implanted into full-thickness central corneal tissue and onto the surface of 10μm thin en face tissue *** observable differences were seen in sphere migration,proliferation or differentiation in keratoconic tissue compared to normal between day 0 and *** stained positively with Calcein-AM up to *** migration increased from day 0 to D14,occurring radially in three dimensions from the sphere and in alignment with tissue *** proliferation marker,EdU,was detected at day *** spheres stained positively for putative stem cell markersΔNp63

关键词： Keratoconus Cell culture Immunohistochemistry Quantitative PCR Digital PCR Spheroid Holoclone Neurosphere Regeneration

Interaction specific binding hotspots in Endonuclease colicin-immunity protein complex from MD simulations

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Science China Chemistry 2013年第8期56卷 1143-1151页

作者： YAO XueXia JI ChangGe XIE DaiQian ZHANG john Z.H. School of Chemistry and Chemical Engineering Nanjing University College of Engineering Nanjing Agricultural University State Key Laboratory of Precision Spectroscopy Department of PhysicsEast China Normal University Department of Chemistry New York University

The binding of Endonuclease colicin 9 (E9) by Immunity protein 9 (Im9) was found to involve some hotspots from helix III of Im9 on protein-protein interface that contribute the dominant binding energy to the *** the current work,MD simulations of the WT and three hotspot mutants (D51A,Y54A and Y55A of Im9) of the E9-Im9 complexes were carried out to investigate specific interaction mechanisms of these three hotspot *** changes of binding energy between the WT and mutants of the complex were computed by the MM/PBSA method using a polarized force field and were in excellent agreement with experiment values,verifying that these three residues were indeed hotspots of the binding *** decomposition analysis revealed that binding by D51 to E9 was dominated by electrostatic interaction due to the presence of the carboxyl group of Asp51 which hydrogen bonds to *** binding by hotspots Y54 and Y55,van der Waals interaction from the aromatic side chain of tyrosine provided the dominant *** comparison,calculation by using the standard (nonpolarizable) AMBER99SB force field produced binding energy changes from these mutations in opposite direction to the experimental *** hydrogen bond analysis showed that conformations sampled from MD simulation in the standard AMBER force field were distorted from the native state and they disrupted the inter-protein hydrogen bond network of the protein-protein *** current work further demonstrated that electrostatic polarization plays a critical role in modulating protein-protein binding.

关键词： protein-protein interaction binding hotspot mutation Endonuclease Colicin immunity protein MD simulation

量子点液晶显示屏(LCD):广泛应用于形成不同的色彩

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今日电子 2013年第6期 53-54页

作者： john van Derlofske Art Lathrop 3M公司

在中世纪，为了和大众沟通交流，工匠们发明了华丽的彩绘玻璃窗。其原理很简单：阳光透过巧妙安排的色彩斑谰的玻璃碎片。然而，色彩背后的科学道理就没这么简单丁。

关键词：液晶显示屏色彩量子点应用玻璃碎片玻璃窗阳光