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Digital imaging approaches for phenotyping whole plant nitrogen and phosphorus response in Brachypodium distachyon

Journal of Integrative plant Biology 2014年第8期56卷 781-796页

作者： Richard poiré Vincent Chochois Xavier R.R.Sirault john p.vogel Michelle Watt Robert T.Furbank CSIRO plant Industry High Resolution plant phenomics Centre CSIRO Plant Industry USDA-ARS Western Regional Research Center

This work evaluates the phenotypic response of the model grass （Brachypodium distacbyon （L.） p. Beauv.） to nitrogen and phosphorus nutrition using a combination of imaging techniques and destructive harvest of shoots and roots. Reference line Bd21-3 was grown in pots using 11 phosphorus and 11 nitrogen concentrations to establish a dose-response curve. Shoot biovolume and biomass, root length and biomass, and tissue phosphorus and nitrogen concentrations increased with nutrient concentration. Shoot biovolume, estimated by imaging, was highly correlated with dry weight （R2 〉 0.92） and both biovolume and growth rate responded strongly to nutrient availability. Higher nutrient supply increased nodal root length more than other root types. photochemical efficiency was strongly reduced by low phosphorus concentrations as early as 1 week after germination, suggesting that this measurement may be suitable for high throughput screening of phosphorus response. In contrast, nitrogen concentration had little effect on photochemical efficiency. Changes in biovolume over time were used to compare growth rates of four accessions in response tonitrogen and phosphorus supply. We demonstrate that a time series image-based approach coupled with mathematical modeling provides higher resolution of genotypic response to nutrient supply than traditional destructive techniques and shows promise for high throughput screening and determina- tion of genomic regions associated with superior nutrient use efficiency.

关键词： Nutrients uptake phenomics photosynthesis root shootCitation: poire R, Chochois V, Sirault XRR, vogel Jp, Watt M, Furbank RT(2o14) Digital imaging approaches for phenotyping whole plantnitrogen and phosphorus response in Brachypodium distachyon.

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对二穗短柄草中抗大麦条纹花叶病毒基因的遗传分析

对二穗短柄草中抗大麦条纹花叶病毒基因的遗传分析

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中国植物病理学会2011年学术年会

作者：崔钰 Naxin Huo john p.vogel David F.Garvin Andrew O.Jackson 刘志勇中国农业大学 USDA-ARS Western Regional Research CenterAlbanyCAUSA USDA-ARS-pSRU University of MinnesotaSt.PaulMNUSA University of California BerkeleyCAUSA.

＜正＞短柄草（Brachypodium distachyon）是一种广泛分布于温带地区的禾本科植物,基因组较小（272Mb）、染色体少、DNA重复序列少（大约15%）、植株较矮、生育期较短,与小麦族植物一样具有二倍体、四倍体和六倍体,且不需要严格的生长...

＜正＞短柄草（Brachypodium distachyon）是一种广泛分布于温带地区的禾本科植物,基因组较小（272Mb）、染色体少、DNA重复序列少（大约15%）、植株较矮、生育期较短,与小麦族植物一样具有二倍体、四倍体和六倍体,且不需要严格的生长条件和栽培措施,是大麦、小麦等早熟禾谷类理想的模式植物。随着二穗短柄草自交系Bd21基因组测序的完成,农杆菌遗传转化体系的建立和T-DNA插入突变及EMS化学诱变突

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A Xyloglucan Backbone 6-O-Acetyltransferase from Brachypodium distachyon Modulates Xyloglucan Xylosylation

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Molecular plant 2016年第4期9卷 615-617页

作者： Lifeng Liu Mon Mandy Hsia Murali Dama john vogel Markus pauly Department of plant & Microbial Biology University of California Berkeley CA 94720 USA U.S. Department of Agriculture Western Regional Research Center Albany CA 94710 USA U.S. Department of Energy Joint Genome Institute Walnut Creek CA 94598 USA

Dear Editor,plant cells are surrounded and protected by cell walls, which consist primarily of polymers such as cellulose, hemicelluloses, pectic polysaccharides, and the polyphenol lignin. One of the ma- jor hemicelluloses in the primary cell wall is xyloglucan （XyG）, which consists of a β-1,4-glucan backbone containing xylosyl substituents. XyG attaches and partially covers the surface of cellulose microfibrils and is therefore thought to act as a spacer between microfibrils, reducing cellulose aggregation （pauly et al., 2013）.

关键词： plant cells surrounded

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Tsc2 mutation induces renal tubular cell nonautonomous disease

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Genes & Diseases 2022年第1期9卷 187-200页

作者： prashant Kumar Fahad Zadjali Ying Yao Daniel johnson Brian Siroky Aristotelis Astrinidis peter vogel Kenneth W.Gross john J.Bissler Department of pediatrics University of Tennessee Health Science Center and Le Bonheur Children's HospitalMemphisTN 38103USA Children's Foundation Research Institute(CFRI) Le Bonheur Children's HospitalMemphisTN 38105USA Department of Clinical Biochemistry College of Medicine&Health SciencesSultan Qaboos UniversityMuscat 123Oman Molecular Bioinformatics Center University of Tennessee Health Science CenterMemphisTN 38163USA Department of Veterinary pathology St.Jude Children's Research HospitalMemphisTN 38105USA Department of Molecular and Cellular Biology Roswell Park Comprehensive Cancer CenterBuffaloNY 14263USA pediatric Medicine Department St.Jude Children's Research HospitalMemphisTN 38105USA

TSC renal cystic disease is poorly understood and has no approved *** a new principal cell-targeted murine model of Tsc cystic disease,the renal cystic epithelium is mostly composed of type A intercalated cells with an intact Tsc2 gene confirmed by sequencing,although these cells exhibit a Tsc-mutant disease *** used a newly derived targeted murine model in lineage tracing and extracellular vesicle(EV)characterization experiments and a cell culture model in EV characterization and cellular induction experiments to understand TSC *** lineage tracing experiments,we found principal cells undergo clonal expansion but contribute very few cells to the *** determined that cystic kidneys contain more interstitial EVs than noncystic kidneys,excrete fewer EVs in urine,and contain EVs in cyst ***,the loss of Tsc2 gene in EV-producing cells greatly changes the effect of EVs on renal tubular epithelium,such that the epithelium develops increased secretory and proliferative pathway *** demonstate that the mTORC1 pathway activity is independent form the EV production,and that the EV effects for a single cell line can vary *** cystogenesis involves significant contribution from genetically intact cells conscripted to the mutant phenotype by mutant cell derived EVs.

关键词： Cell nonautonomous trait polycystic kidney disease Renalcystogenesis Tuberous sclerosis complex

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THE CLINICAL USE OF CALCANEAL BMC MEASUREMENT

THE CLINICAL USE OF CALCANEAL BMC MEASUREMENT

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第一届国际骨质疏松和代谢性骨病研讨会

作者： john Max vogel University of California

＜正＞Methods for measuring bone mass in individual bones of the skeleton,as well as the skeleton as a whole,have been modified over the years with an aim toward improving accuracy and precision and their ability to estimate fracture *** techniques revolve around methods which measure the absorption of either one or two essentially monoenergetic *** isotopic sources with fairly discrete energy beams have been used,the development of X-ray sources with considerably greater photon outputs

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硅酸钙、β-磷酸三钙和羟基磷灰石三相陶瓷复合物与骨细胞活性的体外鉴定(英文)

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中国组织工程研究与临床康复 2010年第16期14卷 3033-3040页

作者： Anne Bernhardt Anja Lode Sivadasan Suresh Babu Antje vogel Thomas Hanke Sebastian Thieme Hare Krishna Varma Angela Rosen-Wolff Michael Gelinsky Annie john Institute of Materials Science Max Bergmann Center forBiomaterials Dresden University of Technology Germany Biomedical Technology Wing Sree Chitra Tirunal Institute for Medical Sciences and Technology Thiruvananthapuram India Department of pediatrics University Clinic Carl Gustav Carus Dresden University of Technology Dresden Germany

由于羟基磷灰石陶瓷与骨无机质的化学成份相似,所以10年来研究者对羟基磷灰石陶瓷在骨修复替换中的应用进行了深入的研究。羟基磷灰石因其高生物相容性和骨传导性已被广泛的应用,但羟基磷灰石的再吸收能力差。文章对由硅酸钙、β-磷酸... 详细信息

由于羟基磷灰石陶瓷与骨无机质的化学成份相似,所以10年来研究者对羟基磷灰石陶瓷在骨修复替换中的应用进行了深入的研究。羟基磷灰石因其高生物相容性和骨传导性已被广泛的应用,但羟基磷灰石的再吸收能力差。文章对由硅酸钙、β-磷酸三钙和羟基磷灰石组成的三相陶瓷复合物进行了研究。在三相陶瓷复合物环境下,采用不同比率硅酸钙、β-磷酸三钙和羟基磷灰石对骨髓夹源间充质干细胞进行体外研究。细胞在加入和没有加入成骨细胞的情况下培养28d。采用碱性磷酸酶活性、碱性磷酸酶基因码转录和骨唾液蛋白Ⅱ表达评估成骨分化水平。成骨细胞和破骨细胞在陶瓷复合80%羟基磷灰石材料上完成预实验。将鼠颅盖骨成骨细胞与人单核细胞共同培养以分析其向破骨细胞分化的可能性。结果显示人骨髓间充质干细胞在陶瓷复合物材料上表现出快速黏附和高度增殖率,在所有实验材料上,成骨诱导的骨髓间充质干细胞碱性磷酸酶活性增加,碱性磷酸酶基因码转录和骨唾液酸蛋白Ⅱ的表达在3种材料上同样增加。扫描电镜和聚焦激光扫描显微镜结果证明在陶瓷复合80%羟基磷灰石材料上的人单核细胞向破骨样细胞分化。结果提示制备的三相陶瓷复合材料支持人骨髓间充质干细胞的黏附、增殖和成骨分化,以及人单核细胞与成骨细胞联合培养条件下破骨细胞形成。

关键词：羟基磷灰石陶瓷 β-磷酸三钙复合物材料骨细胞活性人骨髓间充质干细胞细胞碱性磷酸酶活性硅酸体外鉴定

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polymorphisms in NF-κB,pXR,LXR,ppARγ and risk of inflammatory bowel disease

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World Journal of Gastroenterology 2011年第2期17卷 197-206页

作者： Vibeke Andersen Jane Christensen Anja Ernst Bent A Jacobsen Anne Tjφnneland Henrik B Krarup Ulla vogel Department of Medical Viborg Regional Hospital Institute of Cancer Epidemiology Danish Cancer Society Department of Clinical Biochemistry Aarhus University Hospital Department of Medical Gastroenterology Aarhus University Hospital National Food Institute Technical University of Denmark Institute for Science Systems and ModelsUniversity of Roskilde National Research Centre for the Working Environment

AIM:To investigate the contribution of polymorphisms in nuclear receptors to risk of inflammatory bowel disease(IBD) . METHODS:Genotypes of nuclear factor(NF) -κB(NFKB1) NFκB-94ins/del(rs28362491) ;peroxisome proliferatoractivated receptor(ppAR) -γ(ppARγ) ppARγpro12Ala(rs1801282) and C1431T(rs 3856806) ;pregnane X receptor(pXR) (NR1I2) pXR A-24381C(rs1523127) ,C8055T(2276707) ,and A7635G(rs 6785049) ;and liver X receptor(LXR) (NR1H2) LXR T-rs1405655-C and T-rs2695121-C were assessed in a Danish case-control study of 327 Crohn's disease patients,495 ulcerative colitis(UC) patients,and 779 healthy *** ratio(OR) and 95%CI were estimated by logistic regression models. RESULTS:The pXR A7635G variant,the ppARγpro12Ala and LXR T-rs2695121-C homozygous variant genotypes were associated with risk of UC(OR:1.31,95% CI:1.03-1.66,p=0.03,OR:2.30,95%CI:1.04-5.08,p=0.04,and OR:1.41,95%CI:1.00-1.98,p=0.05,respectively) compared to the corresponding homozygous wild-type *** never smokers,pXR A7635G and the LXR T-rs1405655-C and T-rs2695121-C variant genotypes were associated with risk of IBD(OR:1.41,95%CI:1.05-1.91,p=0.02,OR:1.63,95%CI:1.21-2.20,p=0.001,and OR:2.02,95%CI:1.36-2.99,p=0.0005,respectively) compared to the respective homozygous variant *** A7635G(rs6785049) variant genotype was associated with a higher risk of UC diagnosis before the age of 40 years and with a higher risk of extensive disease(OR:1.34,95%CI:1.03-1.75 and OR:2.49,95%CI:1.24-5.03,respectively) . CONCLUSION:Common pXR and LXR polymorphisms may contribute to risk of IBD,especially among never smokers.

关键词： Crohn's disease Genetic susceptibility Single nucleotide polymorphisms Smoking status Transcription factors Ulcerative colitis

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