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Functional Characterization and RNAi-Mediated Suppression Reveals Roles for Hexose Transporters in Sugar Accumulation by Tomato Fruit

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Molecular Plant 2010年第6期3卷 1049-1063页

作者： David W. McCurdy Stephen Dibley Ricky Cahyanegara Antony Martin john W. patrick School of Environmental and Life Sciences University of Newcastle Callaghan NSW 2308 Australia

Hexoses accumulate to high concentrations （-200 mM） in storage parenchyma cells of tomato fruit. Hexoses are sourced from the fruit apoplasm as hydrolysis products of phloem-imported sucrose. Three hexose transporters （LeHT1, LeHT2, LeHT3）, expressed in fruit storage parenchyma ceils, may contribute to hexose uptake by these cells. An analysis of their full-length sequences demonstrated that all three transporters belong to the STP subfamily of monosaccharide transporters that localize to plasma membranes. Heterologous expression of LeHT1 （and previously LeHT2, Gear et al., 2000）, but not LeHT3, rescued a hexose transportimpaired yeast mutant when raised on glucose or fructose as the sole carbon source. Biochemically, LeHT1, similarly to LeHT2, exhibited transport properties consistent with a high- affinity glucose/H^＋ symporter. Significantly, LeHT1 and LeHT2 also functioned as low-affinity fructose/H^＋ symporters with apparent Km values commensurate with those of fruit tissues. A substantial reduction （80-90%） in fruit expression levels of all LeHT genes by RNAi-mediated knockdown caused a 55% decrease in fruit hexose accumulation. In contrast, photoassimilate production by source leaves and phloem transport capacity to fruit were unaffected by transporter knockdown. Collectively, these findings demonstrate that LeHTs play key roles in driving accumulation of hexoses into storage parenchyma cells during tomato fruit development.

关键词： Fruit hexose transporter sink tomato RNAi.

菜豆茎韧皮部卸出的细胞途径以及激素的促进作用(英文)

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植物生理与分子生物学 1990年第2期 115-124页

作者：沈毓渭,john W.patrick 浙江农业大学原子能农业科学研究所澳大利亚尔斯尔大学生物系杭州 310029

通过缩小叶面积和去茎尖改变源库比率,以调节韧皮部卸出的途径,证明了韧皮部卸出的共质体与质外体途径的季节变化,和由对氯高汞苯磺酸所诱发的从质外体向共质体途径的转变,是与光合产物的输入有关。缩小叶面积而降低源库比率,能增加夏... 详细信息

通过缩小叶面积和去茎尖改变源库比率,以调节韧皮部卸出的途径,证明了韧皮部卸出的共质体与质外体途径的季节变化,和由对氯高汞苯磺酸所诱发的从质外体向共质体途径的转变,是与光合产物的输入有关。缩小叶面积而降低源库比率,能增加夏季生长植株茎韧皮部的质外体卸出,但对冬季生长植株无影响。去尖而增加源库比率,则促进共质体卸出。赤霉酸和激动素能促进共质体的横向转运,但对质外体转运无作用。当质外体为主要运输途径时,赤霉酸和激动素开启共质体途径。赤霉酸和激动素刺激光合产物,通过共质体从筛管一伴胞复合体向韧皮部薄壁纽胞输送,并可能在韧皮部薄壁细胞被动扩散到自由空间。由此可进一步说明蔗糖在激素处理部位自由空间的增加。

关键词：菜豆韧皮部卸出细胞途径赤霉酸激动素

Low-cost,simple,and scalable self-assembly of DNA origami nanostructures

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Nano Research 2019年第5期12卷 1207-1215页

作者： patrick D.Halley Randy A.Patton Amjad Chowdhury john C.Byrd Carlos E.Castro Department of Chemical and Biomolecular Engineering The Ohio State UniversityColumbusOH 43210USA Department of Mechanical and Aerospace Engineering The Ohio State UniversityColumbusOH 43210USA Division of Hematology Department of Internal Medicineand OSU Comprehensive Cancer CenterThe Ohio State UniversityColumbusOH 43210USA Biophysics Graduate Program The Ohio State UniversityColumbusOH 43210USA

Despite demonstrating exciting potential for applications such as drug delivery and biosensing,the development of nanodevices for practical applications and broader use in research and education are still hindered by the time,effort,and cost associated with DNA origami *** and robust methods to perform and scale the DNA origami self-assembly process are critical to facilitate broader use and translation to industrial or clinical *** report a simple approach to fold DNA origami nanostructures that is fast,robust,and *** demonstrate fabrication at scales approximately 100-1,500-fold higher than typical *** further demonstrate an approach we termed low-cost efficient annealing (LEAN) self-assembly involving initial heating at 65 ℃ for 10 min,then annealing at 51 ℃ for 2 h,followed by brief quenching at 4 ℃ that leads to effective assembly of a range of DNA origami structures *** contrast to other methods for scaling DNA origami assembly,this approach can be carried out using cheap and widely available equipment (e.g.,hot plates,water baths,and laboratory burners) and uses standard recipes and materials so is readily applied to any existing or new DNA origami *** envision these methods can facilitate device development for commercial applications and facilitate broader use of DNA origami in research and education.

关键词： DNA origami DNA nanotechnology self assembly scalable nanofabrication low cost fabrication nanotechnology education

Recent laser upgrades at Sandia's Z-backlighter facility in order to accommodate new requirements for magnetized liner inertial fusion on the Z-machine

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High Power Laser Science and Engineering 2016年第4期4卷 1-12页

作者： Jens Schwarz patrick Rambo Darrell Armstrong Marius Schollmeier Ian Smith Jonathan Shores Matthias Geissel Mark Kimmel john Porter Sandia National Laboratories

The Z-backlighter laser facility primarily consists of two high energy, high-power laser systems. Z-Beamlet laser(ZBL)(Rambo et al., Appl. Opt. 44, 2421(2005)) is a multi-kJ-class, nanosecond laser operating at 1054 nm which is frequency doubled to 527 nm in order to provide x-ray backlighting of high energy density events on the Z-machine. Z-Petawatt(ZPW)(Schwarz et al., J. Phys.: Conf. Ser. 112, 032020(2008)) is a petawatt-class system operating at 1054 nm delivering up to 500 J in 500 fs for backlighting and various short-pulse laser experiments(see also Figure 10 for a facility overview). With the development of the magnetized liner inertial fusion(MagLIF) concept on the Z-machine, the primary backlighting missions of ZBL and ZPW have been adjusted accordingly. As a result, we have focused our recent efforts on increasing the output energy of ZBL from 2 to 4 kJ at 527 nm by modifying the fiber front end to now include extra bandwidth(for stimulated Brillouin scattering suppression). The MagLIF concept requires a well-defined/behaved beam for interaction with the pressurized fuel. Hence we have made great efforts to implement an adaptive optics system on ZBL and have explored the use of phase plates. We are also exploring concepts to use ZPW as a backlighter for ZBL driven MagLIF experiments. Alternatively, ZPW could be used as an additional fusion fuel pre-heater or as a temporally flexible high energy pre-pulse. All of these concepts require the ability to operate the ZPW in a nanosecond long-pulse mode, in which the beam can co-propagate with ZBL. Some of the proposed modifications are complete and most of them are well on their way.

关键词： adaptive optics high energy lasers MagLIF OPCPA petawatt lasers SBS suppression

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Same soup different taste-how to best manage the future liver remnant-a surgical perspective

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Hepatobiliary Surgery and Nutrition 2021年第5期10卷 734-736页

作者： john R.Bergquist patrick Starlinger Brendan C.Visser Division of Hepatobiliary and Pancreatic Surgery Department of SurgeryStanford UniversityStanfordCAUSA Division of Hepatobiliary and Pancreatic Surgery Department of SurgeryMayo ClinicRochesterMNUSA

A small but measurable percentage of major hepatic resection patients will experience major morbidity or mortality due post-hepatectomy liver failure after major liver ***-operative optimization,in particular pre-operative induction of liver growth to increase the future liver remnant(FLR)volume,has become frequently used in patients considered to be high ***,this process of hepatic augmentation remains incompletely understood,particularly in *** advances in techniques for FLR management have resulted in dramatic expansion of the liver surgeon’s armamentarium.

关键词： operative hepatic mortality

Practical considerations of dietary therapies for epilepsy in adults

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Acta Epileptologica 2021年第1期3卷 101-107页

作者： Neha Kaul john-Paul Nicolo Terence J.O’Brien patrick Kwan Department of Nutrition and Dietetics Alfred Hospital55 Commercial RoadMelbourneVIC 3004Australia Department of Allied Health(Clinical Nutrition) Royal Melbourne HospitalParkvilleAustralia Departments of Neurosciences and Neurology Alfred Hospital and Monash UniversityMelbourneAustralia Departments of Medicine and Neurology Royal Melbourne HospitalUniversity of MelbourneMelbourneAustralia Chongqing Key Laboratory of Neurology The First Affiliated HospitalChongqing Medical UniversityChongqingChina

Despite the increasing number of anti-seizure medications becoming available,the proportion of patients with drug-resistant epilepsy remains *** therapy for epilepsy is well-established practice in paediatric care,but relatively underutilised in ***,international recommendations have been published to guide the treatment of adults receiving dietary therapy for *** review focuses on the specific aspects of care unique to the management of adults receiving dietary therapy for epilepsy,including patient selection,diet composition,initiation,monitoring and cessation of dietary *** emphasise the need for a multidisciplinary team approach with appropriately trained neurologists and dietitians to provide holistic care while the patients are receiving dietary *** research should focus on the optimal diet composition and meeting the psychosocial needs of adults with epilepsy to maximise efficacy and adherence to dietary treatment.

关键词： Ketogenic diet Epilepsy Diet Drug-resistant epilepsy Nutrition

Gas flow in rotary kilns

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Particuology 2010年第6期8卷 613-616页

作者： patrick R.Davies Michael J.S.Norton D.Ian Wilson john F.Davidson David M.Scott Department of Chemical Engineering and Biotechnology University of CambridgePembroke StreetCambridgeCB2 3RAUK

This paper describes an experimental investigation on the flow characteristics within a rotating cylinder containing a rolling bed of sand. The axis of the cylinder was horizontal and there was no axial bulk flow of particles. The velocity field of the gas flowing through the cylinder was measured by hot-wire anemometry. The measurements indicate that the velocity field is asymmetric with respect to a diameter perpendicular to the granular bed. CFD calculations confirm this finding. The gas velocity profiles are crucial in determining heat transfer from gas to solid.

关键词： Rotating cylinderVelocity profile

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Assessment of Knowledge and Practices of Community Health Nurses on Data Quality in the Ho Municipality of Ghana

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Open Journal of Nursing 2022年第6期12卷 428-443页

作者： Fidelis Zumah john Lapah Niyi patrick Freeman Eweh Benjamin Noble Adjei Martin Alhassan Ajuik Emmanuel Amaglo Wisdom Kwami Takramah Livingstone Asem University of Ghana Medical Centre Ltd. Legon Ghana Gushegu District Health Directorate Gushugu Ghana School of Public Health University of Health and Allied Sciences Ho Ghana Police Clinic Ghana Police Service Ho Ghana

Background: High data quality provides correct and up-to-date information which is critical to ensure, not only for the maintenance of health care at an optimal level, but also for the provision of high-quality clinical care, continuing health care, clinical and health service research, and planning and management of health systems. For the attainment of achievable improvements in the health sector, good data is core. Aim/Objective: To assess the level of knowledge and practices of Community Health Nurses on data quality in the Ho municipality, Ghana. Methods: A descriptive cross-sectional study was employed for the study, using a standard Likert scale questionnaire. A census was used to collect 77 Community Health Nurses’ information. The statistical software, Epi-Data 3.1 was used to enter the data and exported to STATA 12.0 for the analyses. Chi-square and logistic analyses were performed to establish associations between categorical variables and a p-value of less than 0.05 at 95% significance interval was considered statistically significant. Results: Out of the 77 Community Health Nurses studied, 49 (63.64%) had good knowledge on data accuracy, 51 (66.23%) out of the 77 Community Health Nurses studied had poor knowledge on data completeness, and 64 (83.12%) had poor knowledge on data timeliness out of the 77 studied. Also, 16 (20.78%) and 33 (42.86%) of the 77 Community Health Nurses responded there was no designated staff for data quality review and no feedback from the health directorate respectively. Out of the 16 health facilities studied for data quality practices, half (8, 50.00%) had missing values on copies of their previous months’ report forms. More so, 10 (62.50%) had no reminders (monthly data submission itineraries) at the facility level. Conclusion: Overall, the general level of knowledge of Community Health Nurses on data quality was poor and their practices for improving data quality at the facility level were woefully inadequate. Therefore, Co

关键词： Community Health Nurses Data Quality Ho Municipality Ghana

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嵌入虚拟机监视器的高性能虚拟网络

仪器仪表学报 2012年第5期33卷 1195-1200页

作者：唐源夏磊崔峥 john Lange Peter Dinda patrick Bridges 李建平电子科技大学计算机科学与工程学院西北大学电子工程与计算机学院新墨西哥大学计算机学院

由虚拟机和虚拟覆盖网相结合而构成的虚拟计算环境,在云计算和绿色计算中发挥非常重要的作用,然而,现有的虚拟计算环境在性能上难以满足高性能分布式计算的要求。设计和实现了一种高性能虚拟网络:VNET/P。基于网络第2层建立虚拟机互连模... 详细信息

由虚拟机和虚拟覆盖网相结合而构成的虚拟计算环境,在云计算和绿色计算中发挥非常重要的作用,然而,现有的虚拟计算环境在性能上难以满足高性能分布式计算的要求。设计和实现了一种高性能虚拟网络:VNET/P。基于网络第2层建立虚拟机互连模型,对一组虚拟机进行抽象,使其位于同一局域网中。与早期的用户层虚拟网络系统不同,VNET/P嵌入于可扩展、高性能的Palacios虚拟机监视器中。试验结果表明,VNET/P具有高带宽的特点,其性能接近于实际硬件的性能。

关键词：虚拟化虚拟机监视器覆盖网络高性能计算

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