检索结果-南通市图书馆

A spiral microfluidic device for rapid sorting,trapping,and long-term live imaging of Caenorhabditis elegans embryos

引用

Microsystems & Nanoengineering 2023年第1期9卷 203-213页

作者： Peng Pan Zhen Qin William Sun Yuxiao Zhou Shaojia Wang Pengfei Song Yong Wang Changhai Ru Xin Wang john calarco Xinyu Liu Department of Mechanical and Industrial Engineering University of Toronto5 King’s College RoadTorontoOntarioM5S 3G8Canada Upper Canada College 200 Lonsdale RoadTorontoOntarioM4V 1W6Canada School of Advanced Technology Xi’an Jiaotong-Liverpool University111 Ren’ai RoadSuzhou215000China School of Electronic and Information Engineering Suzhou University of Science and TechnologySuzhou215009China Department of Mechanical and Aerospace Engineering Jilin UniversityChangchun130012China Department of Cell&Systems Biology University of Toronto25 Harbord StTorontoOntarioM5S 3G5Canada Institute of Biomedical Engineering University of Toronto164 College StreetTorontoOntarioM5S 3G9Canada

Caenorhabditis elegans embryos have been widely used to study cellular processes and developmental regulation at early ***,most existing microfluidic devices focus on the studies of larval or adult worms rather than *** accurately study the real-time dynamics of embryonic development under different conditions,many technical barriers must be overcome;these can include single-embryo sorting and immobilization,precise control of the experimental environment,and long-term live imaging of *** paper reports a spiral microfluidic device for effective sorting,trapping,and long-term live imaging of single *** embryos under precisely controlled experimental *** device successfully sorts embryos from a mixed population of *** at different developmental stages via Dean vortices generated inside a spiral microchannel and traps the sorted embryos at single-cell resolution through hydrodynamic traps on the sidewall of the spiral channel for long-term *** the well-controlled microenvironment inside the microfluidic device,the response of the trapped *** embryos to mechanical and chemical stimulation can be quantitatively *** experimental results show that a gentle hydrodynamic force would induce faster growth of embryos,and embryos developmentally arrested in the high-salinity solution could be rescued by the M9 *** microfluidic device provides new avenues for easy,rapid,high-content screening of *** embryos.

关键词： larval spiral trapping

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：