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Agave sisalana extract induces cell death in Aedes aegypti hemocytes increasing nitric oxide production

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Asian Pacific Journal of Tropical Biomedicine 2016年第5期6卷 396-399页

作者： Louise Helena Guimaraes de oliveira Patricia Alexandria Paiva Silva de Sousa Fabrine Felipe Hilario Gabriel Joventino Nascimento joao Paulo Saraiva Morais Everaldo Paulo de Medeiros Manoel francisco de Sousa Fabiola da Cruz Nunes Biotechnology Center Federal University of Paraiba Embrapa Algodao Brazilian Agricultural Research Corporation

Objective: To investigate the effects of Agave sisalana(A. sisalana) extract on Aedes aegypti(Ae. aegypti) primary cell ***: Cells of Ae. aegypti were exposed to different concentrations of A. sisalana crude extract(0.18–6.00 mg/m L) for 24 h. Then, the cells were labeled with propidium iodide and subjected to fluorescence microscopy to verify cell viability. In addition, nitric oxide production was ***: Results showed that cells exposed to 6 mg/m L of the crude extract presented a greater percentage of death when compared to control(73.8% ± 9.6% vs. 34.6% ± 9.6%).Furthermore, there was an increase in the nitric oxide production in cells exposed to 6 mg/m L of A. sisalana crude extract [(0.81 ± 0.08) mmol/L] compared to control group[(0.41 ± 0.18) mmol/L].Conclusions: The results show that A. sisalana is cytotoxic to Ae. aegypti and may be used as raw material for new eco-friendly and inexpensive insecticides, since sisal industry discards the liquid waste for the extraction of plant fiber.

关键词： Sisal Cell culture Mosquito Insecticide

Water Quality of Aroeira River for Use in Irrigation of Vegetable Crops in the Northern Region of the State of Espirito Santo, Brazil

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Journal of Agricultural Science and Technology(A) 2014年第10期4卷 797-805页

作者： joao Victor Martins antunes Robson Bonomo Maria da Penha Piccolo Ramos Marcelo Antonio oliveira Institute of Biology State University of Campinas Campinas SP CEP 13083970 Brazil department of Agricultural and Biological Sciences Federal University of Espirito Santo S6o Mateus ES CEP 29932540 Brazil department of Health Sciences Federal University of Espirito Santo S6o Mateus ES CEP 29932540 Brazil

The aim of this study was to evaluate the physical, chemical and microbiological quality of the water of the Aroeira River in the city of Silo Mateus, northern region of Espirito Santo State, Brazil, from April 2012 to March 2013, for use in irrigation of vegetable crops. Five points were selected along the river for analysis of pH, chloride （mg/L C1-）, total hardness （mg/L CaCO3）, dissolved oxygen （mg/L O2）, ammoniacal nitrogen （mg/L N-NH3）, total iron （mg/L Fe）, phosphate/orthophosphate （mg/L PO43-）, turbidity （NTU）, acidity （mg/L CO2）, temperature （℃）, electrical conductivity （dS/m）, as well as microbiological and parasitological analyses. Only point l, located upstream of the points of domestic sewage discharges, is the closest to be considered suitable for use in irrigation of vegetable crop, demonstrating the possible risks to the health of populations who use water with sewage discharges in urban communities for irrigation of vegetable crops.

关键词： Contamination agriculture parasitic diseases coliforms sewage.

Adenoma incidence decreases u nder the efect of polypectomy

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World Journal of Gastroenterology 2012年第11期18卷 1243-1248页

作者： Isadora Rosa Paulo Fidalgo Jose Soares Susana Vinga Carla oliveira joao P Silva Susana M Ferro Paula Chaves Antonio G oliveira Carlos N Leitao Gastroenterology department Instituto Portu-gues de Oncologia de Lisboa Francisco Gentil EPE 1099-023Lisboa Portugal Faculdade de Ciencias da Safldeda Universidade da Beira Interior 6200-506 Covilha Portugal Jose Soares Gastroenterology Department Hospital de SantoAntonio EPE 4099-001 Porto Portugal Instituto de Engenharia de Sistemas e Com-putadores: Investigacao e desenvolvimento 1000-029 LisboaPortugal Hospital de Santo Antonio EPE 4099-001Porto Portugal Pathology department Instituto Portugues deOncologia de Lisboa Francisco Gentil EPE 1099-023 LisboaPortugal Biostatistics department Faculdade deCiencias M6dicas 1169-056 Lisboa Portugal

MM： To investigate whether, under the influence of pol ypectomy, the incidence of adenoma decreases with age.METHODS： Consecutive patients with colonic adenomas identified at index colonoscopy were retrospectively selected if they had undergone three or more complete colonoscopies, at least 24 mo apart. Patients who had any first-degree relative with colorectal cancer were excluded. Data regarding number of adenomas at each colonoscopy, their location, size and histological classification were recorded. The monthly incidence density of adenomas after the index examination was estimated for the study population, by using the person-years method. Baseline adenomas were excluded from incidence calculations but their characteristics were correlated with recurrence at follow-up, using the X^42 test.RESULTS： One hundred and fifty-six patients were included （109 male, mean age at index colonoscopy 56.8 ± 10.3 years）, with follow-up that ranged from 48 to 232 mo. No significant correlations were observed between the number, the presence of villous component, or the size of adenomas at index colonoscopy and the presence of adenomas at subsequent colonoscopies （P = 0.49, 0.12 and 0.78, respectively）. The incidence of colonic adenomas was observed to decay from 1.4% person-months at the beginning of the study to values close to 0%, at 12 years after index colonoscopy.CONCLUSION： Our results suggest the sporadic formation of adenomas occurs within a discrete period and that, when these adenomas are removed, all neoplasia-prone clones may be extinguished.

关键词： Colorectal cancer Colorectal adenoma In-cidence Age Polypectomy

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吉马良斯私人医院

城市环境设计 2011年第Z3期 208-217页

作者： Marlene Sousa joao Couto Fernando Torres francisco oliveira Helio Pinto joao Carvalho Luis Ferreira AIves 刘胜杰毕达哥拉斯(pitagoras)建筑事务所中国中建设计集团有限公司

吉马良斯私人医院位于吉马良斯市政会新建造的具有完备服务设施的吉马良斯市郊.这些综合设施包括运动场、综合游泳池以及一些特殊组织投资建设的中小学。医院基地紧邻Creixomil草场.位于斜坡道之间,极具医院建筑的特色。场地的条件非常... 详细信息

吉马良斯私人医院位于吉马良斯市政会新建造的具有完备服务设施的吉马良斯市郊.这些综合设施包括运动场、综合游泳池以及一些特殊组织投资建设的中小学。医院基地紧邻Creixomil草场.位于斜坡道之间,极具医院建筑的特色。场地的条件非常适合建筑的尺度.建筑由两部分构成.其中一个紧凑的单元有两层.向更远处延伸.两个区域的重叠部分是一个独立的横向单元.用来作为收容区。这样的处理使医院内外的管理简单便捷。天井上的砌块由水平与垂直的循环部分组成.

关键词：医院建筑吉马良斯私人

Public Use and Landscape Analysis in the Serra da Canastra National Park, Brazil: A Geospatial Approach

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Natural Resources 2016年第2期7卷 93-101页

作者： Marcos Cicarini Hott Luiz Otávio Moras Filho Marco Aurélio Leite Fontes Aline Aparecida Silva Pereira Cláudia de oliveira Gonçalves Nogueira Luis Marcelo Tavares de Carvalho Luís Antônio Coimbra Borges João Cesar de Resende Mauro Antonio Homem antunes department of Forest Sciences Federal University of Lavras Lavras Brazil Brazilian Agricultural Research Corporation—Embrapa Juiz de Fora Brazil department of Engineering Federal Rural University of Rio de Janeiro Seropédica Brazil

The Conservation Units (CU) were created to protect natural environments from growing degradation and to impede the expansion of urbanization and agricultural crops. The Serra da Canastra National Park, established to protect the headwaters of the S?o francisco River and other places of scenic and ecological interest, is extensively visited due to its many tourist attractions, such as waterfalls, fauna and flora. An analysis of the park’s geography is needed to assess the risk involved in and its suitability for public use due to its territorial extension and environmental complexities. Thus, this study aimed to investigate the background of issues of interest to CU management. Additionally, we used high-resolution Rapideye imagery, altimetry and database of park infrastructure to build geospatial database and estimate classes of suitability for and risk in public use through GIS tools. The resulting cartographic data can support the planning of policies concerning the landscape and park’s territorial management.

关键词： Conservation Units Environment GIS Rapideye Imagery

Production of Lettuce Seedlings with Association of Diazotrophic Bacteria and Humic Acids

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American Journal of Plant Sciences 2018年第5期9卷 946-957页

作者： Carlos Augusto Rocha de Moraes Rego Bruna Penha Costa Marinez Carpiski Sampaio Marcos Massuo Kashiwaqui joao Henrique Silva Caetano Vandeir francisco Guimaraes Juan López de Herrera Pablo Wenderson Ribeiro Coutinho Cláudio Yuji Tsutsumi Maria Soraia Fortado Vera Cruz Thatiane Nepomuceno Alves Shirlene Souza oliveira Ana Carolina Pinguelli Ristau Hannah Braz Center of Agrarian Sciences State University of Western ParanáMarechal Candido RondonBrazil University School of Agricultural Technical Engineering Polytechnic University of MadridMadridSpain

The objective of this work was to verify the responses in agronomic and physiological characters of lettuce seedlings when submitted to different doses of humic acids and the presence or absence of inoculation of the bacterium Azospirillum brasilense. The experiment was carried out at the Protected Cultivation Station and Biological Control Teacher Mário César Lopes, belonging to the Experimental Stations Nucleus of the State University of the West of Paraná. The experimental design was a randomized block design, in a 5 × 2 factorial scheme, with four repetitions, totaling 40 plots. The first factor consisted of five solutions containing humic acid (100%, 75%, 50%, 25% and 0%) and the second factor was the presence or absence of the diazotrophic bacterium Azospirillum brasilense obtained by commercial product. Germination tests were carried out in addition to the determination of agronomic characters such as the number of fully expanded leaves, plant height, stem diameter, main root length, shoot dry mass, root dry mass, total dry mass, and Dickson quality index. The association of diazotrophic bacteria and humic acids did not influence the emergency rate, mean emergence time, mean speed of emergency, emergence speed index, plant height, and root length of lettuce seedlings. The dilutions of humic acids, regardless of inoculation with bacteria diazotrophic favored the development of the number of leaves, stem diameter and Dickson quality index. Inoculation of lettuce seeds with diazotrophic bacteria associated with the addition of humic acids, favored the development of the dry mass of root and total dry matter.

关键词： Azospirillum brasilense Humic Substances Lactuca sativa L. Productive Characteristics

Neutrophile-to-lymphocyte,lymphocyte-to-monocyte,and platelet-tolymphocyte ratios as prognostic and response biomarkers for resectable locally advanced gastric cancer

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World Journal of Gastrointestinal Oncology 2022年第7期14卷 1307-1323页

作者： Tiago Cruz Tomás Ines Eiriz Marina Vitorino Rodrigo Vicente joao Gramaca Alicia Guadalupe oliveira Paulo Luz Mafalda Baleiras Ana Sofia Spencer Luísa Leal Costa Patrícia Liu Joana Mendonca Magno Dinis Teresa Padrao Marisol Correia Goncalo Atalaia Michelle Silva Teresa Fiúza department of Medical Oncology Hospital Professor Doutor Fernando Fonseca EPEAmadora 2720-276Portugal department of Medical Oncology Centro Hospitalar Barreiro-Montijo EPEBarreiro 2830-003Portugal department of Medical Oncology Hospital do Espírito Santo deÉvora EPEÉvora 7000-811Portugal department of Medical Oncology Centro Hospitalar Universitário do Algarve EPEAlgarve 8000-386Portugal department of Medical Oncology Hospital São Francisco XavierCentro Hospitalar Lisboa Ocidental EPELisboa 1449-005Portugal department of Medical Oncology Hospital Santo António dos CapuchosCentro Hospital Lisboa Central EPELisboa 1169-050Portugal department of Medical Oncology Hospital BeatrizÂngeloLoures 2674-514Portugal department of Medical Oncology Centro Hospitalar de Trás-os-Montes e Alto Douro EPEVila Real 5000-508Portugal department of Medical Oncology Hospital da Senhora da Oliveira EPEGuimarães 4835-044Portugal department of Medical Oncology Hospital Garcia de Orta EPEAlmada 2805-267Portugal department of Medical Oncology Hospital da LuzLisboa 1500-650Portugal department of Medical Oncology Hospital Distrital de Santarém EPESantarém 2005-177Portugal

BACKGROUND Perioperative fluorouracil plus leucovorin,oxaliplatin,and docetaxel(FLOT)improves prognosis in locally advanced gastric cancer(LAGC).Neutrophil-to-lymphocyte(NLR),lymphocyte-tomonocyte(LMR),and platelet-to-lymphocyte(PLR)ratios are prognostic biomarkers but not predictive *** To assess blood ratios’(NLR,LMR and PLR)potential predictive response to FLOT and survival outcomes in resectable LAGC *** This was a multicentric retrospective study investigating the clinical potential of NLR,LMR,and PLR in resectable LAGC patients,treated with at least one preoperative FLOT cycle,from 12 Portuguese *** were compared through non-parametric Mann-Whitney *** operating characteristic curve analysis defined the cut-off values as:High PLR>141 for progression and>144 for mortality;high LMR>3.56 for T stage regression(TSR).Poisson and Cox regression models the calculated relative risks/hazard ratios,using NLR,pathologic complete response,TSR,and tumor regression grade(TRG)as independent variables,and overall survival(OS)as the dependent *** This study included 295 patients(mean age,63.7 years;59.7% males).NLR was correlated with survival time(r=0.143,P=0.014).PLR was associated with systemic progression during FLOT(P=0.022)and mortality(P=0.013),with high PLR patients having a 2.2-times higher risk of progression[95% confidence interval(CI):0.89-5.26]and 1.5-times higher risk of mortality(95%CI:0.92-2.55).LMR was associated with TSR,and high LMR patients had a 1.4-times higher risk of achieving TSR(95%CI:1.01-1.99).OS benefit was found with TSR(P=0.015)and partial/complete TRG(Pdence of pathological response had 2.1-times(95%CI:1.14-3.96)and 2.8-times(95%CI:1.6-5)higher risk of *** Higher NLR is correlated with longer survival *** LMR patients have a higher risk of decreasing T stage,whereas high PLR patients have higher odds of progressing under FLOT a

关键词： Gastric cancer Perioperative fluorouracil plus leucovorin,oxaliplatin,and docetaxel Neutrophil-to-lymphocyte Lymphocyte-to-monocyte Platelet-to-lymphocyte Tumor regression grade

Study of Wastewater Treatment Plants in Operation with UASB Reactors in the Municipality of Juazeiro do Norte-Ceará

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Journal of Environmental Science and Engineering(B) 2018年第6期7卷 215-223页

作者： francisco de Assis Martins Ponce joao Paulo Leite Félix AndréGadelha de oliveira Ricardo Leandro Santos Araújo Center of Technological Sciences University of FortalezaFortaleza 60811-905Brazil department of Civil Construction Federal Institute of EducationScience and Technology of CearáFortaleza 60040-531Brazil

The indiscriminate release of effluents without any type of treatment,or previous treatment without adequate disinfection,can cause several inconveniences for the water body and society,including water-borne *** the present study,the physicochemical and biological aspects of three WWTPs(Wastewater Treatment Plants)operated with UASB(Upflow Anaerobic Sludge Blanket)technology in Juazeiro do Norte were analyzed in order to verify the state of CearáState legislation,compliance with standards for the release of domestic effluents into bodies’*** the research,it verified that the stations did not meet the effluent release standards for TSS(Total Suspended Solids)and removal of Escherichia coli in several analyzed *** also verified that the UASB stations need a post-treatment in order to assist in the process of removal of organic matter and pathogens.

关键词： Effluent UASB analyze