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Assessment of Genetic Diversity of Drought Tolerant and Susceptible Rice Genotypes Using Microsatellite Markers

Rice science 2019年第4期26卷 239-247,I0005,I0010-I0014页

作者： Ravindra DONDE jitendra kumar Gayatri GOUDA Manoj kumar GUPTA Mitadru MUKHERJEE Sk Yasin BAKSH Pradosh MAHADANI Khirod kumar SAHOO Lambodar BEHERA Sushanta kumar DASH Crop Improvement Division Indian Council of Agricultural Research-National Rice Research Institute/Formerly-Central Rice Research Institute Cuttack Pin-753006.OdishaIndia Ravenshaw University Department of Biotechnology Cuttack Pin-753003Odisha India Department of Biotechnology & Bioinformatics Yogi Vemana University KadapaPin-516003 OdishaIndia

The introgression of wild chromosomal segments into popular rice varieties is one of the potential approaches for developing varieties for drought stress condition. Sixteen genotypes, including nine indica, two tropical japonica and five chromosome segments substitution lines (CSSLs) with different levels of tolerance/susceptibility to drought stress, were selected for diversity study. Sixty-three microsatellite markers were utilized for assessing genetic diversity. A total of 95 alleles were amplified, and out of them, 60 were polymorphic. Six unique alleles, amplified by the microsatellite loci RM276, RM472, RM488, RM537, RM541 and RM28089, were identified in six genotypes, namely FR13A, Brahamanakhi, RUF44, Swarna-sub1, Brahamanakhi and Satyabhama. The highest genetic similarity was found among CSSLs. Polymorphism information content (PIC) value varied from 0 to 1.00 with an average of 0.66 per locus. Twenty-eight microsatellites were found to be polymorphic, which could be used in marker-assisted selection programme. All the sixteen genotypes were grouped into two major clusters at genetic similarity of 0.64. In the cluster I, five CSSLs identified as diverse genotypes had wild ancestor segments responsible for drought tolerance, and hence they could be utilized as potential donors. The popular Indian varieties, Swarna-sub1 and IR64-sub1, could be used as recurrent parents in the future breeding program for developing varieties for abiotic stresses such as submergence and drought.

关键词： genetic diversity marker-assisted selection microsatellite marker rice drought stress submergence chromosome segment substitution line

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Organizational dynamics of an information system: case study from the forestry sector

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Journal of Forestry Research 2007年第1期18卷 1-10页

作者： Rajiv kumar Garg jitendra kumar Das Mohit Gera Uttar Pradesh Forest Department Lucknow-226010INDIA Indian Institute of Management Lucknow - 226016INDIA Indira Gandhi National Forest Academy Dehradun - 248006INDIA

The Forest Department in the State of Uttar Pradesh, India developed Forest Management Information System (FMIS) for achieving organizational goals of improved financial and human resource management, improvement in the management of forests and wildlife, and for achieving responsive administration. This paper, based on field research, presents an assessment of the dynamics of FMIS in organizational context for a better understanding of such systems in forestry organizations. The paper also investigates the success of FMIS in assisting decision makers in achieving organizational goals. Based on the knowledge developed during the course of the study, key learning elements have been highlighted for the benefit of the stakeholders in information systems in forest sector.

关键词： Business process reengineering Culture change Human resource Information system Socio-economic profile Strategic management

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QTL analysis for some quantitative traits in bread wheat

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第11期8卷 807-814页

作者： PUSHPENDRA kumar Gupta HARINDRA Singh Balyan PAWAN Laxminarayan Kulwal NEERAJ kumar AJAY kumar REYAZUL Rouf Mir AMITA Mohan jitendra kumar Molecular Biology Laboratory Department of Genetics and Plant BreedingChaudhary Charan Singh UniversityMeerut 250 004India

Quantitative trait loci (QTL) analysis was conducted in bread wheat for 14 important traits utilizing data from four different mapping populations involving different approaches of QTL analysis. Analysis for grain protein content (GPC) sug- gested that the major part of genetic variation for this trait is due to environmental interactions. In contrast, pre-harvest sprouting tolerance (PHST) was controlled mainly by main effect QTL (M-QTL) with very little genetic variation due to environmental interactions; a major QTL for PHST was detected on chromosome arm 3AL. For grain weight, one QTL each was detected on chromosome arms 1AS, 2BS and 7AS. QTL for 4 growth related traits taken together detected by different methods ranged from 37 to 40; nine QTL that were detected by single-locus as well as two-locus analyses were all M-QTL. Similarly, single-locus and two-locus QTL analyses for seven yield and yield contributing traits in two populations respectively allowed detection of 25 and 50 QTL by composite interval mapping (CIM), 16 and 25 QTL by multiple-trait composite interval mapping (MCIM) and 38 and 37 QTL by two-locus analyses. These studies should prove useful in QTL cloning and wheat improvement through marker aided selection.

关键词： Quantitative trait loci (QTL) analysis Grain quality traits Grain protein content Pre-harvest sprouting tolerance(PHST) Grain weight Mapping populations Bread wheat

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Recent advances in chronic granulomatous disease

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Genes & Diseases 2020年第1期7卷 84-92页

作者： Gummadi Anjani Pandiarajan Vignesh Vibhu Joshi jitendra kumar Shandilya Dharmagat Bhattarai Jyoti Sharma Amit Rawat Allergy Immunology Unit Department of PediatricsAdvanced Pediatrics CentrePostgraduate Institute of Medical Education and ResearchChandigarhIndia

Chronic granulomatous disease(CGD)is an inherited defect of phagocyte function due to defective NADPH *** with CGD are not able to effectively clear the infections because of the defect in the phagocyte production of oxygen free radicals and are prone to recurrent bacterial and fungal *** complications are also noted in CGD such as colitis,non-infective granulomas causing gastrointestinal or urinary tract obstruction,hemophagocytic lymphohistiocytosis,and *** on toll-like receptor pathways and neutrophil extracellular traps in CGD have shed light on the role of NADPH oxidase in the innate immunity and pathogenesis of infections in *** reports also indicate a reduction of memory B cells and defective production of functional antibodies in *** the exact mechanisms for non-infective inflammatory complications in CGD are not yet clear,studies on efferocytosis and defective autophagy with inflammasome activation have made a substantial contribution to our understanding of the pathogenesis of inflammation in *** also discuss the clinical and molecular features of p40phox defects and a newer genetic defect,*** phenotypes of X-linked carriers of CYBB are also discussed.

关键词： Chronic granulomatous disease Colitis EROS Genetics Infections Inflammation p40phox

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Draft genome sequence of a less-known wild Vigna: Beach pea(V. marina cv. ANBp-14-03)

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The Crop Journal 2019年第5期7卷 660-666页

作者： Awnindra kumar Singh A.Velmurugan Debjyoti Sen Gupta jitendra kumar Ravi Kesari Aravind Konda Narendra Pratap Singh Sibnarayan Dam Roy Utpal Biswas R.Rahul kumar Sanjay Singh ICAR-Central Island Agricultural Research Institute Port Blair 744101Andaman&Nicobar IslandsIndia Division of Crop Improvement ICAR-Indian Institute of Pulses ResearchKanpur 208024Uttar PradeshIndia Department of Plant Breeding and Genetics Bhola Paswan Shastri Agricultural CollegePurneaBihar 854302India Division of Plant Biotechnology ICAR-Indian Institute of Pulses ResearchKanpur 208024Uttar PradeshIndia ICAR-National Research Centre for Biotechnology LBS CentrePusa CampusNew Delhi 110012India

Beach pea or beach cowpea(Vigna marina(Burm.)Merr.)belongs to the family *** is a close relative of cultivated Vigna species such as adzuki bean(***),cowpea(***),mung bean(***),and blackgram(***),and is distributed throughout the *** its ability to tolerate salt stress,beach pea has great potential to contribute salt-tolerance genes for developing salt-tolerant cultivars in cultivated Vigna ***,it is still underutilized in Vigna breeding programs.A draft genome sequence of beach pea was generated using a high-throughput next-generation sequencing platform,yielding 23.7 Gb of sequence from 79,929,868 filtered reads.A de novo genome assembly containing 68,731 scaffolds gave an N50 length of 10,272 bp and the assembled sequences totaled 365.6 Mb.A total of 35,448 SSRs,including 3574 compound SSRs,were identified and primer pairs for most of these SSRs were *** analysis identified 50,670 genes with mean coding sequence length 1042 *** analysis revealed highest sequence similarity with ***,followed by *** with the *** genome revealed 16,699 SNPs and 2253 InDels and comparison with the *** genome revealed 17,538 SNPs and 2300 *** our knowledge this is the first draft genome sequence of beach pea derived from an accession(ANBp-14-03)adapted locally in the Andaman and Nicobar Islands of *** draft genome sequence may facilitate the genetic enhancement in cultivated Vigna species.

关键词： Beach pea Vigna marina NextSeq 500 Whole-genome sequencing Salinity tolerance

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Urban Growth and Its Impact on Cityscape: A Geospatial Analysis of Rohtak City, India

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Journal of Geographic Information System 2012年第1期4卷 12-19页

作者： Nina Singh jitendra kumar 不详

Rohtak is one of the eight priority towns (Regional Centres) of National Capital Region (NCR) Regional Plan 2001. It lies 70 kms north-west from Delhi, the National Capital of India. Located at 28?41'1'' North latitude and 76?12'42'' East longitude in the NCR region of Haryana state on National Highway No. 10, it spreads over 100.57 km2. Its population was projected to grow to 500,000 by 2001. Despite its nearness to the national capital it did not attract investment and the rate of urbanization remained low. Rohtak city grew slower than the state of Haryana in the three decades ending in 2001. On being declared as Municipal Corporation in 2010 urban growth of Rohtak took place in the form of extension in territorial jurisdiction and inclusion of urbanized eight villages. The new economic environment demands sustainable land management. Spatial information of land use/land cover types and their change detection in time series are important means for city planning and undertaking development activities. The present work is undertaken in that spirit. It has analyzed the relationship between urban growth and land use changes and their impact on Rohtak city. The period of focus is from 1983 to 2010. Both primary and secondary sources of data have been used for the present study. Secondary sources of data—guide map, topographical sheet and high resolution satellite imageries have been used to detect land use/land cover changes from the study area whereas primary source of data include ground truth and photographs from the field.

关键词： Geospatial Technology Cityscape NUIS NCR HUDA

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Nutech functional score: A novel scoring system to assess spinal cord injury patients

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World Journal of Methodology 2017年第2期7卷 68-72页

作者： Geeta Shroff jitendra kumar Barthakur Nutech Mediworld Department of Statistics Nutech Mediworld

AIM To develop a new scoring system, nutech functional scores(NFS) for assessing the patients with spinal cord injury(SCI).METHODS The conventional scale, American Spinal Injury Association's(ASIA) impairment scale is a measure which precisely describes the severity of the ***, it has various limitations which lead to incomplete assessment of SCI *** have developed a 63 point scoring system, i.e., NFS for patients suffering with SCI.A list of symptoms either common or rare that were found to be associated with SCI was recorded for each *** the basis of these lists, we have developed *** These lists served as a base to prepare NFS, a 63 point positional(each symptom is sub-graded and get points based on position) and directional(moves in direction BAD → GOOD) scoring *** non-progressive diseases, 1, 2, 3, 4, 5 denote worst, bad, moderate, good and best(normal), *** for SCI has been divided into different groups based on the affected part of the body being assessed, i.e., motor assessment(shoulders, elbow, wrist, fingers-grasp, fingers-release, hip, knee, ankle and toe), sensory assessment, autonomic assessment, bed sore assessment and general *** probability based studies required a range of(-1, 1) or at least the range of(0, 1) to be useful for real world analysis, the grades were converted to respective numeric *** NFS can be considered as a unique tool to assess the improvement in patients with SCI as it overcomes the limitations of ASIA impairment scale.

关键词： Spinal cord injury American Spinal Injury Association’s Impairment Scale Nutech functional score Comparison of assessment Positional scoring system

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Impact of Selenium on Microtubules Polymarisation of Spermatozoa of Endosulfan Exposed Swiss Albino Mice

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Journal of Life Sciences 2011年第2期5卷 132-135页

作者： Ranjit kumar jitendra kumar Singh Mohammad Ali Arun kumar Mahavir Cancer Sansthan ＆ Research Centre Phulwarisharif Patna 801505 India

Pesticide plays a vital role in agricultural, industrial and residemial pest control. In recent years the ‘pesticide problem＇ has been the focus of public interest because these chemicals have a very important role in agriculture but these chemicals also act like endocrine disruptor. Experiments were carried out on 12 weeks old mice with 30 ± 2 gm. b.w. Endosulfan were administered at 3 mg/kg b.w daily by gavage method for five weeks followed by selenium treatment at 10 μg/kg.b.w./day for five weeks. 9 ＋ 2 arrangment were clearly observed in control group mice with well structured dynein and nexin arm while Endosulfan treated group show depolymerised microtubule and 9 ＋ 1 arrangment with degenerated mitochondria. While selenium treated group shows restoration of 9 ＋ 2 arrangement of microtubule. It is evident from study that endosulfan causes depolymerisation of microtubule while selenium administered group show normal polymerized microtubule and restores normal fertility in mice.

关键词： Spermatozoa selenium microtubules dynein arm nexin axoneme depolymerisation Swiss albino mice

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Ultrasonic Attenuation in Calcium Oxide

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Advances in Materials Physics and Chemistry 2011年第2期1卷 44-49页

作者： jitendra kumar Kailash R. Sanjeev kumar Shrivastava Devraj Singh Virendra kumar 不详

Ultrasonic attenuation studies can be used to characterize material not only after production but during processing as well. The most important causes of ultrasonic attenuation in solids are electron-phonon, phonon-phonon interaction and that due to thermo elastic relaxation. The two dominant processes that will give rise to appreciable ultrasonic attenuation at higher temperature are the phonon-phonon interaction also known as Akhiezer loss and that due to thermo elastic relaxation are observed in calcium oxide crystal. At frequencies of ultrasonic range and at higher temperatures in solids, phonon-phonon interaction mechanism is dominating cause for attenuation. Ultrasonic attenuation due to phonon-phonon interaction (α/f2)p-p and thermo elastic relaxation (α/f2)th are evaluated in Calcium Oxide crystal up to an elevated temperature from 100 K - 1500 K along , and crystallographic directions. Temperature dependence of ultrasonic attenuation along different crystallographic direction reveals some typical characteristic features.

关键词： Attenuation Thermo Elastic Relaxation Phonon-Phonon Interaction Akheiser Loss Gruneisen Constants

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Endosulfan Causes Neoplastic Changes in the Liver Cells of Mice

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Open Journal of Pathology 2014年第3期4卷 86-93页

作者： Arun kumar Mohammad Ali jitendra kumar Singh Akhileshwari Nath Sudhir kumar Srivastava Ranjit kumar Department of Zoology Patna University Patna India Research Centre Mahavir Institute and Research Centre Patna India

The rapid growth in global population continues to challenge the world’s ability to provide enough food. As one of the most crucial issues for human development, food production must increase to offset hunger and poverty as well as social unrest. To augment the yield of crops a variety of pesticides like Endosulfan, Rogor, Aldrin, Chlorpyrifos, etc. are being used liberally by the farmers. In the present investigation, Endosulfan was administered orally (daily) by gavage method to female Swiss albino mice group for 4 weeks @ 3.0 mg/kg b.w. After that, they were left for 6 months and then sacrificed and liver tissues were fixed for light microscopy and Transmission Electron Microscopic study. The histopathological study of Endosulfan administered group liver showed hepatocytes with congestion in central vein with less dense cytoplasm, haemorrhaged bile duct, degenerated cytoplasm and central vein with vacuolations in sinusoidal spaces. Neoplastic changes in hepatocytes are the major finding of study. The ultrastuctural study revealed dilation in the nuclear pore complex and massive movement of cytoplasmic material from cytoplasm to the nucleus which is major finding which denotes neoplastic changes. Presence of abundant free lying polyribosomes in the cytoplasm, which denotes neoplastic changes in the cellis also one of theimportant finding observed. The present study thus deciphers that Endosulfan toxicity leads to onset of neoplasia thence carcinogenesis in liver cells in Swiss albino mice which is the novel finding in the field of toxicology.

关键词： Endosulfan Neoplasia Liver Cell Light Microscopy Transmission Electron Microscopy

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