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Atomic force microscopic observation on substructure of pollen exine in Cedrus deodara and Metasequoia glyptostroboides

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Chinese Science Bulletin 2000年第16期45卷 1500-1503页

作者： XING Shuping LI Bingshi WANG Chen HU Yuxi lin jinxing Institute of Botany Chinese Academy of SciencesBeijing 100093China Institute of Chemistry Chinese Academy of SciencesBeijing 100080China

The substructure of pollen exine in Cedrus deodara (Roxb.) Loud, and Metasequoia glyptostroboides Hu et Cheng has been examined with an atomic force microscope (AFM). The results indicate that the exine substructure units containing sporopollenin in two species are similar in shape, which are granular, but slightly different in size. In Cedrus the substructure unit of pollen exine appears to be 56-99 nm long and 42-74 nm wide, while in Metasequoia it appears to be 81-118 nm long and 43-98 nm wide. It has been observed that the subunits of pollen exine in Cedrus arranged tightly to form short-rod-like or spheroidal pollen exine units, several or more than ten of which formed an island-like structure. There are various spaces among these island-like structures which are interconnected to occupy the entire pollen exine. In Metasequoia, the subunits of pollen exine also arranged tightly with a distribution tendency of cluster of 3-10, however, no obvious boundary exists among these clusters. From our

关键词： Cedrus deodara Metasequoia glyptostroboides atomic force microscope pollen exine substructure sporopollenin.

An Effective and Inducible System of TAL Effector-Mediated Transcriptional Repression in Arabidopsis

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Molecular Plant 2016年第11期9卷 1546-1549页

作者： Sen lin Yuanyuan Zhao Yingfang Zhu Michael Gosney Xin Deng Xiaohua Wang jinxing lin Key Laboratory of Plant Resources Institute of Botany Chinese Academy of Sciences Beijing 100093 China University of Chinese Academy of Sciences Beijing 100049 China College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 China Department of Horticulture and Landscape Architecture Department of Botany and Plant PathologyPurdue University West Lafayette IN 47907 USA These authors contributed equally to this article

Dear Editor,Gene regulation in model organisms is critical for gene function analysis and is thus essential for human health and agricultural production. Therefore, several molecular tools have been developed to regulate gene expression at the transcriptional and post-transcriptional levels. In recent years, the transcription activator-like effector （TALE）, which is predominantly found in Xanthomonas.

关键词：黄单胞菌属基因基因表达拟南芥属社会控制正式胰岛素转录遗传人类

Relationship between stomatal density and the changes of atmospheric CO_2 concentrations

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Chinese Science Bulletin 1998年第11期43卷 928-930页

作者： HE Xinqiang, lin Yuehui, lin jinxing and HU YuxiInstitute of Botany, Chinese Academy of Sciences, Beijing, 100093, China Institute of Botany Chinese Academy of Sciences Beijing China

The relationship between the stomatal density of five woody plants endemic to China, i.e. Eucommia ulmoides, Quercus liaotungensis, Q. glandulifera var. brevipetiolata, Cyclocarya paliurus and Ficus heteromorpha, and the atmospheric CO-2 concentrations was studied by observations on leaves of the herbarium-stored specimens(1920s-1990s). The results showed that the stomatal density in Eucommia ulmoides, Quercus liaotungensis and Q. glandulifera% var. brevipetiolata decreased significantly in response to the elevated atmospheric CO-2 concentrations, while in Cyclocarya paliurus it decreased slightly and in Ficus heteromorpha there were no responses.

关键词： woody plants stomatal density atmospheric CO-2 concentrations.

Effects of stem structure andcell wall components on bending strength in wheat

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Chinese Science Bulletin 2006年第7期51卷 815-823页

作者： WANG Jian ZHU Jinmao lin Qinqin LI Xiaojuan TENG Nianjun LI Zhensheng LI Bin ZHANG Aimin lin jinxing Key Laboratory of Photosynthesis and Molecular Environment Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China College of Life Sciences Fujian Normal University Fuzhou 350007 China The State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China

Morphological traits, anatomical features, chemical components and bending stress in the stems of three genotypes of wheat (Triticum aestivum L.), namely Xiaoyan54, 8602 and Xiaoyan81, were ex- amined by means of light microscopy coupled with Fourier transform infrared spectroscopy (FTIR). No- ticeable changes in morphological and anatomical traits were observed, including outer radius of stem, the ratio of stem outer radius to stem wall thickness, various tissue proportions and variations among dif- ferent types of vascular bundles. The results of chemical analysis revealed that Xiaoyan81 had the highest cellulose content in comparison with Xiao- yan54 and 8602, whereas lignin level in Xiaoyan81 was lower than that in 8602 but higher that that in Xiaoyan54. Bending stress analysis demonstrated that Xiaoyan81 may be the main target for identifica- tion, for it had the highest bending stress among the stems of three genotypes. Associated with bending stress, all the results presented here suggested that the ratio of stem wall thickness to its outer radius, schlerenchyma tissue proportion, the average num- ber of big VB per unit and the cellulose content are four important factors affecting the mechanical strength of Xiaoyan81 wheat stems, which can be considered as the key parameters for selecting varie- ties with bending stress. Therefore, it was suggested that in the selection of lodging resistant cultivars one should consider those characterized with large ratio of outer radius of stem to stem wall thickness, great-erschlerenchyma tissue proportion, high average number of big VB per unit with high cellulose content in their stems.

关键词：弯曲应力小麦茎维管束纤维素木质素

Tracking Tonoplast Protein Behaviors in Intact Vacuoles Isolated from Arabidopsis Leaves

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Molecular Plant 2017年第2期10卷 349-352页

作者： Lv, Xueqin Jing, Yanping Wu, Hongyang lin, jinxing Beijing Forestry Univ Coll Biol Sci & Biotechnol Beijing 100083 Peoples R China

Dear Editor Membrane proteins perform a vast array of functions in vital cellular activities, including transport of molecules, responses to stimuli, and regulation of immune reactions （Proft et al., 2006; Bohm et al., 2014）. Real-time behaviors of proteins, such as their lateral diffusion and dynamic distribution, affect their ac- tivity and functions （Bai et al., 2008）. Consequently, the tracking and analysis of the behaviors of membrane proteins in living cells can further our understanding of the mechanisms regulating the biological functions of proteins.

关键词： Tonoplast Vacuoles Leaves

Three-dimensional reconstruction of Picea wilsonii Mast. pollen grains using automated electron microscopy

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Science China(Life Sciences) 2020年第2期63卷 171-179页

作者： Weiwei Shen lingyu Ma Xi Zhang Xixia Li Yuanyuan Zhao Yanping Jing Yun Feng Xueke Tan Fei Sun jinxing lin Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing Forestry UniversityBeijing 100083China College of Biological Sciences&Biotechnology Beijing Forestry UniversityBeijing 100083China Center for Biological Imaging Institute of BiophysicsChinese Academy of SciencesBeijing 100083China

Three-dimensional electron microscopy(3 D-EM) has attracted considerable attention because of its ability to provide detailed information with respect to developmental analysis. However, large-scale high-resolution 3 D reconstruction of biological samples remains challenging. Herein, we present a 3 D view of a Picea wilsonii Mast. pollen grain with 100 nm axial and38.57 nm lateral resolution using AutoCUTS-SEM(automatic collector of ultrathin sections-scanning electron microscopy). We established a library of 3,127 100 nm thick serial sections of pollen grains for preservation and observation, demonstrating that the protocol can be used to analyze large-volume samples. After obtaining the SEM images, we reconstructed an entire pollen grain comprising 734 serial sections. The images produced by 3D reconstruction clearly revealed the main components of the *** pollen grain, i.e., two sacci and pollen corpus, tube cell, generative cell, and two degenerated prothallial cells, and their internal organization. In addition, we performed a quantitative analysis of the different pollen grain cells, including sacci, and found that there were 202 connections within a saccus SEM image. Thus, for the first time, this study provided a global 3D view of the entire pollen grain, which will be useful for analyzing pollen development and growth.

关键词： 3D reconstruction AutoCUTS-SEM electron microscopy pollen

Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy

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Molecular Plant 2016年第9期9卷 1229-1239页

作者： Xiaojuan Li Jingjing Xing Zongbo Qiu Qihua He jinxing lin College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 China Key Laboratory of Plant Resources Institute of Botany Chinese Academy of Sciences Beijing 100093 China University of Chinese Academy of Sciences Beijing 10049 China College of Life Sciences Henan Normal University Xinxiang 453007 China The Health Science Center Peking University Beijing 100191 China These authors contributed equally to this article.

Deciphering the dynamics of protein and lipid molecules on appropriate spatial and temporal scales may shed light on protein function and membrane organization. However, traditional bulk approaches cannot unambiguously quantify the extremely diverse mobility and interactions of proteins in living cells. Fluores- cence correlation spectroscopy （FCS） is a powerful technique to describe events that occur at the singlemolecule level and on the nanosecond to second timescales; therefore, FCS can provide data on the heterogeneous organization of membrane systems. FCS can also be combined with other microscopy techniques, such as super-resolution techniques. More importantly, FCS is minimally invasive, which makes it an ideal approach to detect the heterogeneous distribution and dynamics of key proteins during development. In this review, we give a brief introduction about the development of FCS and summarize the significant contributions of FCS in understanding the organization of plant cell membranes and the dy- namics and interactions of membrane proteins .We also discuss the potential applications of this technique in plant biology.

关键词： fluorescence correlation spectroscopy microdomain membrane protein dynamics interaction

Networked Control System Time Delay Compensation Based on Improved Implicit GPC

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Networked Control System Time Delay Compensation Based on Im...

Subcellular Redistribution of Root Aquaporins nduced by Hydrogen Peroxide

第36届中国控制会议

作者： Sun Chao Zhou Ying Dai Junxiang lin jinxing Nanjing University of Posts and Telecommunications College of Automation

ISBN: (纸本)9781538629185

The ELM is used to predict the delay,and combines with implicit generalized predictive control（IGPC） to compensate time delay in this *** random time-delay in the networked control system（NCS） can usually deteriorate the control performance and stability of networked control *** order to solve this problem,this paper puts forward the networked time delay in a short time based on extreme learning machine（ELM）.At the same time,IGPC is used in this networked control system with the results of *** simulation results show that the method in this paper make the output of the system track the reference trajectory well and performance better than traditional generalized prediction algorithm.

关键词： networked control system implicit generalized prediction algorithm extreme learning machine time delay compensation

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Molecular Plant 2015年第7期8卷 1103-1114页

作者： Michael M. Wudick Xiaojuan Li Valeria Valentini Niko Geldner Joanne Chory jinxing lin Christophe Maurel Doan-Trung Luu Biochimie et Physiologie Moleculaire des Plantes Institut de Biologie Integrative des Plantes UMR 5004 CNRS/UMR 0386 INRA/Montpellier SupAgro/Universit~Montpellier 2 2 Place Viala F-34060 Montpellier Cedex 2 France Key Laboratory of Plant Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China Department of Plant Molecular Biology Universite de Lausanne 1015 Lausanne Switzerland Plant Molecular and Cellular Biology Laboratory The Salk Institute La Jolla CA 92037 USA Present address: Department of Cell Biology and Molecular Genetics University of Maryland College Park MD 20742-5815 USA Present address: Instituto Gulbenkian de Ciencia 2780-156 Oeiras Portugal

Aquaporins are water channel proteins that mediate the fine-tuning of cell membrane water permeability during development or in response to environmental stresses. The present work focuses on the oxidative stress-induced redistribution of plasma membrane intrinsic protein （PIP） aquaporins from the plasma membrane （PM） to intracellular membranes. This process was investigated in the Arabidopsis root. Su- crose density gradient centrifugation showed that exposure of roots to 0.5 mM H2O2 induces significant depletion in PM fractions of several abundant PIP homologs after 15 rain. Analyses by single-particle tracking and fluorescence correlative spectroscopy showed that, in the PM of epidermal cells, H2O2 treat- ment induces an increase in lateral motion and a reduction in the density of a fluorescently tagged form of the prototypal AtPIP2;1 isoform, respectively. Co-expression analyses of AtPIP2;1 with endomembrane markers revealed that H2O2 triggers AtPIP2;1 accumulation in the late endosomal compartments. Life- time analyses established that the high stability of PIPs was maintained under oxidative stress conditions, suggesting that H2O2 triggers a mechanism for intracellular sequestration of PM aquaporins without further degradation. In addition to information on cellular regulation of aquaporins, this study provides novel and complementary insights into the dynamic remodeling of plant internal membranes during oxida- tive stress responses.

关键词： Arabidopsis aquaporin stress reactive oxygen species root