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Proteomic mechanism of Bacillus cereus endospore against plasma-activated water(PAW)

Food Science and Human Wellness 2024年

作者： Xiao Hu jinsong feng Xinyu Liao Tian Ding Ruiling Lü School of Biosystems Engineering and Food Science Zhejiang University Future Food Laboratory Innovation Center of Yangtze River Delta Zhejiang University Ningbo Innovation Center Zhejiang University

Plasma-activated water (PAW) indicated promising potential in controlling the biological contamination of Bacillus cereus， which eliminated its evolutionary endospore that improves its survival ability. However， the spore inactivation mechanism by PAW at molecular level was not well understood. The mechanism of the Bacillus cereus endospore against PAW at proteomic levels was demonstrated. The Tandem Mass Tag (TMT) labeling was performed. By comparing the treatment groups with control (including PAW and PAW added superoxide dismutase (SOD)) ， the expression of 251 proteins (with the number of 207 up- and 44 down-regulated) and 379 proteins (with the corresponding number of 238 and 141) were drastically affected， separately. The six categories based on the protein-protein interaction (PPI) networks included oxidation-reduction， transport， sporulation and DNA topological change， gene expression， metabolism， and others. The three dehydrogenases (Gene hisD， BC＿2176， and asd) in PAW while oxidoreductase (Gene BC＿0399 and BC＿2529) in SOD were activated to maintain the antioxidation of spores. The proteins (BC＿4271 and BC＿2655) in SOD were dramatically activated， which were involved in the carbohydrate， amino acid， and energy-coupling transport. All the small， acid-soluble spore proteins were activated in both groups to protect the spores’ DNA. In SOD， genes metG2 and rpmC also were considered important factors in translation while this role was played in gene groES but not rpmF in PAW. The PAW activated the biogenesis of cell wall/membrane/envelope and phosphorelay signal transduction system to contribute to the survival of spores whereas the SOD damaged these two processes as well as cell division， chromosome separation， organic acid phosphorylation， base- and nucleotide-excision repair to lead to the death of spores. This would promise to lay the foundation for advancing the study of the intrinsic mechanism of spore killing against PAW and can also provide a reference f

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Guest Editorial:Special Issue on Microbial Food Safety

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Food Quality and Safety 2022年第3期6卷 353-354页

作者： jinsong feng Tian Ding College of Biosystems Engineering and Food Science Zhejiang UniversityHangzhouChina

The contamination of food by pathogenic bacteria is a critical safety concern of public health *** recognized by various disease control and protection agencies,pathogenic strains of Escherichia coli,Listeria monocytogenes,Salmonella spp.,and Vibrio *** among the most common microbes that cause foodborne *** the past decades,a tremendous increase in foodborne disease outbreaks caused by pathogenic bacteria in food has been documented.

关键词： spp pathogenic food

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景观基因视角下历史文化街区保护与发展研究——以宜昌市织布街为例

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华中建筑 2024年第8期42卷 137-142页

作者：童文娟邓巍冯劲松华中科技大学建筑与城市规划学院湖北省城镇化工程技术研究中心湖北省规划设计研究总院有限责任公司

历史文化街区是城市历史发展的内在缩影与潜在见证,保护好历史文化街区的历史底蕴与特色风貌是目前城乡历史文化保护与传承工作的重要内容之一。该文以宜昌市织布街历史文化街区为例,基于景观基因理论,建立织布街景观基因识别指标体系,... 详细信息

历史文化街区是城市历史发展的内在缩影与潜在见证,保护好历史文化街区的历史底蕴与特色风貌是目前城乡历史文化保护与传承工作的重要内容之一。该文以宜昌市织布街历史文化街区为例,基于景观基因理论,建立织布街景观基因识别指标体系,从环境基因、布局基因、建筑基因以及文化基因四个方面提取织布街景观基因特征,并根据街区现状以及景观基因提取结果,从布局基因的传承与延续、建筑基因的保护与改造、以及文化基因的传承与发展三个方面探索织布街的保护与发展规划,以期为其他历史文化街区景观元素提取、风貌延续提供一定借鉴与参考。

关键词：景观基因历史文化街区织布街保护与发展

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食品工业中细菌生物被膜研究进展

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浙江大学学报（农业与生命科学版） 2024年第4期50卷 551-567页

作者：张润润张颀宜楼倚洋陈秀琴冯劲松丁甜浙江大学生物系统工程与食品科学学院浙江杭州310058 浙江大学长三角智慧绿洲创新中心浙江嘉兴314100

生物被膜是细菌在环境中的主要存在形式,是具有三维结构的微生物群落及极强胁迫耐受性的特殊生态。在食品加工及贮藏过程中食源性致病菌生物被膜易引起消毒逃逸及细菌污染,进而造成毒素积累或食品变质,威胁消费者健康安全。在食品工业中... 详细信息

生物被膜是细菌在环境中的主要存在形式,是具有三维结构的微生物群落及极强胁迫耐受性的特殊生态。在食品加工及贮藏过程中食源性致病菌生物被膜易引起消毒逃逸及细菌污染,进而造成毒素积累或食品变质,威胁消费者健康安全。在食品工业中,预防、抑制和清除食源性致病菌生物被膜是保证食品微生物安全的关键。因此,迫切需要找到高效阻控细菌生物被膜形成并有效清除生物被膜的方法和策略。本文综述了食品工业中生物被膜污染的现状及其胁迫耐受机制,并有针对性地讨论了食品加工领域中生物被膜阻控技术,有助于更清晰地认识由生物被膜造成的食品安全风险,为研发高效绿色的生物被膜清除技术提供了理论依据。

关键词：食源性致病菌生物被膜食品工业胁迫耐受性清除技术

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西部某气田井场分离器液相管线法兰腐蚀原因分析

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装备环境工程 2024年第2期21卷 112-118页

作者：陈庆国常泽亮吴超王帅丰劲松岳良武中国石油天然气股份有限公司塔里木油田分公司新疆库尔勒841000 中国石油集团工程材料研究院有限公司石油管材及装备材料服役行为与结构安全国家重点实验室西安710077 长江大学化学与环境工程学院湖北荆州434023

目的解决西部某气田井场分离器液相出口管线法兰严重腐蚀问题。方法通过宏观形貌观察、无损检测、化学成分分析、金相组织分析、力学性能测试、腐蚀区域微观形貌观察、腐蚀产物物相分析以及腐蚀电化学实验的方法,分析该法兰发生腐蚀... 详细信息

目的解决西部某气田井场分离器液相出口管线法兰严重腐蚀问题。方法通过宏观形貌观察、无损检测、化学成分分析、金相组织分析、力学性能测试、腐蚀区域微观形貌观察、腐蚀产物物相分析以及腐蚀电化学实验的方法,分析该法兰发生腐蚀的原因。结果 A105制法兰与316L制密封圈存在较强的电偶腐蚀倾向。结论电偶腐蚀是导致法兰面严重腐蚀的主要原因,另外,液相管线停用前放空不彻底,法兰底部存在积液,导致气液界面位置叠加发生水线腐蚀。根据法兰腐蚀原因提出了针对性的防腐建议。

关键词：气田分离器法兰电偶腐蚀电流密度电化学

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乳基零食中高风险微生物失活模型构建

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中国乳品工业 2024年第7期52卷 11-16页

作者：韩欣彤张润润张祁张红雨王旭王笃良赵琦裴晓燕李玲玲丁甜冯劲松浙江大学生物系统工程与食品科学学院杭州310058 浙江大学长三角智慧绿洲创新中心浙江嘉兴314102 国家乳业技术创新中心呼和浩特010110 内蒙古伊利实业集团股份有限公司呼和浩特010110 内蒙古乳业技术研究院有限责任公司呼和浩特010110

为了探究微波处理对含乳固态成型制品中高风险微生物的致死效果,将大肠杆菌、沙门氏菌、金黄色葡萄球菌、黑曲霉以及毛霉5种微生物分别接种到乳基零食中,进而采用微波设备处理样品,选取Linear、SWeibull和Logistic模型对微生物的失活过... 详细信息

为了探究微波处理对含乳固态成型制品中高风险微生物的致死效果,将大肠杆菌、沙门氏菌、金黄色葡萄球菌、黑曲霉以及毛霉5种微生物分别接种到乳基零食中,进而采用微波设备处理样品,选取Linear、SWeibull和Logistic模型对微生物的失活过程进行拟合,并采用相关系数(R^(2))、均方根误差(RMSE)、准确因子(A_(f))和偏差因子(B_(f))作为模型拟合效果的评判指标。结果表明,SWeibull和Logistic模型能够较好拟合微波处理后乳基零食中5种微生物的失活过程,R^(2)均在0.97及以上,A_(f)和B_(f)均在可接受范围内。研究可为微波杀菌技术在乳制品领域的应用提供参考。

关键词：微波杀菌乳基零食致病菌致死模型

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Gene-knockout by iSTOP enables rapid reproductive disease modeling and phenotyping in germ cells of the founder generation

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Science China(Life Sciences) 2024年第5期67卷 1035-1050页

作者： Yaling Wang Jingwen Chen Xueying Huang Bangguo Wu Peng Dai feng Zhang jinsong Li Lingbo Wang State Key Laboratory of Genetic Engineering Shanghai Key Laboratory of Metabolic Remodeling and HealthInstitute of Metabolism and Integrative BiologyFudan UniversityShanghai 200438China Institute of Reproduction and Development Obstetrics and Gynecology HospitalFudan UniversityShanghai 200011China NHC Key Lab of Reproduction Regulation(Shanghai Institute for Biomedical and Pharmaceutical Technologies) School of PharmacyFudan UniversityShanghai 200433China Shanghai Key Laboratoryof Maternal and Fetal Medicine Clinical and Translational Research Center of Shanghai First Maternity and Infant HospitalFrontier Science Center for Stem Cell ResearchSchoolof Life Sciences and TechnologyTongji UniversityShanghai 200092China State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China

Cytosine base editing achieves C·G-to-T·A substitutions and can convert four codons(CAA/CAG/CGA/TGG)into STOP-codons(induction of STOP-codons,iSTOP)to knock out genes with reduced *** enables direct phenotyping in founders’somatic cells,but it remains unknown whether this works in founders’germ cells so as to rapidly reveal novel genes for ***,we initially establish that iSTOP in mouse zygotes enables functional characterization of known genes in founders’germ cells:Cfap43-iSTOP male founders manifest expected sperm features resembling human“multiple morphological abnormalities of the flagella”syndrome(i.e.,MMAF-like features),while oocytes of Zp3-iSTOP female founders have no zona *** further illustrate iSTOP’s utility for dissecting the functions of unknown genes with Ccdc183,observing MMAF-like features and male infertility in Ccdc183-iSTOP founders,phenotypes concordant with those of Ccdc183-KO *** ultimately establish that CCDC183 is essential for sperm morphogenesis through regulating the assembly of outer dynein arms and participating in the intra-flagellar *** study demonstrates iSTOP as an efficient tool for direct reproductive disease modeling and phenotyping in germ cells of the founder generation,and rapidly reveals the essentiality of Ccdc183 in fertility,thus providing a time-saving approach for validating genetic defects(like nonsense mutations)for human infertility.

关键词： disease modeling infertility multiple morphological abnormalities of the flagella(MMAF) induction of STOP-codons(iSTOP CRISPR-STOP) sperm motility

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Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice

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Journal of Genetics and Genomics 2024年第4期51卷 407-418页

作者： Yaling Wang Xueying Huang Guoying Sun Jingwen Chen Bangguo Wu Jiahui Luo Shuyan Tang Peng Dai feng Zhang jinsong Li Lingbo Wang Shanghai Key Laboratory of Metabolic Remodeling and Health Institute of Metabolism and Integrative BiologyFudan UniversityShanghai 200438China Institute of Reproduction and Development Obstetrics and Gynecology HospitalFudan UniversityShanghai 200011China Shanghai Key Laboratory of Maternal and Fetal Medicine Clinical and Translational Research Center of Shanghai First Maternity and Infant HospitalFrontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China

During spermiogenesis,haploid spermatids undergo dramatic morphological changes to form slender sperm flagella and cap-like acrosomes,which are required for successful *** deformities in flagella cause a male infertility syndrome,multiple morphological abnormalities of the flagella(MMAF),while acrosomal hypoplasia in some cases leads to sub-optimal embryonic developmental ***,evidence regarding the occurrence of acrosomal hypoplasia in MMAF is ***,we report the generation of base-edited mice knocked out for coiled-coil domain-containing 38(Ccdc38)via inducing a nonsense mutation and find that the males are *** Ccdc38-KO sperm display acrosomal hypoplasia and typical MMAF *** find that the acrosomal membrane is loosely anchored to the nucleus and fibrous sheaths are disorganized in Ccdc38-KO *** analyses reveal that Ccdc38 knockout causes a decreased level of TEKT3,a protein associated with acrosome biogenesis,in testes and an aberrant distribution of TEKT3 in *** finally show that intracytoplasmic sperm injection overcomes Ccdc38-related *** study thus reveals a previously unknown role for CCDC38 in acrosome biogenesis and provides additional evidence for the occurrence of acrosomal hypoplasia in MMAF.

关键词： Acrosomal hypoplasia Multiple morphological abnormalities of the flagella(MMAF) Infertility Sperm motility Asthenoteratozoospermia Disease modeling

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makeTwin:A reference architecture for digital twin software platform

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Chinese Journal of Aeronautics 2024年第1期37卷 1-18页

作者： Fei TAO Xuemin SUN Jiangfeng CHENG Yonghuai ZHU Weiran LIU Yong WANG Hui XU Tianliang HU Xiaojun LIU Tingyu LIU Zheng SUN Jun XU jinsong BAO feng XIANG Xiaohui JIN Digital Twin International Research Center International Research Institute for Multidisciplinary ScienceBeihang UniversityBeijing 100191China School of Automation Science and Electrical Engineering Beihang UniversityBeijing 100191China Institute of Advanced Manufacturing and Intelligent Technology Beijing University of TechnologyBeijing 100124China RIAMB(Beijing)Technology Development Co Ltd(R.T.D.)Beijing 100120China School of Mechanical Engineering Shandong UniversityJinan 250061China School of Mechanical Engineering Southeast UniversityNanjing 211189China School of Mechanical Engineering Xi’an Jiaotong UniversityXi’an 710049China College of Mechanical Engineering Donghua UniversityShanghai 201620China School of Machinery and Automation Wuhan University of Science and TechnologyWuhan 430081China Beijing Aero-top Hi-tech Co LtdBeijing 100176China

Digital twin is currently undergoing a significant transformation from the conceptual and theoretical research phase to the implementation and application ***,a universally adaptable research and development platform for digital twin is critically needed to meet the development ***,a publicly accessible simulation,testing,and validation platform which can support digital twin model building,data processing,algorithm design,configuration,etc.,is urgently required for ***,for developers from the industry,a lowcode development platform that can offer a customizable suite of functions such as model creation,data management,protocol configuration,and visualization is urgently ***,for enterprise users,there is a lack of an application management platform that can be configured and migrated for various application scenarios,functions,and ***,based on the system research of digital twin theories and key technologies by the authors(such as the five-dimension digital twin model,digital twin modeling and digital twin data theory,digital twin standards,and so on),a digital twin software platform reference architecture,namely make Twin,is proposed and designed,as well as its ten core *** workflow of the make Twin and the interaction mechanisms among its core functions are ***,a digital twin application system for a chemical fiber textile shop floor(CFTS)which was developed according to make Twin,is introduced,which validates the proposed reference architecture.

关键词： Digital twin Digital twin software platform Industrial software makeTwin Reference architecture

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Quantized anomalous Hall resistivity achieved in molecular beam epitaxy-grown Mn Bi2Te4 thin films

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National Science Review 2024年第2期11卷 24-32页

作者： Yunhe Bai Yuanzhao Li Jianli Luan Ruixuan Liu Wenyu Song Yang Chen Peng-Fei Ji Qinghua Zhang Fanqi Meng Bingbing Tong Lin Li Yuying Jiang Zongwei Gao Lin Gu jinsong Zhang Yayu Wang Qi-Kun Xue Ke He Yang feng Xiao feng State Key Laboratory of Low Dimensional Quantum Physics Department of Physics Tsinghua University Institute of Physics Chinese Academy of Sciences School of Materials Science and Engineering Tsinghua University Beijing Academy of Quantum Information Sciences Frontier Science Center for Quantum Information Hefei National Laboratory Southern University of Science and Technology

The intrinsic magnetic topological insulator MnBi2Te4provides a feasible pathway to the high-temperature quantum anomalous Hall（QAH） effect as well as various novel topological quantum phases. Although quantized transport properties have been observed in exfoliated MnBi2Te4thin flakes, it remains a big challenge to achieve molecular beam epitaxy（MBE）-grown MnBi2Te4thin films even close to the quantized regime. In this work, we report the realization of quantized anomalous Hall resistivity in MBE-grown MnBi2Te4thin films with the chemical potential tuned by both controlled in situ oxygen exposure and top gating. We find that elongated post-annealing obviously elevates the temperature to achieve quantization of the Hall resistivity, but also increases the residual longitudinal resistivity, indicating a picture of high-quality QAH puddles weakly coupled by tunnel barriers. These results help to clarify the puzzles in previous experimental studies on MnBi2Te4and to find a way out of the big difficulty in obtaining MnBi2Te4samples showing quantized transport properties.

关键词： intrinsic magnetic topological insulator quantized anomalous Hall resistivity molecular beam epitaxy quantized transport property

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