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Tissue-resident macrophages exacerbate lung injury after remote sterile damage

Cellular & Molecular Immunology 2024年第4期21卷 332-348页

作者： Hanhui Zhong Jingjing Ji jinling zhuang Ziying Xiong Pengyun Xie Xiaolei Liu Jundi Zheng Wangli Tian Xiaoyang Hong Jing Tang The Department of Anesthesiology Affiliated Hospital of Guangdong Medical UniversityZhanjiangGuangdongChina The Department of Critical Care Medicine General Hospital of Southern Theater Command of PLAGuangzhouChina The Department of Respiratory Medicine Guangdong Provincial Hospital of Integrated Chinese and Western MedicineFoshanChina Pediatric Intensive Care Unit Senior Department of Pediatricsthe Seventh Medical Center of PLA General HospitalBeijingChina

Remote organ injury,which is a common secondary complication of sterile tissue damage,is a major cause of poor prognosis and is difficult to ***,we report the critical role of tissue-resident macrophages in lung injury after trauma or stroke through the inflammatory *** found that depleting tissue-resident macrophages rather than disrupting the recruitment of monocyte-derived macrophages attenuated lung injury after trauma or *** findings revealed that the release of circulating alarmins from sites of distant sterile tissue damage triggered an inflammatory response in lung-resident macrophages by binding to receptor for advanced glycation end products(RAGE)on the membrane,which activated epidermal growth factor receptor(EGFR).Mechanistically,ligand-activated RAGE triggered EGFR activation through an interaction,leading to Rab5-mediated RAGE internalization and EGFR phosphorylation,which subsequently recruited and activated P38;this,in turn,promoted RAGE translation and trafficking to the plasma membrane to increase the cellular response to RAGE ligands,consequently exacerbating *** study also showed that the loss of RAGE or EGFR expression by adoptive transfer of macrophages,blocking the function of RAGE with a neutralizing antibody,or pharmacological inhibition of EGFR activation in macrophages could protect against trauma-or stroke-induced remote lung ***,our study revealed that targeting the RAGE-EGFR signaling pathway in tissue-resident macrophages is a potential therapeutic approach for treating secondary complications of sterile damage.

关键词： tissue-resident macrophage RAGE remote sterile damage

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Saponin Ⅰ from Shuitianqi(Rhizoma Schizocapasae Plantagineae) inhibits metastasis by negatively regulating the transforming growth factor-β1/Smad7 network and epithelial-mesenchymal transition in the intrahepatic metastasis Bagg's Albino/c mouse model

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Journal of Traditional Chinese Medicine 2024年第4期44卷 642-651页

作者： LYU Meixian ZHOU Huan ZHI Limin ZHOU jinling GAN Rizhi QIN Yanping HE Nengting ZUO Qiqi LI Hao DONG Min LIANG Gang Pharmaceutical College Guangxi Medical UniversityNanning 530022China Department of Pharmacy Affiliated Tumor Hospital of Guangxi Medical UniversityNanning 530022China Drug clinical trial Facility office the People's Hospital of Guangxi Zhuang Autonomous RegionNanning 530022China College of Pharmacy Guangxi University of Chinese MedicineNanning 530022China Department of Pharmacy the Second Affiliated Hospital of Guangxi Medical UniversityNanning 530005China Department of Pharmacy People's Hospital of Guangxi HezhouHezhou 542899China Department of Pharmacy Guangzhou Women and Children's Medical Center Liuzhou HospitalLiuzhou 545000China Department of Pharmacy First Affiliated Hospital of Gannan Medical UniversityGannan 341000China

OBJECTIVE:To examine the influence of SaponinⅠfrom Shuitianqi(Rhizoma Schizocapasae Plantagineae)(SSPHⅠ)on hepatocellular carcinoma(HCC)metastasis,and to elucidate the underlying ***:The intrahepatic metastasis Bagg's Albino/c(BALB/c)mouse model was established with human hepatocellular carcinomas(HepG2)cells,then treated with normal saline(once per day),cisplatin(2 mg/kg,once every 2 d),and SSPHⅠ(25,50,and 75 mg/kg,once per day).Then,we assessed alterations in the hepatic pathology and target protein expressions in the intrahepatic metastasis BALB/c mouse model using a series of molecular biology ***:Based on our analysis,SSPHⅠsignificantly alleviated hepatocyte necrosis and tumor cells ***,SSPHⅠsuppressed extracellular matrix(ECM)degradation and angiogenesis via a decrease in matrix etalloproteinase-2(MMP-2),MMP-9,CD31,CD34,and vascular endothelial growth factor(VEGF)***,SSPHⅠrepressed invasion and metastasis by suppressing the transforming growth factor-β1(TGF-β1)/Smad7 axis and epithelial-mesenchymal transition(EMT),as evidenced by the scarce TGF-β1,Ncadherin,and Vimentin expressions,and elevated Smad7 and E-cadherin ***:The SSPHⅠ-mediated negative regulation of the TGF-β1/Smad7 axis and EMT are critical for the inhibition of HCC invasion and metastasis.

关键词： carcinoma,hepatocellular epithelial-mesenchymal transition transforming growth factor beta1 Smad7 protein Saponin I from Shuitianqi(Rhizoma Schizocapasae Plantagineae)

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协同护理模式干预在慢性伤口患者湿性疗法中的应用

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智慧健康 2023年第8期9卷 237-240页

作者：于凤英庄金玲李翠萍潍坊市坊子区人民医院山东潍坊261200

目的分析慢性伤口湿性疗法患者应用协同护理模式干预的效果。方法选择2019年4月-2021年2月本院收治的70例慢性伤口患者作为研究对象,两组均给予湿性疗法,通过电脑产生随机数分组方法将其分成对比组(实施常规护理)和研究组(开展协同护... 详细信息

目的分析慢性伤口湿性疗法患者应用协同护理模式干预的效果。方法选择2019年4月-2021年2月本院收治的70例慢性伤口患者作为研究对象,两组均给予湿性疗法,通过电脑产生随机数分组方法将其分成对比组(实施常规护理)和研究组(开展协同护理模式干预),各35例。对比两组伤口愈合情况、护理效果、自我效能。结果研究组干预后伤口愈合时间(32.79±4.62)d、PUSH评分(10.13±1.15)分、VAS评分(0.98±0.25)分均明显低于对比组(P0.05);干预后,研究组患者症状管理自我效能(7.33±1.04)分和疾病共性自我效能(7.95±1.17)分均明显高于对比组(P<0.05)。结论协同护理模式干预能有效缩短患者伤口愈合时间,减轻疼痛感受,改善伤口愈合程度,增加患者自我效能感,提高护理总有效率,具备较高的应用价值。

关键词：慢性伤口协同护理模式干预湿性疗法自我效能

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A Swin Transformer and Residualnetwork Combined Model for Breast Cancer Disease Multi-Classification Using Histopathological Images

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Instrumentation 2024年第1期11卷 112-120页

作者： Jianjun zhuang Xiaohui Wu Dongdong Meng Shenghua Jing School of Electronic and Information Engineering Nanjing University of Information Science and TechnologyNanJing 210044China Institute for AI in Medicine School of Artificial IntelligenceNanjing University of Information Science and TechnologyNanjing 210044China Department of Radiation Oncology Jinling HospitalSchool of Medicine Nanjing UniversityNanjing 210002China

Breast cancer has become a killer of women's health *** order to exploit the potential representational capabilities of the models more comprehensively,we propose a multi-model fusion ***,we combine two differently structured deep learning models,ResNet101 and Swin Transformer(SwinT),with the addition of the Convolutional Block Attention Module(CBAM)attention mechanism,which makes full use of SwinT's global context information modeling ability and ResNet101's local feature extraction ability,and additionally the cross entropy loss function is replaced by the focus loss function to solve the problem of unbalanced allocation of breast cancer data *** multi-classification recognition accuracies of the proposed fusion model under 40X,100X,200X and 400X BreakHis datasets are 97.50%,96.60%,96.30 and 96.10%,*** with a single SwinT model and ResNet 101 model,the fusion model has higher accuracy and better generalization ability,which provides a more effective method for screening,diagnosis and pathological classification of female breast cancer.

关键词： breast cancer pathological image swin transformer ResNet101 focal loss

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儿童癫痫持续状态的临床特点及脑电图分析

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中国卫生标准管理 2020年第7期11卷 62-66页

作者：方润桃庄锦玲杨燕珍朱少波周火旺福建医科大学附属漳州市医院儿科福建漳州363000

目的分析2019年1月—12月本院住院的97例儿童癫痫持续状态的临床特点及脑电图表现。方法对97例癫痫持续状态患儿的临床表现、颅脑磁共振及视频脑电图结果进行总结及回顾分析。结果儿童癫痫持续状态最常见于3岁以下儿童,其中64%为颅内外... 详细信息

目的分析2019年1月—12月本院住院的97例儿童癫痫持续状态的临床特点及脑电图表现。方法对97例癫痫持续状态患儿的临床表现、颅脑磁共振及视频脑电图结果进行总结及回顾分析。结果儿童癫痫持续状态最常见于3岁以下儿童,其中64%为颅内外感染,22%为癫痫,9%为遗传代谢病;多数患儿颅脑磁共振检查正常,部分可有异常信号及结构异常等表现;热性惊厥所致癫痫持续状态视频脑电图多数正常,颅内感染及脓毒性脑病引起癫痫持续状态视频脑电图多见局灶或弥漫性慢波表现,且脑电图异常程度与病情严重及颅脑磁共振异常呈正相关;癫痫患儿脑电图多表现为局灶棘波、尖波或慢波,伴颅内结构异常者脑电图异常波愈明显。结论儿童癫痫持续状态病因以感染引起最常见,脑电图异常程度与病情严重及颅脑磁共振异常呈正相关,对患儿行视频脑电图检查有助于协助病情评估,对指导患儿诊疗起积极作用。

关键词：儿童癫痫持续状态颅脑磁共振脑电图分析临床特点

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Updated overall survival and circulating tumor DNA analysis of ensartinib for crizotinib-refractory ALK-positive NSCLC from a phase II study

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Cancer Communications 2024年第4期44卷 455-468页

作者： Jing Zheng Tao Wang Yunpeng Yang Jie Huang Jifeng Feng Wu zhuang Jianhua Chen Jun Zhao Wei Zhong Yanqiu Zhao Yiping Zhang Yong Song Yi Hu zhuang Yu Youling Gong Yuan Chen Feng Ye Shucai Zhang Lejie Cao Yun Fan Gang Wu Yubiao Guo Chengzhi Zhou Kewei Ma Jian Fang Weineng Feng Yunpeng Liu Zhendong Zheng Gaofeng Li Huijie Wang Shundong Cang Ning Wu Wei Song Xiaoqing Liu Shijun Zhao Lieming Ding Giovanni Selvaggi Yang Wang Shanshan Xiao Qian Wang Zhilin Shen Jianya Zhou Jianying Zhou Li Zhang Department of Respiratory Disease Thoracic Disease CenterThe First Affiliated HospitalCollege of MedicineZhejiang UniversityZhejiang Provincial Clinical Research Center for Respiratory DiseaseHangzhouZhejiangP.R.China Hangzhou Repugene Technology Co. LtdHangzhouZhejiangP.R.China Department of Medical Oncology Sun Yat-sen University Cancer Cen-terState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdongP.R.China Department of Medical Oncology Jiangsu Cancer HospitalJiangsu Institute of Cancer ResearchThe Affiliated Cancer Hospital of Nanjing Medical UniversityNanjingJiangsuP.R.China Department of Thoracic Oncology Fujian Provincial Cancer HospitalFujian Medical University Cancer HospitalFuzhouFujianP.R.China Department of Medical Oncology-Chest Hunan Cancer HospitalChangshaHunanP.R.China Department of Thoracic Oncology Beijing Cancer HospitalBeijingP.R.China Department of Pulmonary Medicine Peking Union Medical College HospitalChinese Academy of Medical SciencesPeking Union Medical CollegeBeijingP.R.China Respiratory Department of Internal Medicine Henan Provincial Can-cer HospitalAffiliated Cancer Hospital of Zhengzhou UniversityZhengzhouHenanP.R.China Thoracic Medical Oncology Zhejiang Cancer HospitalHangzhouZhejiangP.R.China Division of Respiratory Medicine Jinling HospitalNanjing University School of MedicineNanjingJiangsuP.R.China Department of Oncology Chinese People’s Liberation Army(PLA)General HospitalBeijingP.R.China Department of Oncology The Affiliated Hospital of Qingdao UniversityQingdaoShandongP.R.China Department of Thoracic Oncology Cancer CenterWest China HospitalSichuan UniversityChengduSichuanP.R.China Department of Oncology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiP.R.China Department of Medical Oncology Cancer HospitalThe First Affiliated Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityTeaching Hospital of

Background:The initial phase II stuty(NCT03215693)demonstrated that ensartinib has shown clinical activity in patients with advanced crizotinib-refractory,anaplastic lymphoma kinase(ALK)-positive non-small cell lung cancer(NSCLC).Herein,we reported the updated data on overall survival(OS)and molecular profiling from the initial phase Ⅱ ***:In this study,180 patients received 225 mg of ensartinib orally once daily until disease progression,death or *** was estimated by Kaplan‒Meier methods with two-sided 95%confidence intervals(CIs).Next-generation sequencing was employed to explore prognostic biomarkers based on plasma samples collected at baseline and after initiating *** tumor DNA(ctDNA)was detected to dynamically monitor the genomic alterna-tions during treatment and indicate the existence of molecular residual disease,facilitating improvement of clinical ***:At the data cut-off date(August 31,2022),with a median follow-up time of 53.2 months,97 of 180(53.9%)patients had *** median OS was 42.8 months(95%CI:29.3-53.2 months).A total of 333 plasma samples from 168 patients were included for ctDNA *** inferior OS correlated sig-nificantly with baseline ALK or tumor protein 53(TP53)*** addition,patients with concurrent TP53 mutations had shorter OS than those without con-current TP53 *** ctDNA levels evaluated by variant allele frequency(VAF)and haploid genome equivalents per milliliter of plasma(hGE/mL)at baseline were associated with poor ***,patients with ctDNA clear-ance at 6 weeks and slow ascent growth had dramatically longer OS than those with ctDNA residual and fast ascent growth,***,patients who had a lower tumor burden,as evaluated by the diameter of target lesions,had a longer *** Cox regression analysis further uncovered the independent prognostic values of bone metastases,higher hGE,and elevated ALK mutation abundance at 6 we

关键词： anaplastic lymphoma kinase ctDNA ensartinib non-small cell lung cancer overall survival

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mTOR抑制剂对癫痫大鼠免疫微环境的影响

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中国医药指南 2023年第17期21卷 72-74页

作者：谢扑松何金水周火旺方润桃庄锦玲温洁芳杨妙雄朱少波福建医科大学附属漳州市医院福建漳州363000

目的探讨mTOR抑制剂对癫痫大鼠血液和脑组织免疫微环境的影响。方法选用40只癫痫大鼠,随机分为4组,每组10只,空白对照组(生理盐水干预),不同剂量mT OR抑制剂(雷帕霉素)干预组:低剂量组[1 mg/(kg·d)],中剂量组[2 mg/(kg·d)],高剂量组[4... 详细信息

目的探讨mTOR抑制剂对癫痫大鼠血液和脑组织免疫微环境的影响。方法选用40只癫痫大鼠,随机分为4组,每组10只,空白对照组(生理盐水干预),不同剂量mT OR抑制剂(雷帕霉素)干预组:低剂量组[1 mg/(kg·d)],中剂量组[2 mg/(kg·d)],高剂量组[4 mg/(kg·d)]。分别进行为期8周的干预,8周后处死大鼠,保留大鼠血清及脑组织样本。经ELISA法检测大鼠血清IL-2、IL-10和IFN-γ水平。经流式细胞技术检测大鼠脑组织的T细胞亚群。结果癫痫大鼠外周血IL-2、IL-10表达差异无统计学意义(P>0.05),IFN-γ表达差异有统计学意义(P<0.05)。脑组织中T淋巴细胞表达差异有统计学意义(P<0.05),随着给药剂量增加,T淋巴细胞含量有降低趋势,但始终高于空白组。结论雷帕霉素对癫痫大鼠的免疫微环境有调节作用,对脑组织起保护作用。

关键词：癫痫 mTOR抑制剂大鼠免疫微环境

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HK2和VDAC1在二乙酰吗啡致心肌细胞凋亡中的作用

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昆明医科大学学报 2024年第2期45卷 7-13页

作者：肖锦玲管雅玲朱森森庄梦婕苏丽萍蒲红伟新疆医科大学基础医学院病理学教研室新疆维吾尔自治区乌鲁木齐830011 新疆医科大学第一附属医院病理科新疆维吾尔自治区乌鲁木齐830011 新疆医科大学第一附属医院学科建设科新疆维吾尔自治区乌鲁木齐830011

目的探讨HK2和VDAC1在二乙酰吗啡致心肌细胞凋亡中的作用。方法采用剂量递增方法,建立二乙酰吗啡成瘾大鼠模型。将40只SD大鼠随机分为3组,正常组(Control,n=10)皮下注射等量生理盐水;模型组(Model,n=15)首次皮下注射二乙酰吗啡剂量为5 m... 详细信息

目的探讨HK2和VDAC1在二乙酰吗啡致心肌细胞凋亡中的作用。方法采用剂量递增方法,建立二乙酰吗啡成瘾大鼠模型。将40只SD大鼠随机分为3组,正常组(Control,n=10)皮下注射等量生理盐水;模型组(Model,n=15)首次皮下注射二乙酰吗啡剂量为5 mg/kg,以后逐日递增剂量2.5 mg/(kg·d),连续注射20 d;模型+10 D组(Model+10D,n=15)在模型组基础上继续递增剂量至第10天。采用ELISA法检测乳酸脱氢酶(lactate dehydrogenase,LDH)、谷草转氨酶(glutamic oxaloacetic transaminase,GOT)含量;采用HE染色观察各组心肌组织病理变化;采用TUNEL染色检测各组心肌组织细胞凋亡;采用免疫组化、RT-qPCR和Western blot检测HK2、VDAC1及凋亡相关因子的mRNA和蛋白的表达变化。结果HE染色发现随着二乙酰吗啡干预时间的延长,心肌组织表现出不同程度的损伤。与正常组相比,模型组中血清LDH、GOT含量及心肌凋亡率升高,HK2和抗凋亡因子Bcl-2的mRNA和蛋白水平下降,VDAC1和促凋亡因子Bax、Caspase-3的mRNA和蛋白水平升高,Clevead Caspase-3蛋白水平升高;与正常组相比,模型+10 D组中以上指标,差异有统计学意义(P<0.05)。结论二乙酰吗啡可导致心肌细胞凋亡,HK2和VDAC1可能参与了二乙酰吗啡致心肌细胞凋亡的过程。

关键词：二乙酰吗啡心肌凋亡己糖激酶2 电压依赖性阴离子通道1 裂解半胱氨酸天冬氨酸蛋白酶-3

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麦粒灸对环磷酰胺诱导的免疫抑制小鼠Th1/Th2平衡及转录因子T-bet、GATA3的影响

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北京中医药大学学报 2024年第6期47卷 818-825页

作者：朱涛王祯芝任佳程艳婷高珍冀雨芳苗晋玲冀来喜成都中医药大学针灸推拿学院成都611137 山西中医药大学第二临床学院山西省针灸医院针灸四科

目的观察麦粒灸对化学治疗诱导的免疫抑制小鼠Th1细胞(Th1)/Th2细胞(Th2)平衡及转录因子T盒子转录因子(T-bet)、GATA结合蛋白3(GATA3)的影响。方法32只SPF级雄性CD-1(ICR)小鼠按随机数字表法分为正常组、模型组、盐酸左旋咪唑组、麦粒灸... 详细信息

目的观察麦粒灸对化学治疗诱导的免疫抑制小鼠Th1细胞(Th1)/Th2细胞(Th2)平衡及转录因子T盒子转录因子(T-bet)、GATA结合蛋白3(GATA3)的影响。方法32只SPF级雄性CD-1(ICR)小鼠按随机数字表法分为正常组、模型组、盐酸左旋咪唑组、麦粒灸组,每组8只。除正常组外,其他各组小鼠腹腔注射环磷酰胺(80 mg/kg,每日1次,连续3 d)制备免疫抑制小鼠模型。盐酸左旋咪唑组小鼠灌胃盐酸左旋咪唑溶液(10 mg/kg);麦粒灸组小鼠灸“关元”、双侧“足三里”、双侧“三阴交”,每穴3壮,每壮约30 s。每日1次,连续7 d。观察小鼠一般情况;检测脾脏质量及脾脏指数;HE染色法观察脾脏组织病理;蛋白质印迹法和实时荧光PCR法检测小鼠脾脏组织T-bet、GATA3、γ干扰素(IFN-γ)、白细胞介素(IL)-4蛋白及mRNA表达;酶联免疫吸附测定法检测小鼠血清IFN-γ、IL-2、IL-4含量。结果与正常组比较,模型组小鼠反应迟钝、步态不稳;脾脏质量及脾脏指数增加(均P<0.05);脾脏组织结构紊乱,脾脏组织T-bet、IFN-γmRNA及蛋白表达下降,GATA3、IL-4 mRNA及蛋白表达升高(均P<0.05);血清IFN-γ、IL-2含量减少,IL-4含量增加(均P<0.05)。与模型组比较,盐酸左旋咪唑组和麦粒灸组小鼠一般情况好转,脾脏组织结构改善,脾脏组织T-bet、IFN-γmRNA及蛋白表达下降,GATA3、IL-4 mRNA及蛋白表达升高(均P<0.05);血清IFN-γ、IL-2含量减少,IL-4含量增加(均P<0.05)。结论麦粒灸对化学治疗诱导的免疫抑制症状具有改善作用,其机制可能是调节转录因子T-bet、GATA3的表达,调节Th1/Th2平衡,从而恢复免疫平衡状态。

关键词：麦粒灸环磷酰胺免疫抑制 Th1细胞/Th2细胞平衡 T盒子转录因子 GATA结合蛋白3 小鼠

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石墨相氮化碳二维纳米片载洋川芎内酯Ⅰ应用于缺血性脑卒中大鼠的治疗研究

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广西医科大学学报 2024年第6期41卷 833-840页

作者：杨航李金玲刘斯佳广西医科大学基础医学院广西脑科学研究重点实验室广西高校生物分子医学研究重点实验室南宁530021 广西医科大学再生医学与医用生物资源开发应用省部共建协同创新中心长寿与老年相关疾病教育部重点实验室广西再生医学重点实验室南宁530021

目的:探讨石墨相氮化碳(g-C_(3)N_(4))二维纳米片负载洋川芎内酯I(SEI)对缺血性脑卒中大鼠的治疗作用。方法:利用三聚氰胺通过热相反应生成层状g-C_(3)N_(4),热剥酸剥后得到薄层g-C_(3)N_(4)纳米片并进行表征;将SEI负载到g-C_(3)N_(4)... 详细信息

目的:探讨石墨相氮化碳(g-C_(3)N_(4))二维纳米片负载洋川芎内酯I(SEI)对缺血性脑卒中大鼠的治疗作用。方法:利用三聚氰胺通过热相反应生成层状g-C_(3)N_(4),热剥酸剥后得到薄层g-C_(3)N_(4)纳米片并进行表征;将SEI负载到g-C_(3)N_(4)纳米片上,通过紫外可见吸收光谱测定上清中的SEI,计算g-C_(3)N_(4)负载SEI质量比,测定SEI-g-C_(3)N_(4)复合纳米片在1 h、3 h、6 h、12 h、24 h和48 h的药物释放情况;SD大鼠大脑中动脉闭塞(MCAO)造模前48 h通过脑立体定位注射SEI-g-C_(3)N_(4)复合纳米片,造模后24 h进行神经行为学评分,评分结束后取材分别进行TTC染色、TUNEL染色和HE染色。结果:通过表征实验,制备的g-C_(3)N_(4)纳米片尺寸小(约100 nm)、比表面积大、分散性好、表面带正电荷(33.3 mV);紫外可见吸收光谱测定结果显示,g-C_(3)N_(4)已成功负载SEI,且SEI/g-C_(3)N_(4)质量比为15.06;药物释放结果显示,24 h时SEI的释放率接近50%;与sham组相比,MCAO组神经功能缺失评分显著升高(P0.05),MCAO+SEI-g-C_(3)N_(4)组神经功能缺失评分减少(P0.05);与MCAO组相比,MCAO+SEI-g-C_(3)N_(4)组凋亡率减少(P<0.001);体内生物安全性实验结果表明,与sham组相比,SEI组、SEI-g-C_(3)N_(4)组大鼠体重无统计学差异,且皮层脑组织结构和细胞形态无明显病理性变化。结论:成功制备了生物安全性较好的SEI-g-C_(3)N_(4)复合纳米片,且其能够减少MCAO大鼠的脑梗死面积,抑制细胞凋亡,减轻脑组织病理性变化,并且能改善大鼠的神经行为功能,表明SEI-g-C_(3)N_(4)复合纳米片可用于治疗缺血性脑卒中大鼠。

关键词：石墨相氮化碳洋川芎内酯Ⅰ 缺血性脑卒中大鼠

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