检索结果-南通市图书馆

Transcriptome sequencing assisted discovery and computational analysis of novel SNPs associated with flowering in Raphanus sativus in-bred lines for marker-assisted backcross breeding

引用

Horticulture Research 2019年第1期6卷 248-259页

作者： jinhee kim Abinaya Manivannan Do-Sun kim Eun-Su Lee Hye-Eun Lee Vegetable Research Division National Institute of Horticultural and Herbal ScienceRural Development AdministrationJeonju 55365Republic of Korea

The sequencing of radish genome aids in the better understanding and tailoring of traits associated with economic *** order to accelerate the genomics assisted breeding and genetic selection,transcriptomes of 33 radish inbred lines with diverse traits were sequenced for the development of single nucleotide polymorphic(SNP)*** sequence reads ranged from 2,560,543,741 bp to 20,039,688,139 bp with the GC(%)of 47.80–49.34 and phred quality score(Q30)of 96.47–97.54%.A total of 4951 polymorphic SNPs were identified among the accessions after stringent filtering and 298 SNPs with efficient marker assisted backcross breeding(MAB)markers were generated from the polymorphic ***,functional annotations of SNPs revealed the effects and importance of the SNPs identified in the flowering *** SNPs were predominantly associated with the four major flowering related transcription factors such as MYB,MADS box(AG),AP2/EREB,and *** addition,SNPs in the vital flowering integrator gene(FT)and floral repressors(EMBRYONIC FLOWER 1,2,and FRIGIDA)were identified among the radish inbred ***,50 SNPs were randomly selected from 298 SNPs and validated using Kompetitive Allele Specific PCR genotyping system(KASP)in 102 radish inbred *** homozygosity of the inbred lines varied from 56 to 96%and the phylogenetic analysis resulted in the clustering of inbred lines into three *** together,the SNP markers identified in the present study can be utilized for the discrimination,seed purity test,and adjusting parental combinations for breeding in radish.

关键词： breeding Raphanus utilized

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Melatonin combined with exercise cannot alleviate cerebral injury in a rat model of focal cerebral ischemia/reperfusion injury

引用

Neural Regeneration Research 2012年第13期7卷 993-999页

作者： Seunghoon Lee jinhee Shin Minkyung Lee Yunkyung Hong Sang-Kil Lee Youngjeon Lee Tserentogtokh Lkhagvasuren Dong-Wook kim Young-Ae Yang Kyu-Tae Chang Yonggeun Hong Department of Rehabilitation Science in Interdisciplinary Ph.D.Program Inje UniversityGimhae 621-749Republic of Korea Cardiovascular & Metabolic Disease Center College of Biomedical Science & EngineeringGimhae 621-749Republic of Korea Department of Smart Foods & Drugs School of Food & Life SciencesInje UniversityGimhae 621-749Republic of Korea Department of Pharmaceutical Engineering College of EngineeringInje UniversityGimhae 621-749Republic of Korea National Primate Research Center Korea Research Institute of BiotechnologyOchang 363-883Republic of Korea

Previous studies have demonstrated that melatonin combined with exercise can alleviate secondary damage after spinal cord injury in rats. Therefore, it is hypothesized that melatonin combined with exercise can also alleviate ischemic brain damage. In this study, adult rats were subjected to right middle cerebral artery occlusion after receiving 10 mg/kg melatonin or vehicle subcutaneously twice daily for 14 days. Forced exercise using an animal treadmill was performed at 20 m/min for 30 minutes per day for 6 days prior to middle cerebral artery occlusion. After middle cerebral artery occlusion, each rat received melatonin combined with exercise, melatonin or exercise alone equally for 7 days until sacrifice. Interestingly, rats receiving melatonin combined with exercise exhibited more severe neurological deficits than those receiving melatonin or exercise alone. Hypoxia-inducible factor la mRNA in the brain tissue was upregulated in rats receiving melatonin combined with exercise. Similarly, microtubule associated protein-2 mRNA expression was significantly upregulated in rats receiving melatonin alone. Chondroitin sulfate proteoglycan 4 （NG2） mRNA expression was significantly decreased in rats receiving melatonin combined with exercise as well as in rats receiving exercise alone. Furthermore, neural cell loss in the primary motor cortex was significantly reduced in rats receiving melatonin or exercise alone, but the change was not observed in rats receiving melatonin combined with exercise. These findings suggest that excessive intervention with melatonin, exercise or their combination may lead to negative effects on ischemia/reperfusion-induced brain damage.

关键词： wfocal cerebral ischemiaJreperfusion melatonin exercise neurological function brain tissue loss microtubule associated protein-2 chondroitin sulfate proteoglycan 4 NG2 hypoxia-inducible factor1 alpha neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Predictors of Co-Occurring Substance Use among Asian Americans in Residential Treatment Programs

引用

Open Journal of Psychiatry 2019年第2期9卷 153-164页

作者： Minjeong kim jinhee Lee Midori Nakajima Linda Chafetz School of Nursing San Diego State University San Diego USA School of Nursing California State University-East Bay Hayward USA University of California-San Francisco San Francisco USA

Although severe and chronic mental disorders are common among Asian Americans in residential treatment programs, little has been known about the prevalence and predictors of co-occurring substance use in this population. The purpose of this study was to examine predictors of co-occurring substance use among Asian Americans with mental disorders in residential treatment programs. This cross-sectional study included 375 clinical records of Asian Americans from residential treatment programs between 2007 and 2011. Demographic variables, principal psychiatric diagnoses, and data on alcohol, stimulant, and marijuana use were obtained from the clinical records. Separate binary logistic regression analyses were used to examine the demographic and diagnostic contributions to the risk of each type of substance use. Findings of this study indicated that the prevalence of co-occurring substance use was about 53% in Asian Americans with mental disorders. Binary logistic regression analyses revealed that male gender, older age, and depressive disorder predicted more alcohol use, but homelessness and schizophrenia predicted less alcohol use. Male gender, homelessness, and smoking predicted more stimulant use. Male gender and younger age predicted more marijuana use. Based on the findings of this study, awareness about co-occurring substance use problems of ethnic minority psychiatric clients should be increased and appropriate substance use prevention and treatment programs should be developed and provided for high-risk groups.

关键词： Asian Americans Mental Disorders Substance Use

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Green tea extract and Piper retrofractum attenuate deoxycholic acid-induced damage and enhance the tight junction barrier: An analysis in a Caco-2 cell culture model and a DSS coinduced mouse model

引用

Food Bioscience 2023年第2期52卷 541-548页

作者： Kwanyong Choi Soo-yeon Park Yuri Kwon jinhee Lee Oran Kwon Ji Yeon kim Department of Food Science and Technology Seoul National University of Science and Technology232Gongneung-roNowon-guSeoul01811Republic of Korea Health Food Research and Development NEWTREE Co.Ltd.6-14Baekjegobun-ro 27-gilSognpa-guSeoul05604Republic of Korea Department of Nutritional Science and Food Management Ewha Womans University52 Ewhayeodae-gilSeodeamun-guSeoul03760Republic of Korea Department of Nano Bio Engineering Seoul National University of Science and Technology232Gongneung-roNowon-guSeoul01811Republic of Korea

This study investigated the effect of green tea extract(GTE)and green tea-Piper retrofractum complex(GPX),on tight junctions(TJs)in a cellular model of deoxycholic acid(DCA)-induced intestinal barrier disruption and a mouse DSS+DCA-induced colitis *** tea and *** were extracted and coadministered to examine the bioavailability and effects of combined catechins and piperine on colonic TJ *** ability of GPX to alleviate TJ damage was determined by comparing the transepithelial electrical resistance(TEER),FITC-dextran flux,and mRNA expression of TJ-related components and target expression pathway in the GTE and GPX *** with the DCA-treated group,the GTE-and GPX-treated groups showed significantly enhanced TEER,FITC-dextran flux,and TJ-related mRNA expression(p<0.05).Cell treatment also downregulated the mRNA encoding bile acid receptor and decreased ERK1/2 *** with the GTE-treated group,the GPX-treated group showed higher mRNA expression of zonula occludens-1,occludin,claudin-3,and claudin-4;greater restoration of TJs,as determined by immunofluorescence;and better modulation of ERK1/2 *** the in vivo model,GPX-pretreated mice showed an improved intestinal damage index and colon length compared with mice in the other colitis-induced *** extracts recovered the distal colon mRNA expression of zonula occludens-1 and *** results suggest that GTE improves intestinal health by protecting TJs from secondary bile acid damage and that *** may potentiate the bioactivity of green tea catechins.

关键词： Green tea and Piper retrofractum Catechin Piperine Deoxycholic acid Tight junction barrier

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

ACUPUNCTURE STIMULATION IMPROVES MEMORY IMPAIRMENT INDUCED BY SCOPOLAMINE IN RATS

引用

Journal of Integrative Medicine 2014年第3期 255-255页

作者： Bombi Lee Bongjun Sur jinhee Park Sung-Hun kim Mijung Yeom Insop Shim Hyejung Lee Dae-Hyun Hahm Kyung Hee University

Objective: The purpose of this study was to examine whether acupuncture stimulation at the Baihui(GV20) improves memory defects caused by administration of scopolamine(SCO) to the *** effects of acupuncture stimulation at the GV20 on the cholinergic system as well as the expression of brain-derived neurotrophic factor(BDNF) and cAMP-response element-binding protein(CREB) in the hippocampus were also ***: Male rats were administered with SCO(2 mg/kg, i.p.) once daily for 14 *** stimulation at GV20 was performed for 5 min before SCO ***: Acupuncture stimulation at GV20 improved memory impairment as measured by the passive avoidance test(PAT) and reduced the escape latency for finding the platform in the Morris water maze(MWM) *** stimulation at the GV20 significantly alleviated memory-associated decreases in the expression levels of choline acetyltransferase(ChAT), BDNF and CREB proteins in the ***, acupuncture stimulation at the GV20 significantly restored the expression of choline transporter 1(CHT1), vesicular acetylcholine transporter(VAChT), BDNF and CREB mRNAs in the ***: These results demonstrated that acupuncture stimulation at the GV20 has significant neuroprotective effects against neuronal impairment and memory dysfunction caused by SCO in ***, these findings suggested that acupuncture stimulation at the GV20 might be a useful therapy in various neurodegenerative diseases for the improvement of cognitive functioning via stimulating cholinergic enzyme activities and regulating BDNF and CREB expression in the brain.

关键词： CREB BDNF SCO ACUPUNCTURE STIMULATION IMPROVES MEMORY IMPAIRMENT INDUCED BY SCOPOLAMINE IN RATS

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：