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FeOOH-loaded mesoporous silica nanoparticles as a theranostic platform with pH-responsive MRI contrast enhancement and drug release

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Science China Chemistry 2018年第7期61卷 806-811页

作者： Guoming Huang Rui liu Yuehua Hu Shi-Hua li Ying Wu Yuan Qiu jingying li Huang-Hao Yang College of Biological Science and Engineering Fuzhou University Key Laboratory for Analytical Science of Food Safety and Biology Ministry of Education State Key Laboratory of Photocatalysis on Energy and Environment College of Chemistry Fuzhou University

The development of stimuli-responsive theranostic platforms is of great demand for efficient cancer treatment because they can enhance diagnostic specificity and *** this work,we report a p H-responsive theranostic nanoplatform based on Fe OOH clusters loaded mesoporous silica nanoparticles(Fe@MSNs).The as-synthesized Fe@MSNs possess activatable T_1magnetic resonance imaging(MRI)performance that can respond to the acidic microenvironment of solid tumor to turn on T_1singals by releasing paramagnetic Fe^(3+)*** Fe@MSNs are biocompatible without appreciable ***,the unique mesoporous structure endows the Fe@MSNs with significant advantages to effectively deliver chemotherapeutic drug for inhibiting the growth of solid *** believe that this novel p H-responsive theranostic nanoplatform holds great promise in cancer treatment.

关键词： mesoporous silica nanoparticles magnetic resonance imaging pH-response cancer diagnosis cancer therapy

Generation of Targeted Point Mutations in Rice by a Modified CRISPR/Cas9 System

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Molecular Plant 2017年第3期10卷 526-529页

作者： jingying li Yongwei Sun Jinlu Du Yunde Zhao Lanqin Xia Institute of Crop Sciences (ICS) Chinese Academy of Agricultural Sciences (CAAS) Beijing 100081 China National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University Wuhan 430070 China Section of Cell and Developmental Biology University of California San Diego La Jolla CA 92093-0116 USA These authors contributed equally to this article

CRISPR/Cas9 （Clustered Regularly Interspaced Short Palin- dromic Repeats/CRISPR-associated Cas9 endonuclease）- mediated genome editing has revolutionized biological research and crop improvement because of its speci... 详细信息

关键词： Targeted Point Mutations Modified CRISPR/Cas9 System Short Palindromic

X-ray sensitive high-Z metal nanocrystals for cancer imaging and therapy

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Nano Research 2021年第11期14卷 3744-3755页

作者： liting Zheng Rong Zhu Lanlan Chen Qinrui Fu jingying li Chen Chen Jibin Song Huanghao Yang MOE Key Laboratory for Analytical Science of Food Safety and Biology College of ChemistryFuzhou UniversityFuzhou350116China

Radiotherapy(RT)based on X-ray irradiation is a widely applied cancer treatment strategy in the ***,treating cancer based on RT alone usually results in insufficient radiation energy deposition,which inevitably has serious side effects on healthy parts of the ***,high atomic number(high-Z)metal nanocrystals as X-ray sensitizers can reduce the radiation dose effectively due to their high X-ray absorption,which has attracted increased attention in recent ***-Z metal nanocrystals produce Auger and photoelectrons electrons under X-ray irradiation,which could generate large amounts of reactive oxygen species,and induce cellular *** sensitization effect of high-Z metal nanocrystals is closely related with their composition,morphologies,and size,which would strongly impact their performances in the application of cancer imaging and *** this review,we summarize diverse types of X-ray sensitizers such as bismuth,hafnium,gold,and gadolinium for cancer RT and imaging *** addition,current challenges and the outlook of RT based on high-Z metal nanocrystals are also discussed.

关键词： radiotherapy X-ray high-Z metal nanocrystal cancer therapy

Artificial evolution of OsEPSPS through an improved dual cytosine and adenine base editor generated a novel allele conferring rice glyphosate tolerance

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Journal of Integrative Plant Biology 2023年第9期65卷 2194-2203页

作者： Chen Zhang Xue Zhong Shaoya li Lei Yan jingying li Yubing He Yong lin Yangjun Zhang Lanqin Xia Institute of Crop Sciences(ICS) Chinese Academy of Agricultural Sciences(CAAS)Beijing 100081China CAAS/Hainan Yazhou Bay Seed Laboratory National Nanfan Research Institute(Sanya)Sanya 572024China Key Laboratory of Gene Editing Technologies(Hainan) Ministry of Agricultural and Rural AffairsSanya 572024China Beijing Dabeinong Technology Group Co. LtdBeijing 10080China

Exploiting novel endogenous glyphosate-tolerant alleles is highly desirable and has promising potential for weed control in rice breeding. Here,through fusions of different effective cytosine and adenine deaminases with nCas9-NG, we engineered an effective surrogate two-component composite base editing system, STCBE-2, with improved C-to-T and A-to-G base editing efficiency and expanded the editing window. Furthermore,we targeted a rice endogenous OsEPSPS gene for artificial evolution through STCBE-2-mediated near-saturated mutagenesis. After hygromycin and glyphosate selection, we identified a novel OsEPSPS allele with an Asp-213-Asn(D213N)mutation(OsEPSPS-D213N) in the predicted glyphosate-binding domain, which conferred rice plants reliable glyphosate tolerance and had not been reported or applied in rice breeding. Collectively, we developed a novel dual base editor which will be valuable for artificial evolution of important genes in crops. And the novel glyphosate-tolerant rice germplasm generated in this study will benefit weeds management in rice paddy fields.

关键词： artificial evolution dual cytosine and adenine base editor nCas9‐NG rice(Oryza sativa L) near‐saturated mutagenesis

Multiplex precision gene editing by a surrogate prime editor in rice

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Molecular Plant 2022年第7期15卷 1077-1080页

作者： Huiyuan li Ziwei Zhu Shaoya li jingying li Lei Yan Chen Zhang Youzhi Ma Lanqin Xia Institute of Crop Sciences(ICS) Chinese Academy of Agricultural Sciences(CAAS)Be11ing 100081China Hainan Yazhou Bay Seed Laboratory/National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanyaHainan Province 572024China

Dear Editor,Development of a multiplex precision gene editing system is highly desirable for pyramiding beneficial alleles in crop *** editing is a newly developed genome-editing tool that can precisely enable the installation of all 12 nucleotide sub-stitutions,short insertions,and deletions without exogenous DNA donor repair template and double-strand breaks(Anzalone et al.,2019).Among the prime editing systems,prime editor 3(PE3),which consists of a prime editing guide RNA(pegRNA)and an additional nicking single guide RNA(sgRNA)to nick the non-edited strand,enhances editing efficiency.

关键词： editor editing template

Graphitic Carbon Quantum Dots Modified Nickel Cobalt Sulfide as Cathode Materials for Alkaline Aqueous Batteries

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Nano-Micro Letters 2020年第2期12卷 1-18页

作者： Yirong Zhu jingying li Xiaoru Yun Ganggang Zhao Peng Ge Guoqiang Zou Yong liu Hongshuai Hou Xiaobo Ji College of Metallurgy and Material Engineering Hunan University of TechnologyZhuzhou 412007People’s Republic of China College of Chemistry and Chemical Engineering Central South UniversityChangsha 410083People’s Republic of China State Key Lab of Powder Metallurgy Central South UniversityChangsha 410083People’s Republic of China

Carbon quantum dots(CQDs)as a new class of emerging materials have gradually drawn researchers’concern in recent *** this work,the graphitic CQDs are prepared through a scalable approach,achieving a high yield with more than 50%.The obtained CQDs are further used as structure-directing and conductive agents to synthesize novel N,S-CQDs/NiCo2S4 composite cathode materials,manifesting the enhanced electrochemical properties resulted from the synergistic effect of highly conductive N,S-codoped CQDs offering fast electronic transport and unique micro-/nanostructured NiCo2S4 microspheres with Faradaic redox characteristic contributing large ***,the nitrogen-doped reduced graphene oxide(N-rGO)/Fe2O3 composite anode materials exhibit ultrahigh specific capacity as well as significantly improved rate property and cycle performance originating from the high-capacity prism-like Fe2O3 hexahedrons tightly wrapped by highly conductive N-rGO.A novel alkaline aqueous battery assembled by these materials displays a specific energy(50.2 Wh kg^−1),ultrahigh specific power(9.7 kW kg^−1)and excellent cycling performance with 91.5%of capacity retention at 3 A g^−1 for 5000 *** present research offers a valuable guidance for the exploitation of advanced energy storage devices by the rational design and selection of battery/capacitive composite materials.

关键词： Energy storage Alkaline aqueous batteries Carbon quantum dot Nickel cobalt sulfide

Plant genome editing using xCas9 with expanded PAM compatibility

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Journal of Genetics and Genomics 2019年第5期46卷 277-280页

作者： jingying li Jinman Luo Meilian Xu Shaoya li Jiahui Zhang Huiyuan li Lei Yan Yunde Zhao Lanqin Xia Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing100081China National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research Huazhong Agricultural UniversityWuhan430070Chin Shaoya li Jiahui ZhangHuiyuan LiLei Yan Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing100081China Section of Cell and Developmental Biology University of CaliforniaSan DiegoLa JollaCA92093-0V6USA

CRISPR/Cas enables robust genome editing and has revolution-ized both functional genomics and crop *** specificity of Cas-directed DNA cleavage is strictly determined by a chimeric single guide RNA(SgRNA)and a short protospacer adjacent motif(PAM)in the genome(Cong et al,2013;Zetsche et al,2015).The widely used Cas9 from Streptococcus pyogenes(SpCas9)generally recognizes the canonical NGG PAM(where N indicates any nucleicacid base)(Miao et al.,2013;Ma et al,2015),making many regionsuntargetable by ***9 VQR and VRER variants,which recog-nize the non-canonical PAM sequences of NGA and NGCG,respectively,have been used to expand targetable sequences in plants(Hu et al.,2016).In addition,the applications of other Cas endonucleases such as SaCas9(Staphylococcus aureus Cas9)(Ran et al..2015).

关键词： Plant genome editing PAM compatibility

Engineering a plant A-to-K base editor with improved performance by fusion with a transactivation module

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Plant Communications 2023年第6期4卷 225-229页

作者： Yucai li Shaoya li Chenfei li Chen Zhang Lei Yan jingying li Yubing He Yan Guo Yong lin Yangjun Zhang Lanqin Xia Institute of Crop Sciences(ICS) Chinese Academy of Agricultural Sciences(CAAS)Beijing 100081China Hainan Yazhou Bay Seed Laboratory/National Nanfan Research Institute(Sanya) CAASSanyaHainan Province 572024China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Beijing Dabeinong Technology Group Co. Ltd.Beijing 10080China

Dear Editor,Base editors(BEs)are promising tools that enable single-nucleotide substitutions in specific target genes,generating loss-offunction or gain-of-function mutations,which can greatly accelerate plant functional genomics and crop improvement(Ren et al.,2018;Zeng et al.,2020;Xu et al.,2021;Yan et al.,2021;li et al.,2023).To date,three major classes of BEs have been engineered:cytosine BEs(CBEs)for C-to-T transitions(Komor et al.,2016),adenine BEs(ABEs)for A-to-G transitions(Gaudelli et al.,2017).

关键词： CBE editor base

Efficient allelic replacement in rice by gene editing： A case study of the NRT1.1B gene

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Journal of Integrative Plant Biology 2018年第7期60卷 536-540页

作者： jingying li Xin Zhang Yongwei Sun Jiahui Zhang Wenming Du Xiuping Guo Shaoya li Yunde Zhao Lanqin Xia Institute of Crop Sciences (ICS) Chinese Academy of Agricultural Sciences (CAAS)Beijing 100081 China National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Huazhong Agricultural UniversityWuhan 430070 China Section of Cell and Developmental Biology University of CaliforniaSan Diego La Jolla CA 92093‐0116 USA

Summary Precise replacement of an existing allele in commercial cultivars with an elite allele is a major goal in crop breeding. A single nucleotide polymorphism in the NRT1.1B gene between japonica and indica rice is responsible for the improved nitrogen use efficiency in indica rice. Herein, we precisely replaced the japonica NRT1.1B allele with the indica allele, in just one generation, using CRISPR/Cas9 gene-editing technology. No additional selective pressure was needed to enrich the precise replacement events.

关键词： HDR B gene Figure A case study of the NRT Efficient allelic replacement in rice by gene editing