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Generation of tooth-like structures from integration-free human urine induced pluripotent stem cells

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Cell Regeneration 2013年第1期2卷 42-49页

作者： jinglei cai Yanmei Zhang Pengfei Liu Shubin Chen Xuan Wu Yuhua Sun Ang Li Ke Huang Rongping Luo Lihui Wang Ying Liu Ting Zhou Shicheng Wei Guangjin Pan Duanqing Pei CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cell Biology and Regenerative Medicine South China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institute of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan AvenueScience ParkGuangzhou 510530P.R.China Department of Regeneration Medicine School of PharmaceuticalJilin UniversityChangchunP.R.China Department of Oral and Maxillofacial Surgery Laboratory of Interdisciplinary StudiesSchool and Hospital of StomatologyPeking UniversityBeijingP.R.China Biodynamic Optical Imaging Center(BIOPIC) Peking UniversityBeijingP.R.China The Shenzhen Key Lab of Gene and Antibody Therapy Center for Biotech&Biomedicine and Division of Life SciencesGraduate School at ShenzhenTsinghua UniversityShenzhenP.R.China Center for Biomedical Materials and Tissue Engineering Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijingP.R.China.

Background:Tooth is vital not only for a good smile,but also good ***,we lose tooth regularly due to accidents or *** ideal solution to this problem is to regenerate tooth with patients’own *** we describe the generation of tooth-like structures from integration-free human urine induced pluripotent stem cells(ifhU-iPSCs).Results:We first differentiated ifhU-iPSCs to epithelial sheets,which were then recombined with E14.5 mouse dental ***-like structures were recovered from these recombinants in 3 weeks with success rate up to 30%for 8 different iPSC lines,comparable to H1 *** further detected that ifhU-iPSC derived epithelial sheets differentiated into enamel-secreting ameloblasts in the tooth-like structures,possessing physical properties such as elastic modulus and hardness found in the regular human ***:Our results demonstrate that ifhU-iPSCs can be used to regenerate patient specific dental tissues or even tooth for further drug screening or regenerative therapies.

关键词： Human urine Integration-free iPSCs Recombinant tooth Bioengineered tooth Dental epithelium

Efficient induction of neural progenitor cells from human ESC/iPSCs on Type I Collagen

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Science China(Life Sciences) 2021年第12期64卷 2100-2113页

作者： Pengfei Liu Shubin Chen Yaofeng Wang Xiaoming Chen Yiping Guo Chunhua Liu Haitao Wang Yifan Zhao Di Wu Yongli Shan Jian Zhang Chuman Wu Dongwei Li Yanmei Zhang Tiancheng Zhou Yaoyu Chen Xiaobo Liu Chenxu Li Lihui Wang Bei Jia Jie Liu Bo Feng jinglei cai Duanqing Pei CAS Key Laboratory of Regenerative Biology Guangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Ambulatory Surgical Center The 2nd Clinical medical College(Shenzhen People’s Hospital)of Jinan UniversityThe 1st Affiliated Hospitals of Southern University of Science and TechnologyShenzhen 518020China Integrated Chinese and Western Medicine Postdoctoral Research Station Jinan UniversityGuangzhou 510632China Bioland Laboratory Guangzhou Regenerative Medicine and Health Guangdong LaboratoryGuangzhou 510005China School of Biomedical Sciences The Chinese University of Hong KongHong Kong SARChina Centre for Regenerative Medicine and Health Hong Kong Institute of Science&InnovationChinese Academy of SciencesHong Kong SARChina Department of Regenerative Medicine School of Pharmaceutical ScienceJilin UniversityChangchun 130012China Department of Pathology Medical CollegeJinan UniversityGuangzhou 510632China The Center for Prenatal and Hereditary Disease Diagnosis Department of Obstetrics and GynecologyNanfang HospitalSouthern Medical UniversityGuangzhou 510515China Department of Obstetrics and Gynecology Nanfang HospitalSouthern Medical UniversityGuangzhou 510515China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China

A stable,rapid and effective neural differentiation method is essential for the clinical applications of human embryonic stem cells(ESCs)or induced pluripotent stem cells(iPSCs)in treating neurological disorders and ***,we established a novel and robust monolayer differentiation method to produce functional neural progenitor cells(NPCs)from human ESC/iPSCs on Type I *** derived cells not only displayed the requisite markers,but also behaved similarly to classic NPCs both in vitro and in *** transplantation into traumatic brain injury model,the derived NPCs facilitated recovery from *** also found that SMAD signaling stayed down throughout the differentiation process on Type I Collagen,and the pluripotent signals were rapidly downregulated along with raising up of neural early markers on the third ***,ATAC-seq data showed the related mediation of distinct transcriptome and global chromatin dynamics during NPC ***,our results thus provide a convenient way to generate NPCs from human ESC/iPSCs for neural diseases’treatment.

关键词： ESCs iPSCs NPCs neural differentiation Type I Collagen

Magnetism Measurements of Two-Dimensional van der Waals Antiferromagnet CrPS_(4)Using Dynamic Cantilever Magnetometry

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Chinese Physics Letters 2024年第10期41卷 123-128页

作者： Qi Li Weili Zhen Ning Wang Meng Shi Yang Yu Senyang Pan Lin Deng Jiaqiang cai Kang Wang Lvkuan Zou Zhongming Zeng Zhaosheng Wang jinglei Zhang Anhui Province Key Laboratory of Condensed Matter Physics at Extreme Conditions High Magnetic Field LaboratoryHFIPSChinese Academy of SciencesHefei 230031China Science Island Branch of Graduate School University of Science and Technology of ChinaHefei 230026China School of Advanced Manufacturing Engineering Hefei UniversityHefei 230601China Suzhou Institute of Nano-tech and Nano-bionics Chinese Academy of SciencesSuzhou 215123China

Recent experimental and theoretical work has focused on two-dimensional van der Waals(2D vdW)magnets due to their potential applications in sensing and spintronics *** measurements of these emerging materials,conventional magnetometry often encounters challenges in characterizing the magnetic properties of small-sized vdW materials,especially for antiferromagnets with nearly compensated magnetic ***,we investigate the magnetism of 2D antiferromagnet CrPS_(4)with a thickness of 8nm by using dynamic cantilever magnetometry(DCM).

关键词： (4) magnets Magnet

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高职工程造价专业如何适应市场需求

邯郸职业技术学院学报 2004年第4期17卷 12-13页

作者：蔡晶磊邯郸职业技术学院建工系河北邯郸056001

社会经济多元化发展的格局决定了市场对人才需求也向多元化发展 ,高职教育应紧跟时代步伐 ,深化教育教学改革。适应调整人才培养规格和培养目标 ,探索一条适合高职特点的教育之路 ,以适应人才市场的需求。

关键词：高职教育适应专业步伐教育教学改革人才培养规格培养目标职工市场需求多元化发展

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如何在工程量清单招标模式下提高中标率

工程建设 2007年第2期39卷 50-52页

作者：蔡晶磊邯郸大学建工系河北邯郸056001

介绍了在工程量清单招标模式下,为提高中标率应重视的关键过程,报价策略和方法,并对该招标模式下尚存在的与市场竞争机制不相一致的若干问题进行了探讨。

关键词：工程量清单清单计价招投标中标率

GATA2^(−/−) human ESCs undergo attenuatedendothelial to hematopoietic transition andthereafter granulocyte commitment

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Cell Regeneration 2015年第1期4卷 32-46页

作者： Ke Huang Juan Du Ning Ma Jiajun Liu Pengfei Wu Xiaoya Dong Minghui Meng Wenqian Wang Xin Chen Xi Shi Qianyu Chen Zhongzhou Yang Shubin Chen Jian Zhang Yuhang Li Wei Li Yi Zheng jinglei cai Peng Li Xiaofang Sun Jinyong Wang Duanqing Pei Guangjin Pan CAS Key Laboratory of Regenerative Biology South China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine South China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Department of Hematology Sun Yat-sen UniversityGuangzhou 510630China South China University of Technology Guangzhou 510641China Key Laboratory for Major Obstetric Diseases of Guangdong Province GuangzhouChina Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes GuangzhouChina

Background: Hematopoiesis is a progressive process collectively controlled by an elaborate network of transcriptionfactors (TFs). Among these TFs, GATA2 has been implicated to be critical for regulating multiple steps of hematopoiesisin mouse models. However, whether similar function of GATA2 is conserved in human hematopoiesis, especially duringearly embryonic development stage, is largely ***: To examine the role of GATA2 in human background, we generated homozygous GATA2 knockout humanembryonic stem cells (GATA2^(−/−) hESCs) and analyzed their blood differentiation potential. Our results demonstratedthat GATA2^(−/−) hESCs displayed attenuated generation of CD34^(+)CD43^(+) hematopoietic progenitor cells (HPCs), due tothe impairment of endothelial to hematopoietic transition (EHT). Interestingly, GATA2^(−/−) hESCs retained the potentialto generate erythroblasts and macrophages, but never granulocytes. We further identified that SPI1 downregulationwas partially responsible for the defects of GATA2^(−/−) hESCs in generation of CD34^(+)CD43^(+) HPCs and ***, we found that GATA2^(−/−) hESCs restored the granulocyte potential in the presence of Notch ***: Our findings revealed the essential roles of GATA2 in EHT and granulocyte development throughregulating SPI1, and uncovered a role of Notch signaling in granulocyte generation during hematopoiesis modeled byhuman ESCs.

关键词： hESCs GATA2 EHT HPC Granulocyte Notch signaling

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干细胞与再生医学发展前瞻

科技导报 2016年第20期34卷 25-33页

作者：刘晶曹尚涛蔡景蕾裴端卿中国科学院广州生物医药与健康研究院中国科学院再生生物学重点实验室广东省干细胞与再生医学重点实验室华南干细胞与再生医学研究所广州510530

干细胞与再生医学是当今生命科学领域研究的前沿与热点。近年来,涌现出一系列突破性的研究成果,将干细胞研究推向新的历史阶段,为再生医学的发展带来深刻变革。本文分析体内外不同来源的干细胞在再生医学的治疗应用、小分子化合物在细... 详细信息

干细胞与再生医学是当今生命科学领域研究的前沿与热点。近年来,涌现出一系列突破性的研究成果,将干细胞研究推向新的历史阶段,为再生医学的发展带来深刻变革。本文分析体内外不同来源的干细胞在再生医学的治疗应用、小分子化合物在细胞命运转变机理研究和功能性细胞获取方面的研究现状与前景,讨论了干细胞与再生医学在临床治疗应用的安全性与规范性。

关键词：干细胞再生医学细胞命运转变临床治疗

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新工科背景下创新创业教育的实践探究

创新创业理论研究与实践 2019年第4期2卷 147-148页

作者：刘晓宁蔡景蕾哈尔滨工业大学(威海)信息科学与工程学院

在新工科背景下,创新创造教育得到了高度关注,但是在实际教学中,部分学校因为师资力量、教学模式、考评机制等方面未能满足创新创造教育需求,导致教学效果不理想。该文首先概述了新工科背景下实施创新创业教育的重要价值,随后基于现阶... 详细信息

在新工科背景下,创新创造教育得到了高度关注,但是在实际教学中,部分学校因为师资力量、教学模式、考评机制等方面未能满足创新创造教育需求,导致教学效果不理想。该文首先概述了新工科背景下实施创新创业教育的重要价值,随后基于现阶段教学中存在的突出问题,分别从完善教育体系、增强师资力量、搭建创新创业平台等方面,提出了针对性的优化建议。

关键词：新工科创新创业考评机制创业孵化基地

Single cell atlas of developing mouse dental germs reveals populations of CD24^(+)and Plac8^(+)odontogenic cells

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Science Bulletin 2022年第11期67卷 1154-1169页

作者： Yaofeng Wang Yifan Zhao Shubin Chen Xiaoming Chen Yanmei Zhang Hong Chen Yuansong Liao Jiashu Zhang Di Wu Hongxing Chu Hongying Huang caixia Wu Shijuan Huang Huichao Xu Bei Jia Jie Liu Bo Feng Zhonghan Li Dajiang Qin Duanqing Pei jinglei cai Innovation Centre for Advanced Interdisciplinary Medicine The Fifth Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510799China CAS Key Laboratory of Regenerative Biology GIBH-CUHK Joint Research Laboratory on Stem Cell and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China Centre for Regenerative Medicine and Health Hong Kong Institute of Science&InnovationChinese Academy of SciencesHong KongChina Bioland Laboratory Guangzhou Regenerative Medicine and Health Guangdong LaboratoryGuangzhou 510005China Guangdong Provincial People’s Hospital Ganzhou Hospital Ganzhou Municipal HospitalGanzhou 341099China State Key Laboratory of Oral Disease West China Hospital of StomatologyCenter of Growth Metabolism and AgingKey Laboratory of Bio-Resource and Eco-Environment of Ministry of EducationCollege of Life SciencesSichuan UniversityChengdu 610041China Department of Regeneration Medicine School of Pharmaceutical ScienceJilin UniversityChangchun 130012China Department of Periodontics and Implantology Stomatological HospitalSouthern Medical University(Guangdong Provincial Stomatological Hospital)Guangzhou 510515China Animal Center Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China The Center for Prenatal and Hereditary Disease Diagnosis Department of Obstetrics and GynecologyNanfang HospitalSouthern Medical UniversityGuangzhou 510515China Department of Obstetrics and Gynecology Nanfang HospitalSouthern Medical UniversityGuangzhou 510515China School of Biomedical Sciences The Chinese University of Hong KongHong Kong SARChina Laboratory of Cell Fate Control School of Life SciencesWestlake UniversityHangzhou 310024China

The spatiotemporal relationships in high-resolution during odontogenesis remain poorly *** report a cell lineage and atlas of developing mouse *** performed a large-scale(92,688 cells)single cell RNA sequencing,tracing the cell trajectories during odontogenesis from embryonic days 10.5 to *** with an assay for transposase-accessible chromatin with high-throughput sequencing,our results suggest that mesenchymal cells show the specific transcriptome profiles to distinguish the tooth ***,we identified key gene regulatory networks in teeth and bone formation and uncovered spatiotemporal patterns of odontogenic mesenchymal ***24^(+)and Plac8^(+)cells from the mesenchyme at the bell stage were distributed in the upper half and preodontoblast layer of the dental papilla,respectively,which could individually induce nonodontogenic epithelia to form tooth-like ***,the Plac8^(+)tissue we discovered is the smallest piece with the most homogenous cells that could induce tooth regeneration to *** work reveals previously unknown heterogeneity and spatiotemporal patterns of tooth germs that may lead to tooth regeneration for regenerative dentistry.

关键词： Single cell RNA-seq Dental germ development Spatiotemporal pattern Odontogenesis CD24^(+)odontogenic cells Plac8^(+)odontogenic cells