检索结果-南通市图书馆

Screening for a new Streptomyces strain capable of efficient keratin degradation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2007年第9期19卷 1125-1128页

作者： CHAO Ya-peng XIE Fu-hong YANG jing LU jing-hua qian Shi-jun State Key Laboratories of Transducer Technology Institute of Microbiology Chinese Academy of Sciences Beijing 100080 China.

Keratinous wastes could be degraded by some microorganisms in nature. Native human foot skin （NHFS） was used as sole nitrogen source to screen microorganisms with keratin-degrading capability. From approximately 200 strains, a strain of Streptomyces sp. strain No. 16 was found to possess the strongest keratinolytic activity, and the total activity in the culture was 110 KU/ml with specific activity of 2870 KU/mg protein （KU： keratinase unit）. Substrate specificity test indicated that the crude keratinase could degrade keratin azure, human hair, cock feathers and collagen. The optimal pH of the crude keratinase ranged from 7.5 to 10 and the temperature ranged from 40℃ to 55℃. Metal chelating agent ethylenediamine tetraacetic acid obviously stimulated the keratinolytic activity but suppressed the proteolytic activity. To our knowledge, this is the first report on specific induction of keratinases by NHFS from an actinomycete. Moreover, excellent characteristics of its crude keratinase may lead to the potential application in waste treatment and recovery, poultry and leather industry, medicine, and cosmetic development.

关键词： keratinolytic capability Streptomyces sp. native human foot skin (NHFS) keratinase substrate specificity

Correlation analysis of national elite Chinese male table tennis players’ shoulder proprioception and muscle strength

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2022年第24期10卷 8514-8524页

作者： Xue-Dong Shang En-Ming Zhang Zhen-Lei Chen Lei Zhang jing-hua qian Institute of Sport Medicine National Research Institute of Sports MedicineBeijing 100061China School of Sports Medicine and Rehabilitation Beijing Sport UniversityBeijing 100084China School of Physical Education Hubei University of EducationWuhan 430205Hubei ProvinceChina Graduate School Shandong Sport UniversityJinan 250102Shandong ProvinceChina

BACKGROUND Shoulder is the most injured part in table tennis players,and it takes multiple roles in transmitting power and striking the center of the ball during the *** is strongly correlated with high level of athletic *** is customary to assume that there is a correlation between proprioception and muscle strength and therefore proprioceptive assessment and rehabilitation is often *** To investigate the correlation between isokinetic muscle strength and proprioception in the internal and external rotation muscle groups of elite Chinese male table tennis players,to provide reference for physical training and rehabilitation of elite table tennis *** A total of 19 national elite table tennis players from the Chinese National Table Tennis Team were recruited in this *** of them had more than 10 years training experience and had participated major competitions such as the National Games and World Youth *** 2000 was used to test the peak torque of internal and external rotation isokinetic concentric contraction of the athletes'bilateral shoulder joints at low speed(60°/s)and high speed(180°/s)respectively;IsoMed 2000 was used to conduct the Joint Position Reproduction test to evaluate the athletes'proprioceptive ability capacity at low speed(60°/s)and high speed(180°/s)*** the data satisfied the normal distribution,the correlation between the differences in peak torque s and angles in different directions was analyzed using a Pearson simple linear model;otherwise,Spearman correlation analysis was *** comparison of proprioceptive ability between the table tennis racket-holding hand and non-racket-holding hands was performed using independent samples t-test if the data satisfied a normal distribution;otherwise,the Mann-Whitney U test was *** There was no direct linear correlation between the strength and proprioceptive correlation analysis at slow speed(60°/s)and fast s

关键词： Elite table tennis player Shoulder Proprioception Muscle strength Correlation analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

第1章制冷行业政策分析

制冷技术 2018年第A1期38卷 2-5页

作者：荆华乾中国制冷学会北京100142

2017年是我国冷链物流行业快速发展的一年,国家对冷链物流行业也是高度重视。同时推动了制冷行业的发展,国家先后推出很多扶持性政策。整个行业标准化、平台化、透明化也逐步凸显,行业资源整合与市场竞争更加激烈,生鲜物流体系持续扩张... 详细信息

2017年是我国冷链物流行业快速发展的一年,国家对冷链物流行业也是高度重视。同时推动了制冷行业的发展,国家先后推出很多扶持性政策。整个行业标准化、平台化、透明化也逐步凸显,行业资源整合与市场竞争更加激烈,生鲜物流体系持续扩张,生鲜电商也快速发展。

关键词：政策扶持冷链物流规划

在线全文

学校读者我要写书评

暂无评论

制冷行业政策分析

制冷技术 2016年第A01期36卷 3-7页

作者：荆华乾中国制冷学会北京100142

我国是传统的农业大国，也是易腐食品的生产、加工、消费和出口大国。整体制冷行业面向社会仍有较大发展空间和各方面的突破潜力。我国政府、各地政府和相关部门仍会在各环节对制冷行业进行大力支持并起到积极的促进作用。

关键词：政策扶持冷链物流规划

在线全文

学校读者我要写书评

暂无评论

制冷行业政策分析

制冷技术 2017年第S1期37卷 3-6页

作者：荆华乾中国制冷学会北京100142

“十三五”开局之年，我国政府进一步规划建议将食品安全问题提到国家战略高度，提出实施食品安全战略。作为保证产品品质和质量安全、减少损耗、防止污染的特殊供应链系统，“冷链”对肉、禽、水产、蔬菜、水果、蛋等生鲜农产品的安全... 详细信息

“十三五”开局之年，我国政府进一步规划建议将食品安全问题提到国家战略高度，提出实施食品安全战略。作为保证产品品质和质量安全、减少损耗、防止污染的特殊供应链系统，“冷链”对肉、禽、水产、蔬菜、水果、蛋等生鲜农产品的安全起到了保驾护航的作用。农产品冷链物流发展环境逐步完善。

关键词：政策扶持冷链物流规划

非酒精性脂肪性肝病患者血清claudin-1、claudin-4的变化及其与病情程度的相关性

在线全文

学校读者我要写书评

暂无评论

世界华人消化杂志 2017年第19期25卷 1772-1776页

作者：周菁钱玲华胡碧群杭州市余杭区第二人民医院浙江省杭州市311121

目的观察非酒精性脂肪性肝病患者血清闭锁蛋白1(claudin-1)和闭锁蛋白4(claudin-4)的变化,分析其与肠道黏膜屏障功能及病情程度的关系.方法选取79例非酒精性脂肪性肝病患者,按病情程度分为轻度(n=23)、中度(n=27)、重度(n=29),采用ELIS... 详细信息

目的观察非酒精性脂肪性肝病患者血清闭锁蛋白1(claudin-1)和闭锁蛋白4(claudin-4)的变化,分析其与肠道黏膜屏障功能及病情程度的关系.方法选取79例非酒精性脂肪性肝病患者,按病情程度分为轻度(n=23)、中度(n=27)、重度(n=29),采用ELISA法测定其血清二胺氧化酶(diamine oxidase,DAO)、内毒素(endotoxin,ET)及血清claudin-1及claudin-4含量,并选取同期健康体检者40例作为对照.结果与对照组比较,患者组血清DAO、ET及claudin-4含量均明显升高(P<0.01,P<0.05),而血清claudin-1含量则显著上升(P<0.01).患者组血清DAO、ET及claudin-4含量随着病情程度的加重呈上升趋势,而血清claudin-1含量出现下降趋势.重度患者血清DAO、ET及claudin-4含量较轻中度患者明显上升(P<0.01,P<0.05);血清claudin-1含量则较轻中度患者明显下降(P<0.01,P<0.05).相关性分析表明,血清claudin-1含量与血清DAO、ET含量、病情程度呈明显负相关(r=-0.695、-0.738、-0.741,P<0.01);claudin-4含量与血清DAO、ET含量、病情程度呈明显正相关(r=0.779、0.755、0.875,P<0.01).结论非酒精性脂肪性肝病患者存在明显的肠黏膜功能障碍,且与其病情程度密切相关;肠黏膜紧密连接蛋白claudin-1、claudin-4表达异常可能是发生肠黏膜功能障碍的重要机制.

关键词：非酒精性脂肪性肝病肠黏膜屏障功能闭锁蛋白1 闭锁蛋白4

Small extracellular vesicles derived from human induced pluripotent stem cell-differentiated neural progenitor cells mitigate retinal ganglion cell degeneration in a mouse model of optic nerve injury

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第2期20卷 587-597页

作者： Tong Li Hui-Min Xing Hai-Dong qian Qiao Gao Sheng-Lan Xu hua Ma Zai-Long Chi State Key Laboratory of Ophthalmology Optometry and Visual ScienceEye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina National Clinical Research Center for Ocular Diseases Eye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina

Several studies have found that transplantation of neural progenitor cells(NPCs)promotes the survival of injured ***,a poor integration rate and high risk of tumorigenicity after cell transplantation limits their clinical *** extracellular vesicles(sEVs)contain bioactive molecules for neuronal protection and *** studies have shown that stem/progenitor cell-derived sEVs can promote neuronal survival and recovery of neurological function in neurodegenerative eye diseases and other eye *** this study,we intravitreally transplanted sEVs derived from human induced pluripotent stem cells(hiPSCs)and hiPSCs-differentiated NPCs(hiPSC-NPC)in a mouse model of optic nerve *** results show that these intravitreally injected sEVs were ingested by retinal cells,especially those localized in the ganglion cell *** with hiPSC-NPC-derived sEVs mitigated optic nerve crush-induced retinal ganglion cell degeneration,and regulated the retinal microenvironment by inhibiting excessive activation of *** analysis further revealed that hiPSC-NPC derived sEVs transported neuroprotective and anti-inflammatory miRNA cargos to target cells,which had protective effects on RGCs after optic nerve *** findings suggest that sEVs derived from hiPSC-NPC are a promising cell-free therapeutic strategy for optic neuropathy.

关键词： exosome miRNA neural progenitor cell neurodegeneration neuroinflammation neuroprotection optic nerve crush optic neuropathy retinal ganglion cell small extracellular vesicles

在线全文

学校读者我要写书评

暂无评论

番茄冷链运输保鲜技术研究

制冷技术 2014年第5期34卷 49-53页

作者：高恩元荆华乾中国制冷学会北京100142

在冷链运输过程中,控制冷链运输的时长和温度能有效的减少运输过程中的损失和能耗,使得果蔬快速、优质地运输到目的地。模拟了三种冷链运输方式:EPS保温箱运输、蓄冷保温箱运输和5℃冷链运输;通过对番茄品温、营养物质和感官评价来确定... 详细信息

在冷链运输过程中,控制冷链运输的时长和温度能有效的减少运输过程中的损失和能耗,使得果蔬快速、优质地运输到目的地。模拟了三种冷链运输方式:EPS保温箱运输、蓄冷保温箱运输和5℃冷链运输;通过对番茄品温、营养物质和感官评价来确定不同条件下的不同运输方式。结果表明,EPS保温箱适合短距离运输,运输时长不超过24 h;蓄冷保温箱适合中短距离运输,时长不超过48 h;5℃冷链运输适合长距离运输,番茄在72 h后仍能保持良好的品质。

关键词：冷链物流番茄营养品质感官评价

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong jing-jing Hu Yan Yu Xiao-Li Li Xiao-Ting Sun Li-Ting Wang Xia Wu Ling-Jie Zhu Xiao-Jia Yang qian-Ru He Fei Ding Hai-Yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors