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The NAC transcription factor LuNAC61 negatively regulates fiber development in flax (Linum usitatissimum L.)

Journal of Integrative Agriculture 2024年第3期23卷 795-805页

作者： Dongwei Xie jing Li Wan Li Lijun Sun zhigang Dai Wenzhi zhou Jianguang Su Jian Sun School of Life Sciences Nantong UniversityNantong 226019China Institute of Crop Cultivation and Tillage Heilongjiang Academy of Agricultural SciencesHarbin 150086China Institute of Bast Fiber Crops Chinese Academy of Agricultural SciencesChangsha 410221China Jiangsu Sanshu Biotechnology Co.Ltd. Nantong 226001China

Flax is a crucial fiber crop that exhibits excellent textile properties and serves as a model plant for investigating phloem fiber development. The regulation of multiple genes significantly influences fiber development, notably involving NAC(NAM, ATAF1/2, CUC2) transcription factors in forming the fiber secondary cell wall(SCW).overexpression of LuNAC61 in flax resulted in sparse top meristematic zone leaves and significantly reduced stem cellulose content. Scanning electron microscopy and staining observations revealed a significant reduction in fiber bundles. β-Glucuronidase(GUS) staining analysis demonstrated high activity of the LuNAC61 promoter in the bast fibers of the flax stem. Additionally, several members of the LuPLATZ and LuCesA families exhibited significant coexpression with LuNAC61. Subcellular localization indicated the presence of LuPLATZ24 protein in the nucleus and cytoplasm, LuNAC61 protein exclusively in the nucleus, and LuCesA10 in the nucleus and endoplasmic reticulum. LuPLATZ24 positively regulates LuNAC61, whereas LuNAC61 negatively affects LuCesA10, suggesting the involvement of a metabolic network in regulating flax fiber development. In conclusion, this study provides a critical opportunity for a comprehensive and in-depth analysis of the mechanisms governing flax fiber development and the potential use of biotechnology to enhance flax fiber yield.

关键词： flax fiber development Lu NAC61 gene function gene interaction

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Semaphorin 7A impairs barrier function in cultured human corneal epithelial cells in a manner dependent on nuclear factor-kappa B

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International Journal of ophthalmology(English edition) 2024年第3期17卷 444-453页

作者： Cheng-Cheng Yang Xiu-Xia Yang Xiao-jing zh.o Heng Wang Zi-Han Guo Kai Jin Yang Liu Bin-Hui Li Department of ophthalmology the Fifth Affiliated HospitalSun Yat-sen UniversityZhuhai 519000Guangdong ProvinceChina Department of ophthalmology Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical UniversityHangzhou 310006Zhejiang ProvinceChina Eye Institute of Xiamen University Fujian Provincial Key Laboratory of Ophthalmology and Visual ScienceSchool of MedicineXiamen UniversityXiamen 361005Fujian ProvinceChina

●AIM:To evaluate the role of semaphorin 7A(Sema7A)and its associated regulatory mechanisms in modulating the barrier function of cultured human corneal epithelial cells(HCEs).●METHoDS:Barrier models of HCEs were treated with recombinant human Sema7A at concentrations of 0,125,250,or 500 ng/mL for 24,48,or 72h in *** electrical resistance(TEER)as well as Dextran-fluorescein isothiocyanate(FITC)permeability assays were conducted to assess barrier *** quantify tight junctions(TJs)such as occludin and zonula occludens-1(Zo-1)at the mRNA level,reverse transcriptionpolymerase chain reaction(RT-PCR)analysis was *** was used to examine the activity of the nuclear factor-kappa B(NF-κB)signaling pathway and the production of TJs *** analyses were employed to localize the ***-linked immunosorbent assay(ELISA)and RT-PCR were utilized to observe changes in interleukin(IL)-1β*** investigate the role of NF-κB signaling activation and IL^(-1)βin Sema7A’s anti-barrier mechanism,we employed 0.1μmol/L IκB kinase 2(IKK2)inhibitor IV or 500 ng/mL IL^(-1)receptor(IL-1R)antagonist.●RESULTS:Treatment with Sema7A resulted in decreased TEER and increased permeability of Dextran-FITC in HCEs through down-regulating mRNA and protein levels of TJs in a time-and dose-dependent manner,as well as altering the localization of ***,Sema7A stimulated the activation of inhibitor of kappa B alpha(IκBα)and expression of IL-1β.The anti-barrier function of Sema7A was significantly suppressed by treatment with IKK2 inhibitor IV or IL-1R antagonists.●CoNCLUSIoN:Sema7A disrupts barrier function through its influence on NF-κB-mediated expression of TJ proteins,as well as the expression of IL-1β.These findings suggest that Sema7A could be a potential therapeutic target for the diseases in corneal epithelium.

关键词： human corneal epithelial barrier function transepithelial electrical resistance zonula occludens-1 occludin nuclear factor-kappa B

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Dip2a regulates stress susceptibility in the basolateral amygdala

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Neural Regeneration Research 2025年第6期20卷 1735-1748页

作者： jing Li Zixuan He Weitai Chai Meng Tian Huali Yu Xiaoxiao He Xiaojuan zhu Key Laboratory of Molecular Epigenetics Ministry of Education Institute of Genetics and CytologyNortheast Normal UniversityChangchunJilin ProvinceChina

Dysregulation of neurotransmitter metabolism in the central nervous system contributes to mood disorders such as depression, anxiety, and post–traumatic stress disorder. Monoamines and amino acids are important types of neurotransmitters. our previous results have shown that disco-interacting protein 2 homolog A(Dip2a) knockout mice exhibit brain development disorders and abnormal amino acid metabolism in serum. This suggests that DIP2A is involved in the metabolism of amino acid–associated neurotransmitters. Therefore, we performed targeted neurotransmitter metabolomics analysis and found that Dip2a deficiency caused abnormal metabolism of tryptophan and thyroxine in the basolateral amygdala and medial prefrontal cortex. In addition, acute restraint stress induced a decrease in 5-hydroxytryptamine in the basolateral amygdala. Additionally, Dip2a was abundantly expressed in excitatory neurons of the basolateral amygdala, and deletion of Dip2a in these neurons resulted in hopelessness-like behavior in the tail suspension test. Altogether, these findings demonstrate that DIP2A in the basolateral amygdala may be involved in the regulation of stress susceptibility. This provides critical evidence implicating a role of DIP2A in affective disorders.

关键词： 5-hydroxytryptamine acute restraint stress basolateral amygdala CaMKII neurons DIP2A metabolomics neurotransmitters principal component analysis stress susceptibility tryptophan

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早期康复护理对吉兰-巴雷综合征患者肌力及日常生活活动能力的影响

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罕少疾病杂志 2024年第8期31卷 148-149,158页

作者：位亚娟王静张新勋郑州大学第一附属医院神经重症监护病房河南郑州450052

目的探究早期康复护理在吉兰-巴雷综合征(GBS)患者中的应用效果。方法选取2021年1月~2023年12月本院收治的GBS患者72例,根据护理方案进行分组,对照组40例患者进行常规护理,观察组32例患者进行早期康复护理。比较两组的四肢肌力、日常生... 详细信息

目的探究早期康复护理在吉兰-巴雷综合征(GBS)患者中的应用效果。方法选取2021年1月~2023年12月本院收治的GBS患者72例,根据护理方案进行分组,对照组40例患者进行常规护理,观察组32例患者进行早期康复护理。比较两组的四肢肌力、日常生活活动能力、自我效能感以及生活质量。结果干预前两组患者各项指标之间均无显著性差异(P>0.05);干预后,观察组上、下肢近端及远端肌力、改良Barthel指数(MBI)、一般自我效能感量表(GSES)、简明健康状况问卷(SF-36)评分较对照组均明显升高(P<0.05)。结论在GBS患者中,应用早期康复护理可明显改善其四肢肌力,显著提升患者的日常生活活动能力,有效提高患者的自我效能感及生活质量,值得临床推广。

关键词：早期康复护理 GBS 肌力日常生活活动能力

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重庆市农业科技创新能力评价研究

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生产力研究 2024年第6期 43-48,F0003页

作者：曾琼万立志尹朝静马强重庆生产力促进中心(重庆市科学技术信息中心) 重庆401147 西南大学经济管理学院重庆400715 重庆市农业科学院重庆401329

根据农业科技创新内涵以及农业科技创新发展目标要求,从农业科技创新环境、投入、产出和贡献四个方面构建重庆市农业科技创新能力评价指标体系,并对2022年重庆市37个行政区县(不含渝中区)的农业科技创新能力进行评价和分析。研究发现重... 详细信息

根据农业科技创新内涵以及农业科技创新发展目标要求,从农业科技创新环境、投入、产出和贡献四个方面构建重庆市农业科技创新能力评价指标体系,并对2022年重庆市37个行政区县(不含渝中区)的农业科技创新能力进行评价和分析。研究发现重庆市农业科技创新的主要特征和短板,并从强化科研创新供给、提高农业科技成果量、发展数字农业农村、提高农业科技智慧度等5个方面提出提升重庆市农业科技创新能力的建议。

关键词：数实融合理论机理路径选择重庆市

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论冯道刻印九经的印刷史意义

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印刷文化（中英文） 2024年第3期 66-75,151页

作者：陈静济南大学文学院

五代时期的冯道通过主持刻印儒家典籍九经,在历史上首次将雕版印刷术成功地引入主流书籍出版领域。冯道刻印九经采取了四种影响深远的举措。第一,将校书确立为印刷复制前的重要环节;第二,书籍装帧由卷轴变为册页;第三,确立了雕版书籍的... 详细信息

五代时期的冯道通过主持刻印儒家典籍九经,在历史上首次将雕版印刷术成功地引入主流书籍出版领域。冯道刻印九经采取了四种影响深远的举措。第一,将校书确立为印刷复制前的重要环节;第二,书籍装帧由卷轴变为册页;第三,确立了雕版书籍的版式规范;第四,九经刻版印卖,批量生产,开官府售书之先河。这四种举措将雕版印刷技术与书籍出版成功结合,冯道印行九经也因此成为书籍由抄本出版转为雕版出版的转折点。校书、复制、售书三者合一,这是雕版出版已臻成熟的历史性标志,书籍出版领域从此全面引入雕版印刷术,中国书籍出版进入了印本时代。

关键词：冯道雕版印刷书籍出版九经

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Accurate prediction of myopic progression and high myopia by machine learning

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Precision Clinical Medicine 2024年第1期7卷 14-20页

作者： Jiahui Li Simiao Zeng zhihuan Li Jie Xu zh.o Sun jing zh.o Meiyan Li Zixing Zou Taihua Guan Jin Zeng zhuang Liu Wenchao Xiao Ran Wei Hanpei Miao Ian Ziyar Junxiong Huang Yuanxu Gao Yangfa Zeng Xing-Tao zhou Kang zhang Guangzhou Women and Children's Medical Center Guangzhou Medical UniversityGuangzhou 510120China Guangzhou National Laboratory Guangzhou 510o0oChina zhuhai International Eye Center of the First Affiliated Hospital of Faculty of Medicine Macao University of Science and TechnologyZhuhai 519000China Beijing Institute of ophthalmology Beijing Tongren Eye CenterBeijing Tongren HospitalCapital Medical UniversityBeijing 100069China Key Laboratory of Myopia Ministry of HealthShanghai Key Laboratory of Visual Impairment and RestorationDepartment of OphthalmologyEye InstituteEye and EarNoseand Throat HospitalFudan UniversityShanghai 200031China DongguanPeople's Hospital The First School of Clinical MedicinelSouthern Medical UniversityDongguan 523059China State Key Laboratory of ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060China Wenzhou Eye Hospital and Institute for Advanced Study on Eye Health and Diseases Wenzhou Medical UniversityWenzhou 32500oChina

Background Myopia is a leading cause of visual impairment in Asia and ***,accurately predicting the progression of myopia and the high risk of myopia remains a *** study aims to develop a predictive model for the development of *** We first retrospectively gathered 612530 medical records from five independent cohorts,encompassing 227543 patients ranging from infants to young ***,we developed a multivariate linear regression algorithm model to predict the progression of myopia and the risk of high *** The model to predict the progression of myopia achieved an R^(2) value of 0.964 vs a mean absolute error(MAE)of 0.119D[95%confidence interval(CI):0.119,1.146]in the internal validation *** demonstrated strong generalizability,maintaining consistent performance across external validation sets:R^(2)=0.950 vs MAE=0.119D(95%CI:0.119,1.136)in validation study 1,R^(2)=0.950 vs MAE=0.121D(95%CI:0.121,1.144)in validation study 2,and R^(2)=0.806 vs MAE=−0.066D(95%CI:−0.066,0.569)in the Shanghai Children Myopia *** the Beijing Children Eye Study,the model achieved an R^(2) of 0.749 vs a MAE of 0.178D(95%CI:0.178,1.557).The model to predict the risk of high myopia achieved an area under the curve(AUC)of 0.99 in the internal validation set and consistently high area under the curve values of 0.99,0.99,0.96 and 0.99 in the respective external validation *** our study demonstrates accurate prediction of myopia progression and risk of high myopia providing valuable insights for tailoring strategies to personalize and optimize the clinical management of myopia in children.

关键词： myopia progression machine learning prevention precision medicine

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Behavior, distribution and environmental influence of arsenic in a typical lead smelter

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Journal of Central South University 2015年第4期22卷 1276-1286页

作者：柴立元史美清梁彦杰汤景文李青竹 School of Metallurgy and Environment Central South University Chinese National Engineering Research Center for Control and Treatment of Heavy Metal Pollution (Central South University)

A field study was conducted to determine the behavior and distribution of arsenic during the pyrometallurgy process in a typical SKS(Shuikoushan) lead smelter in Hunan province, China. Environmental influences of arsenic in selected samples were evaluated. Arsenic contents in all input and output samples vary from 0.11% in raw lead to 6.66% in collected dust-2. More arsenic is volatilized in blast furnace and fuming furnace(73.02% of arsenic input) than bottom blowing furnace(10.29% of arsenic input).There are 78.97%, 13.69%, 7.31% of total arsenic distributed in intermediate materials, stockpiled materials and unorganized emissions, respectively. Matte slag-2, collected dust-1 and secondary zinc oxide are hazardous based on the arsenic concentrations of toxicity characteristic leaching procedure. According to risk assessment code(RAC) guideline, arsenic in collected dust-1 poses a very serious risk to the surrounding environment, arsenic in speiss, matte slag-2, water-quenched slag and secondary zinc oxide show low risk, while arsenic in matte slag-1, collected dust-2 and post dust has no risk to the environment.

关键词： arsenic behavior distribution environmental influence SKS lead smelter

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First principles study of behavior of helium at Fe(110)-graphene interface

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Chinese Physics B 2021年第4期30卷 432-437页

作者： Yan-Mei jing Shao-Song Huang Key Laboratory of Material Modification by Laser Ion and Electron Beams(Dalian University of Technology)Ministry of EducationDalian 116024China

Recently,metal-graphene nanocomposite system has aroused much interest due to its radiation tolerance ***,the related atomic mechanism for the metal-graphene interface is still ***,stainless steels with Fe as main matrix are widely used in nuclear ***,in this study,the atomic behaviors of point defects and helium(He) atoms at the Fe(110)-graphene interface are investigated systematically by first principles *** results indicate that graphene interacts strongly with the Fe(110) *** comparison with those of the original graphene and bulk Fe,the formation energy values of C vacancies and Fe point defects decrease significantly for Fe(110)-***,as He atoms have a high migration barrier and large binding energy at the interface,they are trapped at the interface once they enter into *** theoretical results suggest that the Fe(110)-graphene interface acts as a strong sink that traps defects,suggesting the potential usage of steel-graphene with multiply interface structures for tolerating the radiation damage.

关键词： Fe(110)-graphene helium interface first principles calculations

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固定偏角垂直轴潮流水轮机叶片安装角研究

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哈尔滨工程大学学报 2011年第7期32卷 853-857页

作者：李志川孙科荆丰梅张亮哈尔滨工程大学海洋可再生能源研究所黑龙江哈尔滨150001

叶片安装角是影响固定偏角垂直轴水轮机性能的关键参数之一.为了提高水轮机性能,应用模型试验和数值模拟的方法,研究了安装角对水轮机叶片载荷和叶轮整体性能的影响.通过能量利用率的数值计算结果与试验结果对比,验证了数值模拟结果的... 详细信息

叶片安装角是影响固定偏角垂直轴水轮机性能的关键参数之一.为了提高水轮机性能,应用模型试验和数值模拟的方法,研究了安装角对水轮机叶片载荷和叶轮整体性能的影响.通过能量利用率的数值计算结果与试验结果对比,验证了数值模拟结果的可靠性.研究结果表明,叶片安装时首部外偏一定角度,可以提高叶轮的能量利用率,并且可以降低叶片载荷,这有利于水轮机的长期运转;叶片安装时首部内偏一定角度,对叶轮的能量利用率和叶片载荷均不利.

关键词：潮流能垂直轴水轮机叶片安装角能量利用率叶片载荷

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