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Molecular cloning, characterization and expression analysis of a catalase gene in Paphia textile

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Acta Oceanologica Sinica 2016年第8期35卷 65-73页

作者： WU Xiangwei LI Jiakai tan jing LIU Xiande Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment Fisheries College Jimei University Animal Science and Technology College Yunnan Agricultural University

Catalase is an important antioxidant protein that can protect organisms against various forms of oxidative damage by eliminating hydrogen peroxide. In this study, the catalase c DNA of Paphia textile（Pt CAT） was cloned using RTPCR and rapid amplification of c DNA ends（RACE）. Pt CAT is 1 921 bp long and consists of a 5′-UTR of 50 bp, a 3′-UTR of 349 bp, and an ORF of 1 542 bp that encodes 513 amino acids with a molecular weight of 58.4 k D and an estimated isoelectric point of 8.2. Sequence alignment indicated that Pt CAT contained a highly conserved catalytic signature motif（^（61）FNRERIPERVVHAKGAG^（77））, a proximal heme-ligand signature sequence（^（352）RLFSYSDP^（359））, and three catalytic amino acid residues（H^（72）, N^（145）, and Y^（356））. Pt CAT also contains two putative N-glycosylation sites（^（34）NKT^（36） and ^（437）NFT^（439）） and a peroxisome-targeting signal（^（511）AQL^（513））. Furthermore, Pt CAT shares 53%–88% identity and 29%–89% similarity with other catalase amino acid sequences. Pt CAT m RNA was present in all tested organs, including the heart, digestive gland, adductor muscle, gonad, gill, and mantle, but its expression was highest in the digestive gland. High-temperature-induced stress produced two expression patterns of Pt CAT m RNA： first, an initial up-regulation followed by a down-regulation in the heart, digestive gland, and gonad and, second, consistent down-regulation in all other organs. These results demonstrate that Pt CAT is a typical member of the catalase family and might be involved in the responses to harmful environmental factors.

关键词： Paphia textile catalase(CAT) cloning sequence analysis expression analysis high temperature stress

Visible-Light-Mediated Photocatalyst-Free Hydroacylation of Azodicarboxylic Acid Derivatives with 4-Acyl-1,4-dihydropyridines

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Chinese Journal of Chemistry 2024年第11期42卷 1230-1236页

作者： Li Liu jing Wang Xiaoying Feng Kun Xu Wei Liu Xia Peng Hongguang Du Jiajing tan Department of Organic Chemistry College of ChemistryBeijing University of Chemical TechnologyBeijing100029 China Sinopec Beijing Research Institute of Chemical Industry Beijing100013 China Faculty of Environment and Life Beijing University of TechnologyBeijing100124 China

A visible-light-enabled,photocatalyst-free hydroacylation reaction of azodicarboxylic acid derivatives was *** radical conjugate addition(RCA)protocol relied on the dual role of 4-acyl-1,4-dihydropyridine(acyl-DHP)reagents that besides being as radical reservoirs,they also enabled the conversion of radical adducts to anion intermediates via ***“catalyst-oxidant-additive free”conditions,a wide range of structurally different acyl hydrazide products were readily obtained in 56%—99%*** utility of this transformation was further demonstrated by the scale-up synthesis and downstream derivatization.

关键词： Azo compounds Radicals Acylation 4-Acyl-1,4-dihydropyridine Photochemistry

地中海饮食干预联合有氧运动训练对2型糖尿病患者脂糖代谢水平、肠道菌群及生活质量的影响

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现代生物医学进展 2024年第1期24卷 79-82,30页

作者：杜乃立朱晓巍万霞姚孝娟谈静曹翠萍南京医科大学附属无锡人民医院内分泌科江苏无锡214000 南京医科大学附属无锡人民医院老年医学科江苏无锡214000 南京医科大学附属无锡人民医院急诊科江苏无锡214000

目的:探讨地中海饮食干预联合有氧运动训练对2型糖尿病(T2DM)患者脂糖代谢水平、肠道菌群及生活质量的影响。方法:选择2021年6月~2022年12月我院110例T2DM患者为研究对象,随机数字表法随机分组,各55例。对照组给予常规健康教育及饮食、... 详细信息

目的:探讨地中海饮食干预联合有氧运动训练对2型糖尿病(T2DM)患者脂糖代谢水平、肠道菌群及生活质量的影响。方法:选择2021年6月~2022年12月我院110例T2DM患者为研究对象,随机数字表法随机分组,各55例。对照组给予常规健康教育及饮食、运动干预,观察组给予地中海饮食干预及有氧运动训练。比较两组干预前后脂糖代谢指标[空腹血糖(FPG)、餐后2 h血糖(2hPG)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)]、肠道菌群、生活质量变化及并发症发生率。结果:干预后观察组HDL-C水平高于对照组,FPG、2hPG、TC、TG、LDL-C水平低于对照组(P0.05)。结论:地中海饮食干预联合有氧运动训练能有效纠正T2DM患者肠道菌群紊乱状态,改善脂糖代谢,减少并发症发生,提高生活质量。

关键词：地中海饮食有氧运动 2型糖尿病脂糖代谢肠道菌群生活质量

Valence-tailored copper-based nanoparticles for enhanced chemodynamic therapy through prolonged ROS generation and potentiated GSH depletion

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Nano Research 2024年第7期17卷 6342-6352页

作者： Xinyang Li Binbin Ding jing Li Di Han Hao Chen Jia tan Qi Meng Pan Zheng Ping’an Ma Jun Lin State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei 230026China

Chemodynamic therapy(CDT),an inventive approach to cancer treatment,exploits innate chemical processes to trigger cell death through the generation of reactive oxygen species(ROS).While offering advantages over conventional treatments,the optimization of CDT efficacy presents challenges stemming from suboptimal catalytic efficiency and the counteractive ROS scavenging effect of intracellular glutathione(GSH).In this study,we aim to address this dual challenge by delving into the role of copper valence states in *** the unique attributes of copper-based nanoparticles,especially zero-valent copper nanoparticles(CuPd NPs),we aim to enhance the therapeutic potential of *** experiments reveal that zero-valent CuPd NPs outperform divalent copper nanoparticles(Ox-CuPd NPs)by displaying superior catalytic performance and sustaining ROS generation through a dual approach integrating peroxidase-like(POD-like)activity and Cu+***,zero-valent NPs exhibit enhanced GSH depletion compared to their divalent counterparts,thereby intensifying CDT and inducing ferroptosis,ultimately resulting in high-efficiency tumor growth *** findings reveal the impact of valences on CDT,providing novel insights for the optimization and design of CDT agents.

关键词： CuPd nanoparticle ferroptosis chemodynamic therapy valance tailoring tumor therapy

Magnetic resonance imaging-based deep learning model to predict multiple firings in double-stapled colorectal anastomosis

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World Journal of Gastroenterology 2023年第3期29卷 536-548页

作者： Zheng-Hao Cai Qun Zhang Zhan-Wei Fu Abraham Fingerhut jing-Wen tan Lu Zang Feng Dong Shu-Chun Li Shi-Lin Wang Jun-Jun Ma Department of General Surgery Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China Shanghai Minimally Invasive Surgery Center Shanghai 200025China School of Electronic Information and Electrical Engineering Shanghai Jiao Tong UniversityShanghai 201100China Department of Radiology Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China

BACKGROUND Multiple linear stapler firings during double stapling technique(DST)after laparoscopic low anterior resection(LAR)are associated with an increased risk of anastomotic leakage(AL).However,it is difficult to predict preoperatively the need for multiple linear stapler cartridges during DST *** To develop a deep learning model to predict multiple firings during DST anastomosis based on pelvic magnetic resonance imaging(MRI).METHODS We collected 9476 MR images from 328 mid-low rectal cancer patients undergoing LAR with DST anastomosis,which were randomly divided into a training set(n=260)and testing set(n=68).Binary logistic regression was adopted to create a clinical model using six *** sequence of fast spin-echo T2-weighted MRI of the entire pelvis was segmented and ***-image and clinical-image integrated deep learning models were constructed using the mask region-based convolutional neural network segmentation tool and three-dimensional convolutional ***,specificity,accuracy,positive predictive value(PPV),and area under the receiver operating characteristic curve(AUC)was calculated for each *** The prevalence of≥3 linear stapler cartridges was 17.7%(58/328).The prevalence of AL was statistically significantly higher in patients with≥3 cartridges compared to those with≤2 cartridges(25.0%vs 11.8%,P=0.018).Preoperative carcinoembryonic antigen level>5 ng/mL(OR=2.11,95%CI 1.08-4.12,P=0.028)and tumor size≥5 cm(OR=3.57,95%CI 1.61-7.89,P=0.002)were recognized as independent risk factors for use of≥3 linear stapler *** performance was better with the integrated model(accuracy=94.1%,PPV=87.5%,and AUC=0.88)compared with the clinical model(accuracy=86.7%,PPV=38.9%,and AUC=0.72)and the image model(accuracy=91.2%,PPV=83.3%,and AUC=0.81).CONCLUSION MRI-based deep learning model can predict the use of≥3 linear stapler cartridges during DST anastomosis in laparoscopic LAR *** model might h

关键词： Deep learning Image-reading artificial intelligence Magnetic resonance imaging Predictive model Double stapling technique Linear stapler Rectal cancer Laparoscopic surgery Low anterior resection Anastomotic leakage

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脑卒中后抑郁风险预测模型的研究进展

中华神经医学杂志 2022年第5期21卷 523-527页

作者：谭静刘宁遵义医科大学珠海校区护理学系珠海519041

脑卒中后抑郁(PSD)是脑卒中后常见的并发症,严重影响患者生存质量,早发现、早治疗可明显改善脑卒中患者的抑郁状况。现有的PSD风险预测模型多基于临床危险因素、患者社会心理因素、影像学因素、生物学标志物及中医证候因素建立,本文现... 详细信息

脑卒中后抑郁(PSD)是脑卒中后常见的并发症,严重影响患者生存质量,早发现、早治疗可明显改善脑卒中患者的抑郁状况。现有的PSD风险预测模型多基于临床危险因素、患者社会心理因素、影像学因素、生物学标志物及中医证候因素建立,本文现围绕模型的建立过程、预测性能、不足之处及发展趋势综述如下。

关键词：脑卒中后抑郁风险因素预测模型

绿色城区的建设路径探索——以粤港澳大湾区N片区为例

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城市发展研究 2023年第8期30卷 18-25页

作者：王昆谭静中国城市规划设计研究院绿色城市研究所

以绿色城区的建设路径为研究内容。首先，梳理绿色城区的内涵，明确绿色城区建设的要求和重点。其次，以粤港澳大湾区N片区为例，研究该绿色城区的基础条件和示范性，开展绿色城区建设的总体目标、指标体系和技术谱系，并针对现状特征... 详细信息

以绿色城区的建设路径为研究内容。首先，梳理绿色城区的内涵，明确绿色城区建设的要求和重点。其次，以粤港澳大湾区N片区为例，研究该绿色城区的基础条件和示范性，开展绿色城区建设的总体目标、指标体系和技术谱系，并针对现状特征和发展目标，明确包含生态基础设施建设、多元混合街区打造、立体绿色交通组织、清洁循环设施供给、绿色低碳建筑建造、多场景智慧化运营在内的六项绿色城区建设主要任务，明确各项任务的核心举措，以期为我国绿色城区建设提供经验借鉴。

关键词：绿色城区城市建设技术体系粤港澳大湾区

三种机器学习算法预测心力衰竭死亡的价值研究

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医学信息 2024年第11期37卷 11-15页

作者：陈晓彤岑梓熹谭静仪栾雅彭师师严波何震广州新华学院健康学院江苏科技大学材料工程学院

目的用机器学习三种不同算法建立心力衰竭分类预测模型，比较模型的准确率，并分析心力衰竭死亡事件重要性特征，对人群尽早发现和实施介入措施提供援助，努力提高人们的健康水平和生活质量。方法使用Kaggle平台发布的心力衰竭数据集... 详细信息

目的用机器学习三种不同算法建立心力衰竭分类预测模型，比较模型的准确率，并分析心力衰竭死亡事件重要性特征，对人群尽早发现和实施介入措施提供援助，努力提高人们的健康水平和生活质量。方法使用Kaggle平台发布的心力衰竭数据集，通过缺失值填充法、数据标准化处理、SMOTE方法进行数据预处理。基于随机森林、C4.5、AdaBoost算法建立心力衰竭预测模型。使用性能评估指标混淆矩阵、ROC曲线、均方根误差以及均值绝对误差评估评价模型性能。结果 PermutationImportance给出的变量重要性排序中，血清肌酐水平、年龄、血清钠离水平排序靠前。三种模型中，随机森林模型准确率为85%，精确率为81%，召回率为68%;C4.5模型准确率为83%，精确率为80%，召回率为63%;AdaBoost模型准确率为80%，精确率为71%，召回率为63%。结论基于所用数据集，随机森林模型优于C4.5模型与AdaBoost模型，心力衰竭死亡风险预测模型能为心力衰竭早期预防控制及诊断提供参考依据。

关键词：心力衰竭死亡预测模型 C4.5 随机森林 AdaBoost

The IDD Transcription Factors:Their Functions in Plant Development and Environmental Response

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Phyton-International Journal of Experimental Botany 2024年第1期93卷 63-79页

作者： jing Liu Defeng Shu Zilong tan Mei Ma Huanhuan Yang Ning Guo Shipeng Li Dayong Cui School of Life Sciences Qilu Normal UniversityJinan250200China School of Life Sciences Shandong Normal UniversityJinan250014China

INDETERMINATE-DOMAIN proteins(IDDs)are a plant-specific transcription factor family characterized by a conserved ID domain with four zinc finger *** studies have demonstrated that IDDs coordinate a diversity of physiological processes and functions in plant growth and development,including floral transition,plant architecture,seed and root development,and hormone *** this review,we especially summarized the latest knowledge on the functions and working models of IDD members in Arabidopsis,rice,and maize,particularly focusing on their role in the regulatory network of biotic and abiotic environmental responses,such as gravity,temperature,water,and *** these mechanisms underlying the function of IDD proteins in these processes is important for improving crop yields by manipulating their ***,the review offers valuable insights into the functions and mechanisms of IDD proteins in plants,providing a foundation for further research and potential applications in agriculture.

关键词： INDETERMINATE DOMAIN flowering time root development shoot gravitropism plant immunity hormonal signaling environmental responses