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Chinese Chemical Letters 2024年第3期35卷 384-388页

作者： Yi-jing Li Lingze Zhang Ming-Hao Pang Pei-Pei Yang Lu-Ming Guo Kuo Zhang Da-Yong Hou Lei Wang Hao Wang Hui cao State Key Laboratory for Advanced Metals and Materials School of Materials Science and EngineeringUniversity of Science and Technology BeijingBeijing 100083China CAS Center for Excellence in Nanoscience CAS Key Laboratory for Biomedical Effects of Nanomaterials NanosafetyNational Center for Nanoscience and Technology(NCNST)Beijing 100190China

Peptide drugs are known for their high biological safety.However,compared with small molecule drugs,peptide drugs are easily oxidized and hydrolyzed as well as short in half-life.Herein,inspired by the long circulation of albumin in blood,we screened albumin binding peptides(ABPs)from a one-bead one-compound(OBOC)peptide library to increase the half-life of peptide drugs.Beads displaying random peptides were screened using fluorescent labeled human serum albumin.Fluorescent beads with specific binding to albumin were isolated for sequencing.The selected ABPs can effectively bind to albumin,thus possessing the long circulation of albumin.The dissociation constant(K_(D))of ABPs to albumin is up to 1×10^(-8)mol/L.Once one of ABPs(ABP2)was coupled to triptorelin,the circulation half-life of triptorelin in mice was significantly prolonged to 263.50 h much longer than that of triptorelin alone(179.07 h).In addition,the combination therapy using ABP-conjugated triptorelin and doxorubicin(DOX)can effectively inhibit the proliferation of tumor cells in mice.The OBOC screening strategy and resulting ABPs showed great potential for enhancing the delivery efficiency of peptide drugs.

关键词： One-bead one-compound Combinatorial library Albumin binding Drug delivery Triptorelin

Abnormal function of EPHA2/p.R957P mutant in congenital cataract

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International Journal of Ophthalmology(English edition) 2024年第6期17卷 1007-1017页

作者： jing-Jin Zhang Zong-Fu cao Bi-Ting Zhou Ju-Hua Yang Zhong Li Shuang Lin Xiao-Le Chen Nan-Wen Zhang Qin Ye Xu Ma Yi-Hua Zhu Department of Ophthalmology the First Affiliated Hospital of Fujian Medical UniversityFujian Institute of OphthalmologyFujian Provincial Clinical Medical Research Center of Eye Diseases and OptometryFuzhou 350005Fujian ProvinceChina Department of Ophthalmology National Regional Medical CenterBinhai Campus of the First Affiliated HospitalFujian Medical UniversityFuzhou 350005Fujian ProvinceChina Chinese Academy of Medical Sciences&Peking Union Medical College Beijing 100000China National Human Genetic Resources Center National Research Institute for Family PlanningBeijing 100081China Department of Bioengineering and Biopharmaceutics School of PharmacyFujian Medical UniversityFuzhou 350004Fujian ProvinceChina

AIM:To identify genetic defects in a Chinese family with congenital posterior polar cataracts and assess the pathogenicity.METHODS:A four-generation Chinese family affected with autosomal dominant congenital cataract was recruited.Nineteen individuals took part in this study including 5 affected and 14 unaffected individuals.Sanger sequencing targeted hot-spot regions of 27 congenital cataract-causing genes for variant discovery.The pathogenicity of the variant was evaluated by the guidelines of American College of Medical Genetics and InterVar software.Confocal microscopy was applied to detect the subcellular localization of fluorescence-labeled ephrin type-A receptor 2(EPHA2).Co-immunoprecipitation assay was implemented to estimate the interaction between EphA2 and other lens membrane proteins.The mRNA and protein expression were analyzed by reverse transcription-polymerase chain reaction(qRT-PCR)and Western blotting assay,respectively.The cell migration was analyzed by wound healing assay.Zebrafish model was generated by ectopic expression of human EPHA2/p.R957P mutant to demonstrate whether the mutant could cause lens opacity in vivo.RESULTS:A novel missense and pathogenic variant c.2870G>C was identified in the sterile alpha motif(SAM)domain of EPHA2.Functional studies demonstrated the variant’s impact:reduced EPHA2 protein expression,altered subcellular localization,and disrupted interactions with other lens membrane proteins.This mutant notably enhanced human lens epithelial cell migration,and induced a central cloudy region and roughness in zebrafish lenses with ectopic expression of human EPHA2/p.R957P mutant under differential interference contrast(DIC)optics.CONCLUSION:Novel pathogenic c.2870G>C variant of EPHA2 in a Chinese congenital cataract family contributes to disease pathogenesis.

关键词： congenital cataract EPHA2 missense variant function analysis

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土地金融化、资源错配与企业全要素生产率

金融评论 2024年第1期16卷 71-85,155页

作者：曹婧中国社会科学院金融研究所

随着地方政府土地融资模式从土地财政转向土地金融,土地的金融属性成为评估“以地谋发展”经济贡献的关键变量。本文使用2007~2015年全国税收调查微观数据和地级市土地融资数据,基于土地和金融资源双重错配的视角,考察土地金融化对企业... 详细信息

随着地方政府土地融资模式从土地财政转向土地金融,土地的金融属性成为评估“以地谋发展”经济贡献的关键变量。本文使用2007~2015年全国税收调查微观数据和地级市土地融资数据,基于土地和金融资源双重错配的视角,考察土地金融化对企业全要素生产率的影响及其作用机制。研究发现,融资平台债务扩张引致的土地金融化显著抑制企业全要素生产率的提升,这一效应在土地价格扭曲严重和非标债务占比较高的地区,以及非国有企业样本中表现得更为明显。机制分析表明,土地金融化一方面通过强化土地引资竞争,造成低生产率企业以低成本购置大量工业用地;另一方面使金融资源更多流向具有信贷优势的国有企业和以低成本占用土地的低生产率企业,由此产生的土地和金融资源配置扭曲不利于企业全要素生产率提高。本文不仅拓展了已有文献关于土地出让影响资源错配与生产率损失的研究,也为深化要素市场化配置改革和推动经济高质量发展提供了决策参考。

关键词：土地财政土地金融资源错配全要素生产率

Sciadopitysin exerts anticancer effects on HepG2 hepatocellular carcinoma cells by regulating reactive oxygen species-mediated signaling pathways

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BIOCELL 2024年第7期48卷 1055-1069页

作者： YAN-NAN LI YUN-HONG XIU YAN-JUN TANG jing-LONG cao WEN-SHUANG HOU AN-QI WANG TIAN-ZHU LI CHENG-HAO JIN Department of Biochemistry and Molecular Biology Heilongjiang Bayi Agricultural UniversityDaqingChina Hemodialysis Center Daqing Oilfield General HospitalDaqingChina Department of Food Science and Engineering Heilongjiang Bayi Agricultural UniversityDaqingChina Department of Molecular Biology Chifeng UniversityChifengChina National Coarse Cereals Engineering Research Center DaqingChina

Objectives:Sciadopitysin(SP)is aﬂavonoid in Ginkgo biloba that exhibits various pharmacological activities.This study aimed to investigate its antitumor effects and the underlying molecular mechanism of SP in hepatocellular carcinoma(HCC)cells.Methods:Network pharmacology was used for target prediction analysis.Cell Counting Kit-8(CCK-8)assay was used to test the cell viability.Flow cytometry was used to test the cell cycle distribution,apoptosis status,and reactive oxygen species(ROS)levels.Transwell and wound-healing assay was used to test the migration effect of SP on HepG2 cells.Western Blot assay was used to test the expression levels of proteins.Results:Network pharmacology analysis results showed that the mitogen-activated protein kinase(MAPK)and other signaling pathways are involved in the SP anti-HCC biological process.CCK-8 assay results demonstrated that SP showed an obvious killing effect on three types of HCC cells and low cytotoxic effect on normal cells.Western Blot andﬂow cytometry results showed that SP regulated MAPK/signal transducer and activator of transcription 3(STAT3)/nuclear factor kappa-B(NF-κB)signaling pathway to induce mitochondrion-dependent apoptosis in HepG2 cells.Additionally,SP can arrest the G0/G1 phase cell cycle via the protein kinase B(AKT)/p21/p27/cyclin-dependent kinase(CDK)/Cyclin signaling pathway.Wound healing and transwell assays showed that SP inhibited cell motility and invasion through the AKT/glycogen synthase kinase3β(GSK-3β)/vimentin/β-catenin signaling pathway.Conclusion:Theseﬁndings demonstrated that SP induced mitochondrion-dependent apoptosis,arrested cell cycle in the G0/G1 phase,and inhibited cell migration by regulating the ROS-mediated signaling pathway in HepG2 cells.Thus,SP could serve as a therapeutic agent for the treatment of human HCC.

关键词： Sciadopitysin Hepatocellular carcinoma Apoptosis Cell cycle Cell migration

Ferroptosis: A therapeutic opportunity of inflammatory bowel disease

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中华医学杂志(英文版) 2024年第7期137卷 874-876页

作者： Ye Yulin Liu Limin jing Yang Yao Shuangzhe Yang Mo Dai Xin Piao Meiyu Xu Xin Feng Zelin Wang Xiaoli Liu Yifei Miao Junming Gao Xingjie Yu Qingxiang cao Xiaocang Department of Gastroenterology and Hepatology Tianjin Medical University General Hospital Tianjin Medical University Tianjin 300070 China Department of Biochemistry and Molecular Biology School of Basic Medical Science

Recent progress in porous Mg-based foam preparation approaches:effect of processing parameters on structure and mechanical property

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Journal of Iron and Steel Research(International) 2022年第3期29卷 371-402页

作者： Solomon-Oshioke Agbedor Dong-hui Yang jing cao Jian-qing Chen Bassiouny Saleh Chao Qiu Lei Wang jing-hua Jiang Ai-bin Ma College of Mechanics and Materials Hohai UniversityNanjing211100JiangsuChina Suqian Research Institute of Hohai University Suqian223814JiangsuChina Ocean and Offshore Engineering Research Institute of Hohai University Nantong226019JiangsuChina

Porous metals are a class of cellular materials with lightweight and unique mechanical,electrical,thermal,physical and acoustic characteristics.Magnesium and magnesium alloy foams have exhibited excellent advantages.In particular,open-cell Mg-based foams(porous Mg/Mg alloy foams)have been used for bioresorbable implants,CO_(2)trapping systems,filters,heat exchangers,absorbent panels and many other applications.While significant progress has been taken in producing porous Mg-based foams with good structure-property relations,but with a large number of different processing parameters,different mechanical properties and pore morphologies of each porous Mg-based foam,it is essential to understand the individual effects of each aspect of the parameters.Therefore,the present article summarized the effects of available processing parameters on the structure and mechanical properties of the porous Mg-based foams.Finally,the future perspectives to enhance the structure and properties of porous Mg/Mg alloy foams were discussed.

关键词： Porous Mg alloy Powder processing Sintering Liquid metal infiltration Mechanical property

Poisoning effect of polyvinyl chloride on the catalytic pyrolysis of mixed plastics over zeolites

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Science China Chemistry 2024年第7期67卷 2265-2273页

作者： Jiangchun Yuan Wenjie Wang Weixiao Sun Zhirong Yang Yueqiang cao Wenyao Chen Xiaohu Ge Gang Qian Xiang Feng Xuezhi Duan Xinggui Zhou Kevin M.Van Geem jing Zhang State Key Laboratory of Chemical Engineering East China University of Science and TechnologyShanghai 200237China School of Chemical Engineering Zhengzhou UniversityZhengzhou 450001China Laboratory for Chemical Technology(LCT) Department of MaterialsTextiles and Chemical EngineeringFaculty of Engineering&ArchitectureGhent UniversityZwijnaarde B-9052Belgium State Key Lab of Heavy Oil Processing China University of PetroleumQingdao 266580China

Nowadays,the chemical recycling is applied for only 1%of total waste plastics,largely due to contaminants in plastic waste and difficulty in product control.As the major contaminant,polyvinyl chloride(PVC)often forms corrosive hydrogen chloride(HCl)during the chemical recycling,which may cause severe catalyst deactivation and equipment damage.However,the investigation on catalytic pyrolysis(the major route for plastics chemical recycling)of the PVC containing mixed plastics has been rarely reported.Here,catalytic co-pyrolysis of PVC and polyethylene(PE)was studied over an aromatization catalyst,Pt/ZSM-5,since the basic building block aromatics are desired products from plastics chemical recycling.The poisoning effect of PVC vapor on the catalyst stability was explored by collective efforts of thorough product analysis and catalyst characterization.It was found that the HCl evolving from PVC has an autocatalytic effect that promotes the scission of dehydrochlorinated PVC,resulting in the high yield of benzene and acetylene from PVC.On the other hand,the presence of PVC suppressed the aromatics formation from PE,largely due to the poisoning effect of PVC-derived HCl on the Pt/ZSM-5.The deactivation is irreversible as evidenced by the decreased zeolite crystallinity and the loss of strong acid sites that are key to the aromatization,possibly due to the removal of framework Al upon the interaction with HCl.The modification with octadecylphosphonic acid only slightly alleviated the PVC poisoning effect.The insights on the PVC poisoning of zeolite catalysts provided in this work may guide the process design of chemical recycling of PVC containing waste plastics.

关键词： polyvinyl chloride polyethylene zeolite aromatics deactivation

WNT5A Regulated by miR154-5p is Associated with Angiopoiesis and Invasion of Placental Site Trophoblastic Tumor (PSTT)

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Biomedical and Environmental Sciences 2024年第4期37卷 423-427页

作者： ZHANG Sai CHEN Zhi Xian ZHANG Mo DU Yan ZHOU Jia Yi WU jing YU Yin Hua cao Qi ZHAO Hong Bo Obstetrics and Gynecology Hospital Fudan UniversityShanghai 200011China Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Shanghai 200011China

Placental site trophoblastic tumor(PSTT),a rare gestational trophoblastic neoplasm(GTN),exhibits slow growth,late-onset metastasis,and chemotherapy insensitivity[1,2].The invasive capacity of trophoblasts induces angiogenesis in PSTT,yet the specific mechanism remains unclear[3].PSTT is typically resistant to chemotherapy and hysterectomy stands as the primary therapeutic choice for patients with an overall cure rate of approximately 75%-80%[4,5].Therefore,investigating the precise emergence and progression of PSTT is crucial,aiming to identify novel targets for diagnosis and therapy.

关键词： chemotherapy diagnosis metastasis

Cancer statistics for young adults aged 20 to 49 years in China from 2000 to 2017:a population-based registry study

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Science China(Life Sciences) 2024年第4期67卷 711-719页

作者： Yi Teng Changfa Xia He Li Maomao cao Fan Yang Xinxin Yan Siyi He Mengdi cao Shaoli Zhang Qianru Li Wanqing Chen Office of Cancer Screening National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China

An increasing cancer incidence among adults younger than 50 years has been reported for several types of cancer in multiple countries.We aimed to report cancer profiles and trends among young adults in China.Data from the China Cancer Registry Annual Report were used to estimate incidence and mortality among young adults(ages 20–49 years)in China in 2017,and an age-period-cohort model was employed to estimate the average annual percent change(AAPC)in incidence and mortality from 2000 to 2017.All 25 cancer types were grouped into obesity-or overweight-associated cancers(12 cancer types)and additional cancers(13 cancer types).In 2017,there were 681,178new cases and 214,591 cancer deaths among young adults in China.Among young adults,the most common cancers were thyroid,breast,cervical,liver,lung,and colorectal cancer,and the leading causes of cancer deaths were liver,lung,cervical,stomach,breast,and colorectal cancer.From 2000 to 2017,the cancer incidence increased for all cancers combined among young adults,with the highest AAPC(1.46%)for adults aged 20–24 years,while cancer mortality decreased,with the highest AAPC(-1.63%)for those aged 35–39 years.In conclusion,the cancer incidence in China has increased among young adults,while cancer mortality has decreased for nearly all ages.Cancer control measures,such as obesity control and appropriate screening,may contribute to reducing the increasing cancer burden among young adults.

关键词： young adults incidence mortality trends China

An Improved Parameter Dimensionality Reduction Approach Based on a Fast Marching Method for Automatic History Matching

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Fluid Dynamics & Materials Processing 2022年第3期18卷 609-628页

作者： Hairong Zhang Yongde Gao Wei Li Deng Liu jing cao Luoyi Huang Xun Zhong Southern Marine Science and Engineering Guangdong Laboratory Zhanjiang Bay LaboratoryZhanjiang524000China Zhanjiang Branch of China National Offshore Oil Corporation Zhanjiang524000China College of Petroleum Engineering Yangtze UniversityWuhan430100China

History matching is a critical step in reservoir numerical simulation algorithms.It is typically hindered by difficulties associated with the high-dimensionality of the problem and the gradient calculation approach.Here,a multi-step solving method is proposed by which,first,a Fast marching method(FMM)is used to calculate the pressure propagation time and determine the single-well sensitive area.Second,a mathematical model for history matching is implemented using a Bayesian framework.Third,an effective decomposition strategy is adopted for parameter dimensionality reduction.Finally,a localization matrix is constructed based on the single-well sensitive area data to modify the gradient of the objective function.This method has been verified through a water drive conceptual example and a real field case.The results have shown that the proposed method can generate more accurate gradient information and predictions compared to the traditional analytical gradient methods and other gradient-free algorithms.

关键词： History matching parameter dimensionality reduction sensitive area gradient correction