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A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops

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Food Science and Human Wellness 2024年第1期13卷 183-190页

作者： jinbin wang Yu wang Xiuwen Hu Yifan Chen Wei Jiang Xiaofeng Liu Juan Liu Lemei Zhu Haijuan Zeng Hua Liu Key Laboratory of Agricultural Genetics and Breeding Institute of Biotechnology ResearchShanghai Academy of Agricultural SciencesShanghai 201106China Crops Ecological Environment Security Inspection and Supervision Center(Shanghai) Ministry of Agriculture and Rural AffairsShanghai 201106China School of Life Science and Engineering Lanzhou University of TechnologyLanzhou 730050China School of Public Health Changsha Medical UniversityChangsha 410219China Academician Workstation Changsha Medical UniversityChangsha 410219China

Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and *** this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)*** which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked *** diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,*** constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high *** addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other *** conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.

关键词： Genetically modifi ed crops On-site detection Lateral fl ow test strips Dual recombinase polymerase amplification (RPA)

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Effect of interfacial delamination on coating crack in thick diamond-like carbon coatings under indentation

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Acta Mechanica Sinica 2020年第2期36卷 524-535页

作者： Zhongbao wang Xiangli Zhong Limei Jiang Fugang Qi Xiaoping Ouyang jinbin wang Bin Liao Jun Luo Key Laboratory of Low Dimensional Materials and Application Technology School of Materials Science and EngineeringXiangtan UniversityXiangtan 411105China College of Nuclear Science and Technology Beijing Normal UniversityBeijing 100875China

Coating crack and interfacial delamination are recognized as two critical factors inducing spallation of thick diamond-like carbon(DLC)*** effect of the two factors is found to dramatically accelerate the failure of thick DLC ***,there are few reports on the effect of interfacial delamination on coating *** this work,in order to investigate the evolution of the coating crack and interfacial delamination,as well as the effect of interfacial delamination on coating crack,a finite element model that combines the bilinear cohesive zone model and the extended finite element method(XFEM)is *** is found that the occurrence of interfacial delamination triggers a second expansion of coating *** influencing the degree of interfacial delamination on coating crack can be modulated by coating thickness and coating elastic *** the coating thickness increases,the length of interfacial delamination increases,and thus the propagation of coating crack is *** contrast,the increase of coating elastic modulus could reduce the length of interfacial delamination,which consequently weakens its influence on the propagation of coating crack.

关键词： Coating crack Interfacial delamination Interaction Indentation test Cohesive zone model

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基于核酸靶标免扩增CRISPR/Cas体系的分子速测技术研究进展

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中国生物工程杂志 2024年第6期44卷 104-115页

作者：李柚张岩雒嘉炜徐丹红马娟高梦祥刘华曾海娟王金斌长江大学生命科学学院荆州434022 上海市农业科学院生物技术研究所农业农村部农业转基因生物安全评价(环境)重点实验室上海201106

快速准确的分子诊断在食品安全、疾病诊断、环境监测等生命科学领域发挥重要作用。成簇规则间隔短回文重复序列(clustered regularly interspaced short palindromic repeats,CRISPR)及相关蛋白(CRISPR-associated protein,Cas)组成的CRISPR/Cas系统因具有高灵敏度、高特异性、便捷性及快速等优势,已成为精确检测核酸靶标的重要分子诊断工具。基于CRISPR/Cas系统的检测通过预扩增步骤提高靶标浓度,但也随之带来气溶胶污染、操作复杂和检测周期长等问题。因此需要积极开发更多免扩增策略,在最大限度保证灵敏度的同时,一步式实现对靶标的检测。综述了免扩增CRISPR系统的重要性,重点阐述了反应影响因素及相关策略对体系的优化作用,以及数字芯片法、表面增强拉曼光谱法和电化学金属电极法对信号放大效果的前沿进展,并对CRISPR/Cas系统的即时检测应用进行展望,以期为免扩增CRISPR/Cas检测系统的发展提供理论指导。

关键词：成簇规则间隔短回文重复序列(CRISPR) 免扩增检测体系优化信号放大

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碲锌镉晶体的铟碲共掺杂退火研究

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现代应用物理 2024年第2期15卷 26-33,41页

作者：戴伟雷宇李州王许琛宋宏甲钟向丽王金斌湘潭大学材料科学与工程学院湖南湘潭411105

针对生长态碲锌镉晶体缺陷密度大和电学性能无法满足室温核辐射探测器的制备要求等问题,研究了铟碲共掺杂退火对碲锌镉晶体碲夹杂和电学性能的影响。利用分子动力学方法模拟了不同温度下铟原子在碲锌镉晶体中的扩散过程,获得了铟原子的... 详细信息

针对生长态碲锌镉晶体缺陷密度大和电学性能无法满足室温核辐射探测器的制备要求等问题,研究了铟碲共掺杂退火对碲锌镉晶体碲夹杂和电学性能的影响。利用分子动力学方法模拟了不同温度下铟原子在碲锌镉晶体中的扩散过程,获得了铟原子的扩散系数表达式,计算了铟原子扩散至碲锌镉晶体所需理论时长,在此基础上开展了铟碲共掺杂退火实验,进一步优化了退火工艺。实验结果表明,铟碲共掺杂退火70 h的碲锌镉晶体碲夹杂密度下降至27.61 mm^(-2),体电阻率接近1011Ω·cm、漏电流低于4 nA(400 V),电学性能达到核辐射探测器应用要求。

关键词：碲锌镉分子动力学模拟退火碲夹杂电学性能

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土壤真菌群落和潜在功能对施加外源Bt毒素的响应

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土壤学报 2024年第3期61卷 848-861页

作者：李玉洁葛蕾胡聪王璐瑶郭官清武国干王翠宋丽莉孙宇王金斌曾海娟徐勤青全哲学李鹏上海海洋大学食品学院上海201306 上海市农业科学院生物技术研究所农业农村部农业转基因生物安全评价(环境)重点实验室上海市农业遗传育种重点实验室上海201106 山东省农业技术推广中心济南250003 复旦大学生命科学学院上海200433

转Bt(Bacillus thuringiensis)基因植物和Bt重组菌生物农药释放的Bt毒素是一类具有生物毒性的潜在环境外源污染物,Bt毒素环境行为和生态效应是转基因植物和植物用转基因微生物安全风险评价的重要内容,但是外源Bt毒素对土壤真菌群落和潜... 详细信息

转Bt(Bacillus thuringiensis)基因植物和Bt重组菌生物农药释放的Bt毒素是一类具有生物毒性的潜在环境外源污染物,Bt毒素环境行为和生态效应是转基因植物和植物用转基因微生物安全风险评价的重要内容,但是外源Bt毒素对土壤真菌群落和潜在功能的影响还不清楚。以施加不同浓度Bt毒素处理土壤和未施加Bt毒素对照土壤为研究对象,分析Bt毒素在土壤中的持留动态;同时采用真菌18S rRNA基因高通量测序技术,分析施加Bt毒素对土壤真菌群落和功能多样性的影响。结果表明,Bt毒素施加量和培养时间均可以显著影响土壤真菌群落组成,且随着Bt毒素施加量增加和土壤培养时间延长,土壤真菌群落差异性逐渐变大。施加Bt毒素提高了土壤真菌群落香农指数和关联网络的负相关性比例及模块数,因而没有对土壤真菌群落的多样性和稳定性产生负面影响。上述结果表明,评估Bt毒素的环境行为及微生态效应要关注Bt毒素施加量及其长期影响。随着Bt毒素施加量增加,Phymatotrichopsis、Homalogastra、Geosmithia和Apiotrichum等真菌以及参与蛋白质降解、碳素代谢和磷素代谢的功能基因编码酶相对丰度显著升高,推测上述真菌物种和潜在功能参与了Bt毒素在土壤中的降解和转化过程。研究结果为转Bt基因植物、Bt重组菌生物农药以及Bt毒素的生态安全风险评价提供了科学参考和理论依据。

关键词： Bt毒素转Bt基因植物 Bt菌生物农药群落多样性生物安全风险评价

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聚天门冬氨酸酯防水涂料性能研究

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中国建筑防水 2024年第2期 11-13页

作者：夏伟伟辛伟马晨关敏杰王晋斌北新防水(河南)有限公司河南许昌461502

介绍了一种聚天门冬氨酸酯防水涂料的制备方法,并测试了该涂料在标准条件、浸水处理、热处理后的主要性能。结果表明:该防水涂料的性能符合防水通用规范的要求,且浸水处理后力学性能保持率均在96%以上,28 d吸水率仅为2.1%,粘结强度保持... 详细信息

介绍了一种聚天门冬氨酸酯防水涂料的制备方法,并测试了该涂料在标准条件、浸水处理、热处理后的主要性能。结果表明:该防水涂料的性能符合防水通用规范的要求,且浸水处理后力学性能保持率均在96%以上,28 d吸水率仅为2.1%,粘结强度保持率在95%以上;热处理后力学性能保持率均在96%以上。

关键词：聚天门冬氨酸酯防水涂料浸水处理热处理力学性能吸水率

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Localized electric-field-enhanced low-light detection by a 2D SnS visible-light photodetector

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Chinese Physics B 2021年第5期30卷 655-660页

作者： Hao Wen Li Xiong Congbing Tan Kaimin Zhu Yong Tang jinbin wang Xiangli Zhong School of Materials Science and Engineering Xiangtan UniversityXiangtan 411105HunanChina

Due to their excellent carrier mobility,high absorption coefficient and narrow bandgap,most 2D IVA metal chalcogenide semiconductors(GIVMCs,metal=Ge,Sn,Pb;chalcogen=S,Se)are regarded as promising candidates for realizing high-performance *** synthesized high-quality two-dimensional(2D)tin sulfide(SnS)nanosheets using the physical vapor deposition(PVD)method and fabricated a 2D SnS visible-light *** photodetector exhibits a high photoresponsivity of 161 A·W-1 and possesses an external quantum efficiency of 4.45×10^(4)%,as well as a detectivity of 1.15×10^(9) Jones under 450 nm blue light ***,under poor illumination at optical densities down to 2 mW·cm^(-2),the responsivity of the device is higher than that at stronger optical *** suggest that a photogating effect in the 2D SnS photodetector is mainly responsible for its low-light *** and impurities in 2D SnS can trap carriers and form localized electric fields,which can delay the recombination process of electron-hole pairs,prolong carrier lifetimes,and thus improve the low-light *** work provides design strategies for detecting low levels of light using photodetectors made of 2D materials.

关键词： two-dimensional SnS photogating effect low-light detection

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Chromatin architectural alterations due to null mutation of a major CG methylase in rice

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Journal of Integrative Plant Biology 2022年第12期64卷 2396-2410页

作者： jinbin wang Xiaochong Li Qianli Dong Changping Li Juzuo Li Ning Li Baoxu Ding Xiaofei wang Yanan Yu Tianya wang Zhibin Zhang Yiyang Yu Man Lang Zixian Zeng Bao Liu Lei Gong Key Laboratory of Molecular Epigenetics of the Ministry of Education(MOE) Northeast Normal UniversityChangchun 130024China State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesBejig 100101China Hainan Yazhou Bay Seed Lab Sanya 572025China Department of Biological Science College of Life ScienceSichuan Normal UniversityChengdu 610101China

Associations between 3D chromatin architectures and epigenetic modifications have been characterized in ***,any impact of DNA methylation on chromatin architecture in plants is understudied,which is confined to Arabidopsis *** plant species differ in genome size,composition,and overall chromatin packing,it is unclear to what extent findings from *** hold in other ***,the incomplete chromatin architectural profiles and the low-resolution high-throughput chromosome conformation capture(Hi-C)data from *** have hampered characterizing its subtle chromatin structures and their associations with DNA *** constructed a high-resolution Hi-C interaction map for the null OsMET1-2(the major CG methyltransferase in rice)mutant(osmet1-2)and isogenic wild-type rice(WT).Chromatin structural changes occurred in osmetl-2,including intra-/inter-chromosomal interactions,compartment transition,and topologically associated domains(TAD)*** findings provide novel insights into the potential function of DNA methylation in TAD formation in rice and confirmed DNA methylation plays similar essential roles in chromatin packing in A,thaliana and rice.

关键词： 3D chromatin architectures DNA methylation Hi-C osmet1-2 mutant

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Electrically driven motion, destruction, and chirality change of polar vortices in oxide superlattices

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Science China(Physics,Mechanics & Astronomy) 2022年第3期65卷 77-84页

作者： Pan Chen Congbing Tan Zhexin Jiang Peng Gao Yuanwei Sun Lifen wang Xiaomei Li Ruixue Zhu Lei Liao Xu Hou Ke Qu Ning Li Xiaomin Li Zhi Xu Kaihui Liu Wenlong wang jinbin wang Xiaoping Ouyang Xiangli Zhong Jie wang Xuedong Bai Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China International Center for Quantum Materials Electron Microscopy LaboratorySchool of PhysicsPeking UniversityBeijing 100871China Hunan Provincial Key Laboratory of Intelligent Sensors and Advanced Sensor Materials School of Physics and ElectronicsHunan University of Science and TechnologyXiangtan 411201China Department of Engineering Mechanics Zhejiang UniversityHangzhou 310027China Collaborative Innovation Center of Quantum Matter Beijing 100871China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100190China Songshan Lake Materials Laboratory Dongguan 523808China State Key Laboratory for Artificial Microstructure&Mesoscopic Physics School of PhysicsPeking UniversityBeijing 100871China School of Materials Science and Engineering Xiangtan UniversityXiangtan 411105China Key Laboratory of Soft Machines and Smart Devices of Zhejiang Province Zhejiang UniversityHangzhou 310027China

Topological polar vortices, which are electric analogs of magnetic objects, present great potential in applications of future nanoelectronics because of their nanometer size, anomalous dielectric response, and chirality. To enable the functionalities, it is prerequisite to manipulate the polar states and chirality by using external stimuli. Here, we probe the evolutions of polar state and chirality evolutions of topological polar vortices in Pb TiO;/Sr TiO;superlattices under an electric field by using atomically resolved in situ scanning transmission electron microscopy and phase-field simulations. We find that, under electric field, the chiral vortex cores can be moved laterally to form close-pair structures, transform into a/c domain stripes, and finally become a nonchiral c-domain. Such transition is reversible and spontaneous after bias removal. Interestingly, during switching and backswitching events, the vortex rotation can be changed, offering a potential strategy to manipulate vortex chirality. The revealed dynamic behavior of individual polar vortices at the atomic scale provides fundamentals for future device applications.

关键词： chirality polar vortex ferroelectrics in situ transmission electron microscopy phase-field simulations

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Characterization of domain distributions by second harmonic generation in ferroelectrics

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npj Computational Materials 2018年第1期4卷 339-345页

作者： Yuan Zhang Yi Zhang Quan Guo Xiangli Zhong Yinghao Chu Haidong Lu Gaokuo Zhong Jie Jiang Congbing Tan Min Liao Zhihui Lu Dongwen Zhang jinbin wang Jianmin Yuan Yichun Zhou School of Materials Science and Engineering Xiangtan University411105 HunanChina Department of Physics College of ScienceNational University of Defense Technology410073 HunanChina Department of Materials Science and Engineering National Chiao Tung University30010 HsinchuTaiwan Department of Physics and Astronomy University of Nebraska-LincolnLincoln 68588USA

Domain orientations and their volume ratios in ferroelectrics are recognized as a compelling topic recently for domain switching dynamics and domain stability in devices ***,an optimized second harmonic generation method has been explored for ferroelectric domain *** a unique theoretical model with azimuth-polarization-dependent second harmonic generation response,the complex domain components and their distributions can be rigidly determined in ferroelectric thin *** the proposed model,the domain structures of rhombohedral BiFeO_(3) films with 71°and 109°domain wall,and,tetragonal BiFeO_(3),Pb(Zr_(0.2)Ti_(0.8))O_(3),and BaTiO_(3) ferroelectric thin films are analyzed and the corresponding polarization variants are *** work could provide a powerful and all-optical method to track and evaluate the evolution of ferroelectric domains in the ferroelectric-based devices.

关键词： ferroelectric harmonic second

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