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Peri-centrosomal localization of small interfering RNAs in C. elegans

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Science China Life Sciences 2025年

作者： Qile jin Xuezhu Feng Minjie Hong Ke Wang Xiangyang chen Jiewei cheng Yan Kuang Xiaoyue Si Mingjing Xu Xinya Huang Shouhong Guang chengming Zhu Department of Obstetrics and Gynecology The First Affiliated Hospital of USTC The USTC RNA Institute Hefei National Research Center for Physical Sciences at the Microscale School of Life Sciences Division of Life Sciences and Medicine Biomedical Sciences and Health Laboratory of Anhui Province University of Science and Technology of China School of Basic Medicine Anhui Medical University

The centrosome is the microtubule-organizing center and a crucial part of cell division. Centrosomal RNAs(cnRNAs) have been reported to enable precise spatiotemporal control of gene expression during cell division in many species. Whether and how cnRNAs exist in C. elegans are unclear. Here, using the nuclear RNAi Argonaute protein NRDE-3 as a reporter, we observed potential peri-centrosome localized small interfering(si)RNAs in C. elegans. NRDE-3 was previously shown to associate with pre-mRNAs and pre-rRNAs via a process involving the presence of complementary siRNAs. We generated a GFP-NRDE-3 knock-in transgene through CRISPR/Cas9 technology and observed that NRDE-3 formed peri-centrosomal foci neighboring the tubulin protein TBB-2, other centriole proteins and pericentriolar material(PCM)components in C. elegans embryos. The peri-centrosomal accumulation of NRDE-3 depends on RNA-dependent RNA polymerase(RdRP)-synthesized 22G siRNAs and the PAZ domain of NRDE-3, which is essential for siRNA binding. Mutation of eri-1, ergo-1, or drh-3 significantly increased the percentage of pericentrosome-enriched NRDE-3. At the metaphase of the cell cycle, NRDE-3 was enriched in both the peri-centrosomal region and the spindle. Moreover, the integrity of centriole proteins and pericentriolar material(PCM) components is also required for the peri-centrosomal accumulation of NRDE-3. Therefore, we concluded that siRNAs could accumulate in the pericentrosomal region in C. elegans and suggested that the peri-centrosomal region may also be a platform for RNAi-mediated gene regulation.

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A biepitope,adjuvant-free,self-assembled influenza nanovaccine provides cross-protection against H3N2 and H1N1 viruses in mice

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Nano Research 2022年第9期15卷 8304-8314页

作者： Yongbo Qiao YaXin Zhang Jie chen Shenghui jin Yarning shan National Engineering Laboratory for AIDS Vaccine School of Life SciencesJilin UniversityChangchun 130012Chin

Currently,the incorporation of multiple epitopes into vaccines is more desirable than the incorporation of a single antigen for universal influenza vaccine ***,epitopes induce poor immune *** the use of adjuvants can overcome this obstacle,it may raise new *** antigen delivery vehicles that can function as both antigen carriers and intrinsic adjuvants are highly desired for vaccine ***,we report a biepitope nanovaccine that provides complete protection in mice against H3N2 virus as well as partial protection against H1N1 *** vaccine(3MCD-f)consists of two conserved epitopes(matrix protein 2 ectodomain(M2e)and CDhelix),and these epitopes were presented on the surface of ferritin in a sequential tandem *** immunization with 3MCD-f in the absence of adjuvant induces robust humoral and cellular immune *** results provide a proof of concept for the 3MCD-f nanovaccine that might be an ideal candidate for future influenza pandemics.

关键词： influenza universal vaccine multi-epitope nanovaccine

Skeletal muscle-specific Bambi deletion induces hypertrophy and oxidative switching coupling with adipocyte thermogenesis against metabolic disorders

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Science China Life Sciences 2025年

作者： Xiangping Yao Xudong Mai Ye Tian Yifan Liu Guanghui jin Ze Li Shujie chen Xiaoshuang Dai Liujing Huang Zijing Fan Guihua Pan Xiaohan Pan Xiangmin Li Michael C Yu Jia Sun jingxing Ou Hong chen Liwei Xie College of Life and Health Sciences Guangdong Industry Polytechnic Department of Endocrinology and Metabolism Zhujiang Hospital Southern Medical University State Key Laboratory of Applied Microbiology Southern China Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application Guangdong Open Laboratory of Applied MicrobiologyInstitute of Microbiology Guangdong Academy of Sciences Institute of Aging Research Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics School of Medical Technology Guangdong Medical University Department of Hepatic Surgery and Liver transplantation Center of the Third Affiliated Hospital Organ Transplantation Institute Sun Yat-sen University Organ Transplantation Research Center of Guangdong ProvinceGuangdong province engineering laboratory for transplantation medicine Guangdong Provincial Key Laboratory of Liver Disease Research School of Public Health Xinxiang Medical University BGI Institute of Applied Agriculture BGI-Shenzhen Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University Department of Biological Sciences State University of New York at Buffalo Department of Internal Medicine Shunde Women and Children's Hospital (Maternity and Child Healthcare Hospital of Shunde Foshan) Guangdong Medical University

Skeletal muscle plays a significant role in both local and systemic energy metabolism. The current investigation aims to explore the role of the Bambi gene in skeletal muscle, focusing on its implications for muscle hypertrophy and systemic metabolism. We hypothesize that skeletal muscle-specific deletion of Bambi induces muscle hypertrophy, improves metabolic performance, and activates thermogenic adipocytes via the reprogramming of progenitor of i WAT, offering potential therapeutic strategies for metabolic syndromes. Leveraging the Chromatin immunoprecipitation(ChIP)-seq and bioinformatics analysis, Bambi gene is shown to be a direct target of HIF2α, which is further confirmed by ChIP-qPCR and promoter luciferase assay. Skeletal muscle-specific Bambi deletion led to significant muscle hypertrophy and improved metabolic parameters, even under high-fat diet conditions. This deletion induced metabolic reprogramming of stromal vascular fractions(SVFs) into thermogenic adipocytes, contributing to systemic metabolic improvements, potentially through the secretory ***, mice with skeletal muscle-specific Bambi deletion demonstrate resistance to high-fat diet-induced metabolic disorders, highlighting a potential therapeutic pathway for metabolic syndrome management. Thus, skeletal muscle-specific deletion of Bambi triggers muscle growth, enhances metabolic performance, and activates thermogenic adipocytes. These findings suggest Bambi as a novel therapeutic target for metabolic syndromes, providing new insights into the interaction between muscle hypertrophy and systemic metabolic improvement. The study underscores the potential of manipulating muscle physiology to regulate whole-body metabolism, offering a novel perspective on treating metabolic disorders.

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An AND-type 1T-FeFET array with robust write and read operations

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Science China(Information Sciences) 2025年

作者： Jiacheng XU Jiayi ZHAO Hongrui ZHANG Haoji QIAN Jiani GU Bowen chen Gaobo LIN Rongzong SHEN Xinda SONG Huan LIU Yian DING Minglei MA Miaomiao ZHANG Xiao YU Bing chen Ran chenG Gaobo XU Huaxiang YIN Yan LIU Jiajia chen chengji jin Genquan HAN Hangzhou Institute of Technology and School of Microelectronics Xidian University Zhejiang Lab College of Integrated Circuits Zhejiang University Key Laboratory of Microelectronics Devices and Integrated Technology Institute of MicroelectronicsChinese Academy of Sciences

HfO2-based ferroelectric field-effect transistor （FeF ET） has become a promising solution for next-generation embedded non-volatile memory （NVM） owing to its complementary metal-oxide-semiconductor （CMOS） compatibility,small footprint, high performance, and non-destructive readout [1]. Recent research of HfO2-based FeF ET has focused on the device operation mechanisms as well as reliability issues such as the interplay between polarization switching and charge trapping/detrapping [2], read-after-write latency [3] and endurance degradation [4]. It has been demonstrated that endurance higher than 1010cycles with negligible read-after-write latency is possible if the gate stack of FeF ET is well engineered [5].

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Metabolomic characteristics of spontaneously hypertensive rats under chronic stress and the treatment effect of Danzhi Xiaoyao Powder, a traditional Chinese medicine formula

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Journal of Integrative Medicine 2022年第1期20卷 73-82页

作者： Shuai chen jin Hu Deng-cheng Lu Hong-yi Liu shan-shan Wei College of Basic Medical Sciences Yunnan University of Chinese MedicineKunming 650500Yunnan ProvinceChina Department of Endocrinology Yunnan Provincial Hospital of Traditional Chinese MedicineKunming 650021Yunnan ProvinceChina Key Laboratory of Syndrome Micro-Differentiation of Yunnan Province Kunming 650500Yunnan ProvinceChina

Objective: Numerous studies have demonstrated the close relationship between chronic stress and blood pressure(BP). Hypertensive subjects exhibit exaggerated reactions to stress, especially higher BP. The mechanisms by which stress affects pre-existing hypertension still need to be explored. Danzhi Xiaoyao Powder(DP), a historical traditional Chinese medicine formula, is a promising treatment for BP control in hypertensive patients under stress. The present study investigated the metabolomic disruption caused by chronic stress and the treatment effect and mechanism of ***: Spontaneously hypertensive rats(SHRs) were subjected to chronic restraint stress(CRS) for 4 weeks. BP was measured via the tail-cuff method, and anxiety-like behavior was quantified using the elevated-plus-maze test. Meanwhile, DP was administered intragastrically, and its effects were observed. Global metabolomic analysis was performed using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, followed by multivariate statistical analysis to detect differential metabolites and ***: DP alleviated the CRS-induced increase in BP and anxiety-like behavior. Systematic metabolic differences were found among the three study groups. A total of 29 differential plasma metabolites were identified in both positive-and negative-ion modes. These metabolites were involved in triglyceride metabolism, amino acid(phenylalanine, tryptophan, and glycine) metabolism, and steroid hormone ***: These findings expose the metabolomic disturbances induced by chronic stress in SHRs and suggest an innovative treatment for this disorder.

关键词： Hypertension Stress Metabolomics Danzhi Xiaoyao Powder

不同海拔高度下电晕放电等离子体粒子特性的仿真研究

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原子与分子物理学报 2025年第3期42卷 94-100页

作者：冷雪健金福宝马山刚陈科元青海大学能源与电气工程学院西宁810000

电晕放电作为等离子体的一种重要获取手段,它的放电特性及其降解能力与所处空气环境有着密切的关系.为了得到不同海拔高度对电晕放电特性和等离子体降解能力的影响,本文通过COMSOL软件基于流体动力学模型,对不同海拔高度的放电模型进行... 详细信息

电晕放电作为等离子体的一种重要获取手段,它的放电特性及其降解能力与所处空气环境有着密切的关系.为了得到不同海拔高度对电晕放电特性和等离子体降解能力的影响,本文通过COMSOL软件基于流体动力学模型,对不同海拔高度的放电模型进行数值模拟计算.分别对电流波形、影响等离子体降解能力的电子密度、平均电子能量等物理量随海拔高度变化的规律进行研究.结果表明,海拔越高,气压越低,环境温度越低,电流脉冲峰值越高,频率越高,脉冲启动时间越提前.脉冲波形的上升时间减小、下降时间增大.同时,电子密度以及平均电子能量也会随着海拔高度的升高而增强.

关键词：等离子体电晕放电 COMSOL仿真电子密度

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Density Functional Theory Study of Water Diffusion and Clustering on Pd(111)

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Chinese Journal of Structural Chemistry 2006年第8期25卷 909-914页

作者： chen jin-Wen TU Xue-Yan TIAN Kai DAI Shu-shan School of Chemical Science and Technology Yunnan University Kunming 650091 China School of Chemistry. and Biotechnology Yunnan Nationalities University Kunming 650031 China School of Chemistry and Chemical Engineering Hunan Universi of Science and Technology Xiangtan 411201 China

The internal structures as well as adsorption and hopping energies of monomers, dimers, trimers, tetramers, pentamers and hexamers of water on Pd（111） have been studied by density functional theory （DFT） plane-wave pseudopotential method which performs the firstprinciples quantum-mechanical calculations to explore the properties of crystals and surfaces in materials. Based on the calculations, we suppose that their absorption is via one water molecule for monomers, dimmers and trimers, but three water molecules for pentamers and hexamers. Moreover, there is one water molecule bonding with Pd atom by O atom in pentamers and hexamers, which explains why pentamers and hexamers are stable. The binding energies of polymers may be used to explain why the trimer comes close to two nearby monomers to form a stable pentamer instead of tetramer. And the difference of mobility of small water clusters is due to their different hopping energies.

关键词： density functional theory Pd(111) surface water diffusion and clustering binding energy hopping energy

A Corrected Solid Boundary Treatment Method for Smoothed Particle Hydrodynamics

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China Ocean Engineering 2017年第2期31卷 238-247页

作者： chen Yun-sai ZHENG Xing jin shan-qin DUAN Wen-yang College of Shipbuilding Engineering Harbin Engineering University Harbin 150001 China Department of Technology National Deep Sea Center Qingdao 266061 China

Smoothed Particle Hydrodynamics method （SPFI） has a good adaptability for simulating of free surface flow problems. However, there are some shortcomings of SPH which are still in open discussion. This paper presents a corrected solid boundary handling method for weakly compressible SPH. This improved method is very helpful for numerical stability and pressure distribution. Compared with other solid boundary handling methods, this corrected method is simpler for virtual ghost particle interpolation and the ghost particle evaluation relationship is clearer. Several numerical tests are given, like dam breaking, solitary wave impact and sloshing tank waves. The results show that the corrected solid boundary processing method can recover the spurious oscillations of pressure distribution when simulating the problems with complex geometry boundary.

关键词： SPH particle arrangement boundary treatment solitary wave impact sloshing wave

Superconducting Magnetic Energy Storage Integrated Current-source DC/DC Converter for Voltage Stabilization and Power Regulation in DFIG-based DC Power Systems

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Journal of Modern Power Systems and Clean Energy 2023年第4期11卷 1356-1369页

作者： Ruohuan Yang Jianxun jin Qian Zhou Mingshun Zhang shan Jiang Xiaoyuan chen School of Electrical and Information Engineering Tianjin UniversityTianjin 300072China School of Engineering Sichuan Normal UniversityChengdu 610101China

Unpredictable power fluctuation and fault ridethrough capability attract increased attention as two uncertain major factors in doubly-fed induction generators(DFIGs)integrated DC power *** solutions usually require complicated cooperation comprising multiple modules of energy storage,current control,and voltage *** overcome the drawbacks of existing solutions,this paper proposes a superconducting magnetic energy storage(SMES)integrated currentsource DC/DC converter(CSDC).It is mainly composed of a current-source back-to-back converter,and the SMES is tactfully embedded in series with the intermediate DC *** proposed SMES-CSDC is installed in front of the DC-DFIG to carry out its dual abilities of load voltage stabilization under multifarious transient disturbances and power regulation under wind speed *** with the existing DC protection devices,the SMES-CSDC is designed on the basis of unique current-type energy *** has the advantages of fast response,extensive compensation range,concise hardware structure,and straightforward control *** feasibility of the SMESCSDC is implemented via a scaled-down experiment,and its effectiveness for DC-DFIG protection is verified by a large-scale DC power system simulation.

关键词： Superconducting magnetic energy storage(SMES) DC doubly-fed induction generator(DC-DFIG) current-source converter voltage stabilization power regulation