检索结果-南通市图书馆

Patient-derived induced pluripotent stem cells with a MERTK mutation exhibit cell junction abnormalities and aberrant cellular differentiation potential

引用

World Journal of Stem Cells 2024年第5期16卷 512-524页

作者： Hang Zhang Ling-Zi wu Zhen-Yu Liu Zi-Bing jin Beijing Institute of Ophthalmology Beijing Tongren Eye CenterBeijing Tongren HospitalCapital Medical UniversityBeijing 100730China

BACKGROUND Human induced pluripotent stem cell(hiPSC)technology is a valuable tool for generating patient-specific stem cells,facilitating disease modeling,and invest-igating disease ***,iPSCs carrying specific mutations may limit their clinical applications due to certain inherent *** To investigate the impact of MERTK mutations on hiPSCs and determine whether hiPSC-derived extracellular vesicles(EVs)influence anomalous cell junction and differentiation *** We employed a non-integrating reprogramming technique to generate peripheral blood-derived hiPSCs with and hiPSCs without a MERTK ***-somal karyotype analysis,flow cytometry,and immunofluorescent staining were utilized for hiPSC *** and proteomics were employed to elucidate the expression patterns associated with cell junction abnormalities and cellular differentiation ***,EVs were isolated from the supernatant,and their RNA and protein cargos were examined to investigate the involvement of hiPSC-derived EVs in stem cell junction and *** The generated hiPSCs,both with and without a MERTK mutation,exhibited normal karyotype and expressed pluripotency markers;however,hiPSCs with a MERTK mutation demonstrated anomalous adhesion capability and differentiation potential,as confirmed by transcriptomic and proteomic ***,hiPSC-derived EVs were involved in various biological processes,including cell junction and *** HiPSCs with a MERTK mutation displayed altered junction characteristics and aberrant differentiation ***,hiPSC-derived EVs played a regulatory role in various biological processes,including cell junction and differentiation.

关键词： Cell junction Cellular differentiation Extracellular vesicle Human induced pluripotent stem cells Transcriptomics Proteomics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Network Pharmacology Based Elucidation of Molecular Mechanisms of Laoke Formula for Treatment of Advanced Non-Small Cell Lung Cancer

引用

Chinese Journal of Integrative Medicine 2024年第11期30卷 984-992页

作者： FENG Yu-yu LIU jin-feng XUE Yang LIU Dan wu Xiong-zhi Department of Nursing Tangshan Vocational and Technical College School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine Department of Oncology Tianjin Medical University General Hospital Tianjin Medical University Cancer Institute and Hospital National Clinical Research Center for Cancer Key Laboratory of Cancer Prevention and Therapy Tianjin's Clinical Research Center for China Tianjin Nankai Hospital Tianjin Medical University

Objective: To explore the specific pharmacological molecular mechanisms of Laoke Formula(LK)on treating advanced non-small cell lung cancer(NSCLC) based on clinical application, network pharmacology and experimental validation. Methods: Kaplan-Meier method and Cox regression analysis were used to evaluate the survival benefit of Chinese medicine(CM) treatment in 296 patients with NSCLC in Tianjin Medical University Cancer Institute and Hospital from January 2011 to December 2015. The compounds of LK were screened using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, and the corresponding targets were performed from Swiss Target Prediction. NSCLC-related targets were obtained from Therapeutic Target Database and Comparative Toxicogenomics Database. Key compounds and targets were identified from the compound-target-disease network and protein-protein interaction(PPI) network analysis, respectively. Kyoto Encyclopedia of Genes and Genomes(KEGG) and Gene Ontology(GO) enrichment analysis were used to predict the potential signaling pathways involved in the treatment of advanced NSCLC with LK. The binding affinities between key ingredients and targets were further verified using molecular docking. Finally, A549 cell proliferation and migration assay were used to evaluate the antitumor activity of LK. Western blot was used to further verify the expression of key target proteins related to the predicted pathways. Results: Kaplan-Meier survival analysis showed that the overall survival of the CM group was longer than that of the non-CM group(36 months vs.26 months), and COX regression analysis showed that LK treatment was an independent favorable prognostic factor(P=0.027). Next, 97 components and 86 potential targets were included in the network pharmacology, KEGG and GO analyses, and the results indicated that LK was associated with proliferation and apoptosis. Moreover,molecular docking revealed a good binding affinity between the key

关键词： Chinese medicine LaoKe Formula anti-tumor network pharmacology non-small cell lung cancer

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Azobenzene-based ultrathin peptoid nanoribbons for the potential on highly efficient artificial light-harvesting

引用

Science China Chemistry 2024年第5期67卷 1680-1686页

作者： Zichao Sun Lingrong Zhu jingze Liu Fan Liu Pengchao wu Pengliang Sui Haibao jin Shaoliang Lin Shanghai Key Laboratory of Advance Polymeric Materials Frontiers Science Center for Materiobiology and Dynamic ChemistrySchool of Materials Science and EngineeringEast China University of Science and TechnologyShanghai 200237China

The development of artificial light-harvesting systems based on long-range ordered ultrathin organic nanomaterials(i.e., below3 nm), which were assembled from stimuli-responsive sequence-controlled biomimetic polymers, remains challenging. Herein,we report the self-assembly of azobenzene-containing amphiphilic ternary alternating peptoids to construct photo-responsive ultrathin peptoids nanoribbons(UTPNRs) with a thickness of ~2.3 nm and the length in several micrometers. The pendants hydrophobic conjugate stacking mechanism explained the formation of one-dimensional ultrathin nanostructures, whose thickness was highly dependent on the length of side groups. The photo-isomerization of azobenzene moiety endowed the aggregates with a reversible morphology transformation from UTPNRs to spherical micelles(46.5 nm), upon the alternative irradiation with ultraviolet and visible light. Donor of 4-(2-hydroxyethylamino)-7-nitro-2,1,3-benzoxadiazole(NBD) and acceptor of rhodamine B(RB) were introduced onto the hydrophobic and hydrophilic regions, respectively, to generate photocontrollable artificial light-harvesting systems. Compared with the spheres-based systems, the obtained NBD-UTPNRs@RB composite proved a higher energy transfer efficiency(90.6%) and a lower requirement of RB acceptors in water. A proof-ofconcept use as fluorescent writable ink demonstrated the potential of UTPNRs on information encryption.

关键词： azobenzene-based peptoid ultrathin nanoribbon photo-responsive light-harvesting information encryption

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Boosting thermal stability and crystallization of closed-loop-recyclable biodegradable poly(p-dioxanone) by end-group regulation

引用

Science China Chemistry 2024年第2期67卷 642-651页

作者： jin Lei Zhu-Xin Gui Wan-Ting Xiong Gang wu Si-Chong Chen Yu-Zhong Wang The Collaborative Innovation Center for Eco-Friendly and Fire-Safety Polymeric Materials(MoE) National Engineering Laboratory of Eco-Friendly Polymeric Materials(Sichuan)State Key Laboratory of Polymer Materials EngineeringCollege of ChemistrySichuan UniversityChengdu610064China

A chemically closed-loop-recyclable biodegradable polymer,poly(p-dioxanone)(PPDO),is one of the ideal candidates for single-use plastic products due to its suitability for different application ***,when PPDO wastes can be collected,its monomer p-dioxanone(PDO) will be obtained through chemical recycling of these wastes;when cannot be collected,the wastes are able to be biodegraded into harmless ***,unsatisfied thermal stability and low crystallization rate of PPDO restrict its wider ***,based on end-group regulation,we simultaneously realized the significant enhancement of thermal stability and crystallization of PPDO through the simple melt processing with tributyl phosphite(TBP) or triphenyl phosphite(TPP).The model reactions were conducted to investigate the reaction mechanism and theoretical products during the preparation of PPDO/phosphite *** kinds of phosphites were proved to act as the end-capped reagent and chain extender in the melt processing,while TBP presented better *** a result,the activation energy of thermal decomposition was largely elevated,and the unprecedented T_(5%)(the temperature at a weight loss of 5%) and Tmax(the temperature at a maximum rate of weight loss) of PPDO were obtained,i.e.,T_(5%)of ~330 ℃ and Tmaxof ~385 ℃ in N_(2) atmosphere,T_(5%)of ~240 ℃ and Tmaxof ~317 ℃ in air atmosphere,***,the increased crystallization rate,crystallinity,crystalline orderliness,and realizable monomer recovery(yield >90%,purity >99.9%) of PPDO/phosphite compounds were confirmed.

关键词： poly(p-dioxanone) phosphite thermal stability crystallization end-group regulation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

CAEFusion: A New Convolutional Autoencoder-Based Infrared and Visible Light Image Fusion Algorithm

引用

Computers, Materials & Continua 2024年第8期80卷 2857-2872页

作者： Chun-Ming wu Mei-Ling Ren jin Lei Zi-Mu Jiang Key Laboratory of Modern Power System Simulation and Control&Renewable Energy Technology Ministry of EducationSchool of Electrical EngineeringNortheast Electric Power UniversityJilin132012China School of Electrical Engineering Northeast Electric Power UniversityJilin132012China School of Electronic Information Engineering Bozhou UniversityBozhou236800China

To address the issues of incomplete information,blurred details,loss of details,and insufficient contrast in infrared and visible image fusion,an image fusion algorithm based on a convolutional autoencoder is *** region attention module is meant to extract the background feature map based on the distinct properties of the background feature map and the detail feature map.A multi-scale convolution attention module is suggested to enhance the communication of feature *** the same time,the feature transformation module is introduced to learn more robust feature representations,aiming to preserve the integrity of image *** study uses three available datasets from TNO,FLIR,and NIR to perform thorough quantitative and qualitative trials with five additional *** methods are assessed based on four indicators:information entropy(EN),standard deviation(SD),spatial frequency(SF),and average gradient(AG).Object detection experiments were done on the M3FD dataset to further verify the algorithm’s performance in comparison with five other *** algorithm’s accuracy was evaluated using the mean average precision at a threshold of 0.5(mAP@0.5)*** experimental findings show that CAEFusion performs well in subjective visual and objective evaluation criteria and has promising potential in downstream object detection tasks.

关键词： Image fusion deep learning auto-encoder(AE) infrared visible light

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Predictive value of neutrophil-to-lymphocyte ratio in coronary chronic total occlusion patients

引用

Journal of Geriatric Cardiology 2024年第5期21卷 542-549页

作者： Qian LI Yue YU Ya-Qiong ZHOU Yi ZHAO jin wu Yuan-jing wu Bin DU Pei-Jian WANG Tao ZHENG Department of Cardiovascular Medicine the First Affiliated Hospital of Xi’an Jiaotong UniversityXi’anChina Department of Cardiology First Affiliated Hospital of Chengdu Medical CollegeChengduChina Department of Neurology the First Affiliated Hospital of Xi’an Jiaotong UniversityXi’anChina

BACKGROUND The neutrophil to lymphocyte ratio(NLR)has been reported as a novel predictor for atherosclerosis and car-diovascular *** study aimed to determine the effects of NLR on long-term clinical outcomes of chronic total occlusion(CTO)*** A total of 670 patients with CTO who met the inclusion criteria were included at the end of the follow-up *** were divided into tertiles according to their baseline NLR levels at admission:low(n=223),intermediate(n=223),and high(n=224).The incidence of major adverse cardiac events(MACEs)during the follow-up period,including all-cause death,nonfatal myocardial infarction(MI),or ischemia-driven revascularization,were compared among the three *** Major adverse cardiac events were observed in 27 patients(12.1%)in the low tertile,40(17.9%)in the intermediate tertile,and 61(27.2%)in the high NLR tertile(P<0.001).Kaplan-Meier analysis demonstrated a significantly higher incidence of MACE,ischemia-driven coronary revascularization,non-fatal MI,and mortality in patients within the high tertile than those in the low and intermediate groups(all P<0.001).Multivariable COX regression analysis showed that the high tertile of baseline NLR level showed a strong association with the risk of MACE(hazard ratio[HR]=2.21;95%confidence interval[CI]:1.21-4.03;P=0.009),ischemia-driven coronary revascularization(HR=3.19;95%CI:1.56-6.52;P=0.001),MI(HR=2.61;95%CI:1.35-5.03;P=0.043)and mortality(HR=3.78;95%CI:1.65-8.77;P=0.001).CONCLUSION Our findings suggest that NLR is an inexpensive and readily available biomarker that can independently pre-dict cardiovascular risk in patients with CTO.

关键词： patients coronary occlusion

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Enhancing the Circularly Polarized Luminescence of Self-assembled Cyanostilbenes through Extended π-Conjugation

引用

Chinese Journal of Chemistry 2024年第2期42卷 135-141页

作者： Tongjin Lv Song wu Yu jin Jianfei Ma Sixun Jiang Yuncong Xue Feng Wang Key Laboratory of Precision and Intelligent Chemistry Department of Polymer Science and EngineeringUniversity of Science and Technology of ChinaHefeiAnhui 230026China Department of Thoracic Surgery Shanghai Jiao Tong University Affiliated Sixth People's HospitalShanghai 200233China

Chiral supramolecular assembly of π-conjugated luminophores provides a promising avenue for enhancing circularly polarized *** this study,we shed light on the impact of π-conjugation length on circularly polarized luminescent performance of the resulting supramolecular assemblies,by designing a tetra-cyanostilbene monomeric compound alongside two dicyanostilbene control *** cyanostilbene-based compounds possess the ability to form chiral supramolecular polymers in toluene,driven by a synergistic combination of intermolecular hydrogen bonding and π-stacking *** extended π π-aromatic skeleton brings bathochromic-shifted fluorescence and enhanced intermolecular stacking capability for the tetra-cyanostilbene ***,chiral supramolecular assemblies formed by the tetra-cyanostilbene compound demonstrate a remarkable two-fold increase in g_(lum) values relative to the assemblies formed by the dicyanostilbene ***,this study provides valuable insights into the relationship between-conjugation length and the circularly polarized luminescent performance of π-conjugatedsupramolecularassemblies.

关键词： Self-assembly Supramolecular polymer Supramolecular chirality Circularly polarized luminescence Cyanostilbene πInteractions Aggregation Nanostructures

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Targeting the Warburg effect:A revisited perspective from molecular mechanisms to traditional and innovative therapeutic strategies in cancer

引用

Acta Pharmaceutica Sinica B 2024年第3期14卷 953-1008页

作者： Minru Liao Dahong Yao Lifeng wu Chaodan Luo Zhiwen Wang jin Zhang Bo Liu Department of Biotherapy Cancer Center and State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China School of Pharmaceutical Sciences Shenzhen Technology UniversityShenzhen 518118China School of Pharmacy Shenzhen University Medical SchoolShenzhen UniversityShenzhen 518055China Department of Psychology University of Southern CaliforniaLos AngelesCA 90089USA

Cancer reprogramming is an important facilitator of cancer development and survival,with tumor cells exhibiting a preference for aerobic glycolysis beyond oxidative phosphorylation,even under sufficient oxygen supply *** metabolic alteration,known as the Warburg effect,serves as a significant indicator of malignant tumor *** Warburg effect primarily impacts cancer occurrence by influencing the aerobic glycolysis pathway in cancer *** enzymes involved in this process include glucose transporters(GLUTs),HKs,PFKs,LDHs,and ***,the expression of transcriptional regulatory factors and proteins,such as FOXM1,p53,NF-κB,HIF1a,and c-Myc,can also influence cancer ***,lncRNAs,miRNAs,and circular RNAs play a vital role in directly regulating the Warburg ***,gene mutations,tumor microenvironment remodeling,and immune system interactions are closely associated with the Warburg ***,the development of drugs targeting the Warburg effect has exhibited promising potential in tumor *** comprehensive review presents novel directions and approaches for the early diagnosis and treatment of cancer patients by conducting in-depth research and summarizing the bright prospects of targeting the Warburg effect in cancer.

关键词： Warburg effect Cancer Regulatory factor LncRNAs miRNAs Traditional Chinese medicine Small-molecule drug Drug resistance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Changes of nutrients and microbial communities in recovery process of abandoned rare earth tailings

引用

Pedosphere 2024年第4期34卷 826-836页

作者： Bing ZHANG Junli wu Mengyuan HUANG Yan ZHANG jin ZHAO Chuntao HE Zhongyi YANG School of Environmental Science and Engineering Sun Yat-sen UniversityGuangzhou 510006China School of Life Sciences Sun Yat-sen UniversityGuangzhou 510275China School of Agriculture State Key Laboratory for BiocontrolShenzhen Campus of Sun Yat-sen UniversitySun Yat-sen UniversityShenzhen 518107China

Rare earth element(REE)mining has wrought severe ecosystem destruction,particularly in the loss of carbon(C),nitrogen(N),and phosphorus(P).Furthermore,its effects on nutrient recovery characteristics and associated microbial abundance are still poorly *** this study,an investigation was conducted to determine C,N,and P recovery patterns and microbial communities in abandoned REE mining tailings with different abandoned durations of 1.5(1.5-Y),7(7-Y),10(10-Y),and 14(14-Y)years and at an unmined site(the control group)in Jiangxi Province,*** tailing delamination at each site was investigated in 0–15(layer 1)and 15–30(layer 2)cm tailing *** the abandoned duration of REE tailings,C and P in layer 1 continued to recover from 1.62 to 8.51 g kg^(-1) and from 0.71 to 1.94 g kg^(-1),respectively;N losses of 0.60 and 0.22 g kg^(-1) occurred at the 1.5-Y and 10-Y sites,while N in layer 1 slightly increased at the 14-Y ***,Methylosinus,Bradyrhizobium,Pseudomonas,and Azospirillum were dominant bacteria at the 14-Y site and in the *** relative abundances of N-fixing bacteria Mesorhizobium(32.94%),Sinorhizobium(0.24%),Frankia(0.71%),and Burkholderia(2.38%)at the 14-Y site were 1.33–3.97 times those in the control(24.19%,0.18%,0.32%and 0.60%,respectively),which was helpful for tailing N *** the 14-Y site,Tylospora(50.24%),Luellia(11.02%),Tomentella(6.94%),and Chaetomium(4.34%)were the dominant fungal genera,while Tylospora(56.93%),Suillus(11.45%),and Penicillium(6.78%)predominated in the *** relative abundance of the P-dissolving fungus Aspergillus at the 14-Y site(0.42%)was 4.2 times that in the control(0.1%),which may lead to the improved tailing P *** the 14-Y site,woody plants such as Pinus massoniana and native pioneering herbs and ferns such as Dicranopteris dichotoma and Nephrolepis auriculata probably invaded from adjacent *** study unraveled the natural restoration of plant and micro

关键词： abandoned duration mining tailing N-fixing bacteria nutrient recovery P-solubilizing fungi rare earth element

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Role of Renewable Protocatechol Acid in Epoxy Coating Modification: Significantly Improved Antibacterial and Adhesive Properties

引用

Chinese Journal of Polymer Science 2024年第1期42卷 63-72,I0008页

作者： Ming-Xuan Chen jin-Yue Dai Li-Yue Zhang Shuai-Peng Wang jing-Kai Liu Yong-Gang wu Xin-wu Ba Xiao-Qing Liu School of Chemistry and Materials Science Hebei UniversityBaoding 071000China Ningbo Institute of Materials Technology and Engineering Chinese Academy of SciencesNingbo 315201China Key Laboratory of Marine Materials and Related Technologies Chinese Academy of SciencesNingbo 315201China

It is of great significance to design epoxy coatings with superior antibacterial properties and high adhesive properties, as well as excellent processing, superior durability, and high transparency. However, it is still a challenge because of the common complex design and synthesis. Herein, the bio-based monomer protocatechuic acid(PCA) was used as raw material, the catechol structure with high bonding and antibacterial properties was introduced into the flexible alkane segment of ethylene glycol diglycidyl ether(EGDE) through an efficient, and green method, and it was cured with isophorone diamine(IPDA) to prepare corresponding thermosets. The cured resins exhibited excellent allaround qualities, particularly in bonding and antibacterial. When 30% PCA was added to pure epoxy resin, the adhesion between substrate and coating increased from 4.40 MPa to 13.60 MPa and the antibacterial rate of coating against E. coli and S. aureus could approach 100%. All of this is due to the fact that the catechol structure present in PCA has the ability to interact with various substrates and alter the permeability of bacterial cell membranes. The architecture of this method offers a fresh approach to dealing with the issues of challenging raw material selection and complex synthesis techniques.

关键词： Epoxy coatings Thermosets Catechol structure Antibacterial High adhesion

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：