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Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems

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Zoological Research 2016年第4期37卷 205-213页

作者： Li-Fang jin jin-song LI College of Life Science of Shaoxing University Shaoxing Zhejiang 312000 China State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell Science Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Science Shanghai 200031 China Shanghai Key Laboratory of Molecular Andrology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological SciencesChinese Academy of Science Shanghai 200031 China

With the development of high-throughput sequencing technology in the post-genomic era, researchers have concentrated their efforts on elucidating the relationships between genes and their corresponding functions. Recently, important progress has been achieved in the generation of genetically modified mice based on CRISPR/Cas9 and haploid embryonic stem cell （haESC） approaches, which provide new platforms for gene function analysis, human disease modeling, and gene therapy. Here, we review the CRISPR/Cas9 and haESC technology for the generation of genetically modified mice and discuss the key challenges in the application of these approaches.

关键词： CRISPR/Cas9 Haploid embryonic stem cells Mouse Genetic modification

Rapid Absolute Determination Platform of Nucleic Acid for Point-of-care Testing

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Chemical Research in Chinese Universities 2015年第4期31卷 519-525页

作者： song Bofan jin Wei song Qi jin Qinhan MU Ying Research Center for Analytical Instrumentation State Key Laboratory of lndustrial Control Technology Zhejiang University Hangzhou 310058 P. R. China

Nowadays nucleic acid tests are promising to be considered as point-of-care testing（POCT）. However, no such devices are currently available that can perform all the functions, including absolute nucleic acid determination, worldwide on-site detection, rapid analysis and real-time results reporting via ubiquitous mobile networks simultaneously with full package and automated means of measurement. In this study, we presented a compact low-cost portable POC automated testing platform with all attributes mentioned above. A disposable self-priming compartmentalization（SPC） microfluidic chip is used to conduct isothermal amplification. The platform also includes a micro-computer controlled heating unit, an inexpensive optical imaging setup, and a mobile device with customized software. It may become a useful tool for the rapid on-site detection of infectious diseases as well as other pathogens.

关键词： Point-of-care testing Microfluidic chip Loop-mediated isothermal amplification Mobile medicine

Mechanisms of cadmium phytoremediation and detoxification in plants

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The Crop Journal 2021年第3期9卷 521-529页

作者： jin-song Luo Zhenhua Zhang College of Resources and Environmental Sciences Hunan Agricultural UniversityChangsha 410128HunanChina Hunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use Hunan Provincial Key Laboratory of Nutrition in Common UniversityNational Engineering Laboratory on Soil and Fertilizer Resources Efficient UtilizationChangsha 410128HunanChina

As a consequence of industrial development,soil Cd pollution leads to crop contamination by Cd,posing a threat to food safety and human *** accumulation of Cd in plants also inhibits their growth via oxidative stress damage to their photosynthetic *** evolutionary selection,plants have developed a set of efficient strategies to respond to Cd in their *** include the accumulation and detoxification of heavy *** is absorbed by plant roots through the apoplastic and symplastic pathways and then translocated to plant shoots via xylem loading,long-distance transport,and phloem ***,plants initiate a series of mechanisms to reduce Cd toxicity,including cell wall adsorption,cytoplasmic chelation,and vacuolar *** review summarizes current knowledge of Cd accumulation and detoxification in plants.

关键词： Uptake Translocation Vacuolar sequestration Chelation

NUMERICAL STUDIES OF INTERNAL SOLITARY WAVE GENERATIONAND EVOLUTION BY GRAVITY COLLAPSE

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Journal of Hydrodynamics 2012年第4期24卷 541-553页

作者： LIN Zhen-hua song jin-bao Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Key Laboratory of Ocean Circulation and Waves Chinese Academy of Sciences Qingdao 266071 China

In this study, an analysis on the internal wave generation via the gravity collapse mechanism is carried out based on the theoretical formulation and the numerical simulation. With the linear theoretical model, a rectangle shape wave is generated and propagates back and forth in the domain, while a two-dimensional non-hydrostatic numerical model could reproduce all the observed phenomena in the laboratory experiments conducted by Chen et al. （2007）, and the related process realistically. The model results further provide more quantitative information in the whole domain, thus allowing an in depth understanding of the corresponding internal solitary wave generation and propagation. It is shown that the initial type of the internal wave is determined by the relative height between the perturbation and the environmental density interface, while the final wave type is related to the relative height of the upper and lower layers of the environmental fluid. The shape of the internal wave generated is consistent with that predicted by the KdV and EKdV theories if its amplitude is small, as the amplitude becomes larger, the performance of the EKdV becomes better after the wave adjusts itself to the ambient stratification and reaches an equilibrium state between the nonlinear and dispersion effects. The evolution of the mechanical energy is also analyzed.

关键词： gravity collapse intemal wave energy analysis

Dominance property based tabu search for single machine scheduling problems with family setups

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Journal of Systems Engineering and Electronics 2009年第6期20卷 1233-1238页

作者： jin Feng song Shiji Wu Cheng Dept. of Automation Tsinghua Univ. Beijing 100084 P. R. China

The problem of minimizing the maximum lateness on a single machine with family setups is *** solve the problem, dominance property is studied and then introduced into the tabu search（TS） *** the dominance property, most unpromising neighbors can be excluded from the neighborhood, which makes the search process always focus on the most promising areas of the solution *** proposed algorithms are tested both on the randomly generated problems and on the real-life *** results show that the proposed TS algorithm outperforms the best existing algorithm and can solve the real-life problems in about 1.3 on average.

关键词： single machine family setups tabu search dominance property.

NS codoped carbon nanorods as anode materials for high-performance lithium and sodium ion batteries

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Journal of Energy Chemistry 2018年第1期27卷 203-208页

作者： Ajuan Hu song jin Zhenzhen Du Hongchang jin Hengxing Ji Department of Materials Science and Engineering CAS Key Laboratory of Materials for Energy Conversion iChEM University of Science and Technology of China

NS codoped carbon nanorods（NS-CNRs） were prepared using crab shell as template and polyphenylene sulfide（PPS） as both the C and S precursor, followed by carbonization in NH_3. The as-obtained NS-CNRs had a diameter of ~50 nm, length of several micrometers, and N and S contents of 12.5 at.% and 3.7 at.%,respectively, which can serve as anodes for both lithium-ion batteries（LIBs） and sodium ion batteries（SIBs）. When serving as an anode of LIB, the NS-CNRs delivered gravimetric capacities of 2154 mAh g^（-1）at current densities of 0.1 A g^（-1）and 625 mAh g^（-1）at current densities of 5.0 A g^（-1）for 1000 *** serving as an anode of SIB, the NS-CNRs delivered gravimetric capacities of 303 mAh g^（-1）at current densities of 0.1 A g^（-1）and 230 mAh g^（-1）at current densities of 1.0 A g^（-1）for 3000 cycles. The excellent electrochemical performance of NS-CNRs could be ascribed to the one-dimensional nanometer structure and high level of heteroatom doping. We expect that the obtained NS-CNRs would benefit for the future development of the doped carbon materials for lithium ion batteries and other extended applications such as supercapacitor, catalyst and hydrogen storage.

关键词： NS codoped carbon Crab shell template Lithium ion batteries Sodium ion batteries

Effect of nano-carbon black content on wetting phenomenon of molten steel and alumina-carbon ceramic filter substrates

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Journal of Iron and Steel Research International 2024年第8期31卷 1900-1913页

作者： jin-wen song Wen Yan Zhe Chen Ying Liu Shao-song Hong The State Key Laboratory of Refractories and Metallurgy Wuhan University of Science and TechnologyWuhan 430081HubeiChina National-provincial Joint Engineering Research Center of High Temperature Materials and Lining Technology Wuhan University of Science and TechnologyWuhan 430081HubeiChina Jiangsu Sheng Nai New Materials Co. Ltd.Taizhou 225503JiangsuChina

The effect of nano-carbon black content(O,8 and 12 wt.%)on the wettability of molten steel on Al_(2)O_(3)-C substrates was investigated by the sessile drop wetting method at 1500℃ under argon *** the beginning of the wetting experiment,the contact angle decreased with the increase in nano-carbon black *** the wetting experiment progressed,FeAl_(2)0_(4) layer and sheet Al_(2)O_(3) layer were found at the interface between the molten steel and the Al_(2)O_(3)-C substrates with O and 8 wt.% nano-carbon black content,and the contact angle deceased with *** the content of nano-carbon black was 12 wt.%,a large number of nano-Al_(2)O_(3) whiskers were observed,which made the contact angle between the molten steel and Al_(2)O_(3)-C substrate become *** on the scanning electron microscope and energy dispersive spectrometry results,the formation mechanism of FeAl2O4 layer and Al_(2)O_(3) layer and the interfacial reaction mechanism were proposed.

关键词： Contact angle Nano-carbon black Al_(2)O_(3)-C substrate Molten steel Interfacial reaction

Comparative ultrastructural study of endoplasmic reticulum in colorectal carcinoma cell lines with different degrees of differentiation

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World Journal of Gastroenterology 2000年第4期6卷 569-571页

作者： Shu Feng jin Dan song Microbial Engineering Department Institute of Applied EcologyChinese Academy of SciencesShenyang 110015Liaoning ProvinceChina Key Laboratory of Cell Biology Ministry of Public Health of ChinaChina Medical UniversityShenyang 110001Liaoning ProvinceChina

The endoplasmic reticulum (ER) consists of a complex system of tubules, lamellae, and flattened vesicles, and has a variety of morphologies in different cells. It is believed to play a central role in the biosynthesis of cholesterol, phospholipids, steroids, prostaglandins, membrane and secretory proteins[1]. Cancer cells have different functions and ultrastmcture from their original cells[2-4]. The studies on ER membrane system of cancer cells are of great significance in understanding their malignant behavior. In the present work, the ultrastructural characteristics of ER in human colorectal carcinoma cell lines with different differentiation degrees were investigated.

关键词： colorectal neoplasms endoplasmic reticulum ultrastructure microscopy electron microscopy electron scanning cell differentiation

The atom-bond connectivity index of chemical bicyclic graphs

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Applied Mathematics(A Journal of Chinese Universities) 2012年第2期27卷 243-252页

作者： CHEN jin-song GUO Xiao-feng College of Mathematics and Computer Science Hunan Normal University Changsha 410081 China. College of Mathematics and Computer Science Fuzhou University Fuzhou 350108 China. School of Mathematical Sciences Xiamen University Xiamen 361005 China.

The atom-bond connectivity（ABC） index provides a good model for the stability of linear and branched alkanes as well as the strain energy of cycloalkanes,which is defined as ABC（G） =∑ uv∈E（G） √d u＋dv-2 dudv,where du denotes the degree of a vertex u in G.A chemical graph is a graph in which no vertex has degree greater than *** this paper,we obtain the sharp upper and lower bounds on ABC index of chemical bicyclic graphs.

关键词： atom-bond connectivity index chemical bicyclic graph bound.