检索结果-南通市图书馆

Intestinal failure:Pathophysiological elements and clinical diseases

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2004年第7期10卷 930-933页

作者： Lian-AnDing jie-shouli DepartmentofGeneralSurgery AffiliatedHospitalofQingdaoUniversityMedicalSchool16JiangsuRoadQingdao266003ShandongProvinceChina ResearchInstituteofGeneralSurgery ClinicalCollegeofNanjingUniversityMedicalSchool305EastZhongshanRoadNanjing210002JiangsuProvinceChina

There are two main functions of gastrointestinal tract, digestion and absorption, and barrier function. The latter has an important defensive effect, which keeps the body away from the invading and damaging of bacteria and endotoxin. It maintains the systemic homeostasis. Intestinal dysfunction would happen when body suffers from diseases or harmful stimulations. The lesser dysfunction of GI tract manifests only disorder of digestion and absorption, whereas the more serious intestinal disorders would harm the intestinal protective mechanism, or intestinal barrier function, and bacterial/endotoxin translocation, of intestinal failure (IF) would ensue. This review disscussed the theory of the intestinal failure, aiming at attracting recognition and valuable comments by clinicians.

关键词：肠道功能障碍病理生理学临床特点消化功能免疫功能

Modified techniques of heterotopic total small intestina transplantation in rats

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2002年第4期8卷 758-762页

作者： Xiao-TingWu jie-shouli DepartmentofGeneralSurgery WestChinaHospital610041 ChinicalSchoolofMedicalCollege NanjingUniversityNanjing210002

AIM: To establish a successful model of heterotopictotal small intestinal transplantation (SIT) in rats inorder to reduce the complications and increase thesurvival ***: A total of 196 Wistar rats underwentheterotopic SIT with microsurgical technique. Technicalmodifications included shortening fasting time andsupplying energy before surgery, administering optimalvolume of crystalloid fluid to the donor and recipientduring surgical procedures, reducing mechanical andischemic injuries to donor intestine, revascularizingsmall intestinal graft with a combination ofconventional aorta to aorta anastomosis and a cuffedportal vein to left renal vein anastomosis which resultedin an acceptably short warm ischemic time, and alsoan adequate blood supply and drainage of the ***: The average time for the donor surgery was86min±20min, the mean operative time for therecipient was 115min±20min and warm ischemia timewas shortened to 40min±5min. There was a shorterrevascularizing time of the graft, the abdominal aorta(AA) to AA anastomosis being 21min±10min, and thecuffed portal vein (PV) to the renal vein anastomosisbeing 5min±*** one-week survival rate of 98 ratswith SIT was 88.78% (87/98), without thrombosis andstenosis of anastomosis. The longest survival time ofrecipient rats was more than 389 days after SIT, therats were maintaining normal weight, with perfectintestinal function and intact intestinal ***: These modified techniques for SIT wouldremarkably reduce the complications and improve survival rate in rats, which provided a potentially more consistent and practical model for experimental andclinical studies.

关键词：异位全小肠移植技术改进动物实验并发症生存率

Protective effect of L-arginine preconditioning on ischemia and reperfusion injury associated with rat small bowel transplantation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2005年第19期11卷 2994-2997页

作者： BinCao NingLi YongWang jie-shouli DepartmentofSurgery NanjingUniversityMedicalSchoolTheFirstAffiliatedHospitalofNanjingMedicalUniversityNanjing210029JiangsuProvinceChina DepartmentofSurgery NanjingUniversityMedicalSchoolNanjing210002JiangsuProvinceChina

AIM: To investigate the protective effect and possible mechanism of L-arginine preconditioning on ischemia and reperfusion injury associated with small bowel transplantation (SBT).METHODS: Male inbred Wistar rats weighting between 180 and 250 g were used as donors and recipients in thestudy. Heterotopic rat SBT was performed according to the techniques of Li and Wu. During the experiment, intestinal grafts were preserved in 4 ℃ Ringer's solution for 8 h before being transplanted. Animals were divided into three groups. In group 1, donors received intravenous L-arginine (50 mg/kg, 1 mL) injection 90 min before graft harvesting. However, donors in control group were given normal saline (NS) instead. In group 3, six rats were used as sham-operated control. Specimens were taken from intestinal grafts 15 min after reperfusion. Histological grading, tissue malondialdehyde (MDA) and myeloperoxidase (MPO) levels were assessed. The graft survival of each group was monitored daily until 14 d after transplantation. RESULTS: Levels of MDA and MPO in intestine of shamoperated rats were 2.0±0.22 mmol/g and 0.66±0.105 U/g. Eight hours of cold preservation followed by 15 min of reperfusion resulted in significant increases in tissue MDA and MPO levels. Pretreatment with L-arginine before graft harvesting resulted in lower enhancement of tissue levels of MDA and MPO and the differences were significant (4.71±1.02 mmol/g vs8.02±3.49 mmol/g, 1.03±0.095 U/g vs 1.53±0.068 U/g, P＜0.05). Besides, animals in L-arginine pretreated group had better histological structures and higher 2-wk graft survival rates comparing with that in NS treated group (3.3±0.52 vs6±0.1, 0/6 vs6/6, P＜0.05or 0.01).CONCLUSION: L-arginine preconditioning attenuates ischemia and reperfusion injury in the rat SBT model,which was due to antioxidant activities partially.

关键词： L-arginine Small bowel transplantation

Effect of glutamine on change in early postoperative intestinal permeability and its relation to systemic inflammatory response

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2004年第13期10卷 1992-1994页

作者： Zhu-FuQuan ChongYang NingLi jie-shouli ResearchInstituteofGeneralSurgery JinlingHospitalMedicalCollegeofNanjingUniversityNanjing210002JiangsuProvinceChina

AIM: To study the effects of glutamine (Gin) on the change of intestinal permeability and its relationship to systemic inflammatory response in early abdominal postoperative ***: A prospective, randomized, double-blind and controlled trial was taken. Twenty patients undergoing abdominal surgery were randomized into Gin group (oral administration of glutamine, 30 g/d, for 7 d, n=10) and placebo group (oral administration of placebo, 30 g/d, for 7 d, n=-10). Temperatures and heart rates of all patients were daily recorded. White blood cell counts(WBC) and biochemical variables were measured before operation and 4 and 7 d alter drug administration. Serum concentrations of glutamine, endotoxin, diamine oxidase and malondialdehyde and urine lactulose/mannito (L/M) ratio were measured before and 7 d alter drug ***: The patients in the 2 groups were comparable prior to drug administration. Serum Gin concentration was significantly decreased in the placebo group and increased in the Gin group 7 d alter drug administration. Urine L/M ratio was significantly increased in the placebo group and decreased in the Gin group. The serum concentration of endotoxin, diamine oxidase and malondialdehyde was significantly decreased in the Gin group compared with those in the placebo group. Temperatures, heart rates and WBC counts were significantly lower in the Gin group than those in the placebo ***: Gut is one of the sources of systemic inflammatory response in abdominal postoperative patients and glutamine can decrease intestinal permeability, maintain intestinal barrier and attenuate systemic inflammatory response in early postoperative patients.

关键词：谷氨酰胺早期手术治疗肠渗透性系统性炎性反应腹部外科

Alterations of intestinal mucosa structure and barrier function following traumatic brain injury in rats

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2003年第12期9卷 2776-2781页

作者： Chun-HuaHang Ji-XinShi jie-shouli WeiWu Hong-XiaYin MedicalCollegeofNanjingUniversity DepartmentofNeurosurgery JinlingHospitalNanjing210002JiangsuProvinceChina ResearchInstituteofGeneralSurgery JinlingHospitalClinicalSchoolofMedicineNanjingUniversityNanjing210002JiangsuProvinceChina

AIM: Gastrointestinal dysfunction is a common complication in patients with traumatic brain injury (TBI). However, the effect of traumatic brain injury on intestinal mucosa has not been studied previously. The aim of the current study was to explore the alterations of intestinal mucosa morphology and barrier function, and to determine how rapidly the impairment of gut barrier function occurs and how long it persists following traumatic brain ***: Male Wistar rats were randomly divided into six groups (6 rats each group) including controls without brain injury and traumatic brain injury groups at hours 3,12, 24, and 72, and on day 7. The intestinal mucosa structure was detected by histopathological examination and electron microscopy. Gut barrier dysfunction was evaluated by detecting serum endotoxin and intestinal permeability. The level of serum endotoxin and intestinal permeability was measured by using chromogenic limulus amebocyte lysate and lactulose/mannitol (L/M) ratio, ***: After traumatic brain injury, the histopathological alterations of gut mucosa occurred rapidly as early as 3 hours and progressed to a serious state, including shedding of epithelial cells, fracture of villi, focal ulcer, fusion of adjacent villi, dilation of central chyle duct, mucosal atrophy,and vascular dilation, congestion and edema in the villous interstitium and lamina propria. Apoptosis of epithelial cells,fracture and sparseness of microvilli, loss of tight junction between enterocytes, damage of mitochondria and endoplasm, were found by electron microscopy. The villous height, crypt depth and surface area in jejunum decreased progressively with the time of brain injury. As compared with that of control group (183.7±41.8 EU/L), serum endotoxin level was signnificantly increased at 3, 12, and 24 hours following TBI (434.8±54.9 EU/L, 324.2±61.7 EU/L and 303.3±60.2 EU/L, respectively), and peaked at 72 hours (560.5±76.2 EU/L), then declined on day 7 (306.

关键词：创伤性脑损伤肠内粘膜肠内屏障胃肠道功能失调动物实验

Role of NF-κB and cytokine in experimental cancer cachexia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2003年第7期9卷 1567-1570页

作者： WeiZhou Zhi-WeiJiang jieTian JunJiang NingLi jie-shouli DepartmentofSurgery SclaoolofMedicineNanjingUniversityNaniing210002.Jian~suProvinceChina DepartmentofAnaesthesiology SchoolofLifeSciencesNaniinlzUniversityNaniing210002JiangsuProvinceChina

AIM: To assess the putative involvement of NF-κB and proinflammatory cytokines in the pathogenesis of cancer cachexia and the therapeutic efficacy of indomethacin (IND)on ***: Thirty young male BABL/c mice were divided randomly into five groups: (a) control, (b) tumor-bearing murine were inoculated subcutaneously to induce *** and IND were given intraperitoneally daily for 7 days from the onset of cachexia to sacrifice. Food intake and body composition were documented, serum levels of TNFα and IL-6 and activity of NF-κB in the spleen were investigated in all ***: Weight loss was observed in all tumor-bearing mice. By day 16, body weights of non-tumor mice were about 72 % of healthy controls (P＜0.01), and the weight of gastrocnemius was decreased by 28.7 % (P＜0.01). No difference was found between groups in food intake (P＞0.05).Gastrocnemius weight was increased markedly (P＜0.01)body weights were not significantly elevated. Tumor-bearing caused a 2-3 fold increase in serum levels of both TNF-αand IL-6 (P＜0.01). The concentration of TNF-α (P＜0.05)and IL-6 (P＜0.01) in tumor-bearing mice was reduced after of IL-6 was slightly elevated following treatment of IND 2.0tumor-bearing mice in comparison with controls in electrophoretic mobility shift assay (ENSA). NF-κB activity a higher NF-κB activity was observed in mice treated with CONCLUSION: Colon 26 adenocarcinoma cells can induce severe cancer cachexia experimentally, and the mechanism may be partially due to the enhanced TNF-αand IL-6 in tumor-bearing animals, which is controlled by NF-κB. Low dose of indomethacin alleviates the cachexia,decreases the activation of NF-κB and the serum levels of TNF-α and IL-6, and prevents body weight loss and muscle atrophy, while no further effect is gained by a higher dosage.

关键词：吲哚美辛恶病质核因子-κB 肿瘤坏死因子-α 白细胞介素-6

Retrovirus-mediated gene transfer of human endostatin inhibits growth of human liver carcinoma cells SMMC7721 in nude mice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2002年第6期8卷 1045-1049页

作者： XuanWang Fu-KunLiu XiLi jie-shouli Gen-XinXu DepartmentofMolecularBiology NanjingMilitaryMedicalSchoolNanjing210002JiangsuProvinceChina ResearchInstituteofGeneralSurgery ClinicalSchoolofMedicineNanjingUniversityNanjing210002JiangsuProvince

AIM: To study the effect of human endostatin mediated by retroviral gene transfer on the growth of human hepatocarcinoma cell line SMMC7721 in nude mice. METHODS: Human endostatin gene together with rat serum albumin signal peptide was transferred into human liver carcinoma SMMC7721 cells by retroviral vector pLncx to build a stable transfectant (SMMC-endo). PCR and Western blot analysis were used to verify the transfection and secretion of human endostatin gene in SMMC7721 cells. The endothelial cell proliferation assay in vitro was conducted to test the biological activity of the expressed human *** inhibitory effect of endostatin expressed by transfected 5MMC7721 on the growth rates of tumor cells in vivo was observed. The mean microvessel density in the specimen was also ***: PCR amplification proved that the genome of SMMC-endo cells contained a 550bp specific fragment of endostatin gene. Western blot analysis confirmed the secretion of human endostatin gene in the conditioned medium of transfected SMMC-endo cells. The endothelial proliferation assay showed that the conditioned medium of SMMC-endo cells significantly inhibited the proliferation of human umbilical vein endothelial cells by 48 %, significantly higher than that of SMMC-pLncx (10.2 %, P＜0.01). In vivo experiments revealed that only in 3 out of 5 mice tumors were formed and the mean size of flank tumors from SMMC-endo cells was 94.5 % smaller than that from the control SMMC-pLncx cells 22 days after tumor inoculation (P＜0.001).The mean microvessel density in tumor samples from SMMC-endo cells was only 8.6±1.1, much fewer than that of 22.6±4.5 from SMMC-pLncx cells (P＜0.01).CONCLUSION: Human endostatin mediated by retroviral gene transfer can inhibit human liver carcinoma cell SMMC7721 growth in nude mice.

关键词：逆转录病毒介导基因转染抗血管生成蛋白 Endostatin 裸鼠肝癌细胞生长 SMMC7721细胞系抑制作用

Changes of biological functions of dipeptide transporter (PepT1) and hormonal regulation in severe scald rats

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2003年第12期9卷 2782-2785页

作者： Bing-WeiSun Xiao-ChenZhao Guang-JiWang NingLi jie-shouli DepartmentofGeneralSurgery SchoolofMedicineNanjingUniversityNanjing210093JiangsuProvinceChina CenterofDrugMetabolismandPharmacokinetics ChinaPharmaceuticalUniversityNanjing210009JiangsuProvinceChina

AIM: To determine the regulatory effects of recombinant human growth hormone (rhGH) on dipeptide transport (PepT1) in normal and severe scald ***: Male Sprague-Dawley rats with 30 % total body surface area (TBSA) Ⅲ degree scald were employed as the model. In this study rhGH was used at the dose of 2 ***-1d-1. An everted sleeve of intestine 4 cm long obtained from mid-jejunum was securely incubated in Kreb's solution with radioactive dipeptide (3H-glycylsarcosine, 3H-Gly-Sar,10 μCi/ml) at 37 ℃ for 15 min to measure the effects of uptake and transport of PepT1 of small intestinal epithelial cells in normal and severe scald ***: Abundant blood supply to intestine and mesentery was observed in normal and scald rats administered rhGH,while less supply of blood to intestine and mesentery was observed in rats without rhGH. Compared with controls, the transport of dipeptide in normal rats with injection of rhGH was not significantly increased (P=0.1926), while the uptake was significantly increased (P=0.0253). The effects of transport and uptake of PepT1 in scald rats with injection of rhGH were significantly increased (P=0.0082, 0.0391).CONCLUSION: Blood supply to intestine and mesentery of rats was increased following injection of rhGH. The effects of uptake and transport of dipeptide transporters in small intestinal epithelial cells of rats with severe scald were markedly up-regulated by rhGH.

关键词：缩二氨酸转运物荷尔蒙严重烫伤动物实验作用机制

Operative stress response and energy metabolism after laparoscopic cholecystectomy compared to open surgery

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2003年第4期9卷 847-850页

作者： KaiLuo jie-shouli Ling-TangLi Kei-HuiWang Jing-MeiShun ResearchInstituteofGeneralSurgery NanjingGeneralhospitalNanjingCommandofPeople'sLiberationArmyandClinicalSchoolofMedicalCollegeNanjingUniversityNanjingJiangsuProvince210002China ResearchInstituteofGeneralSurgery NanjingGeneralhospitalNanjingCommandofPeople'sLiberationArmyandClinicalSchoolofMedicalCollegeNanjingUniversityNanjingJiangsuProvince210002China

AIM: To determine the least invasive surgical procedure by comparing the levels of operative stress hormones, responsereactive protein (CRP) and rest energy expenditure (REE)after laparoscopic (LC) and open cholecystectomy (OC).METHODS: Twenty-six consecutive patients with noncomplicated gallstones were randomized for LC (14) and OC (12). Plasma concentrations of somatotropin, insulin, cortisol and CRP were measured. The levels of REE were ***: In the third postoperative day, the insulin levels were lower compared to that before operation (P＜0.05).Tn the first postoperative day, the levels of somatotropin and cortisol were higher in OC than those in LC. After operation the parameters of somatotropin, CRP and cortisol increased, compared to those in the preoperative period in the all patients (P＜0.05). In the all-postoperative days,the CRP level was higher in OC than that in LC (7.46±0.02;7.38±0.01, P＜0.05). After operation the REE level all increased in OC and LC (P＜0.05). In the all-postoperative days, the REE level was higher in OC than that in LC (1438.5±A18.5;1222.3±L80.8, P＜0.05).CONCLUSION: LC results in less prominent stress response and smaller metabolic interference compared to open surgery. These advantages are beneficial to the restoration of stress hormones, the nitrogen balance, and the energy metabolism. However, LC can also induce acidemia and pulmonary hypoperfusion because of the penumoperitonium it uses during surgery.

关键词：腹腔镜胆囊切除术开腹手术手术应激能量代谢微创外科胆道疾病