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LncRNAs expression signatures of hepatocellular carcinoma revealed by microarray

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World Journal of Gastroenterology 2014年第20期20卷 6314-6321页

作者： Wu-Tao Liu Xin Lu Guang-Hua Tang Jin-Jun Ren Wen-Jun Liao Peng-Lei Ge jie-fu huang Department of Liver Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College

AIM: To analyze the expression profiles of long non-coding RNAs (lncRNAs) in hepatocellular carcinoma.

关键词： Long non-coding RNA Hepatocellular carcinoma MALAT1 Expression signature Microarray

Effect of preoperative transcatheter arterial chemoembolization on proliferation of hepatocellular carcinoma cells

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World Journal of Gastroenterology 2007年第33期13卷 4509-4513页

作者： En-Hua Xiao Jing-Qing Li jie-fu huang Department of Radiology the Second Xiangya Hospital Central South University Changsha 410011 Hunan Province China Liver Cancer Research Center Sun Yat-sen University of Medical Sciences Guangzhou 510060 Guangdong

AIM： To evaluate the effect of preoperative transcatheter arterial chemoembolization （TACE） on proliferation of hepatocellular carcinoma （HCC） cells. METHODS： A total of 136 patients with HCC underwent liver resection. Of 136 patients, 79 patients received 1 to 5 courses of TACE prior to liver resection （TACE group）, who were further subdivided into four groups： Group A （n = 11） who received i to 4 courses of chemotherapy alone; Group B （n = 33） who received i to 5 courses of chemotherapy combined with iodized oil; Group C （n = 23） who received i to 3 courses of chemotherapy combined with iodized oil and gelatin sponge; and Group D （n = 12） who received 1 to 3 courses of chemotherapy combined with iodized oil, ethanol and gelatin sponge. The other 57 patients only received liver resection （non- TACE group）. The expressions of Ki-67 and proliferating cell nuclear antigen （PCNA） protein were detected in the liver cancer tissues by immunohistochemical method. RESULTS： The Ki-67 protein expression was significantly lower in Groups C and D as compared with non-TACE group （31.35% ± 10.85% vs 44.43% ± 20.70%, 30.93% ± 18.10% vs 44.43% ± 20.70%, respectively, P 〈 0.05）. The PCNA protein expression was significantly lower in Groups C and D as compared with non-TACE group （49.61% ± 15.11% vs 62.92% ± 17.21%, 41.16% ± 11.83% vs 62.92% ± 17.21%, respectively, P 〈 0.05）. The Ki-67 protein expression was significantly higher in Group A as compared with non-TACE group （55.44% ± 13.72% vs 44.43% ± 20.70%, P 〈 0.05）. The PCNA protein expression was significantly higher in Groups A and B as compared with non-TACE group （72.22% ± 8.71% vs 62.92% ± 17.21%, 69.91% ± 13.38% vs 62.92% ± 17.21%, respectively, P 〈0.05）. CONCLUSION： Preoperative multi-material TACE suppresses the proliferation of HCC cells, while a single material embolization and chemotherapy alone enhance the proliferation of HCC cells.

关键词： Hepatocellular carcinoma Transcatheter arterial chemoembolization Proliferating cell nuclear antigen Ki-67

China organ donation and transplantation update: the Hangzhou Resolution

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Hepatobiliary & Pancreatic Diseases International 2014年第2期13卷 122-124页

作者： jie-fu huang Shu-Sen Zheng Yong-Feng Liu Hai-Bo Wang Jeremy Chapman Philip O'Connell Michael Millis John fung Francis Delmonico Department of Liver Surgery Peking Union Medical College Hospital National Organ Transplant Committee Ministry of Health The First Affiliated Hospital of Zhejiang University The First Hospital of China Medical University Department of Surgery The University of Hong Kong China Organ Transplant Response System Research Center Centre for Renal and Transplant Research Westmead Millenium InstituteSydney University Section of Transplantation Liver Transplantation and Hepatobiliary SurgeryUniversity of Chicago Department of General Surgery Transplant CenterDigestive Disease Institute Department of Surgery Massachusetts General Hospital

The much-anticipated change in the practice of organ donation and transplantation in China is now underway and affirmed by an important Hangzhou Resolution promulgated at the 2013 China Transplant *** of the National Health and Family Planning Commission On October 29,2013,in a meeting of the National Health and Family Planning Commission (NHFPC)officials with jie-fu huang,Head of National Organ Transplant Committee(OTC), Hai-Bo Wang,Director of China Organ Transplant Response System (COTRS)

关键词： Li China organ donation and transplantation update the Hangzhou Resolution Jun

SCF-CNDO/2-CO STUDY ON NITROGEN-CONTAINING ANALOGS OF POLYACETYLENE

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Chinese Chemical Letters 1992年第2期3卷 99-102页

作者： jie fu, Yuan He huang, Ruo Zhuang LIU Department of Chemistry, Beijing Normal University, Beijing 100875

The electronic structures of polymethineimine (PMI), polyazine (PAZ) and polyazoethene(PAE) are studied on the basis of the tight- binding LCAO -8CF-CO calculations under the CNDO/2 approximations. Our result indicates that they are all poor candidates for new electric conducting materials as alternatives to polyacetylene(PA), and this is discussed in terms of the electronic structures. PMI end PAZ have been synthesized successfully, and some of their properties have been investigated. In this article we intend to explain why and how degree of conjugation effects on their intrinsic electric conductivities by studying their electronic structures on the basis of the tight-binding LCAO-SCF-CO calculations including all valence atomic orbitals(AO'S) with complete neglect of differential overlap(CNDO/2) approximations.

关键词： CNDO IT PAE SCF-CNDO/2-CO STUDY ON NITROGEN-CONTAINING ANALOGS OF POLYACETYLENE CO

Hepatocyte apoptosis induced by hepatic ischemia-reperfusion injury in cirrhotic rats

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Hepatobiliary & Pancreatic Diseases International 2003年第1期2卷 102-105页

作者： Shao-Qiang Li Li-Jian Liang jie-fu huang Zhi Li the Departments of Hepatobiliary Surgeryand Pathology First Affiliated Hospital, Zhongshan University, Guangzhou 510080, China Departments of Hepatobiliary Surgery First Affi-liated Hospital Zhongshan University Guangzhou 510080 China. lesqch@***

OBJECTIVE: To investigate whether hepatocyte apoptosis in hepatic ischemia-reperfusion (I/R) injury is mediated by Fas pathway. METHODS: Fas-mRNA expression detected by in situ hybridization assay, caspase-3 activity measured by fluorescence spectrophotometer, and hepatocyte apoptosis detected by TUNEL assay were compared in different L/R conditions between cirrhotic and normal rats. The relationship was analyzed between hepatocyte apoptosis, Fas-mRNA expression, and caspase-3 activity. RESULTS: In cirrhotic rats, Fas-mRNA expression and caspase-3 activity were significantly increased when the ischemic time prolonged, and subsequently, hepatocyte apoptosis was increased (P<0.O1). Under the same I/R condition, the Fas-mRNA expression, caspase-3 activity and hepatocyte apoptosis in cirrhotic liver were significantly higher than those in normal liver (P<0.O1). CONCLUSIONS: Hepatocyte apoptosis in hepatic I/R injury might be mediated by Fas pathway. The possible underlying mechanism that cirrhotic liver is more sensitive to ischemic injury than normal liver is alteration of Fas expression level.

关键词： ischemia-reperfusion apoptosis cirrhosis Fas caspase-3

Ru doping triggering reconstruction of cobalt phosphide for coupling glycerol electrooxidation with seawater electrolysis

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Journal of Energy Chemistry 2025年第1期100卷 317-326页

作者： Binglu Deng jie Shen Jinxing Lu Chuqiang huang Zhuoyuan Chen Feng Peng Yunpeng Liu School of Materials Science and Hydrogen Energy Foshan University School of Chemistry and Chemical Engineering Guangzhou University Guangdong Key Laboratory for Hydrogen Energy Technologies

Seawater electrolysis is a promising approach for sustainable energy without relying on precious ***,the large-scale seawater electrolysis is hindered by low catalytic efficiency and severe anode corrosion caused by the harmful *** contrast to the oxygen evolution reaction （OER）and chlorin ion oxidation reaction （ClOR）,glycerol oxidation reaction （GOR） is more thermodynamically and kinetically favorable ***,a Ru doping cobalt phosphide （Ru-CoP2） is proposed as a robust bifunctional electrocatalyst for seawater electrolysis and GOR,for the concurrent productions of hydrogen and value-added *** in situ and ex situ characterization analyses demonstrated that Ru doping featured in the dynamic reconstruction process from Ru-CoP2to Ru-CoOOH,accounting for the superior GOR *** coupling GOR with hydrogen evolution was realized by employing Ru-CoP2as both anode and cathode,requiring only a low voltage of 1.43 V at 100 mA cm-2,which was 250 m V lower than that in alkaline *** work guides the design of bifunctional electrocatalysts for energy-efficient seawater electrolysis coupled with biomass resource upcycling.

关键词： Anodes

Surgical resection plus biotherapy/chemotherapy improves survival of hepatic metastatic melanoma

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World Journal of Hepatology 2012年第11期4卷 305-310页

作者： Shun-Da Du Yi-Lei Mao Shao-Hua Li Xin-Ting Sang et al. Xin Lu Yi-Yao Xu Hai-Feng Xu Lin Zhao Chun-Mei Bai Shou-Xian Zhong jie-fu huang Shun-Da Du Yi-Lei Mao Shao-Hua Li Xin-Ting Sang Xin Lu Yi-Yao Xu Hai-Feng Xu Shou-Xian Zhong Jie-Fu Huang Department of Liver Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730 China.

AIM: To analyze the correlation of treatment method with the outcome of all the hepatic metastatic mela-noma (HMM) patients from our ***: There were altogether nine cases of HMM that had been treated in the PUMCH hospital during the past 25 years, from December 1984 to February 2010. All of the cases developed hepatic metastasis from primary cutaneous melanoma. A retrospective review was performed on all the cases in order to draw informative conclusion on diagnosis and treatment in correlation with the prognosis. Clinical features including symptoms, signs, blood test results, Bultrasound and computed tomography (CT) imaging characteristics, and pathological data were analyzed in each case individually. A simple comparison was made on case by case basis instead of performing statistical analysis since the case numbers are low and patients were much diversified in each item that has been analyzed. Literatures on this subject were reviewed in order to draw a safe conclusion and found to be supportive to our finding in a much broad scope. RESULTS: There are six males and three females whose ages ranged 39-74 years old with an average of 58.8. Patients were either with or without symptoms at the time of diagnosis. The liver function and tumor marker exam were normal in all but one patient. The incidence of HMM does not affect liver function and was not related to virus infection status in the liver. Most of these HMM patients were also accompanied by the metastases of other locations, including lung, abdominal cavity, and cervical lymph nodes. Ultra-sound examinations showed lesions ranging 2-12 cm in diameter, with noor low-echo peripheral areola. Doppler showed blood flow appeared inside some tumors as well as in the surrounding area. CT image demonstrated low density without uniformed lesions, characterized with calcification in periphery, and en-hanced in the arterial phase. Contrast phase showed heterogeneous enhancement, with a density higher than no

关键词： Malignant melanoma Hepatic metastatic tumor Hepatectomy Hepatic metastatic melanoma Prognosis Biotherapy Chemotherapy

Influence of warm ischemia injury on hepatic functional status and survival of liver graft in rats

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Hepatobiliary & Pancreatic Diseases International 2003年第4期2卷 504-508页

作者： Xiao-Shun He Yi Ma Lin-Wei Wu Wei-Qiang Ju Gui-Hua Chen Rui-De Hu jie-fu huang the Organ Transplantation Center, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China Organ Transplantation Center First Affiliated Hospital of Sun Yat-Sen University Guangzhou 510080 China. xshe@***

OBJECTIVES: To investigate the changing patterns of functional and histological status, observe the posttransplantation survival of liver graft under different warm ischemia time (WIT) in rats, and determine the maximum limitation of liver graft to warm ischemia. METHODS: According to WIT, the rats were randomized into 7 groups, with WIT of 0, 10, 15, 20, 30, 45, 60 minutes respectively. Serum concentrations of alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase were measured at 1, 2, 3 and 5 days after orthotopic liver transplantation respectively. Liver graft specimens were observed histopathologically at the same interval. The rats' survival in each subgroup was observed. RESULTS: In terms of graft survival, there was no significant difference between subgroups within 30-minute WIT. In the group with 30-minute WIT, the recipient rats' survival rate was 83.3% (10/12) at one week, 58.3% (7/12) at one month, and 50.0% (6/12) at 3 months. In the group with 45-minute WIT, the recipient rats' survival rate was 66.7% (8/12) at one week, 33.3% (4/12) at one month, and 8.3% (1/12) at 3 months, whereas only 8.3% (1/12) of the rats had one-week survival in the group with 60-minute WIT. CONCLUSIONS: These results indicate that rat liver graft could be safely subject to warm ischemia within 30 minutes. When WIT is prolonged to 45 minutes, the recipients long-term survival is severely insulted, and both function and histological structure of liver graft may develop irreversible damage when WIT is prolonged to 60 minutes.

关键词： liver transplantation warm ischemia injury survival

Dynamic microcirculatory changes in liver graft from non-heart-beating donor with warm ischemia injury in rat

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Hepatobiliary & Pancreatic Diseases International 2004年第2期3卷 179-182页

作者： Xiao-Shun He, Yi Ma, Wei-Qiang Ju, Lin-Wei Wu, Jin-Lang Wu, Ying-jie Liang, Rui-De Hu, Gui-Hua Chen and jie-fu huang Guangzhou, China Organ Transplantation Center, First Affiliated Hospital and Depart- ment of Pathology, Sun Yat-Sen University, Guang- zhou 510080, China Organ Transplantation Center First Affiliated Hospital Sun Yat-Sen University Guangzhou 510080 China. xshe@***

BACKGROUND: Since the 1990s, liver grafts from non- heart-beating donor (NHBD) have become an alternative because of the deficiency of grafts from heart-beating-do- nors (HBDs). Warm ischemia injury, however, directly influences the grafts' activity and functional recovery after operation. We investigated the microcirculatory change of liver graft at different warm ischemia time (WIT) in rats and determined the maximum limitation of liver graft to warm ischemia. METHODS: According to WIT, 120 rats were divided ran- domly into 5 groups of 0, 15 , 30 , 45 , 60 minutes respec- tively. The microcirculatory changes of their liver grafts were measured including serum level of hyaluronic acid (HA) and ultrastructural changes. After orthotopic liver transplantation (OLT), the recovery of microcirculation of the liver grafts after 24 hours, 48 hours and 3 days was ob- served. RESULTS: Microcirculatory changes and function of the liver grafts became normal after reperfusion when the WIT was less than 30 minutes. In the 45-minute WI group, part of blood sinusoids was full of cytoplasmic blebs stemming from the microvilli of hepatocytes and hemocytes. The se- rum level of HA in each group after 45 minutes of WI re- covered after reperfusion. CONCLUSIONS: The microcirculatory change of rat liver graft is reversible when the WIT is less than 30 minutes: rat liver graft could be safely subject to warm ischemia within30 minutes. The maximal 45 minutes of WI can be tolera- ted by the microcirculatory function of liver graft. After 60 minutes of WI, irreversible disturbance of microcirculation may appear.

关键词： liver transplantation warm ischemia injury microcirculatory change