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Horticulture Research 2020年第1期7卷 2395-2403页

作者： chen-Kai Jiang Jian-Qiang Ma Yu-Fei Liu jie-dan chen De-Jiang Ni Liang chen Key Laboratory of Tea Biology and Resources Utilization Ministry of Agriculture and Rural AffairsTea Research Institute of the Chinese Academy of Agricultural Sciences9 South Meiling RoadHangzhouZhejiang 310008China College of Horticulture and Forestry Science Huazhong Agricultural University1 Shizishan StreetHongshan DistrictWuhanHubei 430070China Tea Research Institute Yunnan Academy of Agricultural SciencesMenghaiYunnan 666201China

Catechins are the predominant products in tea plants and have essential functions for both plants and *** genes encoding the enzymes regulating catechin biosynthesis have been identified,and the identification of single nucleotide polymorphisms(SNPs)resulting in nonsynonymous mutations within these genes can be used to establish a functional link to catechin ***,the transcriptomes of two parents and four filial offspring were sequenced using next-generation sequencing technology and aligned to the reference genome to enable SNP ***,176 tea plant accessions were genotyped based on candidate SNPs using kompetitive allelespecific polymerase chain reaction(KASP).The catechin contents of these samples were characterized by highperformance liquid chromatography(HPLC),and analysis of variance(ANOVA)was subsequently performed to determine the relationship between genotypes and catechin *** a result of these efforts,a SNP within the chalcone synthase(CHS)gene was shown to be functionally associated with catechin ***,the geographical and interspecific distribution of this SNP was ***,these results will contribute to the early evaluation of tea plants and serve as a rapid tool for accelerating targeted efforts in tea breeding.

关键词： distribution content mining

The chromosome-scale genome reveals the evolution and diversification after the recent tetraploidization event in tea plant

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Horticulture Research 2020年第1期7卷 2017-2027页

作者： jie-dan chen Chao Zheng Jian-Qiang Ma chen-Kai Jiang Sezai Ercisli Ming-Zhe Yao Liang chen Key Laboratory of Tea Biology and Resources Utilization Ministry of Agriculture and Rural AffairsTea Research Institute of the Chinese Academy of Agricultural ScienceHangzhou 310008China Department of Horticulture Faculty of AgricultureAtaturk UniversityErzurumTurkey

Tea is one of the most popular nonalcoholic beverages due to its characteristic secondary metabolites with numerous health *** two draft genomes of tea plant(Camellia sinensis)have been published recently,the lack of chromosome-scale assembly hampers the understanding of the fundamental genomic architecture of tea plant and potential ***,we performed a genome-wide chromosome conformation capture technique(Hi-C)to obtain a chromosome-scale assembly based on the draft genome of *** *** and successfully ordered 2984.7 Mb(94.7%)scaffolds into 15 *** scaffold N50 of the improved genome was 218.1 Mb,~157-fold higher than that of the draft *** comparison of genome sequences and two genetic maps validated the high contiguity and accuracy of the chromosome-scale *** clarified that only one Camellia recent tetraploidization event(CRT,58.9–61.7 million years ago(Mya))occurred after the core-eudicot common hexaploidization event(146.6–152.7 Mya).Meanwhile,9243 genes(28.6%)occurred in tandem duplication,and most of these expanded after the CRT *** gene duplicates increased functionally divergent genes that play important roles in tea-specific biosynthesis or stress ***-four catechin-and caffeine-related quantitative trait loci(QTLs)were anchored to chromosome *** these,two catechin-related QTL hotspots were derived from the CRT event,which illustrated that polyploidy has played a dramatic role in the diversification of tea *** availability of a chromosome-scale genome of tea plant holds great promise for the understanding of genome evolution and the discovery of novel genes contributing to agronomically beneficial traits in future breeding programs.

关键词： breeding evolution holds

Deeply functional identification of TCS1 alleles provides efficient technical paths for low-caffeine breeding of tea plants

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Horticulture Research 2023年第2期10卷 266-274页

作者： Yi Wang Yu-Fei Liu Meng-Yuan Wei chen-Yu Zhang jie-dan chen Ming-Zhe Yao Liang chen Ji-Qiang Jin Key Laboratory of Biology Genetics and Breeding of Special Economic Animals and PlantsMinistry of Agriculture and Rural AffairsTea Research Institute of the Chinese Academy of Agricultural SciencesHangzhou 310008China Yunnan Provincial Key Laboratory of Tea Science Tea Research InstituteYunnan Academy of Agricultural Sciences2 Jingnan RoadMenghaiYunnan 666201China

Caffeine is an important functional component in tea,which has the effect of excitement and nerve stimulation,but excessive intake can cause insomnia and ***,the production of tea with low-caffeine content can meet the consumption needs of certain ***,in addition to the previous alleles of the tea caffeine synthase(TCS1)gene,a new allele(TCS1h)from tea germplasms was *** of in vitro activity analysis showed that TCS1h had both theobromine synthase(TS)and caffeine synthase(CS)***-directed mutagenesis experiments of TCS1a,TCS1c,and TCS1h demonstrated that apart from the 225th amino acid residue,the 269th amino acid also determined the CS *** histochemical analysis and dual-luciferase assay indicated the low promoter activity of TCS1e and *** parallel,insertion and deletion mutations in large fragments of alleles and experiments of site-directed mutagenesis identified a key cis-acting element(G-box).Furthermore,it was found that the contents of purine alkaloids were related to the expression of corresponding functional genes and alleles,and the absence or presence and level of gene expression determined the content of purine alkaloids in tea plants to a certain *** summary,we concluded TCS1 alleles into three types with different functions and proposed a strategy to effectively enhance low-caffeine tea germplasms in breeding *** research provided an applicable technical avenue for accelerating the cultivation of specific low-caffeine tea plants.

关键词： functional alkaloids directed

Increased excitatory amino acid transporter 2 levels in basolateral amygdala astrocytes mediate chronic stress–induced anxiety-like behavior

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Neural Regeneration Research 2025年第6期20卷 1721-1734页

作者： Xirong Xu Shoumin Xuan Shuai chen dan Liu Qian Xiao jie Tu Shenzhen Key Laboratory of Neuroimmunomodulation for Neurological Diseases Shenzhen-Hong Kong Institute of Brain ScienceShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina CAS Key Laboratory of Brain Connectome and Manipulation Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Brain Connectome and Behavior the Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina University of Chinese of Academy of Sciences BeijingChina Faculty of Life and Health Sciences Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina

The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with ***, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal *** knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was ob

关键词： anxiety astrocytes basolateral amygdala behavior dihydrokainic acid excitatory amino acid transporter 2 fiber photometry glutamate LDN-212320 transporter

Enhancement of motor functional recovery in thoracic spinal cord injury: voluntary wheel running versus forced treadmill exercise

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Neural Regeneration Research 2025年第3期20卷 836-844页

作者： Do-Hun Lee dan Cao Younghye Moon chen chen Nai-Kui Liu Xiao-Ming Xu Wei Wu Spinal Cord and Brain Injury Research Group Stark Neurosciences Research InstituteIndiana University School of MedicineIndianapolisINUSA Department of Neurological Surgery Indiana University School of MedicineIndianapolisINUSA Department of Neuroscience Baylor College of MedicineHoustonTXUSA

Spinal cord injury necessitates effective rehabilitation strategies, with exercise therapies showing promise in promoting recovery. This study investigated the impact of rehabilitation exercise on functional recovery and morphological changes following thoracic contusive spinal cord injury. After a 7-day recovery period after spinal cord injury, mice were assigned to either a trained group(10 weeks of voluntary running wheel or forced treadmill exercise) or an untrained group. Bi-weekly assessments revealed that the exercise-trained group, particularly the voluntary wheel exercise subgroup, displayed significantly improved locomotor recovery, more plasticity of dopaminergic and serotonin modulation compared with the untrained group. Additionally, exercise interventions led to gait pattern restoration and enhanced transcranial magnetic motor-evoked potentials. Despite consistent injury areas across groups, exercise training promoted terminal innervation of descending axons. In summary, voluntary wheel exercise shows promise for enhancing outcomes after thoracic contusive spinal cord injury, emphasizing the role of exercise modality in promoting recovery and morphological changes in spinal cord injuries. Our findings will influence future strategies for rehabilitation exercises, restoring functional movement after spinal cord injury.

关键词： behavioral assessment motor function neural plasticity running wheel exercise spinal cord injury treadmill exercise voluntary exercise

The emerging role of nitric oxide in the synaptic dysfunction of vascular dementia

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Neural Regeneration Research 2025年第2期20卷 402-415页

作者： Xiaorong Zhang Zhiying chen Yinyi Xiong Qin Zhou Ling-Qiang Zhu dan Liu Department of Pathology Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Jiujiang Clinical Precision Medicine Research Center JiujiangJiangxi ProvinceChina Center for Cognitive Science and Transdisciplinary Studies Jiujiang UniversityJiangxi ProvinceChina Department of Neurology Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Department of Rehabilitation Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Department of Pathophysiology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

With an increase in global aging,the number of people affected by cerebrovascular diseases is also increasing,and the incidence of vascular dementia-closely related to cerebrovascular risk-is increasing at an epidemic ***,few therapeutic options exist that can markedly improve the cognitive impairment and prognosis of vascular dementia *** in Alzheimer’s disease and other neurological disorders,synaptic dysfunction is recognized as the main reason for cognitive *** oxide is one of the ubiquitous gaseous cellular messengers involved in multiple physiological and pathological processes of the central nervous ***,nitric oxide has been implicated in regulating synaptic plasticity and plays an important role in the pathogenesis of vascular *** review introduces in detail the emerging role of nitric oxide in physiological and pathological states of vascular dementia and summarizes the diverse effects of nitric oxide on different aspects of synaptic dysfunction,neuroinflammation,oxidative stress,and blood-brain barrier dysfunction that underlie the progress of vascular ***,we propose that targeting the nitric oxide-sGC-cGMP pathway using certain specific approaches may provide a novel therapeutic strategy for vascular dementia.

关键词： endoplasmic reticulum stress endothelial nitric oxide synthase gene therapy nitric oxide NO-sGC-cGMP pathway synaptic dysfunction vascular dementia

Role of iron ore in enhancing gasification of iron coke:Structural evolution,influence mechanism and kinetic analysis

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 58-69页

作者： jie Wang Wei Wang Xuheng chen Junfang Bao Qiuyue Hao Heng Zheng Runsheng Xu School of Chemistry and Materials Science Hubei Engineering UniversityXiaogan 432000China Key Laboratory for Ferrous Metallurgy and Resources Utilization of Ministry of Education Wuhan University of Science and TechnologyWuhan 430081China Institue of Ironmaking Technology Baosteel Central Research InstituteShanghai 201999China State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China

The utilization of iron coke provides a green pathway for low-carbon *** uncover the influence mechanism of iron ore on the behavior and kinetics of iron coke gasification,the effect of iron ore on the microstructure of iron coke was ***,a comparative study of the gasification reactions between iron coke and coke was conducted through non-isothermal thermogravimetric *** findings indicate that compared to coke,iron coke exhibits an augmentation in micropores and specific surface area,and the micropores further extend and *** provides more adsorption sites for CO_(2) molecules during the gasification process,resulting in a reduction in the initial gasification temperature of iron *** the heating rate in non-isothermal gasification can enhance the reactivity of iron *** metallic iron reduced from iron ore is embedded in the carbon matrix,reducing the orderliness of the carbon structure,which is primarily responsible for the heightened reactivity of the carbon *** kinetic study indicates that the random pore model can effectively represent the gasification process of iron coke due to its rich pore ***,as the proportion of iron ore increases,the activation energy for the carbon gasification gradually decreases,from 246.2 kJ/mol for coke to 192.5 kJ/mol for iron coke 15wt%.

关键词： low-carbon ironmaking iron coke gasification structural evolution kinetic model

Cyano migration-mediated radical-polar crossover cyclopropanation

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Science China Chemistry 2025年第01期 241-248页

作者： Ziqiang Wang Yasu chen jie Li chen Zhu Key Laboratory of Organic Synthesis of Jiangsu Province College of Chemistry Chemical Engineering and Materials ScienceSoochow University Frontiers Science Center for Transformative Molecules Zhangjiang Institute for Advanced Study and Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs Shanghai Jiao Tong University

We report a novel cyano migration-mediated radical-polar crossover process, whereby both the sulfonylcyanation of aliphatic alkenes and the formation of cyclopropanes are concurrently achieved. This transformation yields a diverse array of multisubstituted cyclopropanes under mild photocatalytic conditions. Mechanistic studies elucidate that the reaction proceeds via intramolecular nucleophilic substitution, leading to the formation of the cyclopropane derivatives.

关键词：

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Ru doping triggering reconstruction of cobalt phosphide for coupling glycerol electrooxidation with seawater electrolysis

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Journal of Energy Chemistry 2025年第1期100卷 317-326页

作者： Binglu Deng jie Shen Jinxing Lu Chuqiang Huang Zhuoyuan chen Feng Peng Yunpeng Liu School of Materials Science and Hydrogen Energy Foshan University School of Chemistry and Chemical Engineering Guangzhou University Guangdong Key Laboratory for Hydrogen Energy Technologies

Seawater electrolysis is a promising approach for sustainable energy without relying on precious ***,the large-scale seawater electrolysis is hindered by low catalytic efficiency and severe anode corrosion caused by the harmful *** contrast to the oxygen evolution reaction （OER）and chlorin ion oxidation reaction （ClOR）,glycerol oxidation reaction （GOR） is more thermodynamically and kinetically favorable ***,a Ru doping cobalt phosphide （Ru-CoP2） is proposed as a robust bifunctional electrocatalyst for seawater electrolysis and GOR,for the concurrent productions of hydrogen and value-added *** in situ and ex situ characterization analyses demonstrated that Ru doping featured in the dynamic reconstruction process from Ru-CoP2to Ru-CoOOH,accounting for the superior GOR *** coupling GOR with hydrogen evolution was realized by employing Ru-CoP2as both anode and cathode,requiring only a low voltage of 1.43 V at 100 mA cm-2,which was 250 m V lower than that in alkaline *** work guides the design of bifunctional electrocatalysts for energy-efficient seawater electrolysis coupled with biomass resource upcycling.

关键词： Anodes

Dynamic behaviors of graphene platelets-reinforced metal foam piezoelectric beams with velocity feedback control

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Applied Mathematics and Mechanics(English Edition) 2025年第1期46卷 63-80页

作者： jie chen Xinyue ZHANG Mingyang FAN School of Mathematics Statistics and Mechanics Beijing University of Technology College of Mechanical & Energy Engineering Beijing University of Technology

Graphene platelets(GPLs)-reinforced metal foam structures enhance the mechanical properties while maintaining the lightweight characteristics of metal foams. Further bonding piezoelectric actuator and sensor layers on the surfaces of GPLs-reinforced metal foam beams enables active vibration control, greatly expanding their applications in the aerospace industry. For the first time, this paper investigates the vibration characteristics and active vibration control of GPLs-reinforced metal foam beams with surfacebonded piezoelectric layers. The constant velocity feedback scheme is used to design the closed-loop controller including piezoelectric actuators and sensors. The effects of the GPLs on the linear and nonlinear free vibrations of the beams are numerically *** Newmark-β method combined with Newton's iteration technique is used to calculate the nonlinear responses of the beams under different load forms including harmonic loads,impact loads, and moving loads. Additionally, special attention is given to the vibration reduction performance of the velocity feedback control on the responses of the beam.

关键词： Piezoelectric actuators