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Identification,localization and morphology of APUD cells in gastroenteropancreatic system of stomach-containing teleosts

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World Journal of Gastroenterology 2000年第6期6卷 842-847页

作者： Qian Sheng Pan Zhi Ping Fang feng jie huang College of Fisheries Huazhong Agricultural UniversityWuhan 430070Hubei ProvinceChina College of Animal Husbandry and Veterinary Medicine Huazhong Agricultural UniversityWuhan 430070Hubei ProvinceChina

AIM To identify the type localization andmorphology of APUD endocrine cells in thegastroenteropancreatic(GEP)system ofstomach-containing teleosts,and study APUDendocrine system in the stomach,intestine andpancreas of fish *** Two kinds of immunocytochemical(ICC)techniques of the streptavidin biotin-peroxidase complex(SABC)and streptavidin-peroxidase(S-P)method were ***,localization and morphology ofAPUD endocrine cells scattered in the mucosa ofdigestive tract,intermuscular nerve plexus andglandular body of northern snakehead(Channaargus),ricefield eel(Monopterus albus),yellow catfish(Pelteobagrus fulvidraco),mandarinfish(Siniperca chuatsi),largemouthbass(Micropterus salmoides),orientalsheatfish(Silurus asotus),freshwater pomfret(Colossoma brachypomum)and nile tilapia(Tilapia nilotica)were investigated with 8 kindsof *** The positive reaction of 5-hydroxytryptamine(5-HT)immunoreactiveendocrine(IRE)cells was found in the digestive tract and glandular body of 8 fish species indifferent *** a few gastrin(GAS)-IREcells were seen in ***,*** and ***(GLU)-IRE cells were notfound in the digestive tract and glandular bodybut existed in pancreatic island of most *** positive reaction of growthhormone(GH)-IRE cells was found only inpancreatic island of *** and ***,no positive reaction in the other 6 fish ***(SOM)-,calcitonin(CAL)-,neurofilament(NF)-and insulin(INS)-IRE cellsin the stomach,intestine and pancreas of 8 kindsof fish were different in distribution and *** distribution of all 8 APUD cells was the mostin gastrointestinal epithelium mucosa and then indigestive *** positive reaction of SOM-and 5-HT-IRE cells was found in intermuscularnerve plexus of intestine of *** *** GH-IRE cells were denselyscattered in the pancreatic islands of *** ***,and odd distribution in thepancreas of ***,SOM-IRE cells we

关键词： stomach-containing teleosts gastroenteropancreatic system APUD cells immunocytochemistry

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Expression and significance of MMP3 in synovium of knee joint at different stage in osteoarthritis patients

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Asian Pacific Journal of Tropical Medicine 2014年第4期7卷 297-300页

作者： Jun-jie Chen jie-feng huang Wen-Xi Du Pei-Jian Tong Department of Orthopedics First Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine

Objective:To investigate the expression and significance of MMP-3 in synovium of knee joint at different stage in osteoarthritis(OA)***:Knee synovial tissue were collected in90 OA patients(the OA group).Patients in the OA group was divided into 3 subgroups:gradeⅠsubgroup(n=30),gradeⅡsubgroup(n=30),gradeⅢsubgroup(n=30).Thirty patients served as control group,lmmunohistochemical assay was used to detect the expression of MMP-3 protein in the knee synovial ***:MMP-3 protein was detected in all knee synovial *** expression of MMP-3 protein in the OA group was significantly higher that in the normal synovium(P<0.05),and the MMP-3 protein was mainly located in the *** was significant difference in the expression of MMP-3 protein between the gradeⅢsubgroup and the gradeⅠ,gradeⅡsubgroups(all P<0.05).The expression of MMP-3 protein was positively related to the severity of OA(r=0.912,P<0.05).Conclusions:The expression of MMP-3 protein are closely related to pathogenic mechanism of *** may be an important indicator of early diagnosis and the activity of the disease of osteoarthritis.

关键词： Osteoarthritis MMP-3 Immunohistochemical

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Total knee arthroplasty in Ranawat Ⅱ valgus deformity with enlarged femoral valgus cut angle:A new technique to achieve balanced gap

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World Journal of Clinical Cases 2022年第19期10卷 6406-6416页

作者： Shuai-jie Lv Xiao-Jian Wang jie-feng huang Qiang Mao Bang-Jian He Pei-Jian Tong Department of Orthopedics and Traumatology The First Affiliated Hospital and First Clinical College of Zhejiang Chinese Medical UniversityHangzhou 310006Zhejiang ProvinceChina The First Clinical Medical School Zhejiang Chinese Medical UniversityHangzhou 310053Zhejiang ProvinceChina

BACKGROUND Nearly 10%of patients undergoing primary total knee arthroplasty(TKA)have valgus deformity(VD)of the *** severe VD of the knee,a more lateral structural release is needed to achieve balance between medial and lateral space and neutral femorotibial mechanical axis(FTMA),which is challenging and *** To introduce a new surgical technique of resection,soft tissue release,and FTMA for Ranawat type-Ⅱ VD with a 5-year *** A retrospective study was conducted on patients who underwent TKA from December 2011 to December ***-knee-ankle(HKA),range of motion(ROM),Oxford knee score(OKS),and knee society score(KSS)were used to assess the joint activity of patients in the new theory TKA group(NT-TKA)and were compared with those of the conventional TKA group(C-TKA).RESULTS A total of 103 people(103 knees)were included in this study,including 42 patients with an average follow-up period of 83 mo in the C-TKA group and 61 patients with an average follow-up period of 76 mo in the NT-TKA *** patients had constrained prosthesis,one had common peroneal nerve injury,and two had joint instability in the C-TKA group,but none of these occurred in the NT-TKA *** were significant statistical differences in constrained prosthesis usage and complications between the groups(P=0.002 and P=0.034,respectively).The KSS at 1 mo post-operation for the C-TKA and NT-TKA groups were 11.2±3.8 and 13.3±2.9,respectively,with a significant difference(P=0.007).However,the data of HKA,ROM,OKS KSS,and prosthesis survival rate were insignificant(P>0.05)in both the preoperative and follow-up *** Adopting 5°-7°valgus cut angle for VD and sacrificing 2°neutral FTMA for severe VD which cannot be completely corrected during TKA can reduce the need for soft tissue release,maintain early joint stability,reduce the use of constrained prostheses,and minimize postoperative complications.

关键词： Valgus,knee Arthroplasty Osteotomy Mechanical axis Bone and soft-tissue balance

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Diversity, Structure, and Marker-Trait Association Analysis of the Maize Recombinant Inbred Line Population

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Agricultural Sciences in China 2011年第7期10卷 975-986页

作者： CHEN Jing-tang HU Li-zong ZHU Li-ying GUO Jin-jie ZHAO Yong-feng huang Ya-qun College of Agronomy Agricultural University of Hebei/Hebei Sub-Center of Chinese National Maize Improvement Center/Northern China Key Laboratory for Crop Germplasm Resources Ministry of Education Baoding 007100 P.R.China

Association mapping has emerged as a new tool to elucidate complex quantitative trait loci in maize, but there are few reports about systematic association analysis for the specific SSR markers with agronomic traits of interest in China. We investigated the morphological and genetic diversity and population structure for 76 maize recombinant inbred lines, and then association analysis were further performed between 48 simple sequence repeat loci and 17 morphological traits, consisting of nine ear-related traits and eight other traits. The 48 SSR markers were screened out and further classified into two groups including a group of loci in regions harboring reported quantitative trait loci that affect ear shape and a group of markers distributing on the whole genome randomly. The result indicated that the population of recombinant inbred lines was structured, showing five subpopulations. Our association results revealed that there were 82, 59, and 40 significant associations detected by K-test, logistic regression, and both analysis, respectively. When the 17 traits were considered separately, the significant associations between Q-SSRs and E-traits were raised to 27.8%, whereas the other groups of combinations ranged between 2.3 and 6.3%. As the proportion of significant associations is higher among the Q-SSR subset of markers and the subset of traits related to ear shape than those for all of the other combinations, we conclude that this approach is valid for establishing true positive marker-trait relationships. Our results also demonstrated that association mapping could complement and enhance previous QTL information for marker-assisted selection.

关键词： maize ear shape association mapping recombinant inbred lines

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Ru doping triggering reconstruction of cobalt phosphide for coupling glycerol electrooxidation with seawater electrolysis

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Journal of Energy Chemistry 2025年第1期100卷 317-326页

作者： Binglu Deng jie Shen Jinxing Lu Chuqiang huang Zhuoyuan Chen feng Peng Yunpeng Liu School of Materials Science and Hydrogen Energy Foshan University School of Chemistry and Chemical Engineering Guangzhou University Guangdong Key Laboratory for Hydrogen Energy Technologies

Seawater electrolysis is a promising approach for sustainable energy without relying on precious ***,the large-scale seawater electrolysis is hindered by low catalytic efficiency and severe anode corrosion caused by the harmful *** contrast to the oxygen evolution reaction （OER）and chlorin ion oxidation reaction （ClOR）,glycerol oxidation reaction （GOR） is more thermodynamically and kinetically favorable ***,a Ru doping cobalt phosphide （Ru-CoP2） is proposed as a robust bifunctional electrocatalyst for seawater electrolysis and GOR,for the concurrent productions of hydrogen and value-added *** in situ and ex situ characterization analyses demonstrated that Ru doping featured in the dynamic reconstruction process from Ru-CoP2to Ru-CoOOH,accounting for the superior GOR *** coupling GOR with hydrogen evolution was realized by employing Ru-CoP2as both anode and cathode,requiring only a low voltage of 1.43 V at 100 mA cm-2,which was 250 m V lower than that in alkaline *** work guides the design of bifunctional electrocatalysts for energy-efficient seawater electrolysis coupled with biomass resource upcycling.

关键词： Anodes

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Assessment of bone mineral density and bone metabolism in young OSA men

Assessment of bone mineral density and bone metabolism in yo...

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中国睡眠研究会第十届全国学术年会

作者： jie-feng huang Qi-Chang Lin

＜正＞Background:Hypoxia during sleep in patients with obstructive sleep apnea （OSA）may have an effect on bone metabolism. Few data regarding evaluation of bone metabolism in young individuals diagnosed with OSA hav...

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The Hilbert expansion of the Boltzmann equation in the incompressible Euler level in a channel

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Science China Mathematics 2025年第1期68卷 39-88页

作者： Feimin huang Weiqiang Wang Yong Wang feng Xiao Academy of Mathematics and Systems Science Chinese Academy of Sciences School of Mathematical Sciences University of Chinese Academy of Sciences Key Laboratory of Computing and Stochastic Mathematics (Ministry of Education) School of Mathematics and StatisticsHunan Normal University

The study of the hydrodynamic limit of the Boltzmann equation with physical boundary is a challenging problem due to the appearance of the viscous and Knudsen boundary *** this paper,the hydrodynamic limit from the Boltzmann equation with the specular reflection boundary condition to the incompressible Euler equations in a channel is *** on the multi-scaled Hilbert expansion,the equations with boundary conditions and compatibility conditions for interior solutions,and viscous and Knudsen boundary layers are derived under different scaling,***,some uniform estimates for the interior solutions,and viscous and Knudsen boundary layers are *** the help of the L2-L∞framework and the uniform estimates obtained above,the solutions to the Boltzmann equation are constructed by the truncated Hilbert expansion with multiscales,and hence the hydrodynamic limit in the incompressible Euler level is justified.

关键词： Boltzmann equation incompressible Euler equations hydrodynamic limit Hilbert expansion specular reflection boundary condition viscous boundary layer Knudsen boundary layer

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Photoinduced radical sulfur dioxide insertion with asymmetric cyclization of alkenes: accessing β-chiral sulfones bearing S-stereocentric cyclic sulfinamides

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Science China Chemistry 2025年第1期68卷 257-263页

作者： Jiapian huang Chenxin Wang Xu Wang Xiaoyu Zhou Wei Xiao jie Wu School of Pharmaceutical and Chemical Engineering & Institute for Advanced Studies Taizhou University State Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences School of Chemistry and Chemical Engineering Henan Normal University

The generation of various β-chiral sulfones bearing chiral cyclic sulfinamides through photoredox-catalyzed sulfur dioxide insertion with asymmetric radical cyclization is described. This method features a broad substrate scope and excellent stereospecificity. Computational studies reveal that the dispersion interaction between sulfinyl group and CH2SO2Ph group plays a crucial role in the stereoselectivity control.

关键词： β-chiral sulfones chiral cyclic sulfinamides sulfur dioxide insertion asymmetric radical cyclization stereoselectivity

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Long noncoding RNA LINC01106 promotes lung adenocarcinoma progression via upregulation of autophagy

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Oncology Research 2025年第1期33卷 171-184页

作者： GENGYUN SUN YIPING ZHENG JIANfeng CAI jie GAO LIE DONG XIANGBIN ZHANG YINGHUI huang Department of Respiratory and Critical Care Medicine The First Affiliated Hospital of Anhui Medical UniversityHefei230022China Department of Respiratory and Critical Care Medicine Nanping First Hospital Affiliated to Fujian Medical UniversityNanping353006China

Background:Long noncoding RNA,LINC01106 exhibits high expression in lung adenocarcinoma(LUAD)tumor tissues,but its functional role and regulatory mechanism in LUAD cells remain ***:LINC01106 expression was analyzed in LUAD tissues and its functional impact on LUAD cells was *** cells were silenced with sh-LINC01106 and injected into nude mice to investigate tumor *** downstream transcription factors and molecular mechanism were determined using the Human transcription factor database(TFDB)database and Gene Expression Profiling Interactive Analysis(GEPIA)***,the impact of linc01106 on autophagy was analyzed by determining the expression of autophagy-related genes(ATGs)in LUAD ***:Our results showed that LINC01106 exhibited upregulation in both LUAD tissues and cell *** silencing of LINC01106 demonstrated a suppressive effect on tumorigenesis in a xenograft mouse model of ***,LINC01106 was found to recruit TATA-binding protein-associated factor 15(TAF15),an RNA-binding protein,thereby enhancing the mRNA stability of TEA domain transcription factor 4(TEAD4).In turn,TEAD4 served as a transcription factor that bound to the LINC01106 promoter and regulated its *** assays indicated that LINC01106 promoted autophagy in LUAD cells by upregulating the expression of autophagy-related genes(ATGs).The silencing of LINC01106 in LUAD cells inhibited autophagy,and cell proliferation,and promoted apoptosis,which all were effectively reversed by ATG5 ***:Overall,LINC01106,transcriptionally activated by TEAD4,interacts with TAF15 to promote the stability of TEAD4 and upregulates the expression of ATGs,promoting malignancy of LUAD cells.

关键词： LINC01106 TAF15 TEAD4 ATG5 Lung adenocarcinoma(LUAD) Non-small cell lung cancer(NSCLC)

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Validation of fatty liver index and hepatic steatosis index for screening of non-alcoholic fatty liver disease in adults with obstructive sleep apnea hypopnea syndrome

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Chinese Medical Journal 2019年第22期132卷 2670-2676页

作者： Li-Da Chen jie-feng huang Qing-Shi Chen Guo-Fu Lin Hui-Xue Zeng Xiao-Fen Lin Xue-Jun Lin Li Lin Qi-Chang Lin Department of Respiratory and Critical Care Medicine Zhangzhou Affiliated Hospital of Fujian Medical UniversityZhangzhouFujian 363000China Department of Respiratory and Critical Care Medicine The First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian 350005China 'Fujian Provincial Sleep-disordered Breathing Clinic Center FuzhouFujian 350005China The Second Affiliated Hospital of Fujian Medical University QuanzhouFujian 362000China Department of Cadre Ward Zhangzhou Affiliated Hospital of Fujian Medical UniversityZhangzhouFujian 363000China Department of Laboratory Medicine Zhangzhou Affiliated Hospital of Fujian Medical UniversityZhangzhouFujian 363000China

Background:Obstructive sleep apnea hypopnea syndrome(OSAHS)is a contributing factor for non-alcoholic fatty liver di:(NAFLD).Non-invasive algorithms including fatty liver index(FLI)and hepatic steatosis index(HSI)have been used as a screening test for NAFLD in epidemiologic *** aim of this study is to compare the diagnostic accuracy of FLI and HSI for NAFLD detection in adults with ***:We enrolled consecutive adult subjects who were newly diagnosed with OSAHS from March 2016 to January *** was diagnosed by *** accuracy and cut-off point of the FLI and HSI to detect NAFLD were assessed by analyzing the area under the receiver operating characteristic(AUROC)curve and the maximum Youden index analysis,***:The 326 subjects were diagnosed as NAFLD according to ultrasound findings,while 105 subjects who had normal abdominal ultrasonography were grouped as *** FLI and HSI values were significantly higher in patients with NAFLD compared with *** AUROC of FLI and HSI for predicting NAFLD was 0.802(95%confidence interval[CI]0.762-0.839)and 0.753(95%CI 0.710-0.793),*** AUROC of FLI was significantly higher than that of HSI(P=0.0383).The optimal cut-off value of FLI and HSI was 60(sensitivity 66%and specificity 80%)and 35(sensitivity 81%and specificity 60%),***:Both FLI and HSI can serve as screening tools for NAFLD in OSAHS *** FLI shows better performance in diagnosing NAFLD than HSI.

关键词： Obstructive sleep apnea hypopnea syndrome Fatty liver index Hepatic steatosis index Non-alcoholic fatty liver disease

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