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Journal of Genetics and Genomics 2018年第1期45卷 47-50页

作者： Chong Feng Xiaomin Wang Hui Shi Quanmei Yan min Zheng Jing Li Quanjun Zhang Yumin Qin Yougang Zhong jidong mi Liangxue Lai Beijing SINOGENE Biotechnology Co. Ltd Beijing 102200 China Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine Institutes of Biomedicine and Health Chinese Academy of Sciences Guangzhou 510530 China University of Chinese Academy of Sciences Beijing 100049 China College of Veterinary Medicine China Agricultural University Beijing 100193 China Jilin Provincial Key Laboratory of Animal Embryo Engineering College of Veterinary Medicine Jilin University Changchun 130062 China

Atherosclerotic cardiovascular disease is the leading cause of death in the world which is resulted from complex interactions among multiple genetic and environmental factors （WHO）. Athero- sclerosis is a chronic inflammatory disease characterized by accumulation of lipids in the arterial wall （Gofman and Lindgren, 1950）. Tremendous clinical and experimental efforts have been made to reveal the pathogenesis of the disease. Nevertheless, the mechanism of atherosclerosis is still unclear. A suitable animal model to study metabolic disorders and subsequent atherosclerosis is a necessity. The traditional method by feeding high fat diet to establish animal models of atherosclerosis disease is time- consuming and laborious, and in many circumstances, the pheno- types are not consistent among the individual models.

关键词： Generation of ApoE deficient dogs via combination of embryo injection of CRISPR/Cas9 with somatic cell nuclear transfer

A dog carrying mutations in AVP-NPII exhibits key features of central diabetes insipidus

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Journal of Genetics and Genomics 2023年第4期50卷 280-283页

作者： Huijuan Xu Haiyang Hao Shirui Wang Xueru Liu Wen Lyu Zhentao Zuo Yan Zhuo jidong mi Yong Q.Zhang Rui Tian Huijuan Zhu State Key Laboratory for Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China Beijing Sinogene Biotechnology Co.Ltd Beijing 100005China Department of Endocrinology Peking Union Medical College HospitalBeijing 100730China State Key Laboratory of Brain and Cognitive Science Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Hefei Comprehensive National Science Center Institute of Artificial IntelligenceHefeiAnhui 230088China

Central diabetes insipidus(CDI)is a rare disease characterized by the excretion of copious amounts of diluted urine(polyuria),excess water intake(polydipsia),and a rise in serum sodium concentration(hypernatremia)(Christ-Crain et al.,2019).The neuropeptide arginine-vasopressin(AVP)is synthesized as a preprohormone along with its carrier protein neurophysin II(NPII)in hypothalamic supraoptic(SON)and paraventricular(PVN)magnocellular neurons,stored in the posterior pituitary,and secreted into the circulation.

关键词： AVP diluted rare

Dogs lacking Apolipoprotein E show advanced atherosclerosis leading to apparent clinical complications

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Science China(Life Sciences) 2022年第7期65卷 1342-1356页

作者： Hui Zhao Jianping Zhao Di Wu Zhaolin Sun Yang Hua min Zheng Yumei Liu Qi Yang Xiahe Huang Yuan Li Yueshan Piao Yingchun Wang Sin Man Lam Huijuan Xu Guanghou Shui Yongjun Wang Haifeng Yao Liangxue Lai Zhuo Du jidong mi Enqi Liu Xunming Ji Yong Q.Zhang Key Laboratory of Molecular Developmental Biology and CAS Center for Excellence in Brain Science and Intelligence TechnologyInstitute ofGenetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Guangzhou Institutes of Biomedicine and Health Chinese Academy of SciencesGuangzhou 510530China Beijing Sinogene Biotechnology Co.Ltd Beijing 102200China Xuanwu Hospital Capital Medical UniversityBeijing 100053China TianTan Hospital Capital Medical UniversityBeijing 100070China Beijing Petsguard Animal Hospital Beijing 100192China Laboratory Animal Center Xi’an Jiaotong University Health Science CenterXi’an 710049China

Atherosclerotic cardiovascular disease resulting from dysregulated lipid metabolism is the leading cause of morbidity and mortality *** E(ApoE)plays a critical role in cholesterol *** in lipid-metabolizing proteins including ApoE in multiple model organisms such as mice and rats exhibiting elevated levels of cholesterol have been widely used for dissecting the pathology of atherosclerosis,but few of these animal models exhibit advanced atherosclerotic plaques leading to ischemia-induced clinical symptoms,limiting their use for translational *** we report hypercholesterolemia and severe atherosclerosis characterized by stenosis and occlusion of arteries,together with clinical manifestations of stroke and gangrene,in ApoE knockout dogs generated by CRISPR/Cas9 and cloned by somatic cell nuclear transfer ***,the hypercholesterolemia and atherosclerotic complications in F0 mutants are recapitulated in their *** the ApoE-associated atherosclerosis and clinical manifestations in mutant dogs are more similar to that in human patients compared with those in other animal models,these mutant dogs will be invaluable in developing and evaluating new therapies,including endovascular procedures,against atherosclerosis and related disorders.

关键词： ApoE atherosclerosis hypercholesterolemia gangrene stroke

北京日出东方凯宾斯基酒店弧形圆钢管柱混凝土顶升浇筑技术

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施工技术 2015年第6期44卷 14-17页

作者：田承行米继东路伟伟北京城建集团有限责任公司北京100088

结合北京日出东方凯宾斯基酒店工程重点分析了弧形圆钢管柱自密实混凝土浇筑施工过程中的特点以及混凝土浇筑过程中遇到的难点,详细介绍了自密实混凝土顶升浇筑过程中采取的施工工艺流程及注意事项,采用混凝土截止阀和钢管柱防振动措施... 详细信息

结合北京日出东方凯宾斯基酒店工程重点分析了弧形圆钢管柱自密实混凝土浇筑施工过程中的特点以及混凝土浇筑过程中遇到的难点,详细介绍了自密实混凝土顶升浇筑过程中采取的施工工艺流程及注意事项,采用混凝土截止阀和钢管柱防振动措施以及柱内防孔洞措施等,取得了不错的效果。

关键词：混凝土弧形圆钢管柱自密实混凝土施工技术

TIMP-1 Promotes Expression of MCP-1 and Macrophage migration by Inducing Fli-1 in Experimental Liver Fibrosis

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Journal of Clinical and Translational Hepatology 2024年第7期12卷 634-645页

作者： Xiaoli Huang Xiaofan Wang Yanhong Wang Shuangjun Shen Wei Chen Tianhui Liu Ping Wang Xu Fan Lin Liu jidong Jia min Cong Liver Research Center Beijing Friendship HospitalCapital Medical UniversityBeijingChina Beijing Key Laboratory of Translational Medicine in Liver Cirrhosis and National Clinical Research Center of Digestive Disease BeijingChina Dongying People’s Hospital DongyingShandongChina Experimental and Translational Research Center Beijing Friendship HospitalCapital Medical UniversityBeijingChina

Background and Aims:Tissue inhibitor of metalloproteinase-1(TIMP-1)plays a role in the excessive generation of extracellular matrix in liver *** study aimed to explore the pathways through which TIMP-1 controls monocyte chemoattractant protein-1(MCP-1)expression and promotes hepatic macrophage ***:Liver fibrosis was triggered through carbon tetrachloride,and an adenoassociated virus containing small interfering RNA targeting TIMP-1(siRNA-TIMP-1)was administered to both rats and *** assessed the extent of fibrosis and macrophage *** molecular mechanisms regulating macrophage recruitment by TIMP-1 were investigated through transwell migration assays,luciferase reporter assays,the use of pharmacological modulators,and an analysis of extracellular vesicles(EVs).Results:siRNA-TIMP-1 alleviated carbon tetrachloride-induced liver fibrosis,reducing macrophage migration and MCP-1 ***-culturing macrophages with hepatic stellate cells(HSCs)post-TIMP-1 downregulation inhibited macrophage *** siRNATIMP-1-treated HSCs,microRNA-145(miRNA-145)expression increased,while the expression of Friend leukemia virus integration-1(Fli-1)and MCP-1 was *** of Fli-1 led to decreased MCP-1 expression,whereas Fli-1 overexpression increased MCP-1 expression within *** with miRNA-145 mimics reduced the expression of both Fli-1 and MCP-1,while miRNA-145 inhibitors elevated the expression of both Fli-1 and MCP-1 in ***-145 bound directly to the 3'-UTR of Fli-1,and mi RNA-145-EN-riched EVs secreted by HSCs after TIMP-1 downregulation influenced macrophage ***:TIMP-1 induces Fli-1 expression through miRNA-145,subsequently increasing MCP-1 expression and macrophage ***-145-enriched EVs from HSCs can transmit biological information and magnify the function of TIMP-1.

关键词： TIMP-1 Fli-1 MCP-1 miRNA-145 Liver fibrosis Extracellular vesicles

北京日出东方凯宾斯基酒店弧形圆钢管柱安装技术

维普期刊数据库评论

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施工技术 2015年第6期44卷 9-13页

作者：苟金瑞米继东田承行陶福兵北京城建集团有限责任公司北京100088

北京日出东方凯宾斯基酒店工程结构形式为钢筋混凝土核心筒和钢框架混合结构体系,钢框架由钢管混凝土柱和钢梁结构构成,外围钢管柱沿立面呈弧形布置,截面形式为圆形,每层14~22根钢管柱不等。重点分析了弧形圆钢管柱安装的特点及难点,研... 详细信息

北京日出东方凯宾斯基酒店工程结构形式为钢筋混凝土核心筒和钢框架混合结构体系,钢框架由钢管混凝土柱和钢梁结构构成,外围钢管柱沿立面呈弧形布置,截面形式为圆形,每层14~22根钢管柱不等。重点分析了弧形圆钢管柱安装的特点及难点,研究采用先进的施工技术和工艺,有效地解决了施工过程中的各种难题,确保了工程进展顺利。

关键词：柱弧形圆钢管柱吊装安装

北京日出东方凯宾斯基酒店框架式无立柱隐框曲面玻璃幕墙安装技术

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施工技术 2015年第6期44卷 22-25页

作者：田承行苟金瑞米继东王三亮北京城建集团有限责任公司北京100088

结合北京日出东方凯宾斯基酒店幕墙工程,重点分析了曲面玻璃幕墙安装过程中因为建筑外形特点而产生的问题和施工难点,针对上述问题提出竖向龙骨采用拉弯龙骨和设计一种新型玻璃幕墙与主体结构连接转接件等合理化建议和措施,确定取消立... 详细信息

结合北京日出东方凯宾斯基酒店幕墙工程,重点分析了曲面玻璃幕墙安装过程中因为建筑外形特点而产生的问题和施工难点,针对上述问题提出竖向龙骨采用拉弯龙骨和设计一种新型玻璃幕墙与主体结构连接转接件等合理化建议和措施,确定取消立柱区别于规则框架式隐框曲面玻璃幕墙的安装方案,确保了本工程施工质量和工期。

关键词：装饰工程玻璃幕墙曲面玻璃幕墙隐框安装

三角玻璃拟合不规则闭合双曲面玻璃幕墙关键技术研究

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施工技术 2015年第6期44卷 18-21页

作者：米继东路伟伟田承行王三亮北京城建集团有限责任公司北京100088

结合北京日出东方凯宾斯基酒店幕墙工程重点分析了闭合双曲面玻璃幕墙的特点,阐述了安装施工过程中存在的问题和施工难点,针对上述问题,通过精心研究,提出了新型万向调节玻璃夹具和幕墙龙骨与主体结构连接可调转接件等合理化建议,确定... 详细信息

结合北京日出东方凯宾斯基酒店幕墙工程重点分析了闭合双曲面玻璃幕墙的特点,阐述了安装施工过程中存在的问题和施工难点,针对上述问题,通过精心研究,提出了新型万向调节玻璃夹具和幕墙龙骨与主体结构连接可调转接件等合理化建议,确定闭合双曲面玻璃幕墙安装关键技术和新施工方法,采用此施工方法保证了本工程的施工质量和工期。

关键词：装饰工程玻璃幕墙三角玻璃不规则闭合双曲面安装

基于差分光声光谱法的SF_(6)气体泄漏故障检测及预警

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微型电脑应用 2024年第3期40卷 149-153页

作者：石琳米丰曾季东刁一桐国网冀北电力有限公司检修分公司山西大同037000

针对复合气体绝缘金属封闭开关(HGIS)设备在运行过程中出现的故障诊断以及SF_(6)气体泄漏故障检测效率低等问题,设计一种基于差分光声光谱法的气体检测方法。该方法通过利用分子红外吸收光谱技术,实现PPT及PPB量级的气体检测功能,避免... 详细信息

针对复合气体绝缘金属封闭开关(HGIS)设备在运行过程中出现的故障诊断以及SF_(6)气体泄漏故障检测效率低等问题,设计一种基于差分光声光谱法的气体检测方法。该方法通过利用分子红外吸收光谱技术,实现PPT及PPB量级的气体检测功能,避免空气自身光声效应的干扰。同时,通过构建设备状态空间模型,基于粒子滤波算法对卡尔曼滤波法进行扩展,比较状态变量的实时测量值与估计值,得到残差值并对系统故障进行预警。此外,加入自适应阈值方法检测设备故障,避免模型误差以及噪声的干扰造成误报警。实验结果显示,系统故障检测和故障预警效果较好,检测精度高于94%。

关键词：故障检测运维管理状态空间模型粒子滤波算法变量残差

Computational design and simulation of the Mg-Cu system gradeddensity impactors for complex loading experiments

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Science China(Physics,Mechanics & Astronomy) 2011年第9期54卷 1710-1716页

作者： BAI JingSong TANG mi LUO GuoQiang YU jidong YUAN Shuai DAI ChengDa WU Qiang TAN Hua National Key Laboratory of Shock Wave and Detonation Physics (LSD) Institute of Fluid Physics China Academy of Engineering Physics Mianyang 621900 China State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan 430070 China State key laboratory of Explosion Science and Technology Beijing Institute of Technology Beijing 100081 China

Two types of Mg-Cu composition system graded density impactors used for complex loading （shock loading and quasi-isentropic compression） are designed by the elastic-plastic hydrodynamic method in this paper. mixtures of metal powders in the Mg-Cu system are cast into a series of 17 and 25 uniform compositions ranging from 100% Mg to 100% Cu. The graded den- sity impactors are launched to the stationary 10 Ixm aluminum film and 12 mm LiF window targets by a two-stage light-gas gun in the National Key Laboratory for Shock Wave and Detonation Physics Research, Institute of Fluid Physics, CAEP, and the resulting wave profiles are measured with the DISAR system. Hydrodynamic simulation results are perfectly consistent with the experiments. Our work in this paper will set up a foundation for further research of controllable loading/releasing routes and rate experiments in the future.

关键词： complex loading graded density impactor Mg-Cu system simulations