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CO2 Exchange and Chlorophyll Fluorescence of Phosphoenolpyruvate Carboxylase Transgenic Rice Pollen Lines

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Journal of Integrative Plant Biology 2006年第12期48卷 1431-1438页

作者： Li-Li Ling Hong-Hui Lin Ben-Hua Ji De-mao jiao Key Laboratory of Bio-resources and Eco-environment（Ministry of Education） College of Life Sciences Sichuan University Chengdu 610064 China Institute of Life Sciences Nantong University Nantong 510061 China Jiangsu Academy of Agricultural Sciences 84-401 Nanjing 210014 China

To elucidate the photosynthetic physiological characteristics and the physiological inherited traits of rice （Oryza sativa L.） hybrids and their parents, physiological indices of photosynthetic CO2 exchange and chlorophyll fluorescence parameters were measured in leaves of the maize phosphoenolpyruvate carboxylase （PEPC） transgenic rice as the male parent, sp. japonica rice cv. 9516 as the female parent, and the stable JAAS45 pollen line. The results revealed that the PEPC gene could be stably inherited and trans- ferred from the male parent to the JAAS45 pollen line. Moreover, the JAAS45 pollen line exhibited high levels of PEPC activity, manifesting higher saturated photosynthetic rates, photosynthetic apparent quantum yield （AQY）, photochemical efficiency of photosystem II and photochemical and non-photochemical quenching, which indicated that the JAAS45 pollen line has a high tolerance to photo-inhibition/photooxidation under strong light and high temperature. Furthermore, JAAS45 was confirmed to still be a C3 plant by δ^13C carbon isotope determination and was demonstrated to have a limited photosynthetic C4 microcycle by feeding with exogenous C4 primary products, such as oxaloacetate or malate, or phosphoenolpyruvate. The present study explains the physiological inherited properties of PEPC transgenic rice and provides an expectation for the integration of traditional breeding and biological technology.

关键词： anther culture phosphoenolpyruvate carboxylase (PEPC) physiological inherited trait rice hybrid transgenic rice

Capacitance of Organic Schottky Diodes Based on Copper Phthalocyanine （CuPc）

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Chinese Physics Letters 2010年第6期27卷 175-178页

作者： LI Zhong-Liang WU Zhao-Xin jiao Bo mao Gui-Lin HOU Xun Key Laboratory of Photonics Technology for Information (Shaanxi Province) and Key Laboratory for Physical Electronics and Devices of the Ministry of Education School of Electronic and Information Engineering Xi'an Jiaotong University Xi'an 710049

The capacitance of an organic Schottky diode based on copper phthalocyanine （CuPc） is investigated. Based on the organic small-signal equivalent model established, we calculate the reverse capacitance CMetal Of the organic Schottky diode with different kinds of metal cathodes （Mg, Al, Au）. It is found that the reverse capacitance of the organic Schottky diode shows behavior as CMg 〉 CAl 〉 CAu at the same frequency, and according to our analysis, the reverse Schottky junction capacitance Cj is expected to have little effect on the reverse capacitance of the organic Schottky diode, and the space-charge limited current capacitance Us is considered to dominate the reverse capacitance, which limits the improvement of frequency characteristics of organic Schottky diodes.

关键词： Organizations Beers Metals Aluminium Magnesium

Relationships between Phosphatidylglycerol Molecular Species of Thylakoid Membrane Lipids and Sensitivities to Chilling-induced Photoinhibition in Rice

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Journal of Integrative Plant Biology 2008年第2期50卷 194-202页

作者： Su-Qin Zhu Hua Zhao Jian-Sheng Liang Ben-Hua Ji De-mao jiao College of Bioscience and Biotechnology Yangzhou University Yangzhou 225000 China Institute of Bioengineering Nantong University Nantong 226007 China College of Life Sciences Nantong University Nantong 226007 China Institute of Agrobiological Genetics and Physiology Jiangsu Academy of Agricultural Sciences N anjing 210014 China

In an attempt to explore the relationships between phosphatidylglycerol （PG） molecular species of thylakoid membrane lipids and sensitivities to chilling-induced photoinhibition, PG molecular species, D1 protein, electron transport activities of thylakoid membrane and the potential quantum yield （FvlFm） in rice treated under middle and low photon flux density （PFD） at 11℃ were analyzed by high performance liquid chromatography, enzyme hydrolysis, gas phase chromatography （GC） and so on. Results showed that the major molecular species of PGs in rice thylakoid membrane were 18：3/16：0, 18：3/16：1（3t）, 18：2/16：0, 18：2/16：1（3t）, 18：1/16：0, 18：1/16：1（3t）, 16：0/16：0, 16：0/16：1（3t）. There were large differences in the contents of unsaturated PG molecular species such as 18：1-3/16：0-16：1（3t） and saturated PG molecular species like 16：0/16：0-16：1（3t） among japonica cv 9516 0-9516）, japonica-indica hybrid F1 j-9516/i-SY63 （ji-95SY） and indica cv Shanyou 63 （i-SY63）. J-9516 containing higher contents of unsaturated PG molecular species was manifest in stable D1 protein contents under chill and tolerant to chill-induced photoinhibition. In contrast to j-9516, i-SY63 with lower contents of unsaturated PG molecular species, exhibited unstable D1 protein contents under chill and was sensitive to chill-induced photoinhibition, ji-95SY containing middle contents of unsaturated PG molecular species between those of j-9516 and i-SY63, exhibited mid extent of sensitivity to chill-induced photoinhibition. The losses in D1 protein also account for the inhibition in electron transport activity of thylakoid membrane and the observed decline in FvlFm. The PG molecular species that is efficient in raising chilling-resistant capacity were those containing unsaturated fatty acids, namely, unsaturated PG molecular species. These results implied that the substrate selectivity of the glycerol-3-phosphate acyltransferase in chloroplasts towards 16：0 or 18：1 displayed greatly the difference between j

关键词： chilling-induced photoinhibition D1 protein molecular species phosphatidylglycerol rice.

Changes in Unsaturated Levels of Fatty Acids in Thylakoid PSII Membrane Lipids During Chilling-induced Resistance in Rice

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Journal of Integrative Plant Biology 2007年第4期49卷 463-471页

作者： Su-Qin Zhu Chun-Mei Yu Xin-Yan Liu Ben-Hua Ji De-mao jiao Institute of Bioengineering Nantong University Nantong 226007 China College of Life Sciences Nantong University Nantong 226007 China Institute of Agrobiological Genetics and Physiology Jiangsu Academy of Agricultural Sciences Nanjing 210014 China

Temperature is one of the abiotic factors limiting growth and productivity of plants. In the present work, the effect of low non-freezing temperature, as an inducer of ＂chilling resistance＂, was studied in three cultivars of rice （Oryza sativa L.）, japonica cv. 9516 （j-9516）, the two parental lines of superhigh-yield hybrid rice between subspecies, Peiai/E32 （ji-PE）, and the traditional indica hybrid rice Shanyou 63 （i-SY63）. Leaves of chill-treated rice showed chilling-induced resistance, as an increase of their low-temperature tolerance was measured using chlorophyll fluorescence measurements, revealing a change in photosystem II （PSII） efficiency. After 5 d of exposure to 11 ~C under low light （100 pmol·m^-2·s^-1）, levels of unsaturated fatty acids in PSII thylakoid membrane lipids decreased during the initial 1-2 d, then increased slowly and reached 99.2%, 95.3% and 90.1% of the initial value （0 d） in j-9516, ji-PE and i-SY63, respectively, on the fifth day. However, under medium light （600 μmol·m^-2·s^-1）, all cultivars experienced similar substantial photoinhibition, which approached steady state levels after a decline in levels of unsaturated fatty acids in PSII thylakoid membrane lipids to about 57.1%, 53.8% and 44.5% of the initial values （0 d） in j-9516, ji-PE and i-SY63 on the fifth day. Under either chilling-induced resistance （the former） or low temperature photoinhibition （the latter） conditions, the changes of other physiological parameters such as D1 protein contents, electron transport activities of PSII （ETA）, Fv/Fm, xanthophyl cycle activities expressed by DES （deepoxide state） were consistent with that of levels of unsaturated fatty acids in PSII thylakoid membrane lipids. So there were negative correlations between saturated levels of fatty acids （16：1（3t）, 16：0, 18：0）, especially the 16：1（3t） fatty acid on thylakoid membrane and other physiological parameters, such as D1 protein contents, ETA and （A＋Z）/（A＋V＋Z）. A specific role of desaturation o

关键词： chilling-induced resistance D1 protein rice thylakoid PSII membrane lipids unsaturated fatty acids xanthophyl cycleactivity.

Response of Gas Exchange and Water Use Efficiency to Light Intensity and Temperature in Transgenic Rice Expressing PEPC and PPDK Genes

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Agricultural Sciences in China 2009年第11期8卷 1312-1320页

作者： ZHANG Bian-jiang CHEN Quan-zhan HUA Chun ZHOU Feng ZHOU Quan-chen jiao De-mao Nanjing Xiaozhuang University Nanjing 211171 P.R. China Jiangsu Academy of Agricultural Sciences Nanjing 210014 P.R. China

Aiming to controvert whether the photosynthetic capacity of transgenic rice expressing C4 genes is enhanced, with the C3-type untransformed rice （WT） and maize （a C4 plant） as controls, the activity of C4 photosynthetic enzymes, gas exchange parameters and water use efficiency （WUE） under different light intensities and temperatures, the stable carbon isotope ratio （8-3C） value and the metabolic index of active oxygen as well as plant yield parameters were determined in transgenic rice carrying the PEPC and PPDK genes （CK） in this study. The results showed that the light-saturated photosynthetic rate of CK was intermediate between that of WT and maize, with a slight bias towards that of maize. Under a high light intensity （1 200 μmol m^-2 s^-1） and high temperature （35℃）, CK still exhibited higher photosynthetic capacity, while the Gs decreased. The WUE of CK was only slightly increased, and was similar to that of WT. The δ13C value indicated that CK functioned as a C3 plant. In addition, the tolerance to photo-oxidation and grain yield of CK was enhanced by sprayed with NaHSO3. In conclusion, CK possesses higher photosynthetic productivity under the conditions of high photon flux density （PFD）, high temperature and spraying with NaHSO3 solution, thereby providing a new technical approach and physiological basis for constructing C4-like rice.

关键词： transgenic rice photosynthetic characteristics water use efficiency stomatal conductance

The Impact of Varied Fertilizers on the Yield and Quality of Panicum miliaceum under Low Nitrogen Conditions

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Phyton-International Journal of Experimental Botany 2024年第10期93卷 2629-2644页

作者： Mengyao Wang jiao mao Yuanmeng Xu Shu Wang Yuhan Liu Jiangling Ren Sichen Liu Zhijun Qiao Xiaoning Cao Center for Agricultural Genetic Resources Research Shanxi Agricultural UniversityTaiyuan030031China College of Agriculture Shanxi Agricultural UniversityJinzhong030801China Key Laboratory of Crop Genetic Resources and Germplasm Development in Loess Plateau Ministry of Agriculture and Rural AffairsTaiyuan030031China

To clarify the response characteristics of broomcorn millet yield and quality to various fertilizers under low nitrogen conditions,the present study investigated the effects of different fertilization treatments,including no fertilization(HCK,CCK),urea(HF1,CF1),phosphate fertilizer(HF2,CF2),compound fertilizer(HF3,CF3),and organic fertilizer(HF4,CF4),on the agronomic traits,quality,and starch pasting properties of proso *** experimental materials,non-waxy proso millet(Hequ red proso millet,denoted as HQH)and waxy proso millet(Chishu 1,denoted as CS1)were *** results showed that under low nitrogen conditions,urea treatment(HF1)significantly increased plant height and yield,and phosphate fertilizer treatment(HF2)significantly increased thousand-grain weight(TW).For waxy proso millet Chishu NO.1(CS1),urea treatment(CF1)significantly increased yield,phosphate fertilizer treatment(CF2)significantly influenced the height of the plants,and compound fertilizer treatment(CF3)significantly increased thousand-grain *** terms of quality,phosphate fertilizer treatment(CF2)and organic fertilizer treatment(CF4)resulted in a substantial augmentation of both the total starch content and amylose content of waxy proso millet Chishu NO.1(CS1),respectively,and organic fertilizer treatment(HF4,CF4)resulted in a notable enhancement of the protein content of proso *** fertilizer treatment(HF4)increased peak viscosity(PV),hot viscosity(HV),setback viscosity(SV),final viscosity(FV),breakdown viscosity(BV),pasting time(PTim),and pasting temperature(PTemp)of non-waxy proso *** fertilizer treatment(CF2)increased the peak viscosity(PV),hot viscosity(HV),breakdown viscosity(BV),final viscosity(FV),pasting time(PTim),and pasting temperature(PTemp)of waxy proso *** fertilizer treatment(HF4)and phosphate fertilizer treatment(CF2)under low nitrogen were the most effective in improving the comprehensive quality of non-waxy and waxy proso millet,r

关键词： Proso millet low nitrogen yield quality

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基础隔震结构屋面突出物的水平地震作用研究

振动与冲击 2010年第10期29卷 182-186页

作者：焦春节焦春茂同济大学建筑工程系上海200092 广东省电力设计研究院广州510663

根据《建筑抗震设计规范》(GB50011-2001)和《叠层橡胶支座隔震技术规程》(CECS126:2001),在理论分析基础上,得到采用等效侧力法时屋面突出物水平地震作用修正系数βn的表达式。指出βn的控制因素为突出物与主体结构的质量比α和突出物... 详细信息

根据《建筑抗震设计规范》(GB50011-2001)和《叠层橡胶支座隔震技术规程》(CECS126:2001),在理论分析基础上,得到采用等效侧力法时屋面突出物水平地震作用修正系数βn的表达式。指出βn的控制因素为突出物与主体结构的质量比α和突出物和隔震层间刚度比β。并仿照《高层建筑混凝土结构技术规程》(JGJ3-2002),得到基础隔震结构屋面突出物水平地震作用修正系数βn的实用表格。同时基于有限元理论的振型分解反应谱法建立模型计算,给出了修正系数。通过算例分析,可知结果可供工程中使用。

关键词：屋面突出物修正系数隔震结构等效侧力法水平地震作用

Advances in mesenchymal stem cells therapy for tendinopathies

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Chinese Journal of Traumatology 2024年第1期27卷 11-17页

作者： Xu-Feng mao Xi-Qian Zhang Zhe-Yu Yao Hai-jiao mao Department of Orthopaedic Surgery The First Affiliated Hospital of Ningbo UniversityNingbo315010Zhejiang provinceChina

Tendinopathies are chronic diseases of an unknown etiology and associated with *** stem cells(MSCs)have emerged as a viable therapeutic option to combat the pathological progression of tendinopathies,not only because of their potential for multidirectional differentiation and self-renewal,but also their excellent immunomodulatory *** immunomodulatory effects of MSCs are increasingly being recognized as playing a crucial role in the treatment of tendinopathies,with MSCs being pivotal in regulating the inflammatory microenvironment by modulating the immune response,ultimately contributing to improved tissue *** review will discuss the current knowledge regarding the application of MSCs in tendinopathy treatments through the modulation of the immune response.

关键词： Mesenchymal stem cells Tendinopathy Immunomodulation Treatment Review

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基于协同注意力的小样本的手机屏幕缺陷分割

电子与信息学报 2022年第4期44卷 1476-1483页

作者：许国良毛骄重庆邮电大学通信与信息工程学院重庆400065 重庆邮电大学电子信息与网络工程研究院重庆400065

在手机屏幕工业化生产过程中,缺陷检测的好坏直接影响手机屏幕的合格率。少量的缺陷样本不足以完成数据驱动的分割网络的训练,因此如何利用少量的缺陷图像完成缺陷分割成为关键问题。该文针对此问题提出一种基于协同注意力的小样本手机... 详细信息

在手机屏幕工业化生产过程中,缺陷检测的好坏直接影响手机屏幕的合格率。少量的缺陷样本不足以完成数据驱动的分割网络的训练,因此如何利用少量的缺陷图像完成缺陷分割成为关键问题。该文针对此问题提出一种基于协同注意力的小样本手机屏幕缺陷分割网络(Co-ASNet)。该网络使用交叉注意力块在特征提取时获取更加丰富的上下文缺陷特征信息,同时引入了协同注意力的方式来加强支持图像与查询图像相同缺陷目标之间的特征信息交互,增强缺陷特征表示,另外,使用了改进的联合损失函数来完成网络的训练。该文采用手机屏幕缺陷数据集进行实验,实验结果表明,Co-ASNet能够使用少量的缺陷样本完成良好的缺陷分割效果。

关键词：手机屏幕缺陷小样本分割协同注意力交叉注意力