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Aquaculture and Fisheries 2023年第1期8卷 33-41页

作者： Xulu Chang Meiru kang Junchang Feng jianxin Zhang Xianfeng Wang College of Fisheries Henan Normal UniversityXinxiang453007China

Polybrominated diphenyl ether has become a widespread environmental pollutant in aquatic ***-209,a prominent PBDEs found in the environment and wildlife,has been proven to cause oxidative stress,immune damage and endocrine system disorders in ***,the effects of BDE-209 on the intestinal health of fish remain *** research aimed to determine the effects of BDE-209 on the growth performance,digestive enzymes activities and intestinal microbiota of common *** 60-day exposure,the growth performance of common carp were remarkably decreased(P0.05).Overall,exposure to BDE-209 could alter the intestinal microbiota of common carp,which may affect their growth and threaten their health.

关键词： BDE-209 Cyprinus carpio intestinal Microbiota Digestive enzyme

A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification

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Journal of Animal Science and Biotechnology 2015年第1期6卷 22-28页

作者： kang kang Keli Yang Jiasheng Zhong Yongxiang Tian Limin Zhang jianxin Zhai Li Zhang Changxu Song Christine Yuan Gou Jun Luo Deming Gou College of Animal Science and Technology Northwest A&F University College of Life Sciences Shenzhen Key Laboratory of Microbial Genetic EngineeringShenzhen University Hubei Key Laboratory of Animal Embryo and Molecular Breeding Institute of Animal Husbandry and VeterinaryHubei Academy of Agricultural Sciences Shenzhen Ao Dong Inspection and Testing Technology Co .Ltd Veterinary Medicine Institute Guangdong Academy of Agricultural Sciences Northwestern University

Background： Porcine reproductive and respiratory syndrome virus （PRRSV）, and particularly its highly pathogenic genotype （HP-PRRSV）, have caused massive economic losses to the global swine industry. Results： To rapidly identify HP-PRRSV, we developed a direct reaL-time reverse transcription polymerase chain reaction method （dRT-PCR） that could detect the virus from serum specimen without the need of RNA purification Our dRT-PCR assay can be completed in 1.5 h from when a sample is received to obtaining a result. Additionally, the sensitivity of dRT-PCR matched that of conventional reverse transcription PCR （cRT-PCR） that used purified RNA The lowest detection limit of HP-PRRSV was 6.3 TCIDs0 using dRT-PCR. We applied dRT-PCR assay to 144 field samples and the results showed strong consistency with those obtained by cRT-PCR. Moreover, the dRT-PCR method was able to tolerate 5-20% （v/v） serum. Conclusions： Our dRT-PCR assay allows for easier, faster, more cost-effective and higher throughput detection of HP-PRRSV compared with cRT-PCR methods. To the best of our knowledge, this is the first report to describe a real-time RT-PCR assay capable of detecting PRRSV in crude serum samples without the requirement for purifying RNA. We believe our approach has a great potential for application to other RNA viruses.

关键词： Highly pathogenic Porcine reproductive and respiratory syndrome virus Real-time RT-PCR

Optimized CeO_(2) Nanowires with Rich Surface Oxygen Vacancies Enable Fast Li-Ion Conduction in Composite Polymer Electrolytes

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Energy & Environmental Materials 2023年第1期6卷 218-223页

作者： Lu Gao Nan Wu Nanping Deng Zhenchao Li jianxin Li Yong Che Bowen Cheng Weimin kang Ruiping Liu Yutao Li State Key Laboratory of Separation Membranes and Membrane Processes/National Center for International Joint Research on Separation Membranes School of Textile Science and EngineeringTiangong UniversityTianjin 300387China Materials Science and Engineering Program and Texas Materials Institute The University of Texas at AustinAustin TX 78712USA Department of Materials of Science and Engineering China University of Mining and Technology(Beijing)Beijing 100083China Enpower Energy Corp. Beijing 102600China

Low-cost and flexible solid polymer electrolytes are promising in all-solid-state Li-metal batteries with high energy density and ***,both the low room-temperature ionic conductivities and the small Li^(+)transference number of these electrolytes significantly increase the internal resistance and overpotential of the ***,we introduce Gd-doped CeO_(2) nanowires with large surface area and rich surface oxygen vacancies to the polymer electrolyte to increase the interaction between Gd-doped CeO_(2) nanowires and polymer electrolytes,which promotes the Li-salt dissociation and increases the concentration of mobile Li ions in the composite polymer *** optimized composite polymer electrolyte has a high Li-ion conductivity of 5×10^(-4)4 S cm^(-1) at 30℃ and a large Li+transference number of ***,the composite polymer electrolytes have excellent compatibility with the metallic lithium anode and high-voltage LiNi_(0.8)Mn _(0.1)Co_(0.1)O_(2)(NMC)cathode,providing the stable cycling of all-solid-state batteries at high current densities.

关键词： composite polymer electrolytes,Gd-doped CeO_(2)nanowires Li-ion conduction oxygen vacancies surface interaction

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湖南壶天石羊肌肉营养成分研究

黑龙江畜牧兽医 2024年第11期 42-46,56页

作者：杨龙林小丁黄鹏吴买生张兴黄珂康萌陈钰焓彭佩雅陈月华钱建新刘梅湖南农业大学动物科学技术学院长沙410128 湘潭市农业农村局湖南湘潭411104 湘乡市畜牧水产事务中心湖南湘潭411104

为了分析湖南壶天石羊的肌肉营养成分,并比较分析壶天石羊在不同年龄阶段(6,12,24月龄)的肌肉差异特征,试验选择纯种的24月龄壶天石羊母羊12只、12月龄壶天石羊母羊6只,屠宰后取背最长肌测定常规营养成分及矿物质、氨基酸、脂肪酸含量,... 详细信息

为了分析湖南壶天石羊的肌肉营养成分,并比较分析壶天石羊在不同年龄阶段(6,12,24月龄)的肌肉差异特征,试验选择纯种的24月龄壶天石羊母羊12只、12月龄壶天石羊母羊6只,屠宰后取背最长肌测定常规营养成分及矿物质、氨基酸、脂肪酸含量,并与已报道的6月龄壶天石羊背最长肌相应指标数据进行比较分析。结果表明:24月龄壶天石羊与12月龄壶天石羊相比,水分、粗蛋白、粗脂肪、粗灰分和干物质均差异不显著(P>0.05);与6月龄壶天石羊相比,24月龄壶天石羊背最长肌中粗脂肪含量升高,粗蛋白含量降低。与12月龄壶天石羊相比,24月龄壶天石羊背最长肌中镁含量极显著升高(P<0.01),锌含量也明显增加(P=0.06);24月龄壶天石羊锌含量明显高于6月龄壶天石羊,增加量超过120%;24月龄壶天石羊的铜残留量(1.46μg/g)高于6月龄壶天石羊,高出71.76%,铅、锰残留量则非常低。与12月龄壶天石羊相比,24月龄壶天石羊背最长肌中天冬氨酸、谷氨酸、丝氨酸、甘氨酸、苏氨酸、精氨酸、赖氨酸、亮氨酸、丙氨酸、酪氨酸、缬氨酸、异亮氨酸、半胱氨酸、甲硫氨酸含量均增加;24月龄壶天石羊背最长肌中天冬氨酸、苏氨酸、赖氨酸、亮氨酸、丙氨酸、缬氨酸、异亮氨酸含量相比于6月龄增幅均超过30.00%。24月龄壶天石羊背最长肌中必需氨基酸和呈味氨基酸含量分别为9.02%、16.24%,均高于6,12月龄壶天石羊。与6月龄壶天石羊相比,24月龄壶天石羊肌肉中含有较多的天冬氨酸、必需氨基酸、呈味氨基酸、十七碳酸、十七烷酸和花生一烯酸,而含有较低的硬脂酸、花生四烯酸、α-亚麻酸和亚油酸。说明壶天石羊在不同年龄段的氨基酸成分和肌内脂肪含量等营养成分具有明显差异,并具有优良肉质特性。

关键词：壶天石羊年龄肌肉成分营养成分肌内脂肪

基于地理探测器和GIS的云南省传统村落空间分布格局及其影响因素研究

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云南师范大学学报（自然科学版） 2024年第2期44卷 69-78页

作者：郭帅龙杨建欣马长乐龚郁舒高灿康新玲李瑞西南林业大学园林园艺学院云南昆明650224 国家林业和草原局西南风景园林工程技术研究中心云南昆明650224

以云南省777个传统村落为研究对象,使用Arc GIS10.7工具,综合采用空间自相关、最邻近指数、标准差椭圆、核密度分析和地理探测器等多种方法,从自然、社会和文化三大维度探析云南省传统村落的空间分布特征及其影响因素.结果表明:(1)云南... 详细信息

以云南省777个传统村落为研究对象,使用Arc GIS10.7工具,综合采用空间自相关、最邻近指数、标准差椭圆、核密度分析和地理探测器等多种方法,从自然、社会和文化三大维度探析云南省传统村落的空间分布特征及其影响因素.结果表明:(1)云南省传统村落空间分布上整体呈现出“滇西密、滇东疏、西北密、东北疏、东南密、西南疏”的分布格局.(2)云南省传统村落空间分布具有显著的指向性和集聚性,全省整体呈现出“三核状”分布格局,表现为集聚与分散相结合的分布形态.其中整体及局部指向性均为“西北至东南指向”.空间分布集聚性上,高密度区主要分布于红河哈尼族彝族自治州(滇南)、保山市(滇西)、大理白族自治州和丽江市(滇西北).(3)自然、人文、社会和政策导向等因素的相互耦合作用形成了云南省传统村落的空间分布特征.水资源储量、人均GDP、道路通车里程、少数民族占比与文化多样性指数等是主要的影响因素.

关键词：传统村落空间分布地理探测器 Arc GIS 影响因素云南省

Effect of tetramethylpyrazine on the spatial learning and memory function of rats after focal cerebral ischemia

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Neural Regeneration Research 2006年第2期1卷 161-165页

作者： Jianjun Zhao Yong Liu Xinlin Chen jianxin Liu Yingfang Tian Pengbo Zhang Qianyan kang Fen Qiu Department of Human Anatomyand Histological Embryology School of Medicine Xi＇an Jiaotong University Xi＇an 710061Shaanxi Province. China

BACKGROUND： Tetramethylpyrazine （TMP） presents the effect of anti-platelet aggregation, reduces arteria resistance, increases cerebral blood flow, and improves microcirculation. OBJECTIVE： To observe the effects of TMP on the learning and memory abilities and the number of neurons in cortex and hippocampus after focal cerebral ischemia in rats DESIGN： A randomized controlled tria SETTING： Department of Human Anatomy and Histological Embryology, School of Medicine, Xi＇an Jiaotong University. MATERIALS： Fifty adult male Sprague-Dawley rats, weighing 250-300 g were supplied by the Experimental Animal Center, School of Medicine, Xi＇an Jiaotong University. TMP was purchased from Wuxi Seventh Pharmaceutical *** （Lot Number： 2004051106, Specification： 2 mL/piece）. METHODS ： The experiments were carried out in School of Medicine of Xi＇an Jiaotong University from June 2004 to May 2005. The 50 rats were randomly divided into five groups according to the random number table method： sham-operated group, cerebral ischemia control group, low-dose TMP group, middle-dose TMP group and high-dose TMP group, 10 rats in each group. Rats in the TMP groups were immediately treated with intraperitoneal injection of TMP of 40, 80 and 120 mg/kg respectively, and those in the sham-operated group and cerebral ischemia control group were injected intraperitoneally by isovolume saline, once a day for 14 days successively. On the 15^th day, the spatial learning and memory abilities of the rats were assessed with the Morris water maze test, and then the changes of neuron numbers in cortex and hippocampus were observed by Nissl staining of brain sections. MAIN OUTCOME MEASURES ： The results of Morris water maze test and the changes of neuron numbers in cortex and hippocampus by Nissl staining of brain sections were observed. RESULTS： Finally 39 rats were involved in the analysis of results, and the other 11 died of excessive anesthesia or failure in model establishment. ① The rats

关键词： Effect of tetramethylpyrazine on the spatial learning and memory function of rats after focal cerebral ischemia TM high show

Surface reconstruction establishing Mott-Schottky heterojunction and built-in space-charging effect accelerating oxygen evolution reaction

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Nano Research 2022年第4期15卷 2952-2960页

作者： Yao kang Shuo Wang Kwan San Hui Shuxing Wu Duc Anh Dinh Xi Fan Feng Bin Fuming Chen jianxin Geng Weng-Chon(Max)Cheong Kwun Nam Hui Joint Key Laboratory of the Ministry of Education Institute of Applied Physics and Materials EngineeringUniversity of MacaoTaipaMacao 999078China Engineering Faculty of ScienceUniversity of East AngliaNorwich NR47TJUK School of Chemical Engineering and Light Industry Guangdong University of TechnologyGuangzhou 510006China NTT hi-tech institute Nguyen Tat Thanh universityHo Chi Minh city 700000Vietnam Ningbo Institute of Materials Technology EngineeringChinese Academy of SciencesNingbo 315201China State Key Laboratory of High-Temperature Gas Dynamics Institute of MechanicsChinese Academy of ScienceBeijing 100190China State Key Laboratory of Optic Information Physics and Technologies School of Physics and Telecommunication EngineeringSouth China Normal UniversityGuangzhou 510006China State Key Laboratory of Organic-Inorganic Composites Beijing University of Chemical Technology15 North Third Ring East RoadChaoyang DistrictBeijing 100029China Department of Physics and Chemistry Faculty of Science and TechnologyUniversity of MacaoTaipaMacao 999078China

Structural reconstruction of nanomaterials offers a fantastic way to regulate the electronic structure of active sites and promote their catalytic ***,how to properly facilitate surface reconstruction to overcome large overpotential that stimulate the surface reconstruction has remained ***,we adopt a facile approach to activate surface reconstruction on Ni(OH)_(2) by incorporating F anions to achieve electro-derived structural oxidation process and further boost its oxygen evolution reaction(OER)*** situ Raman and X-ray photoemission spectroscopy studies indicate that F ions incorporation facilitated surface reconstruction and promotes the original Ni(OH)_(2)transformed into a mesoporous and amorphous F-NiOOH layer during the electrochemical *** functional theory(DFT)calculation reveals that this self-reconstructed NiOOH induces a space-charge effect on the p-n junction interface,which not only promotes the absorption of intermediates species(^(*)OH,^(*)O,and^(*)OOH)and charge-transfer process during catalysis,but also leads to a strong interaction of the p-n junction interface to stabilize the *** work opens up a new possibility to regulate the electronic structure of active sites and promote their catalytic activities.

关键词： F anions dynamic migration nickel hydroxides surface reconstruction oxygen evolution reaction(OER)

Dynamics of a qubit—TLS system under resonant microwave driving

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Chinese Science Bulletin 2014年第21期59卷 2547-2551页

作者： jianxin Shi Weiwei Xu Guozhu Sun Jiquan Zhai Zhican Du Lin Wang Tao Hua Jian Chen Lin kang Peiheng Wu Research Institute of Superconductor Electronics (RISE) School of Electronic Science and EngineeringNanjing University

We demonstrate the effect of different coupling strengths between a microscopic two-level system(TLS)and a microwave field on the dynamics of a qubit—TLS system when the bipartite system is subject to resonant microwave *** beating with a different TLSmicrowave coupling strength is demonstrated in ***,quantified by the concurrence between the qubit and TLS,both for pure states and mixed states,is *** decoherence is considered,entanglement of the bipartite system oscillates with damping and exhibits entanglement sudden death and/or entanglement sudden death and revival.

关键词： TLS 微波场系统驾驶共振量子位耦合强度突然死亡

Soliton microcomb-assisted microring photonic thermometer with ultra-high resolution and broad range

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Photonics Research 2023年第10期11卷 I0001-I0010页

作者： CHENG ZHANG JIN WANG GUOGUO kang jianxin GAO ZHIER QU SHUAI WAN CHUNHUA DONG YIJIE PAN JIFENG QU Center for Advanced Measurement Science National Institute of MetrologyBeijing 100029China School of Optics and Photonics Beijing Institute of TechnologyBeijing 100081China College of Optical and Electronic Technology China Jiliang UniversityHangzhou 310018China CAS Key Laboratory of Quantum Information University of Science and Technology of ChinaHefei 230026China CAS Center for Excellence in Quantum Information and Quantum Physics University of Science and Technology of ChinaHefei 230026China

Whispering gallery mode resonators(WGMRs)have proven their advantages in terms of sensitivity and precision in various sensing ***,when high precision is pursued,the WGMR demands a high-quality factor usually at the cost of its free spectral range(FSR)and corresponding measurement *** this article,we propose a high-resolution and wide-range temperature sensor based on chip-scale WGMRs,which utilizes a Si_(3)N_(4)ring resonator as the sensing element and a Mg F_(2)-based microcomb as a broadband frequency *** measuring the beatnote signal of the WGM and microcomb,the ultra-high resolution of 58 micro-Kelvin(μK)was *** ensure high resolution and broad range simultaneously,we propose an ambiguity-resolving method based on the gradient of feedback voltage and combine it with a frequency-locking *** a proof-of-concept experiment,a wide measurement range of 45 K was *** soliton comb-assisted temperature measurement method offers high-resolution and wide-range capabilities,with promising advancements in various sensing applications.

关键词： resonator resolution high

Optical second-harmonic generation imaging for identifying gastrointestinal stromal tumors

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Journal of Innovative Optical Health Sciences 2023年第5期16卷 139-146页

作者： Shichao Zhang Xingxin Huang Deyong kang Jikui Miao Zhenlin Zhan Guoxian Guan jianxin Chen Yongjian Zhou Lianhuang Li Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education Fujian Provincial Key Laboratory for Photonics Technology Fujian Normal University Fuzhou 350007P.R.China Department of Pathology Fujian Medical University Union Hospital Fuzhou 350001 P.R.China Department of Colorectal Surgery The First Affiliated Hospital of Fujian Medical University Fuzhou 350001 P.R.China Department of Gastric Surgery Fujian Medical University Union Hospital Fuzhou 350001 P.R.China

Gastrointestinal stromal tumors(GISTs)are the most common mesenchymal tumors arising in the digest *** brings a challenge to diagnosis because it is asymptomatic *** is well known that tumor development is often accompanied by the changes in the morphology of collagen ***,an emerging optical imaging technique,second-harmonic generation(SHG),can directly identify collagen fibers without staining due to its noncentrosymmetric ***,in this study,we attempt to assess the feasibility of SHG imaging for detecting GISTs by monitoring the morphological changes of collagen fibers in tumor *** found that collagen alterations occurred obviously in the GISTs by comparing with normal tissues,and furthermore,two morphological features from SHG images were extracted to quantitatively assess the morphological difference of collagen fibers between normal muscular layer and GISTs by means of automated image *** analyses show a significant difference in the two collagen *** study demonstrates the potential of SHG imaging as an adjunctive diagnostic tool for label-free identification of GISTs.

关键词： Multiphoton imaging two-photon excited fluorescence second-harmonic generation gastrointestinal stromal tumors.