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肿瘤特异性多肽探针及其在生物成像中的应用

化学学报 2024年第3期82卷 367-376页

作者：王博蔡向东肖建喜兰州大学化学化工学院兰州730000 甘肃省医用胶原蛋白工程研究中心兰州730000

肿瘤是由细胞、细胞外基质及微环境等多因素组成的复杂系统,不同因素在肿瘤的发生和发展中发挥关键作用.肿瘤细胞、细胞外基质及微环境的特异性分析对于肿瘤的精准诊断和靶向治疗至关重要.多肽探针具有特异性高、生物相容性好、组织穿... 详细信息

肿瘤是由细胞、细胞外基质及微环境等多因素组成的复杂系统,不同因素在肿瘤的发生和发展中发挥关键作用.肿瘤细胞、细胞外基质及微环境的特异性分析对于肿瘤的精准诊断和靶向治疗至关重要.多肽探针具有特异性高、生物相容性好、组织穿透能力强和易于制备等优点,已被广泛用于肿瘤的特异性成像研究.本文综述了多肽探针对肿瘤细胞、免疫细胞等细胞靶点的特异性成像;并介绍了以胶原蛋白、纤维蛋白等外基质蛋白为靶点的肿瘤特异性多肽探针的成像研究.本文还总结了对肿瘤微环境中弱酸性、高酶活性等因素响应的肿瘤特异性多肽探针及其生物成像应用.最后,本文总结并讨论了肿瘤特异性多肽探针所面临的挑战与机遇,展望了其在肿瘤精准诊断和个性化治疗领域的前景.

关键词：肿瘤肿瘤微环境细胞外基质多肽探针生物成像

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异源三聚体胶原多肽:设计、表征和应用

中国科学：化学 2024年第3期54卷 370-384页

作者：权思齐张蓝月张子勇孙秀霞肖建喜兰州大学化学化工学院兰州730000 兰州大学药学院兰州730000

胶原蛋白是人体内含量最丰富的蛋白质,其中异源三聚体胶原蛋白含量超过90%.异源三聚体胶原蛋白任意一条肽链的甘氨酸发生突变都会引发遗传疾病.胶原蛋白分子量大且结构复杂,在分子层面研究胶原蛋白存在挑战.胶原多肽的分子量小且结构易... 详细信息

胶原蛋白是人体内含量最丰富的蛋白质,其中异源三聚体胶原蛋白含量超过90%.异源三聚体胶原蛋白任意一条肽链的甘氨酸发生突变都会引发遗传疾病.胶原蛋白分子量大且结构复杂,在分子层面研究胶原蛋白存在挑战.胶原多肽的分子量小且结构易于控制,被广泛应用于仿生胶原蛋白.此外异源三聚体胶原多肽可以精确模拟Ⅰ型和Ⅳ型等异源三聚体胶原蛋白的组成、结构和功能.本综述介绍了异源三聚体胶原多肽的设计方法、表征手段以及应用.首先总结了共价键连接和非共价相互作用这两种构建异源三聚体胶原多肽的策略;其次概述了异源三聚体胶原多肽的肽链组成、三螺旋结构以及热稳定性的表征方法;接着介绍了异源三聚体胶原多肽在模拟胶原蛋白肽链排列、仿生胶原蛋白纤维结构以及研究成骨不全症机制等方面的应用;最后展望了功能化的异源三聚体胶原多肽的开发及其在生物医学领域内的应用前景.

关键词：胶原蛋白多肽异源三聚体设计表征应用

Recent advances in DNA glycosylase assays

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Chinese Chemical Letters 2022年第8期33卷 3603-3612页

作者： Lili Wang Huige Zhang Wei Chen Hongli Chen jianxi xiao Xingguo Chen State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou 730000China

Genomic deoxyribonucleic acid(DNA)is selected as the ideal carrier for preserving and transmitting the genetic information over the course of ***,the genomic DNA is constantly exposed to various endogenous and environmental threats,causing a diversity of damaged bases,lesions,mismatches and base-pair modifications in the genome,eventually leading to genomic instability and *** excision repair(BER)is the most important repair mechanism,repairing a variety of DNA damages arising from oxidation,alkylation,methylation,deamination,and hydrolysis *** glycosylases are responsible for initiating the first step of the BER pathway through cleaving the N-glycosidic bond between the damaged base and the DNA ***,abnormal DNA glycosylases are associated with a variety of diseases such as cancer,cardiovascular disease,neurological disease and inflammation,suggesting the important role of DNA glycosylases in cancer diagnosis and ***,it is highly desirable to monitor the activity of DNA glycosylases,gaining a deep understanding of the restoration process of damaged DNA and clinical ***,a series of novel DNA glycosylases detection methods with excellent performance have been *** this minireview,we summarize the recent advances in DNA glycosylase assays including amplification-free assay and amplification-assisted ***,a brief introduction of amplification-free assay for DNA glycosylase is ***,amplification-assisted assays for DNA glycosylases are discussed in ***,the conclusion and prospects of the directions of DNA glycosylase assays are provided.

关键词： DNA glycosylases Base excision repair Polymerase chain reaction Isothermal signal amplification Nanomaterial-based biosensors

Recent progresses of collagen dressings for chronic skin wound healing

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Collagen and Leather 2023年第4期5卷 23-43页

作者： Shuangni Shi Lili Wang Chen Song Linyan Yao jianxi xiao State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou 730000GansuChina Gansu Engineering Research Center of Medical Collagen Lanzhou 730000GansuChina Department of Chemistry University of ConnecticutStorrsConnecticut 06269USA

The skin plays a fundamental role in regulating the body's internal balance and protecting against external traumas.A broad variety of environmental risk factors frequently result in acute skin wounds,whose inappropriate treatments would lead to chronic skin wounds that are difficult to *** dressings have been widely used to repair chronic skin wounds,however their drawbacks such as insufficient hemostatic efficacy and non-moist environment have severely limited their clinical *** the principal component of skin,collagen has always been a research hotspot in the field of chronic skin wounds due to its advantages of low antigenicity,high biocompatibility and superior ***-based dressings have been increasingly developed to heal the chronic wounds during the past decades,arising from their capability in decreasing protein and electrolyte losses in wound exudate,preventing bacterial contamination,permitting less painful dressing changes,and improving the healing *** review overviews recent progress of collagen dressings for chronic skin wound *** commonly used wound dressings for wound management have been first *** wound dressings have been categorized as films,sponges,hydrogels,nanofibers,and powders,and their efficacy has been *** critical functions of collagen dressings in wound healing,such as stopping bleeding,shortening inflammation,promoting angiogenesis,and stimulating tissue regeneration have been *** clinical applications of collagen dressings to repair different types of chronic wounds have been thoroughly summarized.A comprehensive list of commercialized collagen dressings has been updated,and an outlook of collagen dressings have been finally speculated.

关键词： Collagen Wound dressings Chronic skin wounds Wound healing

Synthesis and Characterization of Fluorescent Silica Nanoparticles Functionalized with Collagens of Variable Lengths

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Chinese Journal of Chemistry 2016年第8期34卷 788-794页

作者： Shanshan Zhang Shenglong Ding Yong Qian Miaomiao Yuan Jun Lu Zaiman Liu jianxi xiao School of Chemical and Biological Engineering Lanzhou Jiaotong University Lanzhou Gansu 730070 China State Key Laboratory of Applied Organic Chemistry Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province College of Chemistry and Chemical Engineering Lanzhou University Lanzhou Gansu 730000 China Institute of Pathogenic Biology School of Basic Medical Sciences Lanzhou University Lanzhou Gansu 730000 China

Collagen, the most abundant protein in human body, has been widely used as an excellent natural material for diverse biomedical applications due to its superior properties such as ample biological interaction sites, minimal immunogenicity and high biocompatibility. Collagens of different lengths are produced by recombinant technology and utilized to functionalize fluorescent silica nanoparticles (FNPs). The collagen‐functionalized FNPs display mono‐disperse distribution, but their sizes are dependent on the length of collagen. These modified FNPs all show nice fluorescence profile as well as low cytotoxicity, suggesting promising applications in bioimaging. We have demonstrated that various types of collagen, conveniently produced by recombinant technology, can be used to modify silica nanoparticles with nice characteristics such as mono‐dispersion, non‐interference in fluorescence and low toxicity. It may endow fluorescent silica nanoparticles with broad biological applications.

关键词： collagen fluorescent silica nanoparticles recombinant technology functionalization

Mg2+-Triggered and pH-Tuned in vitro Assembly of Trehalose-6-Phosphate Synthase

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Wuhan University Journal of Natural Sciences 2018年第5期23卷 396-402页

作者： ZHANG Shanshan YANG Fan ZHANG Yuping LIU Zaiman YU Linghui CHEN Ximing xiao jianxi College of Chemistry and Chemical Engineering Lanzhou University Lanzhou 730000 Gansu China School of Chemical and Biological Engineering Lanzhou Jiaotong University Lanzhou 730070 Gansu China School of Stomatology Lanzhou University Lanzhou 730000 Gansu China Key Laboratory of Desert & Desertification Cold & Arid Regions Environmental & Engineering Research Institute Chinese Academy of Sciences Lanzhou 730000 Gansu China

The enzyme Ots A（trehalose-P synthase） plays a critical role in the biosynthesis of trehalose, which is a nonreducing disaccharide that plays important functions in many organisms. By using light scattering technique, we discovered that Ots A in Arthrobacter strain A3 polymerized in the presence of divalent metal ions（Mg2＋ or Ca2＋）, and the kinetics of the assembly was dependent on their concentrations. We identified potential compounds that can affect the kinetics of the polymerization, particularly, heparin, which acts as a very promising inhibitor of the polymerization. The Ots A assembly turns out to be a very delicate process that is finely regulated by p H. Ots A may be in the polymerized form at physiological pH in vivo, suggesting a more complicated mechanism of the enzyme. These unique properties of Ots A provide novel insights into the molecular mechanism of the biosynthesis of trehalose.

关键词： OtsA(trehalose-P synthase) trehalose polymerization light scattering

The least stable pathological collagen targeted antibody-mimetic-peptide monitors collagen lesions in tumor tissues

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The least stable pathological collagen targeted antibody-mim...

Self-Assembly of Charged Collagen Mimetic Peptide into Biofunctional Nanofiber

第十一届全国化学生物学学术会议

作者： Xiangdong Cai jianxi xiao State Key Laboratory of Applied Organic Chemistry Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu ProvinceCollege of Chemistry and Chemical EngineeringLanzhou University

Collagen is the most important structural protein in the human body. It has shown that collagen lesion is a critical step in tumor invasion and metastasis. [1] It has been demonstrated that pathological collagen targeted antibody-mimetic-peptide（PCTP） is good at binding to denatured collagen, whereas conventional probes form a triple helix that loses capability. [2] Here, we reported that the least stable PCTP remained lasting single-chain and used to monitor the process of collagen lesions in tumors.

关键词： pathological collagen tumor tissue like-antibody peptide

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Self-Assembly of Charged Collagen Mimetic Peptide into Biofu...

Four development stages of collective intelligence

第十一届全国化学生物学学术会议

作者： Linyan Yao Jingyuan Yu Biyang Ling jianxi xiao State Key Laboratory of Applied Organic Chemistry Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu ProvinceCollege of Chemistry and Chemical EngineeringLanzhou University

Collagen,the most abundant protein in the human body,which play an essential role in cell adhesion and growth. Since native collagen is difficult to modify and can engender pathogenic and immunological side effects. Collagen mimetic scaffolds play a pivotal role in regenerative medicine and tissue engineering. We have herein for the first time reported the design of charged collagen mimetic peptides(CMPs),that self-assemble into nanofiber,which well mimic the characteristic D-periodic architectural structure of native *** addition,the CMP conjugated with the integrin specific GFOGER sequence was shown to promote collagen mimetic cell adhesion and *** self-assembled peptide nanofiber was shown to have the ability to structurally and biologically mimic native collagen fiber,suggesting promising applications in tissue regeneration applications.

关键词： charged collagen mimetic peptide self-assembly nanofiber cell adhesion

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Frontiers of Information Technology & Electronic Engineering 2024年第7期25卷 903-916页

作者： Renbin xiao School of Artificial Intelligence and Automation Huazhong University of Science and TechnologyWuhan 430074China Key Laboratory of Image Information Processing and Intelligent Control Ministry of EducationWuhan 430074China

The new generation of artificial intelligence(AI)research initiated by Chinese scholars conforms to the needs of a new information environment changes,and strives to advance traditional artificial intelligence(AI 1.0)to a new stage of AI *** one of the important components of AI,collective intelligence(CI 1.0),i.e.,swarm intelligence,is developing to the stage of CI 2.0(crowd intelligence).Through in-depth analysis and informative argumentation,it is found that an incompatibility exists between CI 1.0 and CI ***,CI 1.5 is introduced to build a bridge between the above two stages,which is based on biocollaborative behavioral *** 1.5 is the transition from CI 1.0 to CI 2.0,which contributes to the compatibility of the two ***,a new interpretation of the meta-synthesis of wisdom proposed by Qian Xuesen is *** meta-synthesis of wisdom,as an improvement of crowd intelligence,is an advanced stage of bionic intelligence,i.e.,CI *** is pointed out that the dual-wheel drive of large language models and big data with deep uncertainty is an evolutionary path from CI 2.0 to CI 3.0,and some elaboration is *** a result,we propose four development stages(CI 1.0,CI 1.5,CI 2.0,and CI 3.0),which form a complete framework for the development of *** different stages are progressively improved and have good *** to the dominant role of cooperation in the development stages of CI,three types of cooperation in CI are discussed:indirect regulatory cooperation in lower organisms,direct communicative cooperation in higher organisms,and shared intention based collaboration in *** division is the main form of achieving cooperation and,for this reason,this paper investigates the relationship between the complexity of behavior and types of labor ***,based on the overall understanding of the four development stages of CI,the future development direction and research issues of CI are explored.

关键词： Collective intelligence Meta-synthesis of wisdom Incompatibility Labor division Cooperative behavior Collective intelligence emergence Large language model