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A chromosome-level genome assembly of the wild rice Oryza rufipogon facilitates tracing the origins of Asian cultivated rice

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Science China(Life Sciences) 2021年第2期64卷 282-293页

作者： Xianrong Xie Huilong Du Huiwu tang jianian tang Xiyu Tan Weizhi Liu Tie Li Zhansheng Lin Chengzhi Liang Yao-Guang Liu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Life SciencesSouth China Agricultural UniversityGuangzhou 510642China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642China University of Chinese Academy of Sciences Beijing 100049China College of Agriculture and Biology Zhongkai University of Agriculture and EngineeringGuangzhou 510225China

Oryza rufipogon *** a wild progenitor of the Asian cultivated rice Oryza *** better understand the genomic diversity of the wild rice,high-quality reference genomes of *** populations are needed,which also facilitate utilization of the wild genetic resources in rice *** this study,we generated a chromosome-level genome assembly of *** using a combination of short-read sequencing,single-molecule sequencing,BioNano and Hi-C *** genome sequence(399.8 Mb)was assembled into 46 scaffolds on the 12 chromosomes,with contig N50 and scaffold N50 of 13.2 Mb and 20.3 Mb,*** genome contains 36,520 protein-coding genes,and 49.37% of the genome consists of repetitive *** genome has strong synteny with those of the *** subspecies indica and japonica,but containing some large structural *** analysis unveiled the polyphyletic origins of ***,in which the japonica and indica genome formations involved different divergent ***(including ***)lineages,accompanied by introgression of genomic regions between japonica and *** high-quality reference genome provides insight on the genome evolution of the wild rice and the origins of the *** subspecies,and valuable information for basic research and rice breeding.

关键词： genome genome sequencing evolution Oryza rufipogon rice

OsLTP47 may function in a lipid transfer relay essential for pollen wall development in rice

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Journal of Genetics and Genomics 2022年第5期49卷 481-491页

作者： Libin Chen Chonghui Ji Degui Zhou Xin Gou jianian tang Yongjie Jiang Jingluan Han Yao-Guang Liu Letian Chen Yongyao Xie State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Life SciencesSouth China Agricultural UniversityGuangzhouGuangdong 510642China Guangdong Laboratory for Lingnan Modern Agriculture GuangzhouGuangdong 510642China Guangdong Key Laboratory of New Technology in Rice Breeding Rice Research InstituteGuangdong Academy of Agricultural SciencesGuangzhouGuangdong 510640China Present address:Vector-borne Virus Research Center Fujian Province Key Laboratory of Plant VirologyFujian Agriculture and Forestry UniversityFuzhouFujian 350002China.

In plants,lipid transfer proteins(LTPs)transport pollen wall constituents from the tapetum to the exine,a process essential for pollen wall ***,the functional cooperation of different LTPs in pollen wall development is not well *** this study,we have identified and characterized a grassspecific LTP gene,Os LTP47,an important regulator of pollen wall formation in rice(Oryza sativa).Os LTP47 encodes a membrane-localized LTP and in vitro lipid-binding assays confirms that Os LTP47 has lipidbinding *** of Os LTP47 causes disordered lipid metabolism and defective pollen walls,leading to male *** two-hybrid and pull-down assays reveal that Os LTP47 physically interacts with another LTP,Os *** findings suggest that the plasma membrane-localized Os LTP47 may function as a mediator in a lipid transfer relay through association with cytosolic and/or locular Os C6 for pollen wall development and that various LTPs may function in a coordinated manner to transport lipid molecules during pollen wall development.

关键词： Rice Pollen wall Lipid transfer protein Male sterility

Potential role of tanycyte-derived neurogenesis in Alzheimer's disease

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Neural Regeneration Research 2025年第6期20卷 1599-1612页

作者： Guibo Qi Han tang jianian Hu Siying Kang Song Qin Department of Anatomy Histologyand EmbryologySchool of Basic Medical SciencesFudan UniversityShanghaiChina State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Fudan UniversityShanghaiChina

Tanycytes, specialized ependymal cells located in the hypothalamus, play a crucial role in the generation of new neurons that contribute to the neural circuits responsible for regulating the systemic energy balance. The precise coordination of the gene networks controlling neurogenesis in naive and mature tanycytes is essential for maintaining homeostasis in adulthood. However, our understanding of the molecular mechanisms and signaling pathways that govern the proliferation and differentiation of tanycytes into neurons remains limited. This article aims to review the recent advancements in research into the mechanisms and functions of tanycyte-derived neurogenesis. Studies employing lineage-tracing techniques have revealed that the neurogenesis specifically originating from tanycytes in the hypothalamus has a compensatory role in neuronal loss and helps maintain energy homeostasis during metabolic diseases. Intriguingly,metabolic disorders are considered early biomarkers of Alzheimer's disease. Furthermore,the neurogenic potential of tanycytes and the state of newborn neurons derived from tanycytes heavily depend on the maintenance of mild microenvironments, which may be disrupted in Alzheimer's disease due to the impaired blood–brain barrier ***, the specific alterations and regulatory mechanisms governing tanycyte-derived neurogenesis in Alzheimer's disease remain unclear. Accumulating evidence suggests that tanycyte-derived neurogenesis might be impaired in Alzheimer's disease, exacerbating neurodegeneration. Confirming this hypothesis, however, poses a challenge because of the lack of long-term tracing and nucleus-specific analyses of newborn neurons in the hypothalamus of patients with Alzheimer's disease. Further research into the molecular mechanisms underlying tanycyte-derived neurogenesis holds promise for identifying small molecules capable of restoring tanycyte proliferation in neurodegenerative diseases. This line of investigation could pro

关键词： Alzheimer's disease blood–brain barrier ependymoglial cells hypothalamus metabolic diseases neural stem cells neurogenesis neuroinflammatory diseases neurons tanycyte

Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants

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Science China(Life Sciences) 2020年第6期63卷 933-935页

作者： Yu Hao Wubei Zong Dongchang Zeng Jingluan Han Shuifu Chen jianian tang Zhe Zhao Xiaojuan Li Kun Ma Xianrong Xie Qinlong Zhu Yuanling Chen Xiucai Zhao Jingxin Guo Yao-Guang Liu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangzhou 510642.China College of Life Sciences South China Agricultural UniversityGuangzhou 510642.China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642.China

Dear Editor,CRISPR(clustered regularly interspaced short palindromic repeats)/Cas genome editing is a powerful tool for introducing specific mutations in organisms including *** system is composed of a nuclease such as Cas9 or Cas12a and an engineered single-guide RNA(sgRNA)incorporating a target sequence(Li et al.,2019).A Cas9/sgRNA complex recognizes its target site in the genome,resulting in a mutation at that site.

关键词： RNA CRISPR Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants

A　Preliminary　Study　of　Dielectric　Properties　and　Structures　of　NBT-SCN　System

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Wuhan University Journal of Natural Sciences 1997年第1期2卷 129-130页

作者： tang, Yiwen Dai, Mingxing Wang, Renhui Gui, jianian Wuhan University Department of Physics Wuhan China (GRID:grid.49470.3e) (ISNI:***)

ＡＰｒｅｌｉｍｉｎａｒｙＳｔｕｄｙｏｆＤｉｅｌｅｃｔｒｉｃＰｒｏｐｅｒｔｉｅｓａｎｄＳｔｒｕｃｔｕｒｅｓｏｆＮＢＴ┐ＳＣＮＳｙｓｔｅｍＴａｎｇＹｉｗｅｎ，ＤａｉＭｉｎｇｘｉｎｇ，ＷａｎｇＲｅｎｈｕｉ，ＧｕｉＪｉａｎｉ... 详细信息

ＡＰｒｅｌｉｍｉｎａｒｙＳｔｕｄｙｏｆＤｉｅｌｅｃｔｒｉｃＰｒｏｐｅｒｔｉｅｓａｎｄＳｔｒｕｃｔｕｒｅｓｏｆＮＢＴ┐ＳＣＮＳｙｓｔｅｍＴａｎｇＹｉｗｅｎ，ＤａｉＭｉｎｇｘｉｎｇ，ＷａｎｇＲｅｎｈｕｉ，ＧｕｉＪｉａｎｉａｎＤｅｐａｒｔｍｅｎｔｏｆＰｈ...

关键词： Dielectric Study System Preliminary SCN NBT-SCN

四川盆地二叠系永探1井火山岩气井完井测试井壁稳定性实验

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天然气工业 2022年第3期42卷 91-98页

作者：唐庚龚浩徐家年周朗张华礼张林中国石油西南油气田公司工程技术研究院重庆科技学院石油与天然气工程学院中国石油西南油气田公司工程技术处

四川盆地二叠系永探1井火山岩气井,在完井试油过程中地层垮塌,返出大量火山岩碎屑,堵塞井筒及地面流程,严重影响了完井测试。为进一步优选后续完井方式,高效开发此类气藏,在分析储层岩石矿物组分、微观孔隙结构、岩石力学参数的基础上,... 详细信息

四川盆地二叠系永探1井火山岩气井,在完井试油过程中地层垮塌,返出大量火山岩碎屑,堵塞井筒及地面流程,严重影响了完井测试。为进一步优选后续完井方式,高效开发此类气藏,在分析储层岩石矿物组分、微观孔隙结构、岩石力学参数的基础上,基于Von-Mises剪切破坏理论,进行了完井试油及生产过程中井壁稳定性的研究。研究结果表明:(1)工区火山岩下部储层黏土矿物含量高,裂缝、微裂缝发育,经工作液作用后,易发生黏土矿物膨胀、颗粒分散、运移,引起井壁失稳;(2)不同工作液影响了岩石力学参数,进而影响到井壁稳定性,经改性聚磺试油工作液作用后的储层,直井井壁上岩石最大剪切应力均方根存在大于井壁岩石剪切强度均方根的井壁不稳定点;(3)火山岩下部储层火山岩碎屑产出的临界日产量为1.13×10^(4)m^(3);(4)模拟气井日产量介于1.42×10^(4)~28.35×10^(4)m^(3)时,折算年碎屑产出量介于270.3~2183.2 kg,折算井筒沉砂高度介于12.7~102.5 m,易对生产造成影响。结论认为,实验室结果为此类火山岩气藏的高效开发奠定了基础,火山岩气井完井试油及生产过程中,应通过优化工作液配方、控制生产压差、优选完井方法等方式保障井壁稳定,充分发挥气井产能。

关键词：四川盆地火山岩气井井壁稳定火山碎屑完井试油增产临界压差临界产量

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碎米综合利用的研究

湖北农业科学 2000年第2期39卷 60-61页

作者：唐家礼张家年华中农业大学食品科技系武汉430070

研究以米为原料 ,通过酶水解、分离。

关键词：高蛋白米粉碎米麦芽糊精综合利用加工工艺

10.5%三氟甲吡醚乳油对榕管蓟马室内毒力及田间防效试验

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安徽农学通报 2018年第13期24卷 60-61页

作者：唐家年郑泽群李志坚刘颖吴正伟广东海洋大学农学院广东湛江524088 北京新禾丰农化资料有限公司北京100000

为了探究10.5%三氟甲吡醚乳油(EC)对园林害虫榕管蓟马的毒杀活性并为其田间应用技术提供依据,采用浸叶法进行了室内毒力测定和喷雾法进行田间防效试验。室内毒力测定表明,10.5%三氟甲吡醚EC对榕管蓟马成虫48h LC50值为76.62mg/L。田间... 详细信息

为了探究10.5%三氟甲吡醚乳油(EC)对园林害虫榕管蓟马的毒杀活性并为其田间应用技术提供依据,采用浸叶法进行了室内毒力测定和喷雾法进行田间防效试验。室内毒力测定表明,10.5%三氟甲吡醚EC对榕管蓟马成虫48h LC50值为76.62mg/L。田间防效试验表明,10.5%三氟甲吡醚EC在140mg/L剂量,药后1、3d时防效显著低于添加助剂赛特胜及400mg/L 40%毒死蜱乳油,而在药后7d其防效提高到79.99%。10.5%三氟甲吡醚EC对榕管蓟马具有较好毒杀活性,通过相应助剂的添加使用适合田间推广应用。

关键词：垂叶榕蓟马啶虫丙醚药效

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海南冬季诱导火龙果开花的补光条件

热带生物学报 2019年第1期10卷 60-65页

作者：熊睿徐敏刘成立林家年程玉韦双双汤华海南大学热带农林学院/海南热带生物资源可持续利用国家重点实验室培育基地海口570228

以红肉火龙果(Hylocereus undulatus Britt)‘金都1号’为材料,通过不同的光谱及光照时间处理,研究不同的补光条件对诱导海南冬季火龙果开花的影响。结果表明:1)组合黄光和红光能够诱导火龙果成花,蓝光不能诱导火龙果成花。2)补光时间... 详细信息

以红肉火龙果(Hylocereus undulatus Britt)‘金都1号’为材料,通过不同的光谱及光照时间处理,研究不同的补光条件对诱导海南冬季火龙果开花的影响。结果表明:1)组合黄光和红光能够诱导火龙果成花,蓝光不能诱导火龙果成花。2)补光时间的长短和光谱的交互作用对火龙果成花有显著影响。3)不同月份,诱导火龙果成花所需的补光时间不同。采用15W组合黄光,在2月初至2月下旬,需补光5 h诱导成花;在2月下旬至3月上旬,补光4 h可诱导成花;在3月上旬到3月底,补光3 h就可诱导火龙果成花。

关键词：火龙果冬季诱导成花补光时间补光光谱