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小麦新种质“普冰3228”穗下节长度QTL定位与候选基因分析

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西南农业学报 2024年第2期37卷 250-257页

作者：王健胜王二伟马爱锄程世平平顶山学院河南平顶山467000 河南省生态经济型木本植物种质创新与利用重点实验室河南平顶山467000 平顶山市农业科学院河南平顶山467001

【目的】挖掘小麦新种质“普冰3228”穗下节的有效位点/基因,探讨其穗下节分子遗传机制。【方法】以“普冰3228”×“京4839”杂交产生的210个小麦重组自交系群体为材料,利用55K SNP芯片构建该群体高密度分子遗传连锁图谱,采用完备区间... 详细信息

【目的】挖掘小麦新种质“普冰3228”穗下节的有效位点/基因,探讨其穗下节分子遗传机制。【方法】以“普冰3228”×“京4839”杂交产生的210个小麦重组自交系群体为材料,利用55K SNP芯片构建该群体高密度分子遗传连锁图谱,采用完备区间作图法(ICIM⁃ADD)对穗下节长度进行QTL定位分析,并基于QTL定位结果对穗下节长度候选基因进行预测分析。【结果】研究发现210个小麦重组自交系遗传群体穗下节长度存在丰富的遗传差异,分布于16.00~117.50 cm。共检测到6个与小麦穗下节相关的QTL,该QTL主要分布于2B、4B、4D、5B和6D染色体上,其LOD值介于2.55~7.88,解释变异率分布于3.43%~15.84%,且绝大多数QTL均来自母本“普冰3228”。定位于4B染色体上AX⁃109373490~AX⁃111540511区间的QUIL⁃4B.e1和定位于4D染色体上AX⁃110984743~AX⁃109230716区间的QUIL⁃4D.e1为穗下节长度主效QTL,可在2种环境下均被检测到的QUIL⁃4D为穗下节长度稳定QTL。进一步分析发现15个与穗下节长度相关的候选基因。该候选基因主要编码一些相关蛋白及功能酶,通过调节或影响植物代谢活动进而对小麦穗下节长度产生影响。例如TraesCS4D01G039200可能编码一种原纤维家族蛋白,直接影响小麦穗下节长度,TraesCS4D01G040300可能编码代谢产物转运蛋白,通过调节代谢产物在穗下节区域的分配影响穗下节的长度。【结论】研究共检测到与小麦穗下节长度相关QTL 6个,预测到与穗下节相关候选基因15个,研究结果有助于小麦穗下节遗传机制解析,并为穗下节遗传改良提供新种质。

关键词： “普冰3228” 穗下节长度 QTL定位候选基因分析

Effect of radio frequency bias on plasma characteristics of inductively coupled argon discharge based on fluid simulations

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Chinese Physics B 2020年第9期29卷 369-376页

作者： Xiao-Yan Sun Yu-ru Zhang Sen Chai You-Nian Wang Yan-Yan chu jian-Xin He Textile and Garment Industry of Research Institute Zhongyuan University of TechnologyZhengzhou 450007China Key Laboratory of Materials Modification by Laser Ionand Electron Beams(Ministry of Education)School of PhysicsDalian University of TechnologyDalian 116024China China Special Equipment Inspection and Research Institute Beijing 100029China

t A fluid model is employed to investigate the effect of radio frequency bias on the behavior of an argon inductively coupled plasma(ICP).In particular,the effects of ICP source power,single-frequency bias power,and dual-frequency bias power on the characteristics of ICP are simulated at a fixed pressure of 30 mTorr(1 Torr=1.33322×102 Pa).When the bias frequency is fixed at 27.12 MHz,the two-dimensional(2D)plasma density profile is significantly affected by the bias power at low ICP source power(e.g.,50 W),whereas it is weakly affected by the bias power at higher ICP source power(e.g.,100 W).When dual-frequency(27.12 MHz/2.26 MHz)bias is applied and the sum of bias powers is fixed at 500 W,a pronounced increase in the maximum argon ion density is observed with the increase of the bias power ratio in the absence of ICP source *** the ratio of 27.12-MHz/2.26-MHz bias power decreases from 500 W/0 W to 0 W/500 W with the ICP source power fixed at 50 W,the plasma density profiles smoothly shifts from edge-high to center-high,and the effect of bias power on the plasma distribution becomes weaker with the bias power ratio ***,the axial ion flux at the substrate surface is characterized by a maximum at the edge of the *** the ICP source power is higher,the 2D plasma density profiles,as well as the spatiotemporal and radial distributions of ion flux at the substrate surface are characterized by a peak in the reactor center,and the distributions of plasma parameters are negligibly affected by the dual-frequency bias power ratio.

关键词： fluid simulation single-and dual-frequency bias power plasma distribution

Sparse representation based on projection method in online least squares support vector machines

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控制理论与应用（英文版） 2009年第2期7卷 163-168页

作者： Lijuan LI Hongye SU jian chu State Key Laboratory of Industrial Control Technology Institute of Advanced Process Control Zhejiang University Hangzhou Zhejiang 310027 China College of Automation Nanjing University of Technology Nanjing Jiangsu 210009 China

A sparse approximation algorithm based on projection is presented in this paper in order to overcome the limitation of the non-sparsity of least squares support vector machines （LS-SVM）. The new inputs are projected into the subspace spanned by previous basis vectors （BV） and those inputs whose squared distance from the subspace is higher than a threshold are added in the BV set, while others are rejected. This consequently results in the sparse approximation. In addition, a recursive approach to deleting an exiting vector in the BV set is proposed. Then the online LS-SVM, sparse approximation and BV removal are combined to produce the sparse online LS-SVM algorithm that can control the size of memory irrespective of the processed data size. The suggested algorithm is applied in the online modeling of a pH neutralizing process and the isomerization plant of a refinery, respectively. The detailed comparison of computing time and precision is also given between the suggested algorithm and the nonsparse one. The results show that the proposed algorithm greatly improves the sparsity just with little cost of precision.

关键词： Least squares support vector machines Projection Sparsity pH neutralizing process Isomerization

Delay-dependent stabilization of singular Markovian jump systems with state delay

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控制理论与应用（英文版） 2009年第3期7卷 231-236页

作者： Zhengguang WU Hongye SU jian chu National Laboratory of Industrial Control Technology Institute of Cyber-Systems and Control Zhejiang University Hangzhou Zhejiang 310027 China

This paper deals with the delay-dependent stabilization problem for singular systems with Markovian jump parameters and time delays. A delay-dependent condition is established for the considered system to be regular, impulse free and stochastically stable. Based on the condition, a design algorithm of the desired state feedback controller which guarantees the resultant closed-loop system to be regular, impulse free and stochastically stable is proposed in terms of a set of strict linear matrix inequalities （LMIs）. Numerical examples show the effectiveness of the proposed methods.

关键词： Singular time-delay systems Markovian jumping parameters Delay-dependent Stochastic stability Linear matrix inequality (LMI)

Denaturation behavior of inclusions in industrial pure iron by calcium treatment

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Journal of Iron and Steel Research International 2023年第2期30卷 249-258页

作者： Xin Liu Yan-ping Bao Cheng-jian Hua jian-hua chu Chao Gu Min Wang State Key Lab of Advanced Metallurgy University of Science and Technology BeijingBeijing100083China

Only a few Chinese enterprises can produce high-quality industrial pure iron,and studies on the smelting process of industrial pure iron were *** inclusions in a melting process were characterized by means of electron microscopy and an automatic inclusion analysis system,and the evolution mechanism of inclusion was studied using thermodynamic calculation to optimize the calcium alloy addition in liquid *** results show that during the smelting process,inclusions mainly composed of Al_(2)O_(3)and spinel are formed before calcium *** calcium treatment,they continuously react with[Ca],[S]and[Ti],grow up,and are removed during refining and tundish *** the end,there are more small-sized inclusions containing CaS,and the contents of Al_(2)O_(3)and spinel are *** to thermodynamic calculations,the appropriate calcium treatment liquid window for the molten steel composition is(10-38)×10^(-6).Calcium treatment has changed the main types of inclusions in industrial pure iron from Al_(2)O_(3)to small-sized inclusions containing CaS and effectively reduces the influence of Al_(2)O_(3)inclusions on the quality of industrial pure iron.

关键词： Industrial pure iron Calcium treatment Inclusion Thermodynamic CaS

Robust stability analysis of networked control systems

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控制理论与应用（英文版） 2009年第3期7卷 301-306页

作者： Jinfeng GAO Hongye SU jian chu Institute of Automation Zhejiang Sci-Tech University Hangzhou Zhejiang 310018 China Institute of Advanced Process Control Zhejiang University Hangzhou Zhejiang 310027 China

This paper is concerned with the problem of robust stability analysis for networked control systems （NCSs）. A new NCS model is proposed under consideration of both the network-induced delay and parameter uncertainties. The parameter uncertainties appearing in NCSs are norm-bounded, and possibly time-varying. The conventional method and the descriptor system method are used to obtain maximum allowable delay bound （MADB） guaranteeing robust stability and stability of the NCSs, respectively, where the stability criteria are formulated in terms of linear matrix inequalities （LMIs）. And the MADB can be derived by solving the feasibility problem of the corresponding LMI. Some numerical examples are provided to illustrate the effectiveness of the proposed method.

关键词： Robust control Time-delay system Networked control systems (NCSs) Linear matrix inequality (LMI)

Robust H-infinity control for uncertain discrete-time Markovian jump systems with actuator saturation

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控制理论与应用（英文版） 2011年第4期9卷 465-471页

作者： Ting SHI Hongye SU jian chu Institute of Automation Hangzhou Dianzi University Hangzhou Zhejiang 310018 China State Key Laboratory of Industrial Control Technology Institute of Cyber-Systems and Control Zhejiang University Hangzhou Zhejiang 310027 China

The design of robust H-infinity controller for uncertain discrete-time Markovianjump systems with actuator saturation is addressed in this paper. The parameter uncertainties are assumed to be norm-bounded. Linear matrix inequality （LMI） conditions are proposed to design a set of controllers in order to satisfy the closed-loop local stability and closed-loop H-infinity performance. Using an LMI approach, a set of state feedback gains is constructed such that the set of admissible initial conditions is enlarged and formulated through solving an optimization problem. A numerical example is given to illustrate the effectiveness of the proposed methods.

关键词： Markovian jump systems H-infinity control Actuator saturation LMIs

Stability analysis for continuous-time systems with actuator saturation

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控制理论与应用（英文版） 2009年第4期7卷 352-358页

作者： Ting SHI Hongye SU jian chu State Key Laboratory of Industrial Control Technology Institute of Cyber-Systems and Control Zhejiang University Hangzhou Zhejiang 310027 China

The aim of this paper is to study the determination of the stability regions for continuous-time systems subject to actuator saturation. Using an affine saturation-dependent Lyapunov function, a new method is proposed to obtain the estimation of the domain of attraction of the closed-loop system. A family of linear matrix inequalities （LMIs） that provides sufficient conditions for the existence of this type of Lyapunov function are presented. The results obtained in this paper can reduce the conservativeness compared with the existing ones. Numerical examples are given to illustrate the effectiveness of the proposed results.

关键词： Actuator saturation Stability analysis Domain of attraction Invariant set Linear matrix inequality (LMI)

鳜Shh基因分子特征和时空表达规律及其对肌肉生长的调控

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水生生物学报 2024年第1期48卷 157-165页

作者：孟杨杨朱鑫成嘉陈琳褚武英宾石玉张建社广西师范大学生命科学学院珍稀濒危动植物生态与环境保护教育部重点实验室桂林541006 长沙学院生物与化学工程学院水生动物营养与品质调控湖南省重点实验室长沙410022

研究从鳜(Siniperca chuatsi)基因组中获得Shh(Sonic hedgehog)基因序列,对该基因编码的蛋白质和同源进化特征进行了分析。鳜Shh基因的开放阅读框(ORF)为1242 bp,编码413个氨基酸,分子量为46.01 kD,等电点(pI)为6.57,脂溶系数为82.83,... 详细信息

研究从鳜(Siniperca chuatsi)基因组中获得Shh(Sonic hedgehog)基因序列,对该基因编码的蛋白质和同源进化特征进行了分析。鳜Shh基因的开放阅读框(ORF)为1242 bp,编码413个氨基酸,分子量为46.01 kD,等电点(pI)为6.57,脂溶系数为82.83,亲水性平均系数为−0.292,拥有一个跨膜区,被推定为亲水性膜结合蛋白。Shh蛋白具有两个结构域,即Hh-N和Hh-C结构域。鳜Shh蛋白与自身所属的鲈形目鱼类Shh蛋白同源性较高。通过实时荧光定量PCR技术检测了鳜Shh基因的时空表达水平。结果显示:Shh的表达量在神经胚期表达显著上调,并在胚胎发育中后阶段保持较高水平。Shh在鳜不同组织中存在一定的表达差异,在脑、肠道中表达量较高,而在红肌、白肌等组织中表达量相对较低。通过环巴胺(Cyclopamine)处理鳜胚胎来抑制Shh信号通路的实验表明,Shh信号通路被抑制后,Pax3、Pax7、Myomaker、生肌调节因子及肌球蛋白重、轻链等基因的表达均显著降低,推测Shh参与调控鳜肌细胞的早期分化和发育过程。研究将有助于从分子水平了解Shh信号调控鱼类发育的分子机理,为鱼类发育生物学以及健康养殖提供参考依据。

关键词： Shh 胚胎发育骨骼肌表达特征鳜

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