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Efficacy of digital breast tomosynthesis combined with magnetic resonance imaging in the diagnosis of early breast cancer

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World Journal of Clinical Cases 2022年第28期10卷 10042-10052页

作者： Yun Ren Jiao Zhang Jin-Dan Zhang jian-zhong xu Department of Breast Surgery Changzhi People's Hospital Affiliated to Shanxi Medical UniversityChangzhi 046000Shanxi ProvinceChina Department of Diagnostic Radiology Changzhi People's Hospital Affiliated to Shanxi Medical UniversityChangzhi 046000Shanxi ProvinceChina

BACKGROUND The incidence and mortality rate of breast cancer in China rank 120th and 163rd,worldwide,*** incidence of breast cancer is on the rise;the risk increases with age but is slightly reduced after *** screening,diagnosis,and timely determination of the best treatment plan can ensure clinical efficacy and *** To evaluate the clinical value of magnetic resonance imaging(MRI) combined with digital breast tomosynthesis(DBT) in diagnosing early breast cancer and the effect of breast-conserving surgery by arc *** This study was divided into two ***,110 patients with early breast cancer confirmed by pathological examination and 110 with benign breast diseases diagnosed simultaneously in Changzhi People’s Hospital of Shanxi Province and Shanxi Dayi Hospital from May 2019 to September 2020 were included in the breast cancer group and the benign group,*** groups underwent DBT and MRI examination,and the pathological results were used as the gold standard to evaluate the effectiveness of the combined application of DBT and MRI in the diagnosis of early breast ***,according to the operation method,110 patients with breast cancer were divided into either a breast-conserving group(69 patients) or a modified radical mastectomy group(41 patients).The surgical effect,cosmetic effect,and quality of life of the two groups were *** Among the 110 cases of breast cancer,66 were of invasive ductal carcinoma(60.00%),and 22 were of ductal carcinoma in situ(20.00%).Among the 110 cases of benign breast tumors,55 were of breast fibromas(50.00%),and 27 were of breast adenosis(24.55%).The sensitivity,specificity,and area under the curve(AUC) of DBT in the differential diagnosis of benign and malignant breast tumors were 73.64%,84.55%,and 0.791,*** sensitivity,specificity,and AUC of MRI in the differential diagnosis of benign and malignant breast tumors were 84.55%,85.45%,and 0.85

关键词： Breast cancer Magnetic resonance Digital mammography Clinical value Arc incision Breastconserving surgery Digital breast tomosynthesis

Accelerating the menaquinone‑7 production in Bacillus amyloliquefaciens by optimization of the biosynthetic pathway and medium components

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Systems Microbiology and Biomanufacturing 2023年第4期3卷 776-791页

作者： Chang‑Long Li Meng Li Wei‑Guo Zhang jian‑zhong xu The Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan University1800#Lihu RoadWuXi 214122People’s Republic of China

Menaquinone-7(MK-7)has an important role in preventing diseases such as cardiovascular disease and *** this study,a combination strategy of strain improvement and medium optimization is investigated to increase MK-7 production in Bacillus *** breeding method was first used to modify the biosynthetic pathway to construct a MK-7 high-producing strain by atmospheric and room temperature plasma mutagenesis and protoplast *** resulted strain Ba-4 with resistance to sulfaguanidine,1-hydroxy-2-naphthoic acid,menadione,2-deoxy-d-glucose and rifampicin as well as sensitive toβ-fluoropyruvate produced 73.57±1.61 mg/L of MK-7,which was 1.36 times more than that of the parent strain H.β.D.R.-5(i.e.,31.12±1.40 mg/L).Subsequently,single-factor optimization and response surface method-ology(RSM)were used to optimize the medium components for increasing MK-7 production by strain *** Ba-4 produced 90.43±1.32 mg/L of MK-7 under the single-factor optimized ***,the results of response surface methodology indicated that glycerol,soy peptone and Tween-80 had significant effects on MK-7 production,and the highest MK-7 production(i.e.,95.03±1.01 mg/L)was obtained under the optimized medium,which was 0.29 times higher than that of the initial *** results confirmed that the conventional breeding methods and fermenter control system are effective strategies in improving MK-7 production by ***.

关键词： Bacillus amyloliquefaciens Menaquinone-7 ARTP mutagenesis system Protoplast fusion Medium optimization

Interface Analysis and Hot Deformation Behaviour of a Novel Laminated Composite with High-Cr Cast Iron and Low Carbon Steel Prepared by Hot Compression Bonding

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Journal of Iron and Steel Research International 2015年第5期22卷 438-445页

作者： Zheng-yi jianG Xing-jian GAO Sheng-li LI Hong-mei ZHANG Deng-fu CHEN jian-zhong xu School of Materials and Metallurgy University of Science and Technology Liaoning School of Mechanical Materials and Mechatronic EngineeringUniversity of Wollongong College of Materials Science and Engineering Chongqing University State Key Laboratory of Rolling and Automation Northeastern University

A hot compression bonding process was developed to prepare a novel laminated composite consisting of high-Cr cast iron （HCCI） as the inner layer and low carbon steel （LCS） as the outer layers on a Gleeble 3500 ther- momechanicat simulator at a temperature of 950 ℃ and a strain rate of 0. 001 s 1. Interfacial bond quality and hot deformation behaviour of the laminate were studied by mierostructural characterisation and mechanical tests. Experi- mental results show that the metallurgical bond between the constituent metals was achieved under the proposed bonding conditions without discernible defects and the formation of interlayer or intermetallic layer along the inter- face. The interfacial bond quality is excellent since no deterioration occurred around the interface which was deformed by Vickers indentation and compression test at room temperature with parallel loading to the interface. After well cladding by the LCS, the brittle HCCI can be severely deformed （about 57 % of reduction） at high temperature with crack-free. This significant improvement should be attributed to the decrease of crack sensitivity due to stress relief by soft claddings and enhanced flow property of the HCCI by simultaneous deformation with the LCS.

关键词： bonding laminated metal composite interface structure hot working high-Cr cast iron

Effect of vitamin D3 on maturation and antigen-presenting function of dendritic cells treated with Mycobacterium tuberculosis

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Asian Pacific Journal of Tropical Medicine 2016年第1期9卷 51-54页

作者： Chun-Yu Liu Ze-Hua Zhang Hui-Feng Yang xu Hui Fei-Fan Cheng jian-zhong xu No 202 Hospital of People's Liberation Army Department of Orthopedics.Southwest Hospital Third Military Medical Department of Orthopedics.General Hospital of Shenyang Military

Objective:To investigate the phenotypic characteristics and functional capability differences of mouse bone marrow-derived dendritic cells after stimulation with Mycobacterium tuberculosis in the presence or absence of vitamin ***:Mouse bone marrow-derived cells were cultured with GM-CSF(20 ng/mL).Then,one was added with 100 nmol/L of 25(OH)D3,while the other did *** day 6.5 μg/mL of BCG was added to stimulate the cells for 24 *** day 7,suspension cells were harvested for phenotypic and functional ***:The percentages of CD86 dendritic cells(DCs) in the control group and 25(OH)D3 group were 66.97%± 8.29%and 52.18%± 8.52%.respectively;the mean fluorescence intensities of MHC- Ⅱ in the control group and 25(OH)D3 group were 1 102.16 ± 371.02 and 681.62± *** expression levels of MHC- Ⅱ and CD86 on the surface of the DCs in 25(OH)D3 group were significantly lower than those of the control *** ability of the DCs to stimulate proliferation of T-lymphocytes was also significantly lower than that of the control ***:These findings suggest that 25(OH)D3 modulates the immune response by affecting the maturation and function of DCs in Mycobacterium tuberculosis period.

关键词： 25(OH)D3 Dendritic cells Mycobacterium tuberculosis

Menaquinone-7 production from maize meal hydrolysate by Bacillus isolates with diphenylamine and analogue resistance

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第6期18卷 462-473页

作者： jian-zhong xu Wei-guo ZHANG The Key Laboratory of lndustrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China

A menaquinone-7（MK-7） high-producing strain needs to be isolated to increase MK-7 production, in order to meet a requirement of MK-7 given the low MK-7 content in food products. This article focuses on developing MK-7 high-producing strains via screening and mutagenesis by an atmospheric and room temperature plasma（ARTP） mutation breeding system. We isolated an MK-7-producing strain Y-2 and identified it as Bacillus amyloliquefaciens, which produced（7.1±0.5） mg/L of MK-7 with maize meal hydrolysate as carbon source. Then, an MK-7 highproducing strain B. amyloliquefaciens H.β.D.R.-5 with resistance to 1-hydroxy-2-naphthoic acid, β-2-thienylalanine, and diphenylamine was obtained from the mutation of the strain Y-2 using an ARTP mutation breeding system. Using strain H.β.D.R.-5, efficient production of MK-7 was achieved（（30.2±2.7） mg/L）. In addition, the effects of nitrogen sources, prenyl alcohols, and MgSO_4 on MK-7 production were investigated, suggesting that soymeal extract combined with yeast extract, isopentenol, and MgSO4 was beneficial. Under the optimized condition, the MK-7 production and biomass-specific yield reached（61.3±5.2） mg/L and 2.59 mg/L per OD600 unit respectively in a 7-L fermenter. These results demonstrated that strain H.β.D.R.-5 has the capacity to produce MK-7 from maize meal hydrolysate, which could reduce the substrate cost.

关键词： Menaquinone-7 Bacillus amyloliquefaciens Analog resistance Diphenylamine resistance Maize meal hydrolysate

Strategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expression

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第2期17卷 83-99页

作者： jian-zhong xu Wei-guo ZHANG The Key Laboratory of Industrial Biotechnology Ministry of Education School of Biotechnology Jiangnan University

With the availability of the whole genome sequence of Escherichia coli or Corynebacterium glutamicum, strategies for directed DNA manipulation have developed rapidly. DNA manipulation plays an important role in understanding the function of genes and in constructing novel engineering bacteria according to requirement. DNA manipulation involves modifying the autologous genes and expressing the heterogenous genes. Two alternative approaches, using electroporation linear DNA or recombinant suicide plasmid, allow a wide variety of DNA manipulation. However, the over-expression of the desired gene is generally executed via plasmid-mediation. The current review summarizes the common strategies used for genetically modifying E. coli and C. glutamicum genomes, and discusses the technical problem of multi-layered DNA manipulation. Strategies for gene over-expression via integrating into genome are proposed. This review is intended to be an accessible introduction to DNA manipulation within the bacterial genome for novices and a source of the latest experimental information for experienced investigators.

关键词： Escherichia coli Corynebacterium glutamicum DNA manipulation Site-directed mutagenesis Gene inactivation Gene over-expression

Recent progress in self-propelled particles

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Journal of Hydrodynamics 2024年第1期36卷 61-77页

作者： Zhen-yu Ouyang jian-zhong Lin Laboratory of Impact and Safety Engineering Ministry of EducationNingbo UniversityNingbo315201China Department of Mechanics State Key Laboratory of Fluid Power and Mechatronic SystemsZhejiang UniversityHangzhou310027China

Self-propelled particles are commonly found in a large number of planktonic organisms such as bacteria,fungi,and algae in nature,and researchers have taken a long interest in exploring their swimming mechanisms for more than a *** in the past 20 years,with the development of computational fluid dynamics and flow display technology,as well as the need for the design of synthetic self-propelled particles and micro-swimming devices,self-propelled particles have become the forefront and hotspot of current research in the field of fluid *** paper first introduces the swimming characteristics of common self-propelled particles,leading to a classic“squirmer”type self-propelled particle *** this basis,a systematic introduction and summary of the theoretical and numerical simulation research of“squirmer”will be ***,the main challenges and opportunities faced by the current research will be summarized.

关键词： Self-propelled particles multiphase flow hydrodynamic interaction

Antisense RNA: the new favorite in genetic research

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2018年第10期19卷 739-749页

作者： jian-zhong xu Jun-lan ZHANG Wei-guo ZHANG The Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China Department of ln Vitro Diagnostics(IVD) Baiming Biotechnology Co.Ltd.Yancheng 224000China

Antisense RNA molecule represents a unique type of DNA transcript that comprises 19-23 nucleotides and is complementary to mRNA. Antisense RNAs play the crucial role in regulating gene expression at multiple levels, such as at replication, transcription, and translation. In addition, artificial antisense RNAs can effectively regulate the expression of related genes in host cells. With the development of antisense RNA, investigating the functions of antisense RNAs has emerged as a hot research field. This review summarizes our current understanding of antisense RNAs, particularly of the formation of antisense RNAs and their mechanism of regulating the expression of their target genes. In addition, we detail the effects and applications of antisense RNAs in antivirus and anticancer treatments and in regulating the expression of related genes in plants and microorganisms. This review is intended to highlight the key role of antisense RNA in genetic research and guide new investigators to the study of antisense RNAs.

关键词： Antisense RNA Formation mode Regulatory mechanism Application field Genetic research

Tuning Surface Molecular Design of Porous Carbon for Blue Energy Harvesting

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Research 2024年第1期2023卷 651-662页

作者： jian Yu zhong-Lin Wang Tianwei Ma Department of Civil and Environmental Engineering University of Hawaii at MānoaHonoluluHI 96822USA Beijing Institute of Nanoenergy and Nanosystems Chinese Academy of SciencesBeijing 101400China College of Engineering Texas A&M University-Corpus ChristiCorpus ChristiTX 78412USA

Capacitive mixing is a promising blue energy technology due to its membrane-free electricity generation and long electrode life ***,because of limited performance,existing systems do not lend themselves to practical *** it is a crucial factor directly influencing electrode behavior,surface chemistry has largely been overlooked in capacitive ***,we show that manipulating surface functionalization alone can tune the responses of electrodes to produce a high voltage rise without altering the pore structure of the *** findings reveal that the spontaneous electrode potential of a surface-modified carbon electrode shifts negatively proportional to the surface charge due to the surface groups,which explains why and how manipulating the surface chemistry can improve the power generation capacity.

关键词： electrode Porous mixing