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Study on the Simultaneously Quantitative Detection for β-Lactoglobulin and Lactoferrin of Cow Milk by Using Protein Chip Technique

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Biomedical and Environmental Sciences 2017年第12期30卷 875-886页

作者： YIN Ji yong HUO Jun Sheng ma Xin Xin SUN Jing HUANG jian National Institute for Nutrition and Health Chinese Center for Disease Control and Prevention Beijing 100050China

Objective To research a protein chip method which can simultaneously quantitative detect β-Lactoglobulin （β-L） and Lactoferrin （Lf） at one time. Methods Protein chip printer was used to print both anti-β-L antibodies and anti-Lf antibodies on each block of protein chip. And then an improved sandwich detection method was applied while the other two detecting antibodies for the two antigens were added in the block after they were mixed. The detection conditions of the quantitative detection for simultaneous measurement of β-L and Lf with protein chip were optimized and evaluated. Based on these detected conditions, two standard curves of the two proteins were simultaneously established on one protein chip. Finally, the new detection method was evaluated by using the analysis of precision and accuracy. Results By comparison experiment, mouse monoclonal antibodies of the two antigens were chosen as the printing probe. The concentrations of β-L and Lf probes were 0.5 mg/mL and 0.5 mg/mL, respectively, while the titers of detection antibodies both of β-L and Lf were 1：2,000. Intra- and inter-assay variability was between 4.88% and 38.33% for all tests. The regression coefficients of protein chip comparing with ELISA for β-L and Lf were better than 0.734, and both of the two regression coefficients were statistically significant （r = 0.734, t = 2.644, P = 0.038; and r = 0.774, t = 2.998, P = 0.024）. Conclusion A protein chip method of simultaneously quantitative detection for β-L and Lf has been established and this method is worthy in further application.

关键词： Protein chip Simultaneously Quantitative detection β-Lactoglobulin Lactoferrin

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CAOSA-extracted lignin improves enzymatic hydrolysis of cellulose

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Green Energy & Environment 2024年第7期9卷 1101-1111页

作者： Sen ma Zheng Li Jonathan Sperry Xing Tang yong Sun Lu Lin jian Liu Xianhai Zeng College of Energy State Key Laboratory of Physical Chemistry of Solid SurfacesXiamen UniversityXiamen361102China Fujian Engineering and Research Centre of Clean and High-valued Technologies for Biomass Xiamen Key Laboratory of Clean and High-valued Utilization of BiomassXiamen361102China Centre for Green Chemical Science University of AucklandAuckland1142New Zealand

The conversion of biomass into sugar platform compounds is very important for the biorefinery *** is essential to the biomass of the sugar platform,however,the lignin obtained by pretreatment,as a key part of lignocellulose,generally has a passive effect on the enzymatic hydrolysis of cellulose into *** this study,p-TsOH(p-toluenesulfonic acid),DES(Deep eutectic solvent)and CAOSA(cooking with active oxygen and solid alkali)pretreatment ways were used to fraction lignin from bamboo *** CAOSA treatment,the hydrolysis efficiency of the pulp was 95.57%.Moreover,the effect of different treatment methods on lignin properties was studied and the promotion effect of lignin was investigated by adding it to the cellulose enzymatic hydrolysis *** this work,the results showed that CAOSA-extracted lignin with lower D(1.31-1.25)had a better adsorption effect on the enzyme protein.p-TsOH-extracted lignin with a larger S/G ratio enhanced the inhibition of enzymatic *** addition,the presence of-COOHs in lignin could reduce its inhibitory effect on cellulose saccharification.

关键词： Biomass pretreatment CAOSA Cellulose hydrolysis Lignin Enzyme

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Numerical study of nonlinear interactions of bi-chromatic progressive deep-water waves

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Journal of Hydrodynamics 2021年第3期33卷 602-620页

作者： jian-jian Xie Yu-xiang ma Guo-hai Dong Xiao-zhou ma State Key Laboratory of Coastal and Offshore Engineering Dalian University of TechnologyDalian116023China

This paper proposes a 3-D non-hydrostatic free surface flow model with a newly proposed general boundary-fitted grid system to simulate the nonlinear interactions of the bi-chromatic deep-water gravity ***,the monochromatic bidirectional and bi-chromatic bidirectional waves of small wave steepness are successively simulated to verify the abilities of the numerical ***,a series of bi-chromatic progressive waves of moderate wave steepness and different crossing angles are simulated and analyzed in *** is found that if the crossing angle is close to or smaller than the resonant angle,apparent discrepancies are observed among the numerical results,the linear wave theory,and the steady third-order ***,the three solutions coincide *** analysis,it is concluded that the discrepancies are caused by the third-order quasi-resonant interactions between the bi-chromatic progressive *** interactions not only could modify the wave spectrum,but could also change the wave shape patterns.

关键词： Non-hydrostatic model nonlinear interactions bi-chromatic progressive waves quasi-resonant interactions

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Testing and modeling of frozen clay-concrete interface behavior based on large-scale shear tests

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Advances in Climate Change Research 2021年第1期12卷 83-94页

作者： HE Peng-Fei MU Yan-Hu ma Wei HUANG yong-Ting DONG jian-Hua State Key Laboratory of Frozen Soil Engineering Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou730000China School of Science Lanzhou University of TechnologyLanzhou730050China University of Chinese Academy of Sciences Beijing100049China Key Laboratory of Disaster Prevention and Mitigation in Civil Engineering of Gansu Province Lanzhou University of TechnologyLanzhou730050China

The shear behavior of the frozen soil-structure interface is important for accurately predicting the interface responses of structures adopted in the cold *** purpose of this study is to experimentally and theoretically investigate the shear behavior of frozen clay-concrete interface under engineering conditions.A large-scale direct shear apparatus with a temperature-controlled shear box is used to test the interface *** specimens consisting of a cement concrete block and frozen soil with initial water content ranging between 14.6%and 24.6%were prepared at different conditions of temperatures(15.4 to-9.8℃),shear rates(0.03-0.9 mm min^(-1)),and normal stresses(50-200 *** is found that the peak shear strength is linear developing with increasing of normal stress,initial water content,and *** increased from 67.7 to 133.3 kPa as the initial water content increased from 14.9%to 24.6%at temperature of-6.8 to-6.6℃,and it increased from 51.2 to 80.6 kPa with temperature decreasing from 15.4 to-9.8℃at initial water content of 14.6%-14.9%,furthermore it has a power law relationship with shear *** final vertical displacement increases with the decreasing temperature,and increasing initial water ***,it is slight or could be ignored at lower shear rates(e.g.0.03 mm min^(-1) and 0.15 mm min^(-1))and it is-0.25 mm and-0.28 mm at shear rate of 0.3 mm min^(-1) and 0.9 mm min^(-1),*** addition,the evolution of vertical displacement also varies with test condition,the growth rate at beginning increases with increasing initial water content and decreasing temperature or ice content,which is because of the ice film effects the particle ***,a disturbed state concept model combined with linear and nonlinear characteristics is developed to describe the interface shear *** disturbance D reflects the interface mechanical response and responds differently trend for different test conditions,increasing faster with i

关键词： Shear strength Frozen clay-concrete interface Disturbed state concept Constitutive model

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Photometric redshift estimation of galaxies with Convolutional Neural Network

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Research in Astronomy and Astrophysics 2020年第6期20卷 193-202页

作者： yong-Huan Mu Bo Qiu jian-Nan Zhang Jun-Cheng ma Xiao-Dong Fan School of Electronics and Information Engineering Hebei University of TechnologyTianjin 300401China CAS Key Laboratory of Optical Astronomy National Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China

The abundant photometric data collected from multiple large-scale sky surveys give important opportunities for photometric redshift ***,low accuracy is still a serious issue in the current photometric redshift estimation *** this paper,we propose a novel two-stage approach by integration of Self Organizing map(SOM)and Convolutional Neural Network(CNN)methods *** SOM-CNN method is tested on the dataset of 150000 galaxies from Sloan Digital Sky Survey Data Release 13(SDSS-DR13).Inthe first stage,we apply the SOM algorithm to photometric data clustering and divide the samples into early-type and *** the second stage,the SOM-CNN model is established to estimate the photometric redshifts of ***,the precision rate and recall rate curves(PRC)are given to evaluate the models of SOM-CNN and Back Propagation(BP).It can been seen from the PRC that the SOM-CNN model is better than BP,and the area of SOM-CNN is 0.94,while the BP is ***,we provide two key error indicators:mean square error(MSE)and *** results show that the MSE of early-type is 0.0014 while late-type is 0.0019,which are better than the BP algorithm 22.2%and 26%,*** compared with Outliers,our result is optimally 1.32%,while the K-nearest neighbor(KNN)algorithm has 3.93%.In addition,we also provide the error visualization figures aboutΔZ andδ.According to the statistical calculations,the early-type with an error of less than 0.1 accounts for 98.86%,while the late-type is 99.03%.This result is better than those reported in the literature.

关键词： galaxies distances and redshifts-techniques photometric-method data analysis

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Deciphering the role of ultra-low-loaded rhodium in NiFe-MIL-53 for superior oxygen evolution reaction

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Journal of Energy Chemistry 2025年第1期100卷 77-86页

作者： Jinzhi Jia Jinhua Zhang Kailu Guo Lanyue Zhang Gening Du Hao You Junfeng Huang Mudong Tu Hua Li yong Peng Wei Dou Cailing Xu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering School of Materials and Energy Lanzhou University College of Chemistry and Chemical Engineering Luoyang Normal University

Designing highly active and stable electrocatalysts of oxygen evolution reaction （OER） is one of the crucial *** this study,a novel OER electrocatalyst,NiFe-MIL-53 modified with ultra-low rhodium（Rh@NiFe-MIL-53）,is successfully prepared via the hydrothermal ***-situ Raman spectroscopy and electrochemical impedance spectroscopy reveal that the doped Rh accelerates the phase transformation of NiFe-MIL-53 and the in-situ formed Rh@NiFeOOH is the actual active *** importantly,the enhanced reversibility of electrochemical reconstruction between NiFeOOH and NiFe（OH）2after doping Rh is beneficial for improving the electrochemical stability of the catalyst.X-ray photoelectron spectroscopy spectra show the strong electronic interaction between single-atom Rh and Ni/Fe in Rh@***,theoretical calculations confirm that the integration of single-atom Rh into the NiFeOOH successfully reduces the band gap,regulates the d-band center （εd）,accelerates the charge transfer,and optimizes the adsorption behavior of oxygen-containing intermediates,thereby lowering the energy barrier of rate-determining ***,the optimized Rh@NiFe-MIL-53 exhibits excellent OER activity （240 mV） with a small Tafel slope of 48.2 mV dec-1and long-term durability (over1270 h at 10 m A cm-2and 110 h at 200 ma cm-2).This work presents a new perspective on designing highly efficient OER electrocatalysts.

关键词： X ray photoelectron spectroscopy

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芝麻叶软包装罐头的研制

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食品安全质量检测学报 2017年第8期8卷 3156-3160页

作者：李和平魏建春马永生王斌申晓琳河南牧业经济学院食品工程学院郑州450046

目的研发以新鲜芝麻叶为原料制作芝麻叶软包装罐头。方法以新鲜芝麻叶为原料,通过单因素试验和正交设计法优化芝麻叶软包装罐头的加工工艺,研发出一种新型芝麻叶软包装罐头。结果芝麻叶软包装罐头最佳工艺参数为:105℃高温蒸制时间30min... 详细信息

目的研发以新鲜芝麻叶为原料制作芝麻叶软包装罐头。方法以新鲜芝麻叶为原料,通过单因素试验和正交设计法优化芝麻叶软包装罐头的加工工艺,研发出一种新型芝麻叶软包装罐头。结果芝麻叶软包装罐头最佳工艺参数为:105℃高温蒸制时间30min后,添加食盐为2.0%、辣椒粉3.0%、花椒油2.0%,腌制24h后,真空包装,杀菌即为成品。结论本研究研发出一种优良风味的罐藏芝麻叶食品,满足了人们对新型功能食品的需求,推动了罐藏绿色食品的发展。

关键词：芝麻叶罐头软包装食品正交设计法工艺优化

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Good Linear Codes from Quadratic Residue Codes over a Finite Non-Chain Ring

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Chinese Journal of Electronics 2017年第4期26卷 773-777页

作者： GAO jian ma Fanghui School of Science Shandong University of Technology

This paper is devoted to the study of quadratics residue codes and their extended codes over a finite non-chain ring. A class of Gray maps preserving the self-duality from the ring to the finite field are *** structural properties of quadratic residue codes and their extended codes are given. As an interesting application of these families of codes, some good linear codes are obtained by a special Gray map.

关键词： Gray map Quadratic residue codes Good linear codes

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Photocatalytic activity of perovskite SrTiO_(3)catalysts doped with variable rare earth ions

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Rare Metals 2021年第5期40卷 1077-1085页

作者： Yue-Xin Song Wen-Qi ma Jing-Jing Chen Jiao Xu Zhi-yong mao Da-jian Wang Tianjin Key Laboratory for Photoelectric materials and Devices School of Materials Science and EngineeringTianjin University of TechnologyTianjin 300384China Key Laboratory of Display materials and Photoelectric Devices Ministry of EducationTianjin University of TechnologyTianjin 300384China

In this work,15 types of rare earth(Re)ions,including Y^(3+),La^(3+),Ce^(3+),Pr^(3+),Nd^(3+),Sm^(3+),Eu^(3+),Gd^(3+),Tb^(3+),Dy^(3+),Ho^(3+),Er^(3+),Tm^(3+),Yb^(3+)and Lu^(3+)doped perovskite SrTiO_(3)powders were synthesized by solgel *** influence of Re ions doping on the crystal structure,morphology and optical property as well as the photocatalytic activity for the photodegradation of rhodamine B(RhB)was investigated in detail when the synthesized Re ions doped SrTiO_(3)powders were served as *** presented results revealed that the crystal structure is invariable,whereas the morphology and the optical bandgap are variable for the resultant SrTiO_(3)powders when different Re ions were incorporated into the SrTiO_(3)*** relatedness between the morphology,optical property and photocatalytic activity of the synthesized SrTiO_(3)catalysts doped with variable Re ions were analyzed deeply,providing an insight into the influence factors on the photocatalytic activity of catalysts.

关键词： SrTiO_(3) Rare earth ions Doping Photocatalytic activity

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Inhibitory effects of chloroquine on the activation of plasmacytoid dendritic cells in SIVmac239-infected Chinese rhesus macaques

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Cellular & Molecular Immunology 2012年第5期9卷 410-416页

作者： jian-Ping ma Hou-Jun Xia Gao-Hong Zhang jian-Bao Han Li-Guo Zhang yong-Tang Zheng Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences&Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunmingChina Graduate University of the Chinese Academy of Sciences BeijingChina CAS Key Laboratory of Infection and Immunity Institute of BiophysicsChinese Academy of SciencesBeijingChina

It is currently widely accepted that immune activation in HIV-infected individuals leads to a severe loss of CD4＋ T cells and the progression to AIDS. However, the underlying mechanism of this immune activation remains unclear. Experimental data suggest that the activation of plasmacytoid dendritic cells （pDCs） by plasma viremia may play a critical role in HIV-induced immune activation. In this study, we found that the level of immune activation was higher in the late phase of SIVmac239 infection compared with chronic infection, which suggests that immune activation might be related to disease progression in SIVmac239-infected non-human primate models. Our work also showed that chloroquine could effectively inhibit the activation of pDCs in vitro and in vivo. However, chloroquine treatment of SIVmac239-infected macaques had no significant influence on the Cellular composition of peripheral blood in these animals.

关键词： Chinese rhesus macaque chloroquine immune activation pDC SIV

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