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邢台职业技术学院学报 2019年第1期36卷 101-104页

作者：弋建伟中铁十八局集团第四工程有限公司天津300350

文章首先对铁路经营管理模式进行阐述,从职责分布不明确、管理方式不合理、管理人员素养有待提升三个方面入手,对铁路建筑项目管理存在的问题进行解析,并结合工程案例,提出地方铁路运营管理模式的方案选择对策。

关键词：铁路经营管理模式方案选择

High-yield expression of recombinant SARS coronavirus nudeocapsid protein in methylotrophic yeast Pichia pastoris

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World Journal of Gastroenterology 2004年第24期10卷 3602-3607页

作者： Ru-Shi Liu Kun-Yu Yang jian Lin yi-wei Lin Zhi-Hong Zhang Jun Zhang Ning-Shao Xia The Key Laboratory for Cell Biology and Tumor Cell Engineering of the Ministry of Education The Research Center for Medical Molecular Virology of Fujian Province,Xiamen University,Xiamen 361005,Fujian Province,China

AIM: Nucleocapsid (N) protein plays an important role in reproduction and pathological reaction of severe acute respiratory syndrome (SARS) coronavirus (SCoV), the antigenicity of the protein is better than spike (S) *** study was to find a highly specific and antigenic recombinant SCoV nucleocapsid (rSCoVN) protein, and to provide a basis for further researches on early diagnosis of ***: Full length cDNA of SCoV nucleocapsid (SCoVN) protein was amplified through polymerase chain reaction (PCR) and cloned into yeast expression vector pPIC3.5K to construct plasmid of pPIC3.5K-SCoVN. The plasmid was linearized and then transformed into Pichia pastoris (***) GS115 (His^+ Mut^+) by electroporation. His^+Mut^+ recombinant strains were identified by PCR and cultivated on MM/MD plates. The influence of different factors on biomass and rSCoVN protein production during induction phase, such as various induction media, dissolved oxygen (DO) and different final concentrations of methanol, was subsequently studied. The expression level and activation were detected by SDS-PAGE and Western-blot ***: All of the recombinants were His^+Mut^+ after transformation of *** with linearized plasmids. The BMMY medium was optimal for recombinant ScoVN (rSCoVN) protein expression and growth of the recombinant *** final optimal concentration of methanol was 20 ml_/L,the DO had a significant effect on rSCoVN protein expression and growth of recombinant strains. The rSCoVN protein expressed in recombinant strains was about 8% of the total cell protein, 520 mg/L of rSCoVN protein was achieved,and a maximum cell A at 600 nm of 62 was achieved in shake flask culture. The rSCoVN protein had a high specificity against mouse-anti-SARS-CoVN-mAb and SARS positive sera, but had no cross-reaction with normal human *** biological activity of rSCoVN expressed in *** was about 4-fold higher than that expressed in Ecoliwhen the same rSCoVN

关键词：高生长表达重组细胞 SARS 冠状病毒核壳体蛋白甲基营养菌毕赤酵母 rSCoVN蛋白质

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窄光束在掠反射条件下的特性

物理学报 2008年第7期57卷 4208-4213页

作者：余辉江晓清杨建义戚伟王明华浙江大学信息与电子工程学系杭州310027

在掠入射条件下,由于部分平面波分量无法直接入射到反射面,从而不能按照常规方式被反射和透射.而反射透射模式的逆过程被用来描述非直接入射的这部分平面波分量与反射区的相互作用.基于这两个模式,采用平面波展开法导出了窄光束掠入射... 详细信息

在掠入射条件下,由于部分平面波分量无法直接入射到反射面,从而不能按照常规方式被反射和透射.而反射透射模式的逆过程被用来描述非直接入射的这部分平面波分量与反射区的相互作用.基于这两个模式,采用平面波展开法导出了窄光束掠入射条件下出射光的场分布,理论计算结果与采用BPM方法的模拟值完全一致.

关键词：掠反射平面波展开法导波模式光波导开关

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基于校园网的实验室管理运行方案探索

陕西师范大学学报（自然科学版） 2008年第S2期36卷 180-181页

作者：任丹尚玉梅弋建伟陕西职业技术学院陕西西安710100

高职院校实验室传统的管理体制和模式的改革已是很迫切的要求。作者通过分析,认为在建立新的管理体制的同时,借助现代网络和计算机技术,利用基于校园网的实验室管理系统,更好地贯彻实验室体制改革的思想,为提高实验室管理水平探索可行... 详细信息

高职院校实验室传统的管理体制和模式的改革已是很迫切的要求。作者通过分析,认为在建立新的管理体制的同时,借助现代网络和计算机技术,利用基于校园网的实验室管理系统,更好地贯彻实验室体制改革的思想,为提高实验室管理水平探索可行的方案。

关键词：实验室管理改革校园网资源共享

Mesospheric tide comparisons at low latitudes observed by two collocated meteor radars

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Earth and Planetary Physics 2025年第1期9卷 54-68页

作者： jian Li Wen yi XiangHui Xue Jie Zeng HaiLun Ye jianYuan Wang JinSong Chen Na Li TingDi Chen Chinese Academy of Sciences(CAS)Key Laboratory of Geospace Environment Department of Geophysics and Planetary SciencesUniversity of Science and Technology of ChinaHefei 230026China CAS Center for Excellence in Comparative Planetology Anhui Mengcheng Geophysics National Observation and Research StationUniversity of Science and Technology of ChinaHefei 230026China Hefei National Laboratory University of Science and Technology of ChinaHefei 230026China Collaborative Innovation Center of Astronautical Science and Technology Harbin 150001China National Key Laboratory of Electromagnetic Environment China Research Institute of Radiowave PropagationQingdao 266107China Yunnan Kunming Electromagnetic Environment National Observation and Research Station Qujing Kunming 655000China

Accurate knowledge of mesospheric winds and waves is essential for studying the dynamics and climate in the mesosphere and lower thermosphere(MLT)*** this study,we conduct a comparative analysis of the mesosphere tidal results obtained from two adjacent meteor radars at low latitudes in Kunming,China,from November 2013 to December *** two radars operate at different frequencies of 37.5 MHz and 53.1 MHz,***,overall good agreement is observed between the two radars in terms of horizontal winds and tide *** results show that the dominant tidal waves of the zonal and meridional winds are diurnal and semidiurnal ***,we conduct an exhaustive statistical analysis to compare the tidal amplitudes and vertical wavelengths recorded by the dual radar systems,which reveals a high degree of alignment in tidal *** investigation includes variances and covariances of tidal amplitudes,which demonstrate remarkable consistency across measurements from both *** finding highlights clear uniformity in the mesospheric tidal patterns observed at low latitudes by the two neighboring meteor *** of the comparative analysis specifically underscore the significant correlation in vertical wavelength measurements,validating the robustness of radar observations for tidal research.

关键词： mesosphere and lower thermosphere region meteor radar mesospheric winds tides

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直列无级调压式速卸锚索施工技术

施工技术 2007年第9期36卷 37-39页

作者：李保国宁锐林建平易建伟李小川北京力川地基工程有限公司北京100070 中国中铁一局集团有限公司陕西西安710054 北京市市政工程设计研究总院北京100045

结合工程实例,从基坑支护方案选择,锚索拆除方案设计,锚索施工等几个方面介绍了直列无极调压式速卸锚索的施工技术。最后通过锚索试验确定了承载体之间的合理距离,并对夹具的可靠性做了测试。

关键词：基坑支护速卸锚索压力分散型

Experimental demonstration of 220-GHz terahertz signals wireless transmission over 4.6 km

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Science China(Information Sciences) 2025年第1期68卷 386-388页

作者： yi wei jianjun YU Mingxu WANG Xiongwei YANG Qiutong ZHANG Jingwen TAN Bing ZHANG ying WU Yang HAN Peng TIAN Wen ZHOU Kaihui WANG Feng ZHAO State Key Laboratory of ASIC and System Key Laboratory for Information Science of Electromagnetic Waves (MoE)School of Information Science and Technology Fudan University School of Electronic Engineering Xi'an University of Posts and Telecommunications

The advancement of terahertz (THz) communication technology drives the evolution of wireless communication systems,offering novel pathways and technical means for the development of future 6G communication *** wireless communication systems are often constrained by bandwidth limitations of electronic devices in high frequency ***,THz communication technology leverages the characteristics of electromagnetic waves to transcend these limitations,enabling communication athigher frequencies and wider bandwidths.

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Unlocking the future:Mitochondrial genes and neural networks in predicting ovarian cancer prognosis and immunotherapy response

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World Journal of Clinical Oncology 2025年第1期16卷 43-52页

作者： Zhi-jian Tang Yuan-Ming Pan wei Li Rui-Qiong Ma jian-Liu Wang Department of Obstetrics and Gynecology Peking University People’s HospitalBeijing 100044China Cancer Research Center Beijing Chest HospitalBeijing 101149China Department of Thoracic Surgery Sichuan Provincial People's HospitalChengdu 610072Sichuan ProvinceChina

BACKGROUND Mitochondrial genes are involved in tumor metabolism in ovarian cancer(OC)and affect immune cell infiltration and treatment *** To predict prognosis and immunotherapy response in patients diagnosed with OC using mitochondrial genes and neural *** Prognosis,immunotherapy efficacy,and next-generation sequencing data of patients with OC were downloaded from The Cancer Genome Atlas and Gene Expression *** genes were sourced from the MitoCarta3.0 *** discovery cohort for model construction was created from 70% of the patients,whereas the remaining 30% constituted the validation *** the expression of mitochondrial genes as the predictor variable and based on neural network algorithm,the overall survival time and immunotherapy efficacy(complete or partial response)of patients were *** In total,375 patients with OC were included to construct the prognostic model,and 26 patients were included to construct the immune efficacy *** average area under the receiver operating characteristic curve of the prognostic model was 0.7268[95% confidence interval(CI):0.7258-0.7278]in the discovery cohort and 0.6475(95%CI:0.6466-0.6484)in the validation *** average area under the receiver operating characteristic curve of the immunotherapy efficacy model was 0.9444(95%CI:0.8333-1.0000)in the discovery cohort and 0.9167(95%CI:0.6667-1.0000)in the validation *** The application of mitochondrial genes and neural networks has the potential to predict prognosis and immunotherapy response in patients with OC,providing valuable insights into personalized treatment strategies.

关键词： Ovarian cancer Mitochondria Prognosis Immunotherapy Neural network

核心结合因子和Ⅱ型胶原在慢性肾脏病5期患者桡动脉中的表达

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中华肾脏病杂志 2012年第11期28卷 868-872页

作者：余毅颜开萍王琰孙淑清陈今林开平易建伟南京军区福州总医院血液净化科福州350025

目的探讨慢性肾脏病（CKD）5期患者桡动脉中核心结合因子（Cbfα-1）和Ⅱ型胶原（ColⅡ）表达及其与血管钙化发生的关系。方法40例CKD5期患者在接受动-静脉内瘘手术时，留取一段桡动脉。对10例在本院接受胃大部切除术、肾功能正常患者... 详细信息

目的探讨慢性肾脏病（CKD）5期患者桡动脉中核心结合因子（Cbfα-1）和Ⅱ型胶原（ColⅡ）表达及其与血管钙化发生的关系。方法40例CKD5期患者在接受动-静脉内瘘手术时，留取一段桡动脉。对10例在本院接受胃大部切除术、肾功能正常患者，留取一段胃左动脉为对照组。钙盐沉积采用von Kossa染色法，根据yonKossa染色结果，CKD5期组又分为CKD5期无钙化组、轻中度钙化组和重度钙化组。Cbfα-1和ColⅡ的表达采用免疫组化染色法。同时测定血钙、磷、甲状旁腺激素（iPTH）、C反应蛋白（CRP）水平。结果40例CKD5期患者中有17例（42．5％）出现血管钙化，主要发生在血管中膜，对照组均未见血管钙化发生。CKD5期轻中度钙化组和重度钙化组中，动脉中膜均有Cbfα-1、ColⅡ的阳性表达；23例CKD5期无钙化组中，有18例（78．3％）至少有Cbfα-1、ColⅡ其中一种的阳性表达。对照组均无Cbfα-1、Col Ⅱ的阳性表达。CKD5期轻中度钙化组和重度钙化组，血管中膜Cbfα-1、ColⅡ免疫组化的阳性率均显著高于CKD5期无钙化组，但轻中度钙化组和重度钙化组之间的差异无统计学意义。CRP、钙磷乘积与Cbfα-1、ColⅡ呈正相关；血磷与Cbfα-1（r=0．786，P〈0．01）、ColⅡ（r=0．785，P〈0．01）呈正相关。结论CKD5期患者桡动脉钙化以中膜为主，钙化的动脉均有Cbfα-1、ColⅡ的高表达，且其表达早于组织学上血管钙化的发生。Cbfα-1和ColⅡ可能在血管钙化的发生、发展中起到了重要作用。

关键词：核心结合因子α1亚基胶原Ⅱ型肾功能不全慢性血管钙化高血磷

Constructing 2D bismuth-based heterostructure for highly efficient photocatalytic CO2 reduction

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Tungsten 2025年第1期7卷 71-79页

作者： Ruo-Hang Xu Hao-Yu jiang Dan-Dan Cui Kun-Rong Du Hong-Run Zhang Sheng-Jie Xu Ya-Qi Li Zhi-Zhen Ren Liang Wang wei-Chang Hao yi Du School of Physics and BUAA-UOW Joint Research Centre Beihang University Shenzhen Institute of Beihang University Centre for Catalysis and Clean Energy School of Environment and Science Griffith University

Reforming carbon dioxide（CO2） into fuels and valuable chemicals using solar energy holds significant potential for emission reduction and addressing energy supply *** recombination of photocarriers remains a major obstacle,however,to enhancing solar energy conversion efficiency in the photocatalytic reaction *** two-dimensional materials,constructing heterogeneous structures has emerged as an effective strategy to enhance the separation of photoexcited carriers by leveraging the internal electric field at the heterogeneous ***,we present a facile strategy for preparing Bi4NbO8Br/Bi2WO6plate-on-plate Z-scheme junctions with a strong internal electric field by in situ *** unique heterostructure facilitates efficient separation and transport of photoexcited charges by harnessing the internal electric field induced by chemical bonding at the lateral ***,the Bi4NbO8Br/Bi2WO6heterostructure exhibits enhanced CO2photoreduction properties,along with a CO yield rate of approximately 35 μmol·g-1·h-1under full light *** work offers novel insights into the synthesis of unique hetero structures intended to enhance the efficiency of photocatalytic CO2reduction.

关键词： Bi4NbO8Br/Bi2WO6 CO2 reduction Heterostructures Photocatalysts