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Nitrogen interception in floodwater of rice field in Taihu region of China

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Journal of Environmental Sciences 2007年第12期19卷 1474-1481页

作者： LIANG Xin-qiang CheN Ying-xu LI Hua TIAN Guang-ming ZHANG Zhi-jian NI wu-zhong he Miao-miao Department of Environmental Engineering College of Natural Resources and Environmental Science Zhejiang University Hangzhou 310029 China.

A field experiment located in Taihu Lake Basin of China was conducted, by application of urea or a mixture of urea with manure, to elucidate the interception of nitrogen （N） export in a typical rice field through ＂zero-drainage water management＂ combined with sound irrigation, rainfall forecasting and field drying. N concentrations in floodwater rapidly declined before the first event of field drying after three split fertilizations, and subsequently tended to rearm to the background level. Before the first field drying, total particulate nitrogen （TPN） was the predominant N form in floodwater of plots with no N input, dissolved inorganic nitrogen （DIN） on plots that received urea only, and dissolved organic nitrogen （DON） on plots treated with the mixture of urea and manure. Thereafter TPN became the major form. No N export was found from the rice field, but total nitrogen （TN） of 15.8 kg/hm^2 was remained, mainly due to soil N sorption. The results recommended the zero-drainage water management for full-scale areas for minimizing N export.

关键词： nitrogen paddy field surface floodwater interception Taihu region

Relationship between expression of gastrin, somatostatin,Fas/FasL and caspases in large intestinal carcinoma

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World Journal of Gastroenterology 2008年第18期14卷 2802-2809页

作者： Jia-Ding Mao Pei wu Ying-Lin Yang jian wu he Huang Department of General Surgery the First Affiliated Yijishan Hospital of Wannan Medical College Wuhu 241001 Anhui Province China

AIM: To explore the correlation between the mRNAs and protein expression of gastrin (GAS), somatostatin (SS) and apoptosis index (AI), apoptosis regulation gene Fas/ FasL and caspases in large intestinal carcinoma (LIC). METHODS: Expression of GAS and SS mRNAs were detected by nested RT-PCR in 79 cases of LIC. Cell apoptosis was detected by molecular biology in situ apoptosis detecting methods (TUNEL). Immunohistochemical staining for GAS, SS, Fas/FasL, caspase-3 and caspase-8 was performed according to the standard streptavidin-biotin-peroxidase (S-P) method. RESULTS: There was a significant positive correlation between mRNA and protein expression of GAS and SS (GASrs=0.99, P here was significant difference in positive expression rates of GAS, SS mRNAs and protein among different histological differentiation, histological types and Dukes’ stage of LIC. The AI in GAS high and moderate expression groups was significantly lower than that in low expression groups (3.75 ± 2.38 vs 7.82 ± 2.38, P he AI in SS high and moderate expression groups was significantly higher than that in low expression groups (9.03 ± 1.76 vs 5.35 ± 3.00, P here was a significant negative correlation between the integral ratio of GAS to SS and the AI (rs = -0.41, P he positive expression rate of FasL in GAS high and moderate expression groups was higher than thatin low expression group (90.9% and 81.0% vs 53.2%, P he positive expression rates of Fas, caspase-8 and caspase-3 in SS high (90.0%, 90.0% and 100%) and moderate (80.0%, 70.0%, 75.0%) expression groups were higher than that in low expression group (53.1%, 42.9%, 49.0%) (90.0% and 80.0% vs 53.1%, P here was a significant positive correlation between the integral ratio of GAS to SS and the semiquantitative in

关键词： Large intestinal carcinoma Gastrin Somatostatin Apoptosis index Fas FasL Caspase

Botanical drugs for bruises used in the Xiangxi region of China, a place rich in martial culture

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Traditional Medicine Research 2023年第11期8卷 45-54页

作者： jian-wu he Hong-Tao Gao Xuan Liu Qiang Li Jin Luo Jin Yan Bin-Sheng Luo National and Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia Ulmoides Jishou UniversityJishou 416000China College of Biology and Environmental Science Jishou UniversityJishou 416000China Lushan Botanical Garden Jiangxi Province and Chinese Academy of SciencesLushan 332900China.

Background:Xiangxi Tujia and Miao Autonomous Prefecture,located in Hunan Province,China,is a culturally rich region with a diverse *** locals have a deep martial arts history and possess a wealth of traditional knowledge for treating ***:In order to investigate this topic,we conducted ethnobotanical studies by visiting local medicinal markets and conducting ***:We identified 43 botanical drugs used for treating bruises,categorized into four types:sprains,contusions,strains,and bone *** local people typically prefer to mash these botanical drugs freshly or make medicinal wine for external *** often involves a combination of drugs to promote blood circulation and remove blood stasis,with medications to dispel rheumatism,clear heat,and detoxify,tailored to the humid climate and high precipitation in mountainous *** on the fidelity level values,the local plant resources with high acceptance,utilization,or potential value in the treatment of bruises included Sargentodoxa cuneata,Lycopodium japonicum,Dioscorea cirrhosa,Panax japonicus,Achyranthes bidentata,Lysimachia heterogenea and so ***:herbal medicine and related knowledge for treating bruises are diverse and rich in the Xiangxi ***,the local traditional medicinal knowledge faces challenges regarding inheritance due to regional ***,it is crucial to protect and further study this knowledge in the future.

关键词： bruises medicinal plants ethnobotany Xiangxi traditional knowledge

Application of GP5 Protein to Develop Monoclonal Antibody against Porcine Reproductive and Respiratory Syndrome Virus

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Virologica Sinica 2011年第4期26卷 267-272页

作者： Hong Tian Yan Cheng Jin-yang wu jian-hui he You-jun Shang Xiang-tao Liu State Key Laboratory of Veterinary Etiological Biology National Foot and Mouth Disease Reference Laboratory Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046 China

In this study, a panel of monoclonal antibodies （mAbs） against Porcine reproductive and respiratory syndrome virus（PRRSV）, named as 8C9 and4B4, were produced by fusing SP2/0 myeloma cells and spleen cells of BALB/c mice immunized with the PRRSV （TCID50=5.5）, screened by the indirect ELISA and subjected to several limiting dilutions, mAbs were then identified by biological characterization. Among the two fusion cell strains, 8C9 belonged to the IgG1 subclass and 4B4 belonged to the IgG2a subclass. The titers in cell culture supematant and abdomen liquor reached to 1：104and 1：105, respectively. The specificity test indicated that the two cells had specific reactions for the PRRSV and GP5 protein respectively, and no reaction with Classical swine fever virus （CSFV） or Swine vesicular disease virus （SVDV）. The molecular weights of the heavy chain and light chain were about 45.0 kDa and 25.0 kDa, respectively. In neutralization activity tests, the results showed that the prepared mAb 4B4 can protect 50% of cells with no CPE in dilution up to 1：512, but mAB 8C9 has no neutralization activities to PRRSV.

关键词： Porcine reproductive and respiratory syndrome virus (PRRSV) Recombinant GP5 protein Monoclonal antibody

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离子色谱法测定盐酸伐昔洛韦中的氯离子

中国新药杂志 2017年第3期26卷 271-273页

作者：刘朝霞李婕吴健敏何兰中国食品药品检定研究院北京100050

目的:建立离子色谱法测定盐酸伐昔洛韦中氯的含量。方法:采用抑制型离子色谱法,Dionex Ion Pac AS18(250 mm×4 mm,5μm)阴离子交换色谱柱,以30 mmol·L^(-1)氢氧化钾溶液为淋洗液,流速为1.0m L·min^(-1),检测池温度为35℃,电导检测器... 详细信息

目的:建立离子色谱法测定盐酸伐昔洛韦中氯的含量。方法:采用抑制型离子色谱法,Dionex Ion Pac AS18(250 mm×4 mm,5μm)阴离子交换色谱柱,以30 mmol·L^(-1)氢氧化钾溶液为淋洗液,流速为1.0m L·min^(-1),检测池温度为35℃,电导检测器。结果:氯离子与常见成盐阴离子分离良好,在2~30μg·m L^(-1)浓度范围内呈良好的线性关系(r=0.999 9);定量限为0.8μg·m L^(-1);加样回收率为99.1%(n=9,RSD为1.3%)。结论:该方法准确、灵敏、专属性好,可用于测定盐酸伐昔洛韦中氯的含量,以控制成盐比例。

关键词：离子色谱盐酸伐昔洛韦氯离子含量成盐比例

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基于磁定位技术的滑坡监测试验研究

长江科学院院报 2017年第3期34卷 45-49页

作者：陆丹陈池吴剑何建伟周蕊三峡大学土木与建筑学院湖北宜昌443002

滑坡的监测主要包括2个方面,即地表位移监测和深部位移监测。深部位移监测能够直观反映滑坡的内部状态,是滑坡监测技术的研究重点。针对目前深部位移监测技术存在的诸多问题,提出了一种新型的滑坡深层位移监测技术,即一维磁定位技术。... 详细信息

滑坡的监测主要包括2个方面,即地表位移监测和深部位移监测。深部位移监测能够直观反映滑坡的内部状态,是滑坡监测技术的研究重点。针对目前深部位移监测技术存在的诸多问题,提出了一种新型的滑坡深层位移监测技术,即一维磁定位技术。采用理论和试验相结合的分析手段,探索这一技术在滑坡深部位移监测中的可行性;通过对磁定位相关理论的分析,推导出了深部位移与磁感应强度之间的理论关系式,并采用试验验证了这一关系的正确性。最后随机选取了8个验证点进行定位验证试验,结果证明了一维磁定位技术在滑坡监测中的可行性,其定位精度能够达到mm级,满足滑坡监测精度要求。

关键词：滑坡监测磁定位技术磁偶极子永磁体磁感应强度

基于精细爆破的隧道微台阶开挖工法与工程应用

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铁道工程学报 2012年第1期29卷 57-61,82页

作者：马辉李杰左巧峰吴建和西南交通大学成都610031 中铁二局集团有限公司成都610032

研究目的:通过精细爆破技术和悬臂拼装式多功能台架应用,开发出一种适用于IV、V级石质围岩隧道施工的开挖新工法,以解决软弱围岩隧道开挖掌子面与仰拱等后继工序安全步距保持、进度保障等施工问题。研究结论:基于精细爆破技术和悬臂拼... 详细信息

研究目的:通过精细爆破技术和悬臂拼装式多功能台架应用,开发出一种适用于IV、V级石质围岩隧道施工的开挖新工法,以解决软弱围岩隧道开挖掌子面与仰拱等后继工序安全步距保持、进度保障等施工问题。研究结论:基于精细爆破技术和悬臂拼装式多功能台架的隧道微台阶开挖工法,有效地提高了软弱围岩隧道开挖施工效率,为后继工序施工提供了更大的作业空间,缩小了掌子面与仰拱、二衬之间的距离,有利于保障隧道施工安全和进度。

关键词：精细爆破悬臂拼装式多功能台架微台阶工艺工法

Insights into spinal muscular atrophy from molecular biomarkers

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Neural Regeneration Research 2025年第7期20卷 1849-1863页

作者： Xiaodong Xing Xinzhu Liu Xiandeng Li Mi Li Xian wu Xiaohui Huang Ajing Xu Yan Liu jian Zhang Department of Clinical Pharmacy Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghaiChina College of Pharmacy Chongqing Medical UniversityChongqingChina

Spinal muscular atrophy is a devastating motor neuron disease characterized by severe cases of fatal muscle *** is one of the most common genetic causes of mortality among infants aged less than 2 *** research is currently receiving more attention,and new candidate biomarkers are constantly being *** review initially discusses the evaluation methods commonly used in clinical practice while briefly outlining their respective pros and *** also describe recent advancements in research and the clinical significance of molecular biomarkers for spinal muscular atrophy,which are classified as either specific or non-specific *** review provides new insights into the pathogenesis of spinal muscular atrophy,the mechanism of biomarkers in response to drug-modified therapies,the selection of biomarker candidates,and would promote the development of future ***,the successful utilization of biomarkers may facilitate the implementation of gene-targeting treatments for patients with spinal muscular atrophy.

关键词： biomarkers disease progression gene-targeting therapy neurofilaments Nusinersen spinal muscular atrophy(SMA) survival motor neuron therapeutic evaluation treatment outcomes

Perilipin-2 mediates ferroptosis in oligodendrocyte progenitor cells and myelin injury after ischemic stroke

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Neural Regeneration Research 2025年第7期20卷 2015-2028页

作者： jian Yang jiang wu Xueshun Xie Pengfei Xia Jinxin Lu Jiale Liu Lei Bai Xiang Li Zhengquan Yu Haiying Li Department of Neurosurgery&Brain and Nerve Research Laboratory The First Affiliated Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina Institute of Stroke Research Soochow UniversitySuzhouJiangsu ProvinceChina

Differentiation of oligodendrocyte progenitor cells into mature myelin-forming oligodendrocytes contributes to *** of remyelination due to oligodendrocyte progenitor cell death can result in severe nerve *** is an iron-dependent form of regulated cell death caused by membrane rupture induced by lipid peroxidation,and plays an important role in the pathological process of ischemic ***,there are few studies on oligodendrocyte progenitor cell *** analyzed transcriptome sequencing data from GEO databases and identified a role of ferroptosis in oligodendrocyte progenitor cell death and myelin injury after cerebral *** analysis suggested that perilipin-2(PLIN2)was involved in oligodendrocyte progenitor cell ***2 is a lipid storage protein and a marker of hypoxia-sensitive lipid droplet *** further investigation,we established a mouse model of cerebral ischemia/*** found significant myelin damage after cerebral ischemia,as well as oligodendrocyte progenitor cell death and increased lipid peroxidation levels around the infarct *** ferroptosis inhibitor,ferrostatin-1,rescued oligodendrocyte progenitor cell death and subsequent myelin *** also found increased PLIN2 levels in the peri-infarct area that co-localized with oligodendrocyte progenitor ***2 knockdown rescued demyelination and improved neurological *** findings suggest that targeting PLIN2 to regulate oligodendrocyte progenitor cell ferroptosis may be a potential therapeutic strategy for rescuing myelin damage after cerebral ischemia.

关键词： bioinformatics bulk RNA sequencing ferroptosis ischemic stroke myelin injury oligodendrocyte progenitor cell perilipin-2 single-cell RNA sequencing